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The article discusses a study on the effect of para-JEM BLUE diagnostic reagent on Mycobacterium avium subspecies paratuberculosis (MAP), a major pathogen of cattle. MAP was inoculated into broth-based culture media and was evaluated through fecal samples with known MAP status. The study showed that when para-JEM BLUE was eliminated from the broth, false-positive instrument signals increased. It found that para-JEM BLUE led to growth retardation in certain MAP.

The article discusses a study on the differences among the methods for extracting Mycobacterium avium subspecies paratuberculosis DNA for polymerase chain reaction from broth-based culture systems. The methods used for extraction included MagMAX, DNeasy, and phenol-chloroform. Results showed the existence of bacterial DNA in 197 out of 304 samples tested using the MagMAX method.

The article discusses the notification to Wisconsin Division of Public Health on March 25, 2015 of a possible infection outbreak on respiratory syncytial virus (RSV) among the hospitalized infants in neonatal intensive care unit (NICU). Topics mentioned include the positive interpretation and use of rapid respiratory syncytial virus antigen detection test (RRADT), the false interpretation due to viral transport media that are contaminated, and the suggestion to use RRADT to symptomatic case.