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Copyright of Veterinary Journal of Ankara University / Ankara Universitesi Veteriner Fakultesi Dergisi is the property of Ankara University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Classical procedures for semen evaluation in breeding soundness examination require time and are costly. The evaluation techniques with CASA are limited due to the lack of standardization among laboratories and data analysis methods. Recent studies have contradictory conclusions on the actual predictive ability of this method alone regarding fertility. Finding a simple and objective test that can give the maximum correlation between in vitro and in vivo results has been the main focus of novel research. In this study, we aimed to develop a repeatable protocol of sperm adhesion test using oviductal explant (AOC) and the results were compared with computer-assisted semen analysis (CASA) parameters along with field fertility. After the developmental phase, AOC was applied to a group of endangered Burlina breed bulls in which effective field results are difficult to obtain due to the narrow range of the population. The AOC test was found to be proficient enough to provide a prediction on bull semen fertility. Counting the sperms adhered in three microscopic fields after a coincubation period of 20 min in phosphate buffered saline (PBS) can provide useful information on the field level of fertility. The AOC test gives additional information to complement the standard semen evaluation methods that can be applied to endangered breeds as well. [ABSTRACT FROM AUTHOR]

Prediction of male fertility with in vitro semen quality parameters remains a challenge for the bull industry. Fluorescein staining and computer-assisted semen analysis (CASA) ensure kinetically proper and functionally objective results to improve spermatological parameter control. Therefore, we aimed to examine the CASA kinetic parameters and fluorescein staining of cryopreserved bull semen and its relationship with fertility. A total of 90 frozen straws from 5 Simmental bulls were evaluated. After semen was thawed, the motility, kinetic parameters, and insemination doses were evaluated by CASA; fluorescein stainings were evaluated using a fluorescein microscope. The 60th-day nonreturn rates (NRRs) were recorded for determination of pregnancy rates. There were significant differences in motility and kinetic parameters among bulls. Bull 3 had the lowest percentage of NRR and curvilinear velocity, straight line velocity, beat-cross frequency, and lateral head displacement parameters (P < 0.05). A positive correlation was detected between the pregnancy rate and some kinetic parameters. The correlation between the CASA parameters was determined as well. We concluded that various kinetic parameters obtained with CASA software system algorithms and fluorescein stainings can be linked to fertility. However, further research is needed to acquire a more precise link with fertility. [ABSTRACT FROM AUTHOR]

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this research, post-thaw effects of bull semen extenders including different amounts of boron (sodium pentaborate) on total motility (TMOT), progressive motility (PMOT), kinetic parameters of spermatozoa (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plasma membrane and acrosome integrity (PMAI) and DNA fragmentation index (DFI) were evaluated. Eight ejaculates were examined from four different bulls. Each ejaculate was split in to five aliquots and extended with standard Tris extender (Control) and 0.4 g boron with 0.5 g glucose (Group 1) 0.5; 0.7; 0.9 g boron without glucose (Group 2, Group 3 and Group 4 respectively) were used as experimental groups. After thawing TMOT, PMOT, kinetic traits of spermatozoa, PMAI and DFI were determined. According to motility and progressive motility (66.04%, 50.79%), Group 1 had higher values than the others, but differences between groups were not statistically significant (P>0.05). The highest values of VSL, LIN, STR and WOB were determined (28.15 µm/s, 42.98%, 71.69%, 59.71%) in Group 1 (P<0.05). While other kinetic traits of spermatozoa were higher numerically in Group 1 than the other groups, differences between groups were not statistically significant (P>0.05). The highest PMAI value was determined as 60.84% in Group 1 (P>0.05). The DFI rates were found similar in all control and experimental groups (P>0.05). In conclusion, it was observed that the boron (sodium pentaborate) has positive effects on post-thaw movement and structural traits. In addition, boron has no detrimental effect on DNA fragmentation index in semen freezing. Thus, boron could be used as an alternative semen extender compound. Özet: Bu çalışmada, farklı dozlarda bor (sodyum pentaborat) içeren boğa sperması sulandırıcılarının çözüm sonu toplam motilite (TMOT), progresif motilite (PMOT), spermatozoa hareket parametreleri (VCL, VSL, VAP, LIN, STR, WOB, ALH, BCF), plazma membranı ve akrozom bütünlüğü (PMAI) ve DNA fragmentasyon indeksi (DFI) parametreleri üzerine etkisi değerlendirildi. Dört baş boğadan toplam 8 ejakülat incelendi. Her ejakülat 5 eşit parçaya bölündü ve kontrol grubu olarak standart Tris sulandırıcısıyla sulandırıldı. Deney grubu sulandırıcıları olarak 0,5 g glikoza ek olarak 0,4 g bor eklenmiş Tris sulandırıcısı (Grup 1) ve glikoz yerine 0.5; 0.7; 0.9 g bor eklenmiş Tris sulandırıcıları (Grup 2, Grup 3, Grup 4) kullanıldı. Spermalar çözdürüldükten sonra TMOT, PMOT, spermatozoa hareket parametreleri, PMAI ve DFI değerleri belirlendi. Toplam motilite ve progresif motilite verilerine göre (%66.04, %50.79) Grup 1 sulandırıcısının daha yüksek değerlerde olmasına rağmen gruplar arası farklılıklar istatistiki açıdan önemsiz bulundu (P>0.05). En yüksek VSL, LIN, STR ve WOB değerlerinin (28.15 µm/s, %42.98, %71.69, %59.71) Grup 1'de olduğu görüldü (P<0.05). Diğer hareket özelliklerinin de Grup 1'de diğer gruplara göre yüksek olduğu belirlenirken, gruplar arası farklılıklar istatistiki açıdan önemsiz bulundu (P>0.05). En yüksek PMAI değerinin Grup 1'de olduğu, DFI değerlerinin ise tüm gruplarda benzer olduğu sonucuna ulaşıldı (P>0.05). Sonuç olarak borun çözdürme işleminden sonra spermatozoanın kinetik ve yapısal özellikleri üzerinde yararlı etkileri olduğu ortaya konmuştur. Ek olarak DFI açısından zararlı bir etkisi olmadığı gözlemlenmiştir. Bu veriler değerlendirildiğinde borun (sodyum pentaborat) alternatif bir sperma sulandırıcısı bileşeni olarak kullanılabileceği sonucuna ulaşılmıştır. [ABSTRACT FROM AUTHOR]

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Ataturk University Journal of Veterinary Sciences is the property of Ataturk University Coordinatorship of Scientific Journals and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Turkish Journal of Veterinary & Animal Sciences is the property of Scientific and Technical Research Council of Turkey and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)