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A proof of principle biosensor for the Brucella abortus recognition onsite is presented. The system is based on a plasmonic optical fiber probe functionalized with an oriented antibody layer immobilized on a short polyethyleneglycol (PEG) interface through carbodiimide chemistry and protein G as an intermediate layer. The biosensor is inserted in a holder built in 3D printing technology, obtaining a custom holder useful for housing the sample to be measured and the equipment. The removable sensor chip is a low-cost Surface Plasmon Resonance (SPR) platform based on D-shaped plastic optical fibers (POFs), built-in in 3D printed connectors, used here for the first time to detect bacteria via a bio-receptor layer specific for its membrane protein. The performances of the biosensor in Brucella abortus recognition are tested by using two different SPR-POF probes combined with the same bio-receptor layer. The best sensor configuration has presented a sensitivity at low concentrations of one order of magnitude greater than the other. A limit of detection (LoD) of 2.8 bacteria/mL is achieved well competitive with other systems but without the need for amplification or special sample treatments. Specificity has been tested using Salmonella bacteria, and reproducibility, regenerability and stability are moreover evaluated. These experimental results pave the way for building an efficient and specific biosensor system for Brucella abortus detection onsite and in a few minutes. Moreover, the proposed POF-based SPR biosensor device, with respect to the already available technologies, could be a Point-of-care-test (POCT), simple to use, small-size and portable, low-cost, don't necessary of a microfluidic system, and can be connected to the Internet (IoT). [ABSTRACT FROM AUTHOR]

In this work, F e 2 O 3 was investigated as a doping agent for poly(methyl methacrylate) (PMMA) in order to enhance the plasmonic effect in sensors based on D-shaped plastic optical fibers (POFs). The doping procedure consists of immerging a premanufactured POF sensor chip in an iron (III) solution, avoiding repolymerization and its related disadvantages. After treatment, a sputtering process was used to deposit a gold nanofilm on the doped PMMA in order to obtain the surface plasmon resonance (SPR). More specifically, the doping procedure increases the refractive index of the POF's PMMA in contact with the gold nanofilm, improving the SPR phenomena. The doping of the PMMA was characterized by different analyses in order to determine the effectiveness of the doping procedure. Moreover, experimental results obtained by exploiting different water–glycerin solutions have been used to test the different SPR responses. The achieved bulk sensitivities confirmed the improvement of the plasmonic phenomenon with respect to a similar sensor configuration based on a not-doped PMMA SPR-POF chip. Finally, doped and non-doped SPR-POF platforms were functionalized with a molecularly imprinted polymer (MIP), specific for the bovine serum albumin (BSA) detection, to obtain dose-response curves. These experimental results confirmed an increase in binding sensitivity for the doped PMMA sensor. Therefore, a lower limit of detection (LOD), equal to 0.04 μM, has been obtained in the case of the doped PMMA sensor when compared to the one calculated for the not-doped sensor configuration equal to about 0.09 μM. [ABSTRACT FROM AUTHOR]

Despite being a common issue in people with multiple sclerosis (pwMS), sexual dysfunction is still underinvestigated. This work aims to assess the potential determinants of sexual dysfunction in pwMS by considering its relationship with disease severity (in terms of global disability), illness perception, and depressive symptoms. In this multicenter study, 1010 pwMS responded to an online survey. A serial mediation model considering negative illness perception and depressive symptoms as mediators of the relationship between disease severity and sexual dysfunction was conducted using the SPSS PROCESS Macro with bias-corrected bootstrapping (5000 samples). Disease severity exerts an indirect effect on sexual dysfunction via illness perception, both independently and through depressive symptoms. However, the results indicated that illness perception plays a more crucial role in sexual dysfunction in pwMS with mild disability than in pwMS with moderate-severe disability. This study suggests that higher disability increases its magnitude by enhancing negative illness perception, that, in turn, affects sexual dysfunction both directly and through depressive symptoms, especially in pwMS with mild disability. Modulating the effect of illness perception by favoring adaptive coping strategies might represent a valid approach to mitigate sexual dysfunction symptoms in MS. [ABSTRACT FROM AUTHOR]

The current study aimed at exploring the relationship between objective disability, illness perceptions, resilience, fear of COVID-19, and psychological distress (i.e., anxiety, depression, and stress) in people with multiple sclerosis (pwMS) during the second wave of the COVID-19 outbreak. A group of 122 pwMS recruited in an Italian university hospital took part in this cross-sectional monocentric study. Hierarchical multiple linear regression analyses were performed to assess the strength of the hypothesized associations. Results indicated that, differently from cognitive impairment, motor disability was positively associated with anxiety. However, accounting for subjective illness perception, such association was no longer significant. Moreover, accounting for both protective and risk factors in the models, even illness perception was no longer significant, highlighting the central role of resilience and fear of COVID-19 in explaining the negative emotional outcomes. Implications for clinical interventions and psychoeducational trainings are discussed. [ABSTRACT FROM AUTHOR]

This study investigates longitudinal changes in health-related quality of life (HRQoL) in early-onset multiple sclerosis (MS) patients and explores the impact of disease activity (relapses) on such changes. People with MS (PwMS) onset between 12 and 25 years of age were followed longitudinally. At baseline and at year 4, patients were asked to fill the Paediatric Quality of life inventory (PedsQL). Demographic and clinical features were collected at both time points. Longitudinal within-group comparison of HRQoL total score and sub-scores was performed via paired samples t-test. The effect of relapses on the HRQoL changes over time was explored via linear mixed-effects analysis. No longitudinal changes were observed in the overall PedsQL score, nor in the physical, school and psychological functioning. An increase in the social functioning subscale (p < 0.001) and a decrease in the emotional subscale (p = 0.006) were observed. The change in social functioning, but not the one in the emotional subscale, was affected by the occurrence of relapses (p = 0.044). In conclusion, stimulating the patients to accept their emotional responses to health-related limitations, while preserving their social and relational resources seems key to the preservation of an adequate QoL over time in juvenile-onset MS. [ABSTRACT FROM AUTHOR]

Introduction: Online sexual activities (OSAs) are sexual behaviors involving online sexual content and stimuli and are categorized into non-arousal (N-OSAs), solitary arousal (S-OSAs), and partnered arousal activities (P-OSAs). As such activities in older age remain largely underexamined, this study aimed to explore OSAs in a sample of Italian older adults, analyzing their associations with gender and sexual orientation, considering sexual permissiveness as a moderator. Methods: One hundred and fourteen cisgender participants (85 men and 29 women) aged from 52 to 79 years old (M = 62.57, SD = 6.19) were recruited between September 2019 and January 2020 to participate in an online cross-sectional survey on OSAs. Results: A total of 58.1% of participants had engaged in S-OSAs at least 2–3 times during the previous month, while a lower percentage of participants had engaged in N-OSAs (38.6%) and P-OSAs (29.9%) at least 2–3 times during the previous month. Men were more likely to be engaged in S-OSAs than women. Being non-heterosexual was associated with an increase in engagement in N-OSAs and S-OSAs, but not in P-OSAs. Sexual permissiveness was significantly associated with N-OSAs and S-OSAs, but not with P-OSAs, and did not moderate either the relationship of gender with OSAs or that of sexual orientation with OSAs. Conclusions: This study sheds light on the need to include online resources in sexual health educational programs addressed at older people, as well as in training programs addressed at healthcare professionals and social workers working with this population. [ABSTRACT FROM AUTHOR]

Background: People with multiple sclerosis (MS) may experience sexual dysfunction throughout the disease course. Validated scales to assess sexual dysfunction in MS for Italian patients are lacking. Hence, we aimed at validating Multiple Sclerosis Intimacy and Sexuality Questionnaire (MSISQ-19) for Italian MS patients. Methods: We included both male and female MS patients. Each patient completed the Italian translation of the MSISQ-19. Construct validity was explored by the exploratory factor analysis and the Cronbach's alpha coefficient. Test-retest stability and concurrent internal and external validity were examined by Pearson' correlation coefficients. Results: We enrolled 369 MS patients (323 female and 46 male). Italian MSISQ-19 showed a Cronbach's alpha of 0.92. MSISQ-19 test and retest total scores correlated between each other (r = 0.48, p = 0.01). MSISQ-19 total score also correlated with primary, secondary and tertiary subscales (p < 0.001). Conclusion: The Italian Version of the MSISQ-19 showed satisfactory internal consistency and reliability with moderately adequate test-retest reproducibility, suggesting that it may be used as a valuable measure of sexual dysfunction in the Italian population. [ABSTRACT FROM AUTHOR]

Aim: The Brief Repeatable Battery of Neuropsychological Tests (BRB) is frequently used to estimate cognitive function in adults with multiple sclerosis (MS), while it has been included in few studies on young MS, also because of the absence of normative values. We aim to evaluate the impact of age, gender, and education on BRB scores in a young adolescent population.Methods: We administered the BRB to 76, 14-to-17-year-old, healthy subjects. Linear regression models were used to assess the impact of age, gender, and education on sub-test scores. When statistically significant (p < 0.05), we used the regression coefficient to correct the raw scores.Results: Younger age was associated with better performance on SPART (β = - 2.54; p < 0.05) and SPART-D (β = - 1.06; p < 0.05). Male gender was associated with better performance on SPART (β = 3.40; p < 0.05), SPART-D (β = 1.41; p < 0.05), PASAT-3 (β = 5.58; p < 0.05), and PASAT-2 (β = 5.07; p < 0.05). Educational attainments were associated with better performance on SPART (β = 3.23; p < 0.05) and SPART-D (β = 1.28; p < 0.05). Cut-off points were suggested at the 5th lowest percentile.Interpretation: Age, gender, and education must be accounted for when applying the BRB to young population. Present results can prove useful for future clinical and research applications in adolescent MS patients. [ABSTRACT FROM AUTHOR]

The Paris Agreement is the last hope to keep global temperature rise below 2°C. The consensus agrees to holding the increase in global average temperature to well below 2°C above pre-industrial levels, and to aim for 1.5°C. Each Party’s successive nationally determined contribution (NDC) will represent a progression beyond the party’s then current NDC, and reflect its highest possible ambition. Using Ireland as a test case, we show that increased mitigation ambition is required to meet the Paris Agreement goals in contrast to current EU policy goals of an 80-95% reduction by 2050. For the 1.5°C consistent carbon budgets, the technically feasible scenarios' abatement costs rise to greater than €8,100/tCO2 by 2050. The greatest economic impact is in the short term. Annual GDP growth rates in the period to 2020 reduce from 4% to 2.2% in the 1.5°C scenario. While aiming for net zero emissions beyond 2050, investment decisions in the next 5-10 years are critical to prevent carbon lock-in. Key policy insights Economic growth can be maintained in Ireland while rapidly decarbonizing the energy system. The social cost of carbon needs to be included as standard in valuation of infrastructure investment planning, both by government finance departments and private investors. Technological feasibility is not the limiting factor in achieving rapid deep decarbonization. Immediate increased decarbonization ambition over the next 3-5 years is critical to achieve the Paris Agreement goals, acknowledging the current 80-95% reduction target is not consistent with temperature goals of ‘well below’ 2°C and pursuing 1.5°C. Applying carbon budgets to the energy system results in non-linear CO2 emissions reductions over time, which contrast with current EU policy targets, and the implied optimal climate policy and mitigation investment strategy. [ABSTRACT FROM AUTHOR]

The use of scenarios and quantitative modelling to identify pathways for energy system transformations in line with the Paris targets is well established in the field of energy and climate policy. The resulting decarbonization pathways depend on both assumptions and the type of model used (e.g., integrated assessment models, energy system, macro-econometric or bottom-up sector models). The objective of this article is to analyze how energy demand sectors in system-wide net-zero scenarios for the EU compare to the results of sector-specific models. To this end, a novel approach referred to as "sectoral benchmarking" is developed and applied, combining the application of standard indicators such as energy intensity, electrification rate or carbon intensity with an index decomposition analysis. The combined approach allows visualizing how system-wide decarbonization pathways differ from the sector models' pathways by bringing the model output in a harmonized format for an efficient comparison. The analysis compares pathways from four different modelling tools: two European system models, one of which is an energy system model (EU TIMES) and the other a macro-econometric model (NEMESIS); as well as two sector-specific models, for transport (ALADIN) and for the industry and building sectors (FORECAST). We evaluate the system model's net-zero scenarios by comparing them to a corridor given by the sector models' current policy and net-zero emission scenarios. This corridor represents what the sector models deem as plausible from their bottom-up perspective within the boundaries of current policies and ambitions to reach net-zero. Our results show that the system model net-zero pathways differ substantially from the sectoral perspective in all sectors. In the industry and building sectors, both system models' decarbonization ambitions are within the sector corridor, but the employed mitigation levers differ. In the industry sectors, the sectoral model achieves substantial CO 2 emission reductions with electrification, while the system models use more bioenergy (EU TIMES) or more energy efficiency (NEMESIS). In the building sector, both system models rely mostly on electrification, while the sector models relies on biomass and some district heat and electrification. In the transport sector, both system models' decarbonization ambition is substantially lower than the sector model's. The observed differences are caused by a variety of factors, which we evaluate in this article. One reason is the system models' lower ambition to decarbonize the end-use sectors due to their ability to compensate with negative emission technologies across sectors. In addition, employed mitigation levers differ due to the models' differing capabilities to consider technologies as well as differences in the allocation of bioenergy to sectors. Our findings can be used to determine how the different types of models can inform each other and to make the diverging decarbonization pathways more transparent to policy-makers and other relevant stakeholders. • System-wide EU net-zero scenarios are compared to sector model decarbonization. • System-wide scenarios use negative emissions instead of ambitious end-use levers. • A novel approach to assess sectoral dynamics in system-wide scenarios is presented. • Transport sector model more ambitious than system-wide net zero scenarios. • System-wide scenarios mostly within sector model range for industry and buildings. [ABSTRACT FROM AUTHOR]

The European power system is currently experiencing new challenges due to growing shares of intermittent renewable energy sources. Compared to other flexibility options, power-to-heat (PtH) technologies have a high level of maturity. The goal is, therefore, to analyse the potential for PtH in the European energy system and possible business models that enable the exploitation of these potentials. A literature review on the European and country-level potential for PtH is carried out revealing a wide range of these potentials. An own approach assesses the potential in district heating for selected European countries. PtH potential makes up a remarkable share of the peak electrical load, but varies due to the inhomogeneous nature of the investigated national energy systems and different weather conditions. [ABSTRACT FROM AUTHOR]

Purpose: To investigate the moderating role of resilience in the relationship between affective disorders and Health-Related Quality of Life (HRQoL) for adolescents and young adults with multiple sclerosis (MS).Methods: A quantitative methodology was adopted. Fifty-three adolescents and young adults were interviewed to assess resilience as a personality trait (Ego-Resiliency Scale) and resilience as an interactive competence (CYRM-28), Health-Related Quality of Life (PedsQL 4.0), depression and anxiety (BDI-II and STAI-Y).Results: Affective disorders, both depression (β = -.38, p < .001) and anxiety (State β = -.35, p < .001; Trait β = -.41, p < .001), were negatively associated with HRQoL. Data also showed that the resilience competencies using Individual (β = .22, p < .001) and relational resources (β = .12, p < .05) are significantly associated HRQoL. According to the regression analyses, we tested the moderating role of resilience competence using individual resources on the relationship between the Depression Cognitive Factor and Emotional Functioning. Data show that in step 2 of the regression analysis, we obtained a variation of β = -.45 (p < .001) to β = -.30 (p < .001) in the dimension for the Depression Cognitive Factor. The Sobel test showed that the moderating effect of resilience was significant regarding the increase in R2 (p < .01).Conclusions: Resilience competence using individual resources moderates the relationship between the Depression Cognitive Factor and Emotional Functioning in adolescents with MS. Our study suggests that to improve well-being for adolescents with MS resilience could play a key role. [ABSTRACT FROM AUTHOR]

Energy security is an interdisciplinary concept. Its definitions leaves it vulnerable to exploitation as a justification for energy policy instruments. The extensive literature has yet to converge to formulate a unified definition of energy security and many outline a multitude of methods of measurement, such as the Supply/Demand index applied here in modified form, but also the Hirshman-Herfindahl Index, the Shannon Index for fuel diversity, and others. However, many of these index methods do not explicitly take into account the techno-economic interdependencies which influence energy security within the energy system. This paper reviews the literature to establish an energy security assessment method considering perspectives from sovereignty, infrastructural robustness, and market resilience. The aim of the paper is to provide a transparent data rich method and assessment of energy security by means of an application of a systemic energy security index to historical data and potential projected techno-economic energy system scenario analysis for a decarbonising Irish Energy System. [ABSTRACT FROM AUTHOR]

The greenhouse gases (GHG) emissions from land-use change are of particular concern for land-based biofuels. Emissions avoided by substituting fossil fuels with biofuels may be offset by emissions from direct and indirect land-use changes (LUC). There is an urgent need to investigate what impact land-use change emissions may have on the expansion of bioenergy and biofuels, in the context of EU mitigation policies. This paper focuses on Ireland, which faces a number of challenges in delivering its renewable energy and GHG reduction targets. The Irish TIMES energy systems model was used to assess the impact of a range of land-use change emissions' levels on the evolution of Ireland's low-carbon energy system. A reference scenario was developed where LUC is ignored and Ireland achieves a least-cost low-carbon energy system by 2050. If high indirect land-use change (ILUC) emissions are included, this results in a decrease by 30 % in bioenergy and a 68 % increase in marginal abatement costs by 2050. Hydrogen is used instead of bioenergy in the freight sector in this scenario, while private cars are fuelled by renewable electricity. If GHG emissions from ILUC were considered less severe, indigenous grass biomethane becomes the key biofuel representing 31 % of total bioenergy consumption. This is in line with recent research in Ireland of the key role that grass biomethane can play. [ABSTRACT FROM AUTHOR]

Agriculture is responsible for approximately 25% of anthropogenic global GHG emissions. This significant share highlights the fundamental importance of the agricultural sector in the global GHG emissions reduction challenge. This article develops and tests a methodology for the integration of agricultural and energy systems modelling. The goal of the research is to extend an energy systems modelling approach to agriculture in order to provide richer insights into the dynamics and interactions between the two (e.g. in competition for land-use). We build Agri-TIMES, an agricultural systems module using the TIMES energy systems modelling framework, to model the effect of livestock emissions and explore emissions reduction options. The research focuses on Ireland, which is an interesting test case for two reasons: first, agriculture currently accounts for about 30% of Ireland's GHG emissions, significantly higher than other industrialized countries yet comparable with global levels (here including emissions associated with other land-use change and forestation); second, Ireland is both a complete and reasonably sized agricultural system to act as a test case for this new approach. This article describes the methodology used, the data requirements, and technical assumptions made to facilitate the modelling. It also presents results to illustrate the approach and provide associated initial insights. Policy relevance Most of the policy focus with regard to climate mitigation targets has been on reducing energy-related CO2emissions, which is understandable as they represent by far the largest source of emissions. Non-energy-related GHG emissions – largely from agriculture, industrial processes, and waste – have received significantly less attention in policy discourse. Going forward, however, if significant cuts are made in energy-related CO2emissions, the role of non-energy-related GHG emissions will grow in importance. It is therefore crucial that climate mitigation analyses and strategies are not limited to the energy system. This article shows the value of using integrated energy and agriculture techno-economic modelling techniques to draw evidence for new comprehensive climate policy strategies able to discern between the full range of technical solutions available. It enables the production of economy-wide least-cost climate mitigation pathways. [ABSTRACT FROM AUTHOR]

The published version of this article unfortunately contained a mistake in Table 2. [ABSTRACT FROM AUTHOR]

Physical disability impacts psychosocial wellbeing in people with multiple sclerosis. However, the role of physical activity in this context is still debated. By taking advantage of a previous survey, conducted online from 22 April to 7 May 2020, we performed a post-hoc analysis with the aim to assess the associations between disability, physical exercise, and mental health in multiple sclerosis. We retrieved the following data: (i) sociodemographic information, (ii) changes in lifestyle (including exercise), (iii) physical disability, as measured with the Patient-Determined Disease Steps scale, and (iv) anxiety feelings and depressive symptoms assessed via the items included in the Quality of Life in Neurological Disorders measurement system. Examination of the interaction plot showed that the effect of disability on depression, but not on anxious symptoms, was significant for all levels of physical exercise (low: b = 1.22, 95% C.I. 0.85, 1.58, p < 0.001; moderate: b = 0.95, 95% C.I. 0.66, 1.24, p < 0.001; and high: b = 0.68, 95% C.I. 0.24, 1.13, p = 0.003). Based on these data, we can conclude that disability significantly impacted depression during the COVID-19 pandemic, with physical activity playing a moderating role. Our results suggest that favoring exercise in multiple sclerosis (MS) would ameliorate psychological wellbeing regardless of the level of physical disability. [ABSTRACT FROM AUTHOR]

The recent outbreak of coronavirus disease 2019 (COVID-19) has been extremely stressful and has produced fear and anxiety throughout the population, representing a psychological emergency. This work aimed at presenting a mental health first aid service established within an Italian university public hospital context to address four different population targets (i.e., people vulnerable to mental health problems, health-care professionals, people in isolation, and general citizenship). Specifically, the organizational structure comprising four different areas (i.e., management, clinical, communication, and research) and first data collected from the foundation of the service until 3 May 2020 are presented. Findings indicated that anxiety and fear of contagion were the main motivations prompting both the general population and health-care professionals to ask for a psychological help. Furthermore, findings indicate that clients' current quality of life was perceived as lower than in the past but also that imagined in the future, highlighting the importance of psychological first aid interventions. This service may represent an example for helping mental health professionals in developing similar services in their local realities, promoting health and individual and community resilience. [ABSTRACT FROM AUTHOR]