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The spontaneous exchange bias effect (SEB) has wide application prospects in information storage technologies. In this study, nanoscale raw materials were used to fabricate antiferromagnetic Fe3BO6 polycrystals. The obtained Fe3BO6 exhibited a large SEB effect, where the value of the spontaneous exchange bias field at room temperature was as large as ∼4234 Oe. The room-temperature training effect, temperature-dependence, and maximum field-dependence of the HSEB were investigated. We propose that this giant SEB originates from the exchange-coupling interactions between the weak ferromagnetic surface state and the bulk antiferromagnetic state. The theoretical analysis results were further verified by comparing the magnetic properties of the Fe3BO6 with relatively low crystallinity. The results of this investigation will help find promising candidate materials for devices based on the SEB effect. [ABSTRACT FROM AUTHOR]

Solid-state refrigeration using barocaloric materials is environmentally friendly and highly efficient, making it a subject of global interest over the past decade. Here, we report giant barocaloric effects in sodium hexafluorophosphate (NaPF6) and sodium hexafluoroarsenate (NaAsF6) that both undergo a cubic-to-rhombohedral phase transition near room temperature. We have determined that the low-temperature phase structure of NaPF6 is a rhombohedral structure with space group R 3 ¯ by neutron powder diffraction. There are three Raman active vibration modes in NaPF6 and NaAsF6, i.e., F2g, Eg, and A1g. The phase transition temperature varies with pressure at a rate of dTt/dP = 250 and 310 K GPa−1 for NaPF6 and NaAsF6. The pressure-induced entropy changes of NaPF6 and NaAsF6 are determined to be around 45.2 and 35.6 J kg−1 K−1, respectively. The saturation driving pressure is about 40 MPa. The pressure-dependent neutron powder diffraction suggests that the barocaloric effects are related to the pressure-induced cubic-to-rhombohedral phase transitions. [ABSTRACT FROM AUTHOR]

Owing to the unique asymmetric geometry, Janus monolayer compounds exhibit various exotic thermal properties and have promising applications in thermal management. In this study, we combine machine learning potentials and the phonon Boltzmann transport equation to perform a comparative study of the thermal transport properties in Janus XBAlY (X = Se, S, Te; Y = S, Se, O; X ≠ Y) monolayers. Our findings unveil a thermal conductivity (κ p) ranking as SeBAlS > TeBAlO > SBAlSe, contradicting the conventional expectation that a higher κ p is typically observed when the average atomic mass is smaller. At room temperature, the κ p of SeBAlS is 174 Wm−1 K−1, which is 4.8 times that of SBAlSe when considering three-phonon scattering processes. Moreover, the consideration of four-phonon scatterings does not alter such ranking. The anomalous κ p phenomenon was explained through a detailed analysis of the phonon–phonon scattering mechanism, phonon bandgap, phonon anharmonicity, and chemical bond strength. This study highlights the intricate relationship between atomic mass, bonding characteristics, and thermal properties, offering insights for designing Janus materials with tailored thermal conductivity. [ABSTRACT FROM AUTHOR]

Carbon-based Materials have been extensively researched for their prospect in the fields of environment and energy, especially for graphene oxide (GO). In this work, a novel sodium dodecyl sulfate (SDS)-assisted synthesis of BiOBr/Bi2WO6/GO ternary composite has been synthesized successfully by a handy hydrothermal method. Photoluminescence, Photocurrent, Electrochemical Impedance Spectroscopy, surface photovoltage and transient photovoltage measurements illustrate that construction of p-n BiOBr/Bi2WO6 heterojunction leads to the obviously enhancement of charge separation efficiency, and the photogenerated electrons trapped by GO can effectively inhibit the recombination process of photogenerated charge, resulting in the improvement of charge separation efficiency and the longer lifetime of photogenerated carriers for BiOBr/Bi2WO6/GO. The characterization of structure and morphology indicate that role of GO can also improve the visible light absorption range, and the SDS-assisted synthesis can reduce the size of particle in the composite and enhances the specific surface area of the composite by regulating the particle size and agglomeration. Under optimal conditions, BiOBr/Bi2WO6/GO (SDS) has the outstanding photocatalytic degradation performance and the degradation rate constants for oxytetracycline, tetracycline hydrochloride, methylene blue and rhodamine are 0.056, 0.057, 0.103 and 0.414 min−1, respectively. Notably, the degradation rate constants obtained by BiOBr/Bi2WO6/GO (SDS) are more ten times higher than that of pure BiOBr and Bi2WO6. The possible mechanism of photocatalytic degradation was suggested for BiOBr/Bi2WO6/GO based on the dynamic properties of photogenerated charge and reactive oxidation species results. Surprisingly, the recyclability of the BiOBr/Bi2WO6/GO (SDS) composite obtained from the cyclic experiments has laid a foundation for the study of efficient and stable photocatalysts. [ABSTRACT FROM AUTHOR]

Computational toxicology provides the basis for the accurate assessment and prediction of toxic effects at the molecular level. However, classical computing often lacks speed and accuracy for complex chemical systems, leading to bottlenecks. The emergence of quantum computing has the potential to fundamentally break through these constraints, allowing the exploration of toxic effects from molecular initiation events (MIEs). This review prospects the application of quantum computing in environmental science. It provides a brief overview of classical computational chemistry and quantum computing, elucidating the quantitative mapping between these two approaches. Cytochrome P450 (CYP) enzymes, an important biomarker for monitoring and identifying environmental risks of pollutants, is taken as an example to summarize the progress of classical algorithms in explaining its metabolic mechanism. By comparing the required runtimes of quantum and classical computing approaches, the review highlights the potential quantum advantage in high-precision chemical simulations, especially in large-scale CYP model calculations. A hybrid quantum-classical strategy is specially developed for the practical workflow of quantum computing in environmental science. By means of the close collaboration between theory and experiment, it shows great potential in deeply understanding the environmental impact of emerging pollutants and designing relevant environmental monitoring programs. This review can provide valuable clues for future sustainable solutions. Through the effective synergy between quantum computing development and precision management of environmental pollution, it is expected to revolutionize the way we understand and mitigate the impacts of emerging pollutants. [ABSTRACT FROM AUTHOR]

Due to the exceptional flame retardancy, environmental protection, cost‐effectiveness, and ease of processing, halogen‐free flame‐retardant polyethylene (PE) composites are attracting wide attention. However, the addition of halogen‐free flame retardants usually leads to significantly decreased mechanical properties of PE composites, which greatly limits its application. Herein, we report a halogen‐free flame‐retardant PE/ethylene‐vinyl acetate copolymer composite (PE/EVA) with excellent flame retardancy and mechanical properties by combining compatibilizer, crosslinking agent, and carbon fiber. The presence of compatibilizer not only improves the compatibility of halogen‐free flame retardants and matrix, but also interacts with the crosslinking agent to form a cladding structure on the surface of carbon fiber. The enhanced interaction between carbon fiber surface and matrix then results in significantly enhanced flame retardancy and mechanical properties of the composite. Specifically, adding 4 phr compatibilizer, 0.5 phr crosslinking agent, and 10 phr carbon fiber will lead to the halogen‐free flame‐retardant PE/EVA composite with a UL‐94 V‐1 level, limiting oxygen index of 27.1, and an increased tensile strength by 84.02% reaching 19.12 MPa. Moreover, further carbon fiber surface modification can further enhance the flame retardancy of the composite probably due to a synergistic flame‐retardant effect caused by the surface modified nitrogen and silicon elements. As a result, the composite achieves UL‐94 V‐0 level and the limiting oxygen index of 27.7 with well‐retained high tensile strength of 15.19 MPa. This work provides a practical strategy for enhancing the flame retardancy and mechanical property of PE‐based composite simultaneously. Highlights: Compatibilizer interacts with crosslinking agent to form a cladding structure on the surface of carbon fiber.Crosslinking agent promotes the formation of chemical bonds between modified carbon fiber, compatibilizer, and matrix.Carbon fiber surface modification can enhance the flame retardancy of the composite due to the synergistic flame‐retardant effect. [ABSTRACT FROM AUTHOR]

CD8+ T cells play a critical role in cancer immune‐surveillance and pathogen elimination. However, their effector function can be severely impaired by inhibitory receptors such as programmed death‐1 (PD‐1) and T cell immunoglobulin domain and mucin domain‐3 (Tim‐3). Here Siglec‐G is identified as a coinhibitory receptor that limits CD8+ T cell function. Siglec‐G is highly expressed on tumor‐infiltrating T cells and is enriched in the exhausted T cell subset. Ablation of Siglec‐G enhances the efficacy of adoptively transferred T cells and chimeric antigen receptor (CAR) T cells in suppressing solid tumors growth. Mechanistically, sialoglycan ligands, such as CD24 on tumor cells, activate the Siglec‐G‐SHP2 axis in CD8+ T cells, impairing metabolic reprogramming from oxidative phosphorylation to glycolysis, which dampens cytotoxic T lymphocyte (CTL) activation, expansion, and cytotoxicity. These findings discover a critical role for Siglec‐G in inhibiting CD8+ T cell responses, suggesting its potential therapeutic effect in adoptive T cell therapy and tumor immunotherapy. [ABSTRACT FROM AUTHOR]

Following the relaxation of control measures for COVID-19 in China in December 2022, there was a resurgence of influenza A among children in Linyi. This research aims to analyze the epidemiology and genetic characteristics of influenza A viruses circulating among children in Linyi from April 2022 to March 2024. Throat swab specimens were collected from children with influenza-like illness (ILI), and the types of influenza were identified. The hemagglutinin (HA) genes of influenza A viruses were amplified using reverse transcription-polymerase chain reaction (RT-PCR) and then sequenced. Subsequently, the gene characteristics and phylogenetics of these sequences were analyzed. A total of 6258 cases of influenza A were tested from 54,926 children with ILI over two years, showing three epidemic waves: one in summer 2022 and two in spring and winter 2023. The epidemic waves in summer 2022 and winter 2023 were caused by influenza A(H3N2) viruses. In spring 2023, influenza A(H1N1)pdm09 viruses accounted for 77.1% and A(H3N2) viruses accounted for 22.9%, respectively. The influenza A(H1N1)pdm09 viruses belonged to clade 6B.1A.5a.2a, while the influenza A(H3N2) viruses evolved from clade 3C.2a1b.2a.1a to 3C.2a1b.2a.2a.3a.1. Compared with the vaccine strain of the Northern Hemisphere of the current year, multiple amino acid substitutions and glycosylation sites changes were observed in the HA protein that most likely resulted in antigenic drift. The influenza A viruses have been circulating and evolving persistently in Linyi from 2022 to 2024. Continuous surveillance is essential for comprehending the dynamics of influenza in the post-COVID-19 era. [ABSTRACT FROM AUTHOR]

Background: To investigate the accuracy of quantitative blood oxygen level-dependent (qBOLD) magnetic resonance imaging (MRI) in identifying hypoxia within glioblastoma and explore dynamic changes in oxygenation status of glioblastoma with and without metformin administration. Methods: Three healthy and seven C6-bearing rats underwent 7-T qBOLD MRI. Oxygen extraction fraction (OEF) and cerebral metabolism rate of O2 (CMRO2) were calculated from qBOLD data. Tumor tissues were stained using hypoxia-inducible factor-1 α (HIF-1 α ) and pimonidazole. The correlation between the hypoxia markers and corresponding qBOLD-based parameters was analyzed. Six C6-bearing rats were divided into metformin-treated and control groups for a longitudinal study of qBOLD imaging changes, with scans conducted on the 12th, 15th, and 18th day post-tumor implantation. Results: In healthy rats, gray matter showed higher values than white matter in T2, T2*, cerebral blood volume (CBV), and cerebral blood flow (CBF), whereas OEF was lower. Glioblastoma tissues exhibited elevated T2, T2*, CBV, and CBF but decreased OEF and CMRO2 relative to normal-appearing white matter. No significant correlation was found between staining scores from HIF-1 α and pimonidazole. T2* and T2 values were negatively correlated with pimonidazole scores in tumor regions. As the tumor progressed, OEF values increased with intra-tissue variations, whereas CMRO2 decreased. Metformin delayed the reduction of T2 and T2* values, with significant differences in OEF and CMRO2 values compared to controls on day 18. Conclusion: T2* and T2 values were significantly associated with the hypoxia status in glioma. Metformin could potentially mitigate the progression of hypoxia in glioblastoma, which can be tracked by qBOLD parameters. Relevance statement: This study demonstrates the potential of qBOLD parameters in assessing glioma dynamic oxygen metabolism and the efficacy of metformin as an anti-hypoxic agent, providing insights into improving glioblastoma treatment strategies. Key Points: The study investigated qBOLD imaging's accuracy in identifying hypoxia status within glioblastoma. qBOLD effectively assesses hypoxia and its dynamic evolution in glioblastoma. qBOLD parameters assist in identifying a suitable patient demographic for metformin treatment. [ABSTRACT FROM AUTHOR]

Variable-frequency air injection and extraction is critical for salt cavern compressed air energy storage (CAES) power plants to smoothly integrate electricity into the power grid, and the magnitude of its effect on the mechanical properties and damage progression of the salt cavern surrounding rock determines the operation scheme of CAES power plants. To examine the impact of variable frequency, a series of monotonic loading tests with different rates and variable-frequency fatigue (VFF) tests with different upper limit stresses were carried out. Dilatancy stresses have a positive power function relationship with loading rate and frequency, but the fatigue loading process weakens this nonlinear tendency. The rate of change (CR) or the exponent of the fitted function can express it. From the initial stage to the steady stage, the CR of dilatancy stress increases about three times, and the CR of residual volumetric strain decreases about five to eight times. Increased upper-limit stress weakens this trend of change. Both high speed and high-frequency loading shift the damage acceleration point forward (e.g., b value accelerates earlier). The damage rate correlation is significant in the later stages of the VFF tests, the rate correlation of damage is significant. Both the upper stress and the extension of the main fracture zone enhance the rate dependence of fracture types. Moreover, the effect of rate on crystal penetrating cracks is more pronounced in the high-stress interval. The number of fractures significantly affects the dilatancy, rather than the type of fracture. This study serves as a guide for the design of variable frequency programs in different CAES salt cavern operation stages, as well as subsequent research. Highlights: The variable-frequency test, avoiding rock dispersion, is more reliable and more in line with engineering than fixed-frequency and fixed-speed tests. Crack amount, not type, decides dilatancy. Stress affects the high frequency-high amplitude signal. Fast speed and high-frequency advance damage. Dilatancy rate correlation is explained by the dislocation slip, crack extension type and the stress concentration. We propose some CAES power plant frequency conversion ideas based on the correlation between crack and dilatancy rates. [ABSTRACT FROM AUTHOR]

Objective: To report a case of bilateral diffuse uveal melanocytic proliferation (BDUMP) followed by massive unilateral uveal proliferation. Methods: Retrospective case report. Results: A 47-year-old female with history of metastatic ovarian carcinoma initially presented with bilateral vision loss and multifocal red patches on posterior poles consistent with BDUMP. Five years later, she presented with bilateral neovascular glaucoma and unilateral iris and ciliary body mass concerning for malignancy. Enucleation revealed diffuse uveal growth involving almost the entirety of the uveal tract. Conclusions: BDUMP can rarely be associated with uveal proliferation. Routine examinations are recommended to monitor for any changes concerning malignancy. [ABSTRACT FROM AUTHOR]

Background: To clarify the psychological experience and coping strategies in patients with acute pancreatitis in pregnancy (APIP) and propose interventional measures to improve pregnancy outcomes in these women. With an increasing trend of pregnant women in advanced ages and multiparous women, the incidence of APIP has significantly increased. Pregnancy accompanied by concurrent pancreatitis may subject these women to notable psychological stress, which is a factor that has been infrequently reported in previous studies. Methods: APIP patients were interviewed from December 2020 to June 2021. Data were collected through semi-structured interviews based on an outline, including six questions. The interviews were recorded and analyzed using qualitative content analysis until data saturation was reached. Results: Ten APIP patients were interviewed and four themes were identified, including excessive psychological burden, uncomfortable experience, urgent requirement for adequate medical resources, and importance of social support. Conclusion: Patients with APIP suffer from significant psychological stress due to their medical conditions and management. They desired adequate medical resources and social support. The local health department, hospital administrators, and medical staff should understand the psychological requirements and provide adequate healthcare and education that are easily accessible to these APIP patients. In addition, family support should also be encouraged to promote APIP patients' recovery. [ABSTRACT FROM AUTHOR]

Cerebral ischemia-reperfusion injury produces excessive reactive oxygen and nitrogen species, including superoxide, nitric oxide, and peroxynitrite (ONOO-). We recently developed a new ONOO--triggered metal-free carbon monoxide donor (PCOD585), exhibiting a notable neuroprotective outcome on the rat middle cerebral artery occlusion model and rendering an exciting intervention opportunity toward ischemia-induced brain injuries. However, its therapeutic mechanism still needs to be addressed. In the pharmacological study, we found PCOD585 inhibited neuronal Bcl2/Bax/caspase-3 apoptosis pathway in the peri-infarcted area of stroke by scavenging ONOO-. ONOO- scavenging further led to decreased Acyl-CoA synthetase long-chain family member 4 and increased glutathione peroxidase 4, to minimize lipoperoxidation. Additionally, the carbon monoxide release upon the ONOO- reaction with PCOD585 further inhibited the neuronal Iron-dependent ferroptosis associated with ischemia-reperfusion. Such a synergistic neuroprotective mechanism of PCOD585 yields as potent a neuroprotective effect as Edaravone. Additionally, PCOD585 penetrates the blood-brain barrier and reduces the degradation of zonula occludens-1 by inhibiting matrix metalloproteinase-9, thereby protecting the integrity of the blood-brain barrier. Our study provides a new perspective for developing multi-functional compounds to treat ischemic stroke. [ABSTRACT FROM AUTHOR]

Myocardial infarction (MI) is a lethal cardiovascular disease worldwide. Emerging evidence has revealed the critical role of gut dysbiosis and impaired gut-brain axis in the pathological progression of MI. Tanshinone IIA (Tan IIA), a traditional Chinese medicine, has been demonstrated to exert therapeutic effects for MI. However, the effects of Tan IIA on gut-brain communication and its potential mechanisms post-MI are still unclear. In this study, we initially found that Tan IIA significantly reduced myocardial inflammation, apoptosis and fibrosis, therefore alleviating hypertrophy and improving cardiac function following MI, suggesting the cardioprotective effect of Tan IIA against MI. Additionally, we observed that Tan IIA improved the gut microbiota as evidenced by changing the α-diversity and β-diversity, and reduced histopathological impairments by decreasing inflammation and permeability in the intestinal tissues, indicating the substantial improvement of Tan IIA in gut function post-MI. Lastly, Tan IIA notably reduced lipopolysaccharides (LPS) level in serum, inflammation responses in paraventricular nucleus (PVN) and sympathetic hyperexcitability following MI, suggesting that restoration of Tan IIA on MI-induced brain alterations. Collectively, these results indicated that the cardioprotective effects of Tan IIA against MI might be associated with improvement in gut-brain axis, and LPS might be the critical factor linking gut and brain. Mechanically, Tan IIA-induced decreased intestinal damage reduced LPS release into serum, and reduced serum LPS contributes to decreased neuroinflammation with PVN and sympathetic inactivation, therefore protecting the myocardium against MI-induced injury. [ABSTRACT FROM AUTHOR]

Accurate detection and analysis of bubble size and shape in bubbly flow are critical to understanding mass and heat transfer processes. Convolutional neural networks have limitations in different bubble images due to their dependence on large amounts of labeled data. A new foundational Segment Anything Model (SAM) recently attracts lots of attention for its zero‐shot segmentation performance. Herein, we developed a novel image processing method named bubSAM, which achieves efficient and accurate bubble segmentation and shape reconstruction based on SAM. The segmentation performance of bubSAM is 30% higher than that of SAM, and its accuracy reaches 90% under different bubbly flow conditions. The accuracy of bubble shape reconstruction (BSR) algorithm in bubSAM is about 30% higher than that of typical ellipse fitting method, thus better restoring the geometric shape of bubbles. BubSAM can provide great support for understanding gas–liquid multiphase flow and design of industrial multiphase reactors. [ABSTRACT FROM AUTHOR]

The urgent need for integrated molding and sintering across various industries has inspired the development of additive manufacturing (AM) ceramics. Among the different AM technologies, direct laser additive manufacturing (DLAM) stands out as a group of highly promising technology for flexibly manufacturing ceramics without molds and adhesives in a single step. Over the last decade, significant and encouraging progress has been accomplished in DLAM of high-performance ceramics, including Al2O3, ZrO2, Al2O3/ZrO2, SiC, and others. However, high-performance ceramics fabricated by DLAM face challenges such as formation of pores and cracks and resultant low mechanical properties, hindering their practical application in high-end equipment. Further improvements are necessary before they can be widely adopted. Methods such as field-assisted techniques and post-processing can be employed to address these challenges, but a more systematic review is needed. This work aims to critically review the advancements in direct selective laser sintering/melting (SLS/SLM) and laser directed energy deposition (LDED) for various ceramic material systems. Additionally, it provides an overview of the current challenges, future research opportunities, and potential applications associated with DLAM of high-performance ceramics. [ABSTRACT FROM AUTHOR]

Climate change, earthquakes, and human activities are accelerating the degradation of permafrost, leading to loess failures and slope instability. Some engineering corridors (ECs)/infrastructures located on the northeastern margin of the Qinghai-Tibet Plateau (NE-QTP) of China are heavily influenced by landslide phenomena due to being built on permafrost, loess, and seasonally frozen ground. However, few systematic investigations have been carried out in this area. To compile a comprehensive landslide inventory, we visually interpreted 11,914 landslides in GaoFen-6 images taken from 2021 to 2022. We observe that approximately 44.85% of the infrastructures are affected by landslides. Then, based on the ground types and triggering factors, landslides are classified into three types: freeze‒thaw landslides (FTLs), loess landslides (LLs), and general landslides (GLs). More specifically, FTLs are mainly distributed in the boundary regions between permafrost and seasonally frozen ground. The LLs exhibit high-density clustered distribution characteristics. GLs have significant transitional characteristics and commonalities between FTLs and LLs. Furthermore, we apply the geographical detector to determine the controlling factors of the landslides that occurred. We find that the temperature change is the primary controller on the FTLs. The water exhibits a certain correlation with LLs. And the earthquake is the most important factor on the GLs. Our study provides a significant dataset for quantifying the analysis of landslides in NE-QTP. [ABSTRACT FROM AUTHOR]

Renal fibrosis contributes to chronic renal failure and a decline in the quality of life. Bushen Huoxue (BSHX) formula is a Traditional Chinese Medicine used to treat chronic renal failure. However, its mechanisms of action remain unclear. In this study, a rat model of renal fibrosis was constructed by 5/6 nephrectomy in vivo, and histopathological changes were analyzed using hematoxylin-eosin and Masson's trichrome staining. Angiotensin II (Ang II) was used to establish an in vitro renal fibrosis cell model in vitro. Pyroptosis was measured using flow cytometry. Related markers of fibrosis and NOD-like receptor protein 3 (NLRP3) inflammasome activation were measured using western blotting and enzyme-linked immunosorbent assay. Treatment with BSHX (0.25, 0.5, and 1 g/kg) significantly inhibited renal fibrosis and damage in 5/6 nephrectomized rats and simultaneously reduced oxidative stress and NLRP3 inflammasome activation. Similarly, BSHX treatment reduced the levels of hydroxyproline, transforming growth factor-β, matrix metalloproteinase 2, and matrix metalloproteinase 9 and inactivated the Smad2/3 signaling pathway in Ang II-treated HK-2 cells. Our data also showed that treatment with BSHX reduced NLRP3 inflammasome activation and pyroptosis in Ang II-treated HK-2 cells. Moreover, fibrosis and pyroptosis in HK-2 cells induced by NLRP3 overexpression were reduced by treatment with BSHX. BSHX significantly reduced renal fibrosis and pyroptosis, and its mechanism was mainly associated with the inhibition of reactive oxygen species (ROS)/NLRP3-mediated inflammasome activation. [ABSTRACT FROM AUTHOR]

Although rotating blades are designed to operate away from structural frequencies, internal resonance can still occur at certain rotation speeds, significantly affecting the blades' stiffness. Therefore, a detailed investigation of the resonance mechanisms of rotating blades, especially those introduced by nonlinearities, is crucial for structural design and optimization. To address this, a dynamic model of a functionally graded graphene/titanium alloy composite trapezoid plate is established to investigate the nonlinear dynamics of a rotating blade with varying cross-sections. The ordinary differential equations of the plate are formulated based on the energy method and Lagrange equations. The occurrence of 1:3 internal resonance in the trapezoid plate is identified through the analysis of linear and nonlinear free vibrations. Subsequently, the steady-state responses of the rotating plate in the case of 1:3 internal resonance are numerically calculated and verified using the approximation solution of the multiple scales method. Parametric studies are conducted to investigate the effects of rotation speed, taper ratio, excitation amplitude, and excitation frequency on the amplitude–frequency and amplitude–force curves. [ABSTRACT FROM AUTHOR]

AUXIN/INDOLE‐3‐ACETIC ACIDs are transcriptional repressors for auxin signalling. Aux/IAAs of Arabidopsis thaliana display some functional redundancy. The IAA3/SHY2 clade (IAA1, IAA2, IAA3 and IAA4) show strong sequence similarity, but no higher‐order mutants have been reported. Here, through CRISPR/Cas9 genome editing, we generated loss‐of‐function iaa1/2/3/4 mutants. The quadruple mutants only exhibited a weak phenotype. Thus, we additionally knocked out IAA7/AXR2 and IAA16, which are coexpressed with IAA1/2/3/4. Remarkably, under white light control conditions, the iaa1/2/3/4/7/16 mutants exhibited a shade avoidance‐like phenotype with over‐elongated hypocotyls and petioles and hyponastic leaves. The sextuple mutants were highly sensitive to low light intensity, and the hypocotyl cells of the mutants were excessively elongated. Transcriptome profiling and qRT‐PCR analyses revealed that the sextuple mutation upregulated IAA19/MSG2 and IAA29, two shared shade/auxin signalling targets. Besides, genes encoding cell wall‐remodelling proteins and shade‐responsive transcription regulators were upregulated. Using dual‐luciferase reporter assays, we verified that IAA2/IAA7 targeted the promoters of cell wall‐remodelling genes to inhibit their transcription. Our work indicates that the IAA1/2/3/4/7/16 gene set is required for the optimal integration of auxin and shade signalling. The mutants generated here should be valuable for exploring the complex interactions among signal sensors, transcription activators and transcription repressors during hormone/environmental responses. Summary Statement: A highly conserved and coexpressed gene set of Arabidopsis thaliana Aux/IAAs was inactivated by genomic editing. The shade‐avoidance‐like phenotypes of these iaa1/2/3/4/7/16 sextuple mutants revealed their essential roles for the optimal integration between light and auxin signalling pathways. [ABSTRACT FROM AUTHOR]

Facilitated subcutaneous immunoglobulin (fSCIG) 10% is an immunoglobulin replacement therapy that utilizes recombinant human hyaluronidase (rHuPH20) to enhance immunoglobulin dispersion and absorption, allowing for longer treatment intervals similar to intravenous immunoglobulin (up to once monthly). fSCIG 10% is indicated in the USA for treating adults and children aged ≥ 2 years with primary immunodeficiency diseases (PIDs). This prospective, non-interventional, open-label, multicenter, post-authorization safety study (NCT02593188) was conducted in the USA from November 2015 to October 2021 to assess the long-term safety of fSCIG 10% in routine clinical practice. Patients with PIDs aged ≥ 16 years who were prescribed and/or had started fSCIG 10% treatment were enrolled. In total, 253 patients were enrolled and included (full analysis set). Participants received fSCIG 10% treatment for a median (interquartile range) of 10.0 (3.5–11.8) months, with the majority of infusions administered every 4 weeks (54.4% [1197/2201 infusions]) and at home (62.6% [1395/2230 infusions]). Overall, 98.5% of infusions were administered without rate reduction, interruption, or discontinuation due to adverse events (AEs). Treatment-related, non-serious AEs were experienced by 52 patients (20.6%, 284 events). Two patients (0.8%) each experienced one treatment-related serious AE (aseptic meningitis and deep vein thrombosis). Development of antibodies against rHuPH20 was uncommon; 14/196 patients (7.1%) tested positive for binding antibodies (titer ≥ 1:160) with no neutralizing antibodies detected. There was no relationship between anti-rHuPH20 antibody positivity and the occurrence of treatment-related serious or non-serious AEs. Long-term, repeated self-administration of fSCIG 10% was well tolerated in US clinical practice by patients with PIDs. [ABSTRACT FROM AUTHOR]

Neurofibromatosis type 1 (NF1) is a prevalent autosomal dominant disorder caused by mutations in the NF1 gene, leading to multisystem disorders. Given the critical role of cysteine residues in protein stability and function, we aimed to identify key NF1 mutations affecting cysteine residues that significantly contribute to neurofibromatosis pathology. To identify the most critical mutations in the NF1 gene that contribute to the pathology of neurofibromatosis, we employed a sophisticated computational pipeline specifically designed to detect significant mutations affecting the NF1 gene. Our approach involved an exhaustive search of databases such as the Human Gene Mutation Database (HGMD), UniProt, and ClinVar for information on missense mutations associated with NF1. Our search yielded a total of 204 unique cysteine missense mutations. We then employed in silico prediction tools, including PredictSNP, iStable, and Align GVGD, to assess the impact of these mutations. Among the mutations, C379R, R1000C, and C1016Y stood out due to their deleterious effects on the biophysical properties of the neurofibromin protein, significantly destabilizing its structure. These mutations were subjected to further phenotyping analysis using SNPeffect 4.0, which predicted disturbances in the protein’s chaperone binding sites and overall structural stability. Furthermore, to directly visualize the impact of these mutations on protein structure, we utilized AlphaFold3 to simulate both the wild-type and mutant NF1 structures, revealing the significant effects of the R1000C mutation on the protein’s conformation. In conclusion, the identification of these mutations can play a pivotal role in advancing the field of precision medicine and aid in the development of effective drugs for associated diseases. [ABSTRACT FROM AUTHOR]

Background: We conducted a study on radiation exposure in patients with gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) treated with [177Lu]Lu-DOTA-TATE in China for the first time, aiming to provide guidance and reference for radiation protection in this regard. A total of 30 GEP-NENs patients who received [177Lu]Lu-DOTA-TATE therapy were recruited in the study. We measured the external dose rate (EDR) values of each patient during the injection and 0–6 h post-administration period, as well as the radiation dose (RD) values to healthcare nurses and the surrounding environment. We performed a double exponential curve fitting and estimated the RD to the public from patients discharged at different times after [177Lu]Lu-DOTA-TATE therapy. Results: Among the 30 patients, 27 patients completed 4 cycles of [177Lu]Lu-DOTA-TATE treatments, the estimated RD to the public indicated that for adult family members, children above 10 years old, children aged 3–10 and coworkers of the patients, patients could begin daily contact at least 24 h, 48 h, 144 h and 192 h after injection to ensure that the total RD values after four treatments not exceed the limit. During the hospitalization of patients receiving [177Lu]Lu-DOTA-TATE, the cumulative dose received by the administering nurses and to the ward environment were both well below the national RD limits. Conclusions: This study conducted a fitting analysis of the decay pattern of EDR values in GEP-NENs patients undergoing [177Lu]Lu-DOTA-TATE therapy, in order to establish guidelines for patient discharge timing and provide recommendations for radiation protection for the general public after patient discharge. Trial registration A Study Comparing Treatment With Lutetium[177Lu] Oxodotreotide Injection to Octreotide LAR in Patients With GEP-NETs, NCT05459844. https://clinicaltrials.gov/study/NCT05459844?cond=NCT05459844&rank=1. Registered 5 July 2022. [ABSTRACT FROM AUTHOR]

The damnification of mitochondrion is often considered to be an important culprit of inflammatory bowel disease (IBD), however, there are fewer reports of mechanisms of mitochondria-mediated IBD treatment. Therefore, we first proposed to reboot mitochondrial energy metabolism to treat IBD by capturing the double-sided factor of ROS and creatine (Cr)-assisted energy adjustment. Herein, an oral Cr-modified selenium-based hyaluronic acid (HA) nanogel (HASe-Cr nanogel) was fabricated for treatment of IBD, through ROS elimination and energy metabolism upgradation. More concretely, due to IBD lesion-specific positive charge and the high expression of CD44, HASe-Cr nanogel exhibited dual targeted inflammatory bio-functions, and ROS-driven degradation properties in high-yield ROS levels in inflammation areas. As expected, multifunctional HASe-Cr nanogel could effectively ameliorate IBD-related symptoms, such as mitochondrial biological function restoration, inhibition of M1-like macrophage polarization, gut mucosal reconstruction, microbial ecological repair, etc., thus excellently treating IBD. Overall, the proposed strategy underlined that the great potentiality of HASe-Cr nanogel by restarting mitochondrial metabolic energy in colitis lesions, providing new a pavement of mitochondrion-mediated colitis treatment in clinical applications. [ABSTRACT FROM AUTHOR]

Background: The COVID-19 pandemic has had a profound impact on global mental health, particularly affecting vulnerable groups such as pregnant women. Post-traumatic stress disorder (PTSD) in this population may potentially hinder infant physical development, underscoring the necessity for specific interventions. This study seeks to explore the prevalence of PTSD among pregnant women during the pandemic and its implications for infant physical development, thereby providing evidence to mitigate the adverse effects of the pandemic on maternal and child health. Methods: A prospective cohort study was carried out involving 450 pregnant women from December 2022 to February 2023. Data included demographic details, PTSD evaluation using the PTSD Checklist-Civilian version (PCL-C) scale, coping mechanisms, and infant growth indicators at three months postpartum. Statistical methods included descriptive statistics, univariate analysis, and multivariate analysis of covariance (ANCOVA) to control for confounding factors. Results: The study enrolled a total of 450 pregnant women. Following exclusions and follow-up attrition, 422 pregnant women were analyzed. There were 72 pregnant women with PTSD and 350 without PTSD. There was no statistically significant difference in birth weight between the two groups of participants. Three months after birth, infants of PTSD-positive mothers exhibited significantly lower weight percentiles at three months (P < 0.001), adjusted for birth weight, feeding method, and coping strategies. Multivariate analysis affirmed a 9.44-point reduction in weight percentile among infants in the PTSD-positive group (95% CI: 4.01–14.87, P = 0.001). Conclusion: Pregnant women suffering from PTSD during the pandemic are at risk of giving birth to infants with impaired physical growth. These findings highlight the critical need for mental health support for pregnant women to foster optimal infant development and to guide public health policies and clinical practices. [ABSTRACT FROM AUTHOR]

Background and objectives: Atypical thymic carcinoids (ATCs) are rare mediastinal malignancies that lack established treatment guidelines. Capecitabine and temozolomide (CapTem) has demonstrated significant efficacy in pancreatic neuroendocrine neoplasms (NENs), while its applicability and effectiveness in ATCs remain underexplored. This study seeks to investigate the efficacy, safety, and prognostic factors associated with CapTem in ATC patients. Design and methods: Thirty-eight ATC patients treated with CapTem at our center were analyzed. We assessed the objective response rate (ORR), disease control rate (DCR), progression-free survival (PFS), overall survival (OS), and treatment-related adverse effects. We also examined patients' clinicopathological characteristics and their correlations with CapTem efficacy. Results: The cohort achieved a 15.8% ORR and 89.5% DCR, with a median PFS of 13.0 months. Multivariate analysis identified the platelet-to-lymphocyte ratio (PLR) as a significant independent prognostic factor for PFS, with a PLR ⩾ 235 associated with shorter PFS (7 months vs. undefined, p = 0.0004). Age was an independent prognostic factor for OS, with patients over 50 years experiencing shorter OS (36 months vs. undefined, p = 0.015). Safety analysis showed rare severe toxicities and no treatment-related fatalities. Conclusion: CapTem is an effective and well-tolerated treatment for ATC patients. Pretreatment PLR and age appear to be potential prognostic markers for CapTem therapy; however, these results warrant validation in larger patient cohorts. Plain language summary: Efficacy, safety and prognostic factors of CapTem in ATCs Previous studies have demonstrated the effectiveness of the Capecitabine plus Temozolomide regimen in patients with pancreatic neuroendocrine neoplasms (NENs), but its potential effectiveness in atypical thymic carcinoids (ATCs) remains underexplored. The cases presented in our study complement previous retrospective series, reinforcing the notion that the CapTem regimen can be a rational and well-tolerated treatment option with favorable responses for patients with ATCs. Importantly, our study is the first to identify pretreatment platelet-to-lymphocyte ratio (PLR) as an independent prognostic biomarker associated with progression-free survival (PFS) in patients with ATCs treated with CapTem. We established the optimal cutoff for PLR as 235. Additionally, our findings revealed that age over 50 years is an independent adverse prognostic factor for overall survival (OS). These findings provide valuable insights for clinicians when selecting patients who are more likely to benefit from the CapTem regimen. [ABSTRACT FROM AUTHOR]

The advancement of cutting-edge energy conversion technologies offers significant potential for addressing environmental challenges, enhancing energy security, improving economic competitiveness, and promoting resource conservation. This progress necessitates the development of advanced electrocatalysts. WOx demonstrates high intrinsic catalytic activity, excellent conductivity, an abundance of active sites, and remarkable stability, positioning it as a promising candidate for electrocatalytic reactions. Recently, there has been swift advancement in the development of WOx-based catalysts for various energy-conversion reactions. This review provides a thorough summary of recent developments in WOx-based catalysts for electrocatalytic reactions, emphasizing their multifunctional roles as active species, electron-transfer carriers, hydrogen spillover carriers, and microenvironment regulators. Moreover, it highlights the applications of WOx-based catalysts across different electrocatalytic reactions, with particular focus on the structure–activity relationship. Finally, the review discusses the challenges and future directions of these technologies, as well as key research areas necessary for achieving large-scale applications. [ABSTRACT FROM AUTHOR]

Background: Acute compartment syndrome is a rare but serious complication following gynecological surgery in the lithotomy position, potentially resulting in permanent neuromuscular dysfunction or limb loss, making early recognition and prompt management essential. Case presentation: A 41-year-old woman underwent a laparoscopic myomectomy for uterine fibroids, during which she was positioned in the high lithotomy and head-down position for 118 min. Six hours post-surgery, she experienced spasmodic pain and swelling in both lower extremities. Thirteen hours after surgery, her symptoms worsened, leading to the diagnosis of acute compartment syndrome in both legs. An emergency bilateral fasciotomy was performed, and the patient fully recovered within two months without any neuromuscular dysfunction. Conclusions: ACS should be an important differential diagnosis for lower extremity pain after gynecologic surgery, especially with prolonged lithotomy positioning. Gynecologists should be vigilant for ACS signs and symptoms to prevent delayed diagnosis. [ABSTRACT FROM AUTHOR]

The electrochemical reduction of nitrate to ammonia (NO3RR) is an effective route to ammonia synthesis with the characteristics of low energy input. However, the complex multi‐electron/proton transfer pathways associated with this reaction may trigger the accumulation of competitive by‐products. Herein, boron (B)‐doped Cu electrode (denoted as B–Cu2O/Cu/CP) as "all‐in‐one" catalyst is prepared by one‐step electrodeposition strategy. Caused by the B doping, the charge redistribution and local coordination environment of Cu2O/Cu species are modulated, resulting in the exposure of active sites on the Cu2O/Cu/CP catalyst. In‐situ Fourier transform infrared spectroscopy and theoretical investigations demonstrate that both Cu2O and Cu sites modulated by B can effectively enhance the adsorption of NO3− and facilitate the conversion of intermediate by‐products, thus promoting the direct reduction of NO3− to NH3. Consequently, a remarkable Faradaic efficiency of 92.74% can be obtained on B–Cu2O/Cu/CP catalyst with minimal accumulation of by‐products. It is expected that this work, based on the heterogeneous B doping, will open a maneuverable and versatile way for the design of effective catalysts. [ABSTRACT FROM AUTHOR]

Immunosuppression is ubiquitous in solid tumors, characterized by few cytotoxic T infiltrations, abundant inflammation enrichment, immunosuppressive cell recruitment, etc., which is especially potentiated by incomplete tumor resection. In this report, multi‐hierarchical Fe single‐atom nanozymes (SAzymes) featuring axially‐coordinated O‐Fe‐N4 active centers are constructed to reshape tumor‐associated macrophages (TAMs) epigenetics via repolarizing their phenotypes, which is highly profitable for mitigating tumor immunosuppression after incomplete radiofrequency ablation (iRFA). Therein, a nitrogen‐doped bamboo‐like carbon nanotube (N‐BCNT) is engineered to coordinatively immobilize Fe atoms, wherein another axial coordination (O) is introduced to further switch non‐polar Fe‐N4 to polar O‐Fe‐N4. The polarization switching of active centers synergies with N doping‐encouraged electron transport, low‐temperature synthesis‐discouraged N loss and large surface area‐unlocked O‐Fe‐N4 enrichment to confer tetra‐enzymic catalysis activities including peroxidase, oxidase, catalase, and glutathione oxidase, maximumly favoring reactive oxygen species (ROS) production and hypoxia reversal. After delivering TAMs polarization‐encouraged drug, toosendanin, TAMs epigenetics are altered and their phenotypes are repolarized to relieve immunosuppression and magnify immune responses, which suppress residual tumor progression after iRFA. Such a comprehensive structure optimization strategy provides a versatile route to design and construct SAzymes‐like drugs with multi‐enzymatical catalysis activities. [ABSTRACT FROM AUTHOR]

Introduction: In the context of rural revitalization strategies, effectively managing rural waste is a critical task for achieving ecological environmental sustainability. The current state of garbage collection and transportation is examined in this paper, focusing on the rural waste management practices in Dongfeng County, Liaoyuan City, Jilin Province, through mathematical modeling. Methods: By analyzing the rural domestic waste collection network, the volume of waste generated at various points is fuzzified using the fuzzy mathematical theory. Results: The results of path optimization for waste collection and transportation were derived from a case study, demonstrating the feasibility and practicality of the rural domestic waste recycling network model. Dicussion: Additionally, sensitivity analysis was conducted to assess the impact of changes on the total cost. Potential solutions for rural waste recycling and treatment are offered in this paper, providing valuable insights for the development of a rural domestic waste recycling network in Jilin Province. [ABSTRACT FROM AUTHOR]

Background: Evidence has accumulated to demonstrate that intestinal microbiome can inhibit viral infection. However, our knowledge of the signaling pathways and identity of specific commensal microbes that mediate the antiviral response is limited. Zebrafish have emerged as a powerful animal model for study of vertebrate-microbiota interactions. Here, a rhabdoviral infection model in zebrafish allows us to investigate the modes of action of microbiome-mediated antiviral effect. Results: We observed that oral antibiotics-treated and germ-free zebrafish exhibited greater spring viremia of carp virus (SVCV) infection. Mechanistically, depletion of the intestinal microbiome alters TLR2-Myd88 signaling and blunts neutrophil response and type I interferon (IFN) antiviral innate immunity. Through 16S rRNA sequencing of the intestinal contents from control and antibiotic(s)-treated fish, we identified a single commensal bacterial species, Cetobacterium somerae, that can restore the TLR2- and neutrophil-dependent type I IFN response to restrict SVCV infection in gnotobiotic zebrafish. Furthermore, we found that C. somerae exopolysaccharides (CsEPS) was the effector molecule that engaged TLR2 to mediate the type I IFN-dependent antiviral function. Conclusions: Together, our results suggest a conserved role of intestinal microbiome in regulating type I IFN antiviral response among vertebrates and reveal that the intestinal microbiome inhibits viral infection through a CsEPS-TLR2-type I IFN signaling axis in zebrafish. 7E-apxbKV4cu6qTqgWcuKA Video Abstract [ABSTRACT FROM AUTHOR]

Alphaviruses are important arthropod-transmitted pathogens of humans and livestock. Getah virus (GETV) is an arthritogenic alphavirus that causes disease in horses and piglets; it also poses a potential threat to humans. A live attenuated vaccine candidate named GETV-3ΔS2-CM1, harbouring a deletion in nonstructural protein 3 and substitutions in the capsid protein, is genetically stable and exhibits robust immunogenicity. It was shown to confer passive protection to piglets born to immunized sows. In mice, a single dose of GETV-3ΔS2-CM1 protected against infection with different strains of GETV, Semliki Forest virus, Ross River virus, o'nyong'nyong virus, chikungunya virus, and Barmah Forest virus. Chimaeras based on the GETV-3ΔS2-CM1 backbone maintained both the attenuated phenotype and high immunogenicity. The safety, efficacy, and ability to induce protection against multiple alphaviruses highlights the potential of GETV-3ΔS2-CM1 and chimaeras using this backbone as promising vaccine candidates. By contributing simultaneously to the wellbeing of animals and humans, our universal next generation vaccine strategy helps to achieve "One Health" goals. Author summary: GETV is a zoonotic mosquito transmitted alphavirus. It is causing infections of livestock and has a potential to harm humans. In the present study, we developed an attenuated variant of GETV. Immunization with this vaccine candidate results in antibody response that protects mice, pigs, and the progeny of immunized mothers against challenge with high dose of GETV. Importantly, the immunization also protects against the challenge with other arthritogenic alphaviruses. Furthermore, we demonstrate that attenuated GETV backbone can be used for construction of chimeric alphaviruses that represent promising vaccine candidates. This study highlights the applicability of attenuated GETV for the prevention and control of multiple arthritogenic alphaviruses in animals under the "One Health" principle. [ABSTRACT FROM AUTHOR]

Lipid metabolism reprogramming has emerged as a hallmark of malignant tumors. Lipids represent a complex group of biomolecules that not only compose the essential components of biological membranes and act as an energy source, but also function as messengers to integrate various signaling pathways. In tumor cells, de novo lipogenesis plays a crucial role in acquiring lipids to meet the demands of rapid growth. Increasing evidence has suggested that dysregulated lipid metabolism serves as a driver of cancer progression. Posttranslational modifications (PTMs), which occurs in most eukaryotic proteins throughout their lifetimes, affect the activity, abundance, function, localization, and interactions of target proteins. PTMs of crucial molecules are potential intervention sites and are emerging as promising strategies for the cancer treatment. However, there is limited information available regarding the PTMs that occur in cancer lipid metabolism and the potential treatment strategies associated with these PTMs. Herein, we summarize current knowledge of the roles and regulatory mechanisms of PTMs in lipid metabolism. Understanding the roles of PTMs in lipid metabolism in cancer could provide valuable insights into tumorigenesis and progression. Moreover, targeting PTMs in cancer lipid metabolism might represent a promising novel therapeutic strategy. [ABSTRACT FROM AUTHOR]

Background: This systematic review and meta-analysis aimed to evaluate the impact of perioperative immunonutrition on postoperative outcomes in patients undergoing pancreaticoduodenectomy (PD). Methods: Conducted a comprehensive search in PubMed, Embase, Cochrane Library, Medline, and Web of Science databases to identify all randomized controlled trials (RCTs) on the topic of immunonutrition and PD. Subsequently screened literature, extracted data, and assessed the risk of bias in the included studies, and finally conducted a meta-analysis using RevMan 5.3 software. Results: The analysis included a total of 10 RCTs with 574 patients, among whom 288 were in the immunonutrition group and 283 in the control group. The meta-analysis revealed a significantly lower incidence of postoperative infection-related complications (OR = 0.45; 95% CI: 0.27–0.74; P = 0.002) and severe postoperative complications (OR = 0.61; 95% CI: 0.38–0.98; P = 0.04) in the immunonutrition group compared to the control group. Additionally, patients in the immunonutrition group had a significantly shorter length of hospital stay (MD= -1.87; 95%CI -3.29 - -0.44; P = 0.01). However, the analysis revealed no statistically significant difference in the overall complication rate between the two groups (P = 0.67). Furthermore, the incidence of specific complications and perioperative mortality rates also did not demonstrate any statistically significant differences (all P > 0.05). Conclusions: Perioperative immunonutrition in PD patients can reduce postoperative infection-related complications, but more high-quality RCTs are needed for further validation. [ABSTRACT FROM AUTHOR]

The urban heat island (UHI) effect, a significant environmental challenge within the global urbanization process, poses severe threats to human health, ecological security, and life safety while also impacting the achievement of the United Nations Sustainable Development Goals. This study proposes a multi-scenario optimization method for urban thermal environments based on local climate zones (LCZs) in Changsha City. The research employs a genetic algorithm to optimize the LCZ quantity structure in order to improve the urban temperature environment. Subsequently, the optimized quantity structure is integrated with the future land use simulation (FLUS) model under multi-scenario constraints to achieve optimal spatial distribution of LCZs, providing scientific guidance for urban planning decision-makers. Results demonstrate that the LCZ-based optimization method can effectively regulate the urban thermal environment and maintain a suitable urban temperature range, offering both theoretical foundation and practical guidance for mitigating UHI effects. [ABSTRACT FROM AUTHOR]

Hepatocellular carcinoma (HCC) remains one of the most prevalent malignant tumors globally, characterized by high incidence and mortality rates. Despite ongoing research, the underlying molecular mechanisms of HCC development are not yet fully understood. Utilizing bioinformatic analysis, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), and Western blot assays, we identified that the expression of specificity protein 1 (Sp1) was significantly elevated in HCC cells compared to normal cells. Knockdown of the Sp1 gene led to a marked reduction in the viability and clonogenic potential of HCC cells. Further investigation revealed that the succinylation level of Sp1 was also increased in HCC cells. The upregulation of Sp1 expression was attributed to its succinylation, mediated by KAT2A, with lysine (K)562 identified as the succinylation site. Additionally, KAT2A and Sp1 were found to influence the upregulation of mTOR phosphorylation. Collectively, these findings suggest that KAT2A-promoted succinylation of Sp1 enhances the proliferative capacity of HCC cells by activating the mTOR pathway, providing a theoretical foundation for potential therapeutic strategies against HCC. [ABSTRACT FROM AUTHOR]

This study profiled global single cell-spatial-bulk transcriptome landscapes of hepatocellular carcinoma (HCC) ecosystem from six HCC cases and a non-carcinoma liver control donor. We discovered that intratumoral heterogeneity mainly derived from HCC cells diversity and pervaded the genome-transcriptome-proteome-metabolome network. HCC cells are the core driving force of taming tumor-associated macrophages (TAMs) with pro-tumorigenic phenotypes for favor its dominant growth. Remarkably, M1-types TAMs had been characterized by disturbance of metabolism, poor antigen-presentation and immune-killing abilities. Besides, we found simultaneous cirrhotic and HCC lesions in an individual patient shared common origin and displayed parallel clone evolution via driving disparate immune reprograms for better environmental adaptation. Moreover, endothelial cells exhibited phenotypically conserved but executed differential functions in a space-dependent manner. Further, the spatiotemporal traits of rapid recurrence niche genes were identified and validated by immunohistochemistry. Our data unravels the great significance of HCC cells in shaping vibrant tumor ecosystems corresponding to clinical scenarios. [ABSTRACT FROM AUTHOR]

Recently emerging perovskite nanocrystals (PNCs) have drawn considerable attention due to the superior optoelectronic performances. However, the terrible stability of red emissive PNCs (r‐PNCs) limits their broader application in aqueous media. Despite various encapsulation methods are proposed, the preparation of aqueous r‐PNCs with facile operation and excellent durability is still challenging. Herein, aqueous green emissive PNCs (g‐PNCs) by employing water as an external stimulus to treat oleyamine‐capped CsPbBr3 PNCs are synthesized initially, and then aqueous‐stable pure red emissive PNCs by anion‐exchange reaction and subsequently thiocyanate (SCN‐) passivation treatment (SCN‐r‐PNCs) are prepared. The obtained aqueous‐stable SCN‐r‐PNCs display high brightness, outstanding stability, and extended photoluminescence lifetime. Moreover, by combining aqueous r‐PNCs and portable smartphones, a fluorescence chemosensor for the detection of aqueous SCN− ions is constructed, which exhibits simple operation, high sensitivity, high selectivity, low detection limit, and visualized sensing. This work not only provides a new and facile strategy to prepare aqueous‐stable red emissive PNCs, but also further extends the application of red emissive PNCs in aqueous‐based fields. [ABSTRACT FROM AUTHOR]

Diverging from air breakdown‐based triboelectric nanogenerators (TENGs), recent TENG designs present high output power density without requiring precise control over discharge channels. However, existing researches predominantly ascribe its direct current output to electrostatic induction, disregarding the critical factor of charge leakage. This oversight hampers efforts to improve device performance, especially in material selection and optimization. Here, the generation of direct current signals ultimately stems from material charge leakage and spatial electrostatic induction is illustrated. Through theoretical analysis, visualization, and experimental measurement of four phenomena in the device, a quadruple‐effect mechanoelectrical conversion mechanism is established to refine the material selection rule. Under this guideline, the output power density is increased by 34.42% in contrast to the electrostatic induction direct current TENG. For practical applications, a power management circuit is utilized to boost the device's charging rate by up to 18 times. Furthermore, the high voltage of the device can activate discharge‐type UV tubes, demonstrating great potential in developing self‐powered wastewater treatment systems. The multiple charge behaviors proposed in this work, along with the material selection rule, lay a solid foundation for achieving high output power density in direct current TENGs. [ABSTRACT FROM AUTHOR]

Background: Recurrent gliomas rapidly progress and have high mortality and poor prognosis in the central nervous system. Therefore, further investigation of prognostic and therapeutic markers is needed. Methods: The mRNA expression, clinical data, and coagulation-related genes (CRGs) associated with recurrent glioma were obtained and calculated from the Chinese Glioma Genome Atlas and Kyoto Encyclopedia of Genes and Genomes databases. The significant CRGs were calculated by Weighted gene co-expression network analysis and PPI network. A prediction model was constructed using the least absolute shrinkage and selection operator regression analysis. Recurrent gliomas were stratified into high and low-risk groups based on the median risk score (RS). The Kaplan–Meier curve was used to analyze the difference in overall survival (OS) between these groups, while the receiver operating characteristic (ROC) curve was used to evaluate the accuracy of the gene model at 1-, 3-, and 5-years. Clinical factors, including age, gender, MGMT methylation status, radiotherapy, chemotherapy, and RS, were included in the univariate and multivariate regression analysis. A prognostic nomogram and calibration curve were established based on these factors. Results: Overall, seven CRGs associated with the prognosis were identified, including BTK, ITGB1, GNAI3, CFH, LYN, CFI, and F3. OS and survival rates were lower in the high-risk group compared with the low-risk group. The ROC curve demonstrated the area under the curve values >0.65 at 1-, 3-, and 5-years, confirming the reliability of the prognostic model. The univariate regression analysis indicated that tumor grade (grades 2, 3, and 4), histopathology (GBM or not), chemotherapy, IDH mutation, and 1p19q co-deletion status were independent prognostic indicators. Univariate and multivariate regression analyses indicated that RS was an independent prognostic factor for patients with recurrent glioma. Immune analysis revealed low infiltration of resting dendritic cells and high expression of programmed death receptor 1 in the high-risk group. Conclusion: This study comprehensively investigated the correlation between CRGs and recurrent glioma prognosis, offering crucial insights for further research into glioma recurrence mechanisms and treatment strategies. [ABSTRACT FROM AUTHOR]

Background: This study investigated the factors influencing the prognosis of children with steroid-resistant nephrotic syndrome (SRNS) in patients from the Guangxi region. Methods: We retrospectively analyzed clinical and pathological data of 279 patients with SRNS from six tertiary hospitals in Guangxi. Clinical data were compared between initial (I-SRNS) and secondary (S-SRNS) steroid resistance subgroups and Cox regression analysis was used to determine risk factors for chronic kidney disease (CKD) and CKD stage 5 (CKD5) in patients with SRNS. Results: The median age of onset was 54 months. Thirty-three patients had extra-kidney manifestations. Fifty-two, 24, 57, 33, and 41 patients had hypertension, acute kidney injury, vitamin D deficiency, high intraocular pressure, and dwarfism, respectively. One hundred eighty-two and 92 patients had I-SRNS and S-SRNS, respectively. There were significant differences in sex, ethnicity, family history, incidence of hematuria, clinical classification, efficacy of immune agents, and prognosis between groups (P < 0.05). Among the 279 cases of SRNS, 239 had normal kidney function, 37 developed CKD, and 16 had CKD5. An increase in serum creatinine level (HR = 1.003) was significantly associated with CKD in children with SRNS, and effective immunosuppressant therapy decreased the CKD risk (HR = 0.168). Patients with increased serum creatinine levels (HR = 1.003) and acute kidney injury (HR = 4.829) were more likely to progress to CKD5. Conclusions: Children with S-SRNS showed a higher response to immunosuppressants than those with I-SRNS. Effective immunosuppressant therapy was found to protect against CKD, whereas increased acute kidney injury was an independent risk factor for CKD5. [ABSTRACT FROM AUTHOR]