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Survival of olfactory mucosal mesenchymal stem cells (OM-MSCs) remains the low level in the cerebral microenvironment during intracerebral hemorrhage (ICH). This article aims to reveal the differential expression profile of ICH-stimulated OM-MSCs based on whole transcriptome sequence analysis. OM-MSCs were isolated from 6-week C57BL/6 mice. Morphology and surface markers of OM-MSCs were investigated by light microscope and flow cytometry, respectively. OM-MSCs were incubated with 20 U/mL thrombin for 24 h to mimic ICH-induced injury in vitro. Total RNA was extracted for whole transcriptome sequencing and qPCR. OM-MSCs were characterized by negative for CD45 and CD34, and positive for CD44, CD90 and CD29. Thrombin led to decrease in cell viability and increase in senescence and apoptosis in OM-MSCs. In total, 736 lncRNAs (upregulated: 393; downregulated: 343), 21 miRNAs (upregulated: 7; downregulated: 14) and 807 mRNAs (upregulated: 422; downregulated: 385) were identified. GO and KEGG pathways were enriched in protein heterodimerization activity, trans-synaptic signaling, membrane pathway, alcohol metabolic process, organic hydroxy compound biosynthesis process, secondary alcohol metabolic process, alcoholism, neutrophil extracellular trap formation, systemic lupus erythematosus, metabolic process, steroid biosynthesis and drug metabolism-cytochrome P450. 200 lncRNA-miRNA-mRNA were predicted in thrombin-induced OM-MSCs. Based on qPCR, we validated COMMD1B, MOAP1, lncRNA CAPN15, lncRNA ALDH1L2, miR-3473b and miR-1964-3p were upregulated in thrombin-stimulated OM-MSCs, and GM20431, lncRNA GAPDH and miR-122b-3p were downregulated. Our findings provide novel understanding for thrombin-induced injury in OM-MSCs. Differently-expressed RNAs can be the targets of improving therapeutic application of OM-MSCs. [ABSTRACT FROM AUTHOR]

Background: Mechanical ventilation (MV) can cause diaphragmatic injury and ventilator induced diaphragmatic dysfunction (VIDD). Diaphragm ultrasonography (DU) is increasingly used to assess diaphragmatic anatomy, function and pathology of patients receiving MV in the pediatric intensive care unit (PICU). We report the poor contractile ability of diaphragm during ventilation of critically ill patients in our PICU and the association to prolonged length of MV and PICU stay. Methods: Patients who received MV within 24 h of admission to the PICU, expected to undergo continuous MV for more than 48 h and succeeded to extubate were included in the study. DU monitoring was performed daily after the initiation of MV until extubation. Diaphragm thickening fraction (DTF) measured by DU was used as an indicator of diaphragmatic contractile activity. Patients with bilateral DTF = 0% during DU assessment were allocated into the severe VIDD group (n = 26) and the rest were into non-severe VIDD group (n = 29). The association of severe VIDD with individual length of MV, hospitalization and PICU stay were analyzed. Results: With daily DU assessment, severe VIDD occurred on 2.9 ± 1.2 days after the initiation of MV, and lasted for 1.9 ± 1.7 days. Values of DTF of all patients recovered to > 10% before extubation. The severe VIDD group had a significantly longer duration (days) of MV [12.0 (8.0-19.3) vs. 5.0 (3.5–7.5), p < 0.001] and PICU stay (days) [30.5 (14.9–44.5) vs. 13.0 (7.0-24.5), p < 0.001]. The occurrence of severe VIDD, first day of severe VIDD and length of severe VIDD were significantly positively associated with the duration of MV and PICU stay. The occurrence of severe VIDD on the second and third days after initiation of MV significantly associated to longer PICU stay (days) [43.0 (9.0–70.0) vs. 13.0 (3.0–40.0), p = 0.009; 36.0 (17.0-208.0) vs. 13.0 (3.0–40.0), p = 0.005, respectively], and the length of MV (days) was significantly longer in those with severe VIDD on the third day after initiation of MV [16.5 (7.0–29.0) vs. 5.0 (2.0–22.0), p = 0.003]. Conclusions: Daily monitoring of diaphragmatic function with bedside ultrasonography after initiation of MV is necessary in critically ill patients in PICU and the influences and risk factors of severe VIDD need to be further studied. (355 words) [ABSTRACT FROM AUTHOR]

This study investigates the superplastic deformation of a cold-rolled Ni/Graphene Oxide composite foil, with a particular emphasis on the mechanism that enhances the two-dimensional ultra-thin phase graphene oxide (GO). The findings reveal that intergranular fracture takes place due to a larger plastic strain in the radial direction than in the tangential direction. Furthermore, the reinforcing phase GO tends to become fibrous after rolling, with the fiber direction oriented along the radial direction. The size of matrix grains surrounded by GO does not significantly increase at high temperatures. However, during stretching, the formed voids expand along the matrix and both ends of the GO. The formation and aggregation of voids increases the likelihood of high-temperature tensile fracture of the foil, ultimately resulting in a lower elongation of Ni/GO after rolling when compared to the original foil. The two-dimensional flexible reinforcement phase tends to transform from sheet-like to fibrous. When an external force is applied, it causes the multi-layer stacked GO to appear tense, which is visually observed for the first time. [ABSTRACT FROM AUTHOR]

Background: Metagenomic next-generation sequencing (mNGS) excels in diagnosis of infection pathogens. We aimed to evaluate the performance of mNGS for the diagnosis of Pneumocystis jirovecii pneumonia (PJP) in non-HIV infected children. Methods: Totally 36 PJP children and 61 non-PJP children admitted to the pediatric intensive care unit from March 2018 to December 2021 were retrospectively enrolled. Clinical features of PJP children were summarized. 1,3-β-D glucan (BDG) test and bronchoalveolar lavage fluid (BALF) mNGS were used for evaluation of PJP diagnostic performance. Antimicrobial management modifications for PJP children after the mNGS results were also reviewed. Results: Pneumocystis jirovecii was detected in all PJP children by mNGS (36/36), and the sensitivity of mNGS was 100% (95% confidence interval [CI]: 90.26–100%). The sensitivity of BDG was 57.58% (95% CI: 39.22–74.52%). Of the 26 (72.2%) PJP patients with mixed infection, twenty-four (66.7%) were detected by BALF-mNGS. Thirteen patients (36.1%) had their antimicrobial management adjusted according to the mNGS results. Thirty-six PJP children included 17 (47.2%) primary immunodeficiency and 19 (52.8%) secondary immunodeficiency, of whom 19 (52.8%) survived and 17 (47.2%) died. Compared to survival subgroup, non-survival subgroup had a higher rate of primary immunodeficiency (64.7% vs. 31.6%, P = 0.047), younger age (7 months vs. 39 months, P = 0.011), lower body weight (8.0 kg vs. 12.0 kg, P = 0.022), and lower T lymphocyte counts. Conclusions: The mortality rate of PJP in immunosuppressed children without HIV infection is high and early diagnosis is challenging. BALF-mNGS could help identify PJP and guide clinical management. [ABSTRACT FROM AUTHOR]

To mitigate the decrease in mechanical performance of Sn58Bi/Cu solder joints resulting from electromigration-induced damage. The CeO2 nanoparticles were incorporated into Sn58Bi solder by a melt-casting method, and their effects on the microstructure and properties of Sn58Bi/Cu solder joints under electromigration were investigated. The study results demonstrate that the addition of 0.125 ~ 0.5 wt% CeO2 nanoparticles refines the eutectic microstructure of Sn58Bi solder alloy. At an addition amount of 0.5 wt%, the composite solder alloy exhibits the maximum tensile strength of 68.9 MPa, which is 37% higher than that of the base solder. CeO2 nanoparticle-reinforced Sn58Bi solder can achieve excellent solderbility with Cu substrates and the joints can significantly inhibit the growth of the anodic Bi-rich layer, which is responsible for electromigration. With the extension of current stressing time, Bi-rich and Sn-rich layer are respectively formed on the anode and cathode in the joints. The intermetallic compound (IMC) layer grows asymmetrically, transitioning from a fan-shaped morphology to a flattened structure at the anode and to a thickened mountain-like morphology at the cathode. Adding the CeO2 nanoparticles helps to mitigate the decrease in mechanical performance caused by electromigration damage during current application to some extent. Over the current stressing period of 288 ~ 480 h, the fracture position shifts from the anodic IMC/Bi-rich interface to the cathodic Sn-rich/IMC interface. The fracture mechanism transitions from a brittle fracture characterized by plate-like cleavage to a ductile–brittle mixed fracture with fine dimples and cleavage. [ABSTRACT FROM AUTHOR]

Background: This study investigated the relaxation effect of PGE2 on the ureter and its role in promoting calculi expulsion following calculi development. Methods: By using immunofluorescence and Western blot, we were able to locate EP receptors in the ureter. In vitro experiments assessed the impact of PGE2, receptor antagonists, and agonists on ureteral relaxation rate. We constructed a model of ureteral calculi with flowable resin and collected ureteral tissue from postoperative side of the ureter after obstruction surgery. Western blot analysis was used to determine the protein expression levels of EP receptors and the PGE2 terminal synthase mPGES-1. Additionally, PGE2 was added to smooth muscle cells to observe downstream cAMP and PKA changes. Results: The expression of EP2 and EP4 proteins in ureteral smooth muscle was verified by Western blot analysis. According to immunofluorescence, EP2 was primarily found on the cell membrane, while EP4 was found in the nucleus. In vitro, PGE2 induced concentration-dependent ureteral relaxation. Maximum diastolic rate was 70.94 ± 4.57% at a concentration of 30µM. EP2 antagonists hindered this effect, while EP4 antagonists did not. Obstructed ureters exhibited elevated mPGES-1 and EP2 protein expression (P < 0.01). Smooth muscle cells treated with PGE2 displayed increased cAMP and phosphorylated PKA. Conclusions: PGE2 binding to EP2 induces ureteral relaxation through the cAMP-PKA pathway. This will provide a new theoretical basis for the development of new therapeutic approaches for the use of PGE2 in the treatment of ureteral stones. [ABSTRACT FROM AUTHOR]

Summary: During horizontal drilling operations, real‐time prediction of drilling pressure during the drilling process can help the drilling team cope with the complex and changing working environment downhole, adjust the parameters of the drilling rig promptly, make correct decisions, reduce the probability of drilling accidents, and avoid affecting the duration and cost of the project. This study provides a method for real‐time prediction of the drilling pressure of horizontal drilling rigs. A deep learning model based on a convolutional Transformer is trained for accurate real‐time prediction by extracting real‐time operating data of the horizontal drilling rig from the data acquisition system. The method proposed in this study can be a useful tool to improve the performance of horizontal drilling rigs and can assist the drilling team in operating horizontal drilling rigs. The results of the case study show that: (1) the proposed convolutional Transformer model provides reliable real‐time prediction with an MAE of 0.304 MPa and an RMSE of 0.508 MPa; (2) the proposed method can quickly and accurately predict the trend of drilling pressure change in the next period based on the current change of drilling pressure, and grasp the dynamics of drilling pressure of horizontal drilling rigs in advance. Further research could focus on assisted decision‐making and intelligent optimization to provide solutions for preventing drilling accidents and improving horizontal rig performance based on the prediction. [ABSTRACT FROM AUTHOR]

Background: Antimicrobial resistance (AMR) is a major threat to children's health, particularly in respiratory infections. Accurate identification of pathogens and AMR is crucial for targeted antibiotic treatment. Metagenomic next-generation sequencing (mNGS) shows promise in directly detecting microorganisms and resistance genes in clinical samples. However, the accuracy of AMR prediction through mNGS testing needs further investigation for practical clinical decision-making. Methods: We aimed to evaluate the performance of mNGS in predicting AMR for severe pneumonia in pediatric patients. We conducted a retrospective analysis at a tertiary hospital from May 2022 to May 2023. Simultaneous mNGS and culture were performed on bronchoalveolar lavage fluid samples obtained from pediatric patients with severe pneumonia. By comparing the results of mNGS detection of microorganisms and antibiotic resistance genes with those of culture, sensitivity, specificity, positive predictive value, and negative predictive value were calculated. Results: mNGS detected bacterial in 71.7% cases (86/120), significantly higher than culture (58/120, 48.3%). Compared to culture, mNGS demonstrated a sensitivity of 96.6% and a specificity of 51.6% in detecting pathogenic microorganisms. Phenotypic susceptibility testing (PST) of 19 antibiotics revealed significant variations in antibiotics resistance rates among different bacteria. Sensitivity prediction of mNGS for carbapenem resistance was higher than penicillins and cephalosporin (67.74% vs. 28.57%, 46.15%), while specificity showed no significant difference (85.71%, 75.00%, 75.00%). mNGS also showed a high sensitivity of 94.74% in predicting carbapenem resistance in Acinetobacter baumannii. Conclusions: mNGS exhibits variable predictive performance among different pathogens and antibiotics, indicating its potential as a supplementary tool to conventional PST. However, mNGS currently cannot replace conventional PST. [ABSTRACT FROM AUTHOR]

Background: Sepsis-induced myocardial injury is a serious complication of sepsis. QT prolongation is a proarrhythmic state which reflects myocardial injury in a group of heterogeneous disorders. However, the study on the clinical value of QT prolongation in sepsis is limited. Methods: We aimed to investigate the clinical characteristics and predictors of new-onset QT prolongation in sepsis and its impact on the outcome in a multicenter retrospective cohort study. Electrocardiographic and clinical data were collected from patients with sepsis from the wards and intensive care units of four centers after exclusion of QT-influencing medications and electrolyte abnormalities. Clinical outcomes were compared between patients with and without QT prolongation (QTc > 450 ms). Multivariate analysis was performed to ascertain whether QT prolongation was an independent predictor for 30-day mortality. The factors predicting QT prolongation in sepsis were also analyzed. Results: New-onset QT prolongation occurred in 235/1024 (22.9%) patients. The majority demonstrated similar pattern as type 1 long QT syndrome. Patients with QT prolongation had a higher 30-day in-hospital mortality (P < 0.001), which was also associated with increased tachyarrhythmias including paroxysmal atrial fibrillation or tachycardia (P < 0.001) and ventricular arrhythmia (P < 0.001) during hospitalization. QT prolongation independently predicted 30-day mortality (P = 0.044) after multivariate analysis. History of coronary artery disease (P = 0.001), septic shock (P = 0.008), acute respiratory (P < 0.001), heart (P = 0.021) and renal dysfunction (P = 0.013) were independent predictors of QT prolongation in sepsis. Conclusions: New-onset QT prolongation in sepsis was associated with increased mortality as well as atrial and ventricular arrhythmias, which was predicted by disease severity and organ dysfunction. [ABSTRACT FROM AUTHOR]

Metformin/glipizide tablets are a compound formulation composed of metformin hydrochloride and glipizide. This study aimed to assess the pharmacokinetics and bioequivalence of two fixed-dose combination (FDC) tablets of metformin/glipizide (500 mg/2.5 mg) in healthy Chinese subjects. We conducted a single-center, open-label, randomized, two-way crossover study with a total of 48 subjects enrolled (24 in each dietary group). The test or reference formulations were given to the subjects at random at a ratio of 1 : 1, with a seven-day washout period. Blood samples, collected at prearranged intervals before and up to 24 hours after dosage, were analyzed using validated LC-MS/MS technology to ascertain plasma concentrations of metformin and glipizide. Finally, 23 subjects completed the fasting and fed studies, respectively. In both studies, the 90% confidence intervals for the geometric mean ratios (test/reference) of the C m ax , AUC0-t, and AUC0-∞ were all found to fall within the acceptable range for bioequivalence (80%–125%). The exposure of metformin/glipizide FDC tablets in vivo was not significantly affected by food. No serious adverse events were observed. In conclusion, this study demonstrated that both the test and reference metformin/glipizide tablets exhibited bioequivalence and were well tolerated under both fasting and fed conditions. This trial is registered with CTR202686. [ABSTRACT FROM AUTHOR]

Background: Anaesthetic methods and drugs with rapid onset, rapid recovery and better postoperative analgesia are more suitable for rapid recovery in obstetric anaesthesia. We formulated the following hypothesis: a combination of mepivacaine and ropivacaine could provide a longer analgesic effect and have more advantages in terms of rapid-recovery indicators. Methods: A total of 180 pregnant women scheduled to undergo elective caesarean sections were randomly assigned to three surgical groups, which received 2% mepivacaine (Group M), 2% mepivacaine + 0.75% ropivacaine (Group MR) (Volume 1:1) or 0.75% ropivacaine (Group R) through an epidural catheter. The situation of postoperative analgesia and other indicators of rapid recovery were recorded. Results: One hundred and fifty patients were included in the final analysis. Their demographic data were similar. The visual analogue scale (VAS) scores of Group MR and Group R were lower than Group M at 1 and 2 h after surgery both at rest and with movement (P < 0.05), and the time to first ambulation in Group MR (17.38 ± 2.06 h) and Group M (17.20 ± 2.09 h) was shorter than that in Group R (22.18 ± 1.74 h) (P < 0.05). Conclusion: Application of 2% mepivacaine combined with 0.75% ropivacaine for epidural anaesthesia can provide longer postoperative analgesia and earlier ambulation, these effect may be more suitable than that of 2% mepivacaine or 0.75% ropivacaine alone for caesarean section. Trial registration: This study was registered at Chinese Clinical Trial Registry (Registration number: ChiCTR 2300078288; date of registration: 04/12/2023). [ABSTRACT FROM AUTHOR]

Blonanserin is a novel oral antischizophrenic drug. Under fasting (n = 50) and fed (n = 60) conditions, this study compared the bioequivalence of the generic blonanserin tablet with the reference blonanserin tablet. In this single‐center, randomized, open‐label, 2‐period, 2‐sequence, crossover study, 110 patients were randomly given a 4‐mg dose of either the test or reference blonanserin tablet with a 14‐day washout period. Blood samples were taken before performing and up to 72 hours following. A validated high‐performance liquid chromatography‐tandem mass spectrometry technique was used to measure the levels of blonanserin in plasma. Safety was evaluated throughout the study. The study found no significant differences in the maximum observed drug concentration in the plasma (Cmax), the area under the plasma concentration‐time curve from time 0 to the last sampling time (AUC0‐t), and the area under the plasma concentration‐time curve from time 0 to infinity (AUC0‐∞) between the 2 blonanserin formulations. The 90% confidence intervals of the geometric mean ratio of the test/reference formulations for Cmax, AUC0‐t, and AUC0‐∞ were within the 80%‐125% limit. Food dramatically raised blonanserin exposure, and also significantly prolonged the lag time of absorption. No serious adverse events occurred. These results indicate that the 2 blonanserin formulations were bioequivalent and well tolerated in healthy Chinese subjects. In clinical treatment, it is necessary to consider the food effect of blonanserin. [ABSTRACT FROM AUTHOR]

Aims: Status epilepticus (SE) is the most common neurological emergency in pediatric patients. This study aimed to screen for prognostic biomarkers of SE in the cerebrospinal fluid (CSF) using metabolomics. Methods: Ultra‐performance liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry (UPLC‐QTOF‐MS) was conducted to identify prognostic biomarkers in CSF metabolomics by comparing the poor outcome group (N = 13) with the good outcome group (N = 15) of children with SE. Differentially expressed metabolites were identified using Mann–Whitney U test corrected by Benjamini‐Hochberg and partial least squares discriminant analysis (PLS‐DA). Results: The PLS‐DA model identified and validated significant metabolic differences between the poor and good outcome groups of children with SE (PLS‐DA with R2Y = 0.992 and Q2 = 0.798). A total of 49 prognosis‐related metabolites were identified. Of these metabolites, 20 including glutamyl‐glutamine, 3‐iodothyronamine, and L‐fucose had an area under the curve (AUC) ≥ 80% in prognostic prediction of SE. The logistic regression model combining glutamyl‐glutamine and 3‐iodothyronamine produced an AUC value of 0.976, with a sensitivity of 0.863 and specificity of 0.956. Pathway analysis revealed that dysregulation of the citrate cycle (TCA) and arginine biosynthesis may contribute to poor SE prognosis. Conclusions: This study highlighted the prognosis‐related metabolomic disturbances in the CSF of children with SE and identified potential prognostic biomarkers. A prognostic prediction model combining glutamyl‐glutamine and 3‐iodothyronamine with high predictive value was established. [ABSTRACT FROM AUTHOR]

Summary: Aiming at current serious problems that the bolt looseness can cause heavy damages and the manual bolt looseness detection is ineffective, this study presents a bolt looseness detection method based on Canny edge detector. First, HSV (hue, saturation, value) color space is used to extract the area of mark bar. Then, after expanding, eroding and median filtering the image, Canny edge detector is used to segment the marker area to extract its edge. Last, the fitted slope of edge is used to judge whether the bolt is loose. The experimental results show that the proposed method has F1 score of 88.37%, contributing to the automated detection of bolt looseness. [ABSTRACT FROM AUTHOR]

Electromigration is one of the most important research issues affecting the reliability of solder joints. Current-induced Joule heating affects the electromigration behavior of solder joints. Solder joints with different cross-sectional areas were designed to obtain different Joule heating properties. The effects of the interfacial intermetallic compound (IMC) and mechanical properties of Sn58Bi/Cu solder joints were studied for different Joule heating properties. The results showed that as the cross-sectional area of the Sn58Bi/Cu solder joints increased, the Joule heating of the joint increased. The anode IMC thickness of the joint thickened and transformed into a planar shape. The Bi migrated to the anode region to form a Bi-rich layer and gradually increased in thickness. The cathode IMC thickness first increased, then decreased, and gradually dissolved. The Sn-rich layer formed near the solder side and gradually increased in thickness, with microcracks occurring when the cross-sectional area of the joint increased to 0.75 mm2. The joint shear fracture path moved from the soldering zone near the cathode IMC layer to the interfacial IMC layer. The fracture mechanism of the joint changed from a mixed brittle/tough fracture, dominated by deconstruction and secondary cracking, to a brittle fracture dominated by deconstruction. The joint shear strength was reduced by 60.9% compared to that in the absence of electromigration. [ABSTRACT FROM AUTHOR]

Sepsis is a critical condition affecting patients worldwide. Systemic inflammatory response syndrome in sepsis contributes to organ dysfunction and mortality. The oXiris is a recently developed continuous renal replacement therapy (CRRT) hemofilter indicated for the adsorption of cytokines from the bloodstream. In our study, in a septic child, CRRT with three filters, including the oXiris hemofilter, resulted in a downregulation of inflammatory biomarkers and a reduction of vasopressors. Herein, we described the first report of such usage in septic children. [ABSTRACT FROM AUTHOR]

Tunnel Boring Machines (TBMs) have been used in many underground coal mines in China with high constructive efficiency, sound equipment integration and low cost. A recent application of TBM in Yuandian No.2 Coal Mine in Huaibei, China, has shown that the surrounding rockmass spalled severely in stratum impacted by multiple joint sets. To analyze this problem, a new 3D ubiquitous-multiple-joint (UMJ) model was proposed in the present research, which comprised a rock matrix and multiple sets of joints according to geological conditions. Then, the yielding and deformation of the roadway excavated by TBM were analyzed. The results showed that the plastic zones, stress states and displacement of surrounding rockmass were significantly affected by the orientation of joint sets. The results could well indicate failure characteristics of the in-situ roadway and showed the advantages of UMJ model to simulate the excavation in jointed rockmass. The key factors influencing the roadway stability were discussed by the UMJ model. Based on the simulation results, suggestions on excavation-support schemes were proposed for TBM excavating roadways. Highlights: 3D Ubiquitous-Multiple-Joint model is proposed to handle rockmass with multiple joint sets The stability of roadway is significantly affected by orientation and strength of joint sets Some measures are suggested to control TBM excavated roadway in jointed stratum [ABSTRACT FROM AUTHOR]

To improve the stability of the bridge structure, we detect bolts in the bridge which cause the symmetry failure of the bridge center. For data acquisition, bolts are small-scale objects under complex background in images, and their feature expression ability is limited. Due to those questions, we propose a new bolt positioning detection based on improved YOLOv5 for bridge structural health monitoring. This paper makes three major contributions. Firstly, according to the calibration anchor boxes of bolts, the size and proportion parameters of the initial anchor boxes are optimized by K-means++ clustering algorithm to solve the initial clustering problem of anchor boxes in object detection. Second, the hypercolumn (HC) technique fuses the low-level global features of the trunk and the high-level local features of three different scales to solve the problem of the inefficient distribution of anchors and insufficient extraction of classification features. In this way, we improve the detection accuracy and speed of bolt detection. Finally, we establish a dataset of bridge bolts through network collection and public datasets, including 1494 images. We compare and verify the new method in the collected bolt dataset. The experimental results show that the precision (P) of the improved YOLOv5x is up to 87.3%, and the average precision (AP) is up to 86.3%, which are 6.5% and 5.9% higher than the original YOLOv5x, respectively. [ABSTRACT FROM AUTHOR]

Unmanned aerial vehicles (UAVs) have attracted increasing attention in recent years because of their broad range of applications in city security, military reconnaissance, disaster rescue, and so on. As one of the critical algorithms in the field of artificial intelligence, object tracking greatly improves the working efficiency of UAVs. However, unmanned aerial vehicle (UAV) object tracking still faces many challenges. UAV objects provide limited textures and contours for feature extraction due to their small sizes. Moreover, to capture objects continuously, a UAV camera must constantly move with the object. The above two reasons are usual causes of object-tracking failures. To this end, we propose an end-to-end discriminative tracker called TMDiMP. Inspired by the self-attention mechanism in Transformer, a novel memory-aware attention mechanism is embedded into TMDiMP, which can generate discriminative features of small objects and overcome the object-forgetting problem after camera motion. We also build a UAV object-tracking dataset with various object categories and attributes, named VIPUOTB, which consists of many video sequences collected in urban scenes. Our VIPUOTB is different from other existing datasets in terms of object size, camera motion speed, location distribution, etc. TMDiMP achieves competitive results on our VIPUOTB dataset and three public datasets, UAVDT, UAV123, and VisDrone, compared with state-of-the-art methods, thus demonstrating the effectiveness and robustness of our proposed algorithm. [ABSTRACT FROM AUTHOR]

Ships comprise the only and most important ocean transportation mode. Thus, ship detection is one of the most critical technologies in ship monitoring, which plays an essential role in maintaining marine safety. Optical remote-sensing images contain rich color and texture information, which is beneficial to ship detection. However, few optical remote-sensing datasets are open publicly due to the issue of sensitive data and copyrights, and only the HRSC2016 dataset is built for the ship-detection task. Moreover, almost all general object detectors suffer from the failure of multi-scale ship detection because of the diversity of spatial resolution and ship size. In this paper, we re-annotate the HRSC2016 dataset and supplement 610 optical remote-sensing images to build a new open source ship-detection benchmark dataset with rich multi-scale ship objects named the HRSC2016-MS dataset. In addition, we further explore the potential of a recursive mechanism in the field of object detection and propose a novel multi-scale ship-detection framework (MSSDet) in optical remote-sensing images. The success of detecting multi-scale objects depends on the hierarchical pyramid structure in the object-detection framework. However, the inherent semantic and spatial gaps among hierarchical pyramid levels seriously affect detection performance. To alleviate this problem, we propose a joint recursive feature pyramid (JRFP), which can generate semantically strong and spatially refined multi-scale features. Extensive experiments were conducted on the HRSC2016-MS, HRSC2016, and DIOR datasets. Detailed ablation studies directly demonstrated the effectiveness of the proposed JRFP architecture and also showed that the proposed method has excellent generalizability. Comparisons with state-of-the-art methods showed that the proposed method achieves competitive performance, i.e., 77.3%, 95.8%, and 73.3% mean average precision accuracy on the HRSC2016-MS, HRSC2016, and DIOR datasets, respectively. [ABSTRACT FROM AUTHOR]

MXenes are promising supports for anchoring metal single atoms due to their versatile composition, well-defined nanostructures, and suitable conductivity. However, metal single atoms are usually coordinated with surface terminal groups (-O, -OH, -Cl, etc.) of MXenes via conventional wet-impregnation, resulting in limited electronic structure modification. Through a NiCl2 molten salt etching method, we observed that Ni single atoms could be in-situ doped in the lattice of MXenes analogue TiC0.5N0.5 support (denoted as Ni1/TiC0.5N0.5), resulting in much larger charge transfer from Ni atoms to adjacent Ti atoms, and thus increasing the electronic density of these Ti atoms. When used for NO2 sensing, Ni1/TiC0.5N0.5 exhibited excellent response sensitivity (ultra-low limit of detection ∼ 10 ppb), selectivity, and good stability at room temperature. This study provides an effective strategy for producing MXenes analogue supported metal single atoms for potential application in gas sensing. [ABSTRACT FROM AUTHOR]

For single-atom catalysts (SACs), the catalyst supports are not only anchors for single atoms, but also modulators for geometric and electronic structures, which determine their catalytic performance. Selecting an appropriate support to prepare SACs with uniform coordination environments is critical for achieving optimal performance and clarifying the relationship between the structure and the property of SACs. Approaching such a goal is still a significant challenge. Taking advantage of the strong d-π interaction between Cu atoms and diacetylenic in a graphdiyne (GDY) support, we present an efficient and simple strategy for fabricating Cu single atoms anchored on GDY (Cu1/GDY) with uniform Cu1-C4 single sites under mild conditions. The Cu atomic structure was confirmed by combining synchrotron radiation X-ray absorption spectroscopy, X-ray photoelectron spectroscopy and density functional theory (DFT) calculations. The as-prepared Cu1/GDY exhibits much higher activity than state-of-the-art SACs in direct benzene oxidation to phenol with H2O2 reaction, with turnover frequency values of 251 h−1 at room temperature and 1889 h−1 at 60°C, respectively. Furthermore, even with a high benzene conversion of 86%, high phenol selectivity (96%) is maintained, which can be ascribed to the hydrophobic and oleophyllic surface nature of Cu1/GDY for benzene adsorption and phenol desorption. Both experiments and DFT calculations indicate that Cu1-C4 single sites are more effective at activating H2O2 to form Cu=O bonds, which are important active intermediates for benzene oxidation to phenol. [ABSTRACT FROM AUTHOR]

Owing to unique alkyne‐rich structure, graphdiyne (GDY) has been proven to be a superb support for anchoring metal catalysts. Herein we demonstrate a new role of GDY as the wettability modifier for enhanced hydrogenation catalysis. After loading a certain amount GDY nanospheres, the silica mesoporous channels become superaerophilic, which allows gaseous H2 to be directly stored inside, thus significantly increasing the H2 concentration around the palladium nanoparticles (NPs). At the same time, GDY nanospheres also alter the electronic structure of the Pd NPs via a strong d‐π interaction. Combining these two roles of GDY, allows the hydrogenation of benzaldehyde to proceed under ambient H2 pressure in water, with an impressive 4.3‐fold enhancement compared to the unmodified Pd/mSiO2 catalyst. This study demonstrates a new role of GDY in constructing wettability matched catalysts for gas‐liquid‐solid tri‐phase reactions. [ABSTRACT FROM AUTHOR]

Owing to unique alkyne‐rich structure, graphdiyne (GDY) has been proven to be a superb support for anchoring metal catalysts. Herein we demonstrate a new role of GDY as the wettability modifier for enhanced hydrogenation catalysis. After loading a certain amount GDY nanospheres, the silica mesoporous channels become superaerophilic, which allows gaseous H2 to be directly stored inside, thus significantly increasing the H2 concentration around the palladium nanoparticles (NPs). At the same time, GDY nanospheres also alter the electronic structure of the Pd NPs via a strong d‐π interaction. Combining these two roles of GDY, allows the hydrogenation of benzaldehyde to proceed under ambient H2 pressure in water, with an impressive 4.3‐fold enhancement compared to the unmodified Pd/mSiO2 catalyst. This study demonstrates a new role of GDY in constructing wettability matched catalysts for gas‐liquid‐solid tri‐phase reactions. [ABSTRACT FROM AUTHOR]

Background: The purpose of this study was to characterize the pathophysiological changes of hydronephrosis caused by ureteral calculi obstruction in a new rabbit ureteral calculi model by implanting flowable resin.Methods: Forty New Zealand rabbits were randomly divided into two groups: the calculi group and the sham control group. In the calculi group (n = 20), rabbits were operated at left lower abdomen and the left ureter was exposed. Then flowable resin (flowable restorative dental materials) was injected into the left ureter using a 0.45 mm diameter intravenous infusion needle. Then light-cured for 40 s by means of a dental curing light to form calculi. In the sham control group, normal saline was injected into the ureter. Rabbits underwent X-ray and routine blood and urine tests preoperatively, as well as X-ray, CT, dissection, HE staining and routine blood and urine tests on 1, 3, 5 and 7 days postoperatively. Stone formation was assessed by X-ray and unenhanced CT scan after surgery. The pathophysiological changes were evaluated through dissection, HE staining and routine blood and urine tests.Results: Ureteral calculi models were successfully constructed in 17 rabbits. In calculi group, high-density shadows were observed in the left lower abdomen on postoperative day 1st, 3rd, 5th and 7th by X-ray and CT scan. Dissection found obstruction formation of the left ureters, dilatation of the renal pelvis and upper ureter during 7 days after surgery. The renal long-diameters of the left ureters increased only on the 1st postoperative day. HE staining found ureteral and kidney damage after surgery. In calculi group and sham group,the serum creatinine, urea nitrogen, white blood cells and urine red blood cells were raised at day 1 after surgery. However, the indicators returned to normal at day 3, 5, and 7.Conclusions: This is a stable, less complicated operation and cost-effective ureteral calculi model by implanting flowable resin. And this novel model may allow us to further understand the pathophysiology changes caused by ureteral calculi obstruction. [ABSTRACT FROM AUTHOR]

The dual-deck architecture with aligned upper and lower decks is considered a promising technology to meet the demand of increasing word-line (WL) layers of 3D NAND flash. However, the relevant reliability studies are still lacking for the dual-deck 3D NAND array. In this work, it is reported an abnormal program disturbance phenomena of the bottom WLs in the upper-deck, and the physical mechanisms were studied. According to experimental analysis and TCAD simulations, the un-programmed dummy WLs at the joint region can introduce excessive joint residual electrons in the channel before the program, resulting in insufficient channel self-boosting potential, which is responsible for the degraded program disturbance. Thus, a novel program scheme is proposed to alleviate the program disturbance, which has been validated by experiments. [ABSTRACT FROM AUTHOR]

Psoriasis is a chronic immune-mediated skin disease. Recent studies showed its pathogenesis involved circular RNA (circRNA). However, the role of circ_0060531 in psoriasis development and the behind mechanism remain to be explored. Psoriasis cell model was constructed by treating keratinocytes (HaCaT cells) using interleukin 22 (IL-22). Expression of circ_0060531, microRNA-330-5p (miR-330-5p) and GRB2 associated binder 1 (GAB1) was determined by quantitative real-time polymerase chain reaction. The functional effects of circ_0060531 on IL-22-caused cell injury were investigated by 3-(4,5-Dimethylthazol-2-yl)-2,5-diphenyltetrazolium bromide, 5-Ethynyl-29-deoxyuridine, wound-healing and enzyme-linked immunosorbent assays. Protein expression was analysed by Western blot. The interactions among circ_0060531, miR-330-5p and GAB1 were identified by dual-luciferase reporter or RNA immunoprecipitation assay. Circ_0060531 and GAB1 expression were significantly increased, while miR-330-5p was decreased in psoriatic skin biopsies and IL-22-stimulated HaCaT cells in comparison with controls. In function, circ_0060531 knockdown assuaged IL-22-induced cell proliferation, cell migration and inflammation. Besides, circ_0060531 acted as a miR-330-5p sponge, and regulated the processes of IL-22-treated HaCaT cells by binding to the miRNA. Under the treatment of IL-22, miR-330-5p mediated HaCaT cell damage by targeting GAB1. Importantly, circ_0060531 modulated GAB1 production by interacting with miR-330-5p. Circ_0060531 knockdown assuaged IL-22-induced keratinocyte dysfunction through miR-330-5p/GAB1 pathway, proving a novel target for the therapy of psoriasis. [ABSTRACT FROM AUTHOR]

Single atom catalysts (SACs) with metal1-Nx sites have shown promising activity and selectivity in direct catalytic oxidation of benzene to phenol. The reaction pathway is considered to be involving two steps, including a H2O2 molecule dissociated on the metal single site to form the (metal1-Nx)=O active site, and followed by the dissociation of another H2O2 on the other side of metal atom to form O=(metal1-Nx)=O intermediate center, which is active for the adsorption of benzene molecule via the formation of a C-O bond to form phenol. In this manuscript, we report a Cu SAC with nitrogen and oxygen dual-coordination (Cu1-N3O1 moiety) that doesn't need the first H2O2 activation process, as verified by both experimental and density function theory (DFT) calculations results. Compared with the counterpart nitrogen-coordinated Cu SAC (denoted as Cu1/NC), Cu SAC with nitrogen and oxygen dual-coordination (denoted as Cu1/NOC) exhibits 2.5 times higher turnover frequency (TOF) and 1.6 times higher utilization efficiency of H2O2. Particularly, the coordination number (CN) of Cu atom in Cu1/NOC maintains four even after H2O2 treatment and reaction. Combining DFT calculations, the dynamic evolution of single atomic Cu with nitrogen and oxygen dual-coordination in hydroxylation of benzene is proposed. These findings provide an efficient route to improve the catalytic performance through regulating the coordination environments of SACs and demonstrate a new reaction mechanism in hydroxylation of benzene to phenol reaction. [ABSTRACT FROM AUTHOR]

Background: Community-acquired Methicillin-resistant Staphylococcus aureus (CA-MRSA) is an emerging pathogen that leads to severe outcomes, especially in pediatric patients with multiple sites infection.Case Presentation: We report a case of multiple sites and life-threatening infection caused by CA-MRSA in a 6-year-old girl who manifested sepsis, myelitis, purulent arthritis, purulent meningitis, hydropericardium, pneumonia, and empyema. The girl exhibited good response to the combination therapy of linezolid and rifampicin after treatment failure of vancomycin with maximum dose due to its serum concentration unable to reach therapeutic goal. We performed pleural effusion and hydropericardium effusion drainage and treated left lower limb infection using interdisciplinary approaches.Conclusion: This case highlights the need to be aware of CA-MRSA infection, which requires accurate diagnosis, identification of infected sites, appropriate antibiotic treatment, and surgical debridement. [ABSTRACT FROM AUTHOR]

The repeaters in cascade structure can be used to suppress the cross coupling between the nonadjacent repeaters in multiload inductive power transfer (ML-IPT) system. In this article, the cascade repeaters used in the ML-IPT system work both as power transmitters and load carriers. Based on the cascade scheme, the system models of two ML-IPT systems with single-excitation (SE) scheme for different load position are established and high-order compensations are designed to achieve constant load currents. Referring to the topologies of the two ML-IPT systems with SE scheme, a bilateral-excitation (BE) scheme is further proposed to improve output currents and system reliability, and the comparison of the conventional scheme and the proposed scheme is also presented. Finally, a prototype of ML-IPT system with the proposed BE scheme is built, where the maximum deviation of the output currents is less than 13% and the maximum power transfer efficiency reaches 89.2% for an eight-load case. [ABSTRACT FROM AUTHOR]

Despite their limitations, unfractionated heparin (UFH) and bivalirudin remain standard-of-care parenteral anticoagulants for percutaneous coronary intervention (PCI). We discovered novel direct thrombin inhibitors (DTIs) from tick salivary transcriptomes and optimised their pharmacologic activity. The most potent, ultravariegin, inhibits thrombin with a Ki of 4.0 pM, 445-fold better than bivalirudin. Unexpectedly, despite their greater antithrombotic effect, variegin/ultravariegin demonstrated less bleeding, achieving a 3-to-7-fold wider therapeutic index in rodent thrombosis and bleeding models. When used in combination with aspirin and ticagrelor in a porcine model, variegin/ultravariegin reduced stent thrombosis compared with antiplatelet therapy alone but achieved a 5-to-7-fold lower bleeding time than UFH/bivalirudin. Moreover, two antibodies screened from a naïve human antibody library effectively reversed the anticoagulant activity of ultravariegin, demonstrating proof-of-principle for antidote reversal. Variegin and ultravariegin are promising translational candidates for next-generation DTIs that may reduce peri-PCI bleeding in the presence of antiplatelet therapy. Bleeding complications limits the use of effective antithrombotics therapeutics. Here, the authors developed next-generation direct thrombin inhibitors with low bleeding risks as safe peri-percutaneous coronary intervention anticoagulants when used in combination with antiplatelets. [ABSTRACT FROM AUTHOR]

The carbon–carbon bond cleavage of 1,2-diols is an important chemical transformation. Although traditional stoichiometric and catalytic oxidation methods have been widely used for this transformation, an efficient and valuable method should be further explored from the views of reusable catalysts, less waste, and convenient procedures. Herein an inorganic-ligand supported iron catalyst (NH4)3[FeMo6O18(OH)6]·7H2O was described as a heterogeneous molecular catalyst in acetic acid for this transformation in which hydrogen peroxide was used as the terminal oxidant. Under the optimized reaction conditions, carbon–carbon bond cleavage of 1,2-diols could be achieved in almost all cases and carboxylic acids or ketones could be afforded with a high conversion rate and high selectivity. Furthermore, the catalytic system was used efficiently to degrade renewable biomass oleic acid. Mechanistic insights based on the observation of the possible intermediates and control experiments are presented. [ABSTRACT FROM AUTHOR]

NAZ2329 is the first cell-permeable small molecular allosteric inhibitor of protein tyrosine phosphatase receptor–type Z (PTPRZ), which was considered as a new potential molecular target for drug development in glioblastoma. A facile and high-efficient synthesis of NAZ2329 was developed by a straight-forward strategy starting from key ethoxy benzyl bromide intermediate obtained from commercially available 4-(trifluoromethyl)phenol. [ABSTRACT FROM AUTHOR]

Engineered cartilage has several applications in treating cartilage ossification, however, its use is restricted clinically. We explored the feasibility of engineered cartilage in constructing tissues using gelatin/curcumin nano-fiber membranes as scaffolds in subcutaneous models. We constructed cartilage with gelatin nano-fiber membrane (control group) and gelatin/curcumin nano-fiber membrane (experimental group) as scaffolds. After the material was implanted into the back of BALB/c mice, gross view observation was performed. Histological examination was performed 3 and 12 weeks after implantation in vivo, and cartilage formation at different time points was compared. Gross observation showed that compared to the control group, the vascularization of nearby tissues in the experimental group was significantly inhibited. The Scanning electron microscope observation showed that the chondrocytes in both groups adhered well. The growth curve of the chondrocytes showed that curcumin had no significant effect on cell growth. Histological observation showed that the cell-material complexes in both groups had cartilage lacuna formation at 3 and 12 weeks. However, compared with that of the control group, the experimental group showed obvious absorption and thicker cartilage matrix with more homogenization. Gelatin/curcumin scaffolds were successfully used to construct engineered cartilage tissues in subcutaneous animal models. Our findings demonstrate that curcumin-loaded scaffolds have great clinical applications. [ABSTRACT FROM AUTHOR]

Objectives: To develop a population pharmacokinetic model of meropenem in children with sepsis receiving extracorporeal life support (ECLS) and optimize the dosage regimen based on investigating the probability of target attainment (PTA). Methods: The children with sepsis were prospectively enrolled in a pediatric intensive care unit from January 2018 to December 2019. The concentration-time data were fitted using nonlinear mixed effect model approach by NONMEM program. The stochastic simulation considering various scenarios based on proposed population pharmacokinetics model were conducted, and the PTAs were calculated to optimize the dosage regimens. Results: A total of 25 children with sepsis were enrolled, of whom13 received ECMO, 9 received CRRT, and 4 received ECMO combined with CRRT. 12 children received a two-step 3-h infusion and 13 children received 1-h infusion. Bodyweight and creatinine clearance had significant impacts on the PK parameters. ECMO intervention was not related to the PK properties. If 100%T > MIC was chosen as target, children receiving 40 mg/kg q8h over a 3 h-infusion only reached the PTA up to 77.4%. If bacteria with MIC 2 mg/L were to be treated with meropenem and the PTA target was 50%T > MIC, a dose of 40 mg/kg q8h for 1 h infusion would be necessary. Conclusions: The PK properties of meropenem in septic children receiving extracorporeal life support were best described. We recommended the opitimized dosing regimens for septic children receiving ECLS depending on the PTA of PK target 50%T > MIC and 100%T > MIC, for children with sepsis during ECLS with different body weight, estimated creatinine clearance (eCRCL) and MIC of bacteria. [ABSTRACT FROM AUTHOR]

Bloodstream infection is a life-threatening complication in critically ill patients. Multi-drug resistant bacteria or fungi may increase the risk of invasive infections in hospitalized children and are difficult to treat in intensive care units. The purpose of this study was to use metagenomic next-generation sequencing (mNGS) to understand the bloodstream microbiomes of children with suspected sepsis in a pediatric intensive care unit (PICU). mNGS were performed on microbial cell-free nucleic acid from 34 children admitted to PICU, and potentially pathogenic microbes were identified. The associations of serological inflammation indicators, lymphocyte subpopulations, and other clinical phenotypes were also examined. mNGS of blood samples from children in PICU revealed potential eukaryotic microbial pathogens. The abundance of Pneumocystis jirovecii was positively correlated with a decrease in total white blood cell count and immunodeficiency. Hospital-acquired pneumonia patients showed a significant increase in blood bacterial species richness compared with community-acquired pneumonia children. The abundance of bloodstream bacteria was positively correlated with serum procalcitonin level. Microbial genome sequences from potential pathogens were detected in the bloodstream of children with suspected sepsis in PICU, suggesting the presence of bloodstream infections in these children. [ABSTRACT FROM AUTHOR]

1T-phase molybdenum disulphide (MoS2) is considered to be one of the most promising candidates to substitute noble metals in the hydrogen evolution reaction (HER). Fabricating single layer 1T-phase MoS2 with abundant sulfur vacancies as well as good stability to further enhance catalytic performance remains a challenge. Herein, we develop an efficient in situ TMA cation ((CH3)4N+) intercalation method to produce 1T-phase MoS2. The obtained MoS2 nanosheets comprise about 81% 1T-phase and abundant sulfur vacancies (>12%), as verified by aberration-corrected high-angle annular dark field scanning transmission electron microscopy (AC-HAADF-STEM), X-ray photoelectron spectroscopy (XPS), X-ray absorption fine structure (XAFS), etc. In addition, the surface of as-prepared ST-MoS2 is negatively charged, making it very stable in aqueous solution. The as-prepared ST-MoS2 exhibits excellent electrochemical HER performance with a low over-potential and a small Tafel slope as well as superior cycling stability. Furthermore, it is an excellent co-catalyst when coupled with CdS nanowires to form a CdS/ST-MoS2 heterojunction photocatalyst for the HER under visible light irradiation. It achieves a maximum H2 production rate and apparent quantum efficiency (AQE) of approximately 1187 μmol mg−1 h−1 and 73.4%, respectively, which are significantly higher than those of reported CdS/MoS2 photocatalysts. [ABSTRACT FROM AUTHOR]

Low-bandgap conjugated polymers with photocurrent response to near-infrared (NIR) wavelength are the basis of optoelectronic technologies such as semitransparent organic photovoltaics (STOPVs), NIR polymer photodetectors (PPDs), ambipolar field-effect transistors (FETs), photoacoustic (PA) imaging and photothermal therapy (PTT). Compared with inorganic-based photodetectors, PPDs, based on low-bandgap conjugated polymers with the chemical structures easy to be tuned, can achieve a wide range of spectral responses. On the other hand, this kind of polymers can enhance the short-circuit current (JSC) in TOPV by extending the absorption spectrum range. In this review, our focus will be on the development of low-bandgap conjugated polymers extending over 1000 nm and the performance of PPDs or OPVs. The perspective article summarizes the design strategy of such polymers, the derivation process of the useful heterocycles, absorption, bandgap, and energy levels. Thereafter, we will draw attention to the challenges and the possible research direction in the future to provide readers with a deeper understanding of the design of NIR polymers. [ABSTRACT FROM AUTHOR]

Summary: What is known and Objective: Meropenem, a broad‐spectrum carbapenem, is frequently used to treat severe bacterial infections in critically ill children. Recommendations for meropenem doses in adult infections are available; however, few studies have been published regarding the use of meropenem in children with sepsis, especially in those receiving continuous renal replacement therapy (CRRT) and extracorporeal membrane oxygenation (ECMO). We aimed to investigate the pharmacokinetic (PK) parameters of meropenem in children with sepsis receiving extracorporeal life support (ECLS). Methods: This was a prospective observational clinical study of children with sepsis receiving ECMO or CRRT in the paediatric intensive care unit (PICU) of a children's hospital. The enrolled children received 20 mg/kg meropenem infusion over 1 hour, every 8 hours, and were grouped into children receiving ECMO, children receiving CRRT and children receiving neither ECMO nor CRRT. Plasma meropenem concentrations were determined using a validated high‐performance liquid chromatography‐tandem mass spectrometry (HPLC‐MS/MS). The key PK parameters were determined using the non‐compartmental approach. Results and discussion: Twenty‐seven patients were finally enrolled. The eCLCR of the CRRT group was lower than that of the ECMO group. The values of elimination half‐life (t1/2), area under the plasma concentration‐time curve (AUCtau), area under the plasma concentration‐time curve from time zero to infinity (AUC0‐∞), and total clearance (CL) in the ECMO group were not different from those of the other groups (all p > 0.05). However, the AUCtau (p = 0.0137) and AUC0‐∞ (p = 0.0234) significantly decreased after filtration through a hemofiltration membrane in patients receiving CRRT. What is new and Conclusion: No significant alterations in the PK parameters of meropenem occurred in children with sepsis administered ECMO and/or CRRT. Further investigations including PK modelling could provide evidence for appropriate meropenem dosing regimens during ECLS administration. [ABSTRACT FROM AUTHOR]