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In the face of climate change, cropping systems need to achieve a high performance, providing food and feed and adapting to variable environmental conditions. Diversification of cropping systems can support ecosystem services and associated biodiversity, but there is little evidence on which temporal field arrangement affects the performance of crop yields (productivity and stability), partly due to a lack of long-term data and appropriate indicators. The objectives of this study were to quantify the effect of cropping system diversification on yield stability, environmental adaptability, and the probability of diversified systems to outperform less diverse cereal-based systems in Europe. Spring and winter cereal yields were analyzed from long-term field experiments from Sweden, Scotland, and France. We investigated diversification through (i) introduction of perennial leys, (ii) increasing the proportion of ley in the rotation, (iii) varying the order in which crops are positioned in the rotation, (iv) introduction of grain legumes, and (v) introduction of cover crops. The results showed that cereal crops within cropping systems incorporating perennial leys outperformed systems without leys in 60–94% of the comparisons with higher probabilities at low fertilizer intensities. The yield stability of oat did not differ, but mean yields were 33% higher, when grown directly after the ley compared to oat grown two years later in the crop sequence under similar management. Durum wheat grown in a cropping system with grain legumes had higher yields in lower-yielding environmental conditions compared to rotations without legumes. Diversification with cover crops did not significantly affect yield stability. We conclude that diverse cropping systems can increase cereal productivity and environmental adaptability and are more likely to outperform less diverse systems especially when introducing perennial forage legumes into arable systems. Effects of diversification on cereal yield stability were inconsistent indicating that higher productivity is achievable without reducing yield variability. These novel findings can support the design of more diverse and high-performing cropping systems. [ABSTRACT FROM AUTHOR]

There is a growing interest among farmers and consumers in increasing production and consumption of grain legumes in Sweden. This requires better knowledge among farmers and advisors about suitable species, varieties and management practices for different conditions. Since cultivar suitability and management practices are highly site-specific, farmers need to gain their own experience of new crops and practices. This paper describes a farmer–researcher collaboration in which cultivation of grain legumes for food was investigated in on-farm experiments designed, managed and evaluated jointly by a group of farmers and researchers. Farmers tested innovative practices using within-field species diversity, comparative performance of varieties and methods for weed control. Post-harvest steps such as cleaning and selling the crops were considered by farmers to be integral components of the experiments. The process generated different types of knowledge, including practical knowledge on crop management, strategic knowledge on economic sustainability and knowledge about joint learning through collaboration. The on-farm experiments combined advantages of 'pure' farmer experiments (i.e., context specificity) and 'pure' researcher experiments (i.e., scientific inquiry), facilitating deeper analysis and understanding of outcomes. This enabled efficient knowledge building, adoption of new crops and innovative practices and stimulated further experimentation. The outcomes of this study are that farmer–researcher collaborations using on-farm experiments can stimulate collective learning by stimulating the exchange between participants and combining complementary perspectives throughout the experimentation process. The study also provides recommendations for facilitating on-farm experiments in future work, for instance using collective settings to evaluate the results. [ABSTRACT FROM AUTHOR]

Diversifying cropping systems by increasing the number of cash and cover crops in crop rotation plays an important role in improving resource use efficiency and in promoting synergy between ecosystem processes. The objective of this study was to understand how the combination of crop diversification practices influences the performance of arable crop sequences in terms of crop grain yield, crop and weed biomass, and nitrogen acquisition in a temperate climate. Two field experiments were carried out. The first was a 3-year crop sequence with cereal or grain legume as the first crops, with and without undersown forage legumes and forage legume-grass crops, followed by a cereal crop. The second experiment was a 2-year crop sequence with cereal or legume as the first crops, a legume cover crop, and a subsequent cereal crop. For the first time, crop diversification practices were combined to identify plant-plant interactions in spatial and temporal scales. The results partly confirm the positive effect of diversifying cereal-based cropping systems by including grain legumes and cover crops in the crop sequence. Legume cover crops had a positive effect on subsequent cereal grain yield in one of the experiments. Using faba beans as the first crop in the crop sequence had both a positive and no effect on crop biomass and N acquisition of the subsequent cereal. In cover crops composed of a forage legume-grass mixture, the grass biomass and N acquisition were consistently increased after the grain legume, compared to the cereal-preceding crop. However, differences in the proportion of legume to grass in mixture did not influence crop yield or N acquisition in the subsequent cereal. In conclusion, these results support that increased crop diversity across spatial and temporal scales can contribute to resource-efficient production and enhance the delivery of services, contributing to more sustainable cropping systems. [ABSTRACT FROM AUTHOR]

Modern agriculture needs to develop transition pathways toward agroecological, resilient and sustainable farming systems. One key pathway for such agroecological intensification is the diversification of cropping systems using intercropping and notably cereal-grain legume mixtures. Such mixtures or intercrops have the potential to increase and stabilize yields and improve cereal grain protein concentration in comparison to sole crops. Species mixtures are complex and the 4C approach is both a pedagogical and scientific way to represent the combination of four joint effects of Competition, Complementarity, Cooperation, and Compensation as processes or effects occurring simultaneously and dynamically between species over the whole cropping cycle. Competition is when plants have fairly similar requirements for abiotic resources in space and time, the result of all processes that occur when one species has a greater ability to use limiting resources (e.g., nutrients, water, space, light) than others. Complementarity is when plants grown together have different requirements for abiotic resources in space, time or form. Cooperation is when the modification of the environment by one species is beneficial to the other(s). Compensation is when the failure of one species is compensated by the other(s) because they differ in their sensitivity to abiotic stress. The 4C approach allows to assess the performance of arable intercropping versus classical sole cropping through understanding the use of abiotic resources. [ABSTRACT FROM AUTHOR]

Summary: Fabeae legumes such as pea and faba bean form symbiotic nodules with a large diversity of soil Rhizobium leguminosarum symbiovar viciae (Rlv) bacteria. However, bacteria competitive to form root nodules (CFN) are generally not the most efficient to fix dinitrogen, resulting in a decrease in legume crop yields. Here, we investigate differential selection by host plants on the diversity of Rlv.A large collection of Rlv was collected by nodule trapping with pea and faba bean from soils at five European sites. Representative genomes were sequenced. In parallel, diversity and abundance of Rlv were estimated directly in these soils using metabarcoding. The CFN of isolates was measured with both legume hosts. Pea/faba bean CFN were associated to Rlv genomic regions.Variations of bacterial pea and/or faba bean CFN explained the differential abundance of Rlv genotypes in pea and faba bean nodules. No evidence was found for genetic association between CFN and variations in the core genome, but variations in specific regions of the nod locus, as well as in other plasmid loci, were associated with differences in CFN.These findings shed light on the genetic control of CFN in Rlv and emphasise the importance of host plants in controlling Rhizobium diversity. [ABSTRACT FROM AUTHOR]

The Western diet is characterized by high meat consumption, which negatively affects the environment and human health. Transitioning toward eating more plant-based products in Western societies has been identified as a key instrument to tackle these problems. However, one potential concern is that radically reducing meat in the current diet might lead to deficiencies in nutritional intake. In this paper, we explore a scenario in which meat consumption in Sweden is reduced by 50% and replaced by domestically grown grain legumes. We quantify and discuss the implications for nutritional intake on population level, consequences for agricultural production systems and environmental performance. The reduction in meat consumption is assumed to come primarily from a decrease in imported meat. We use data representing current Swedish conditions including the Swedish dietary survey, the Swedish food composition database, Statistics Sweden and existing life cycle assessments for different food items. At population level, average daily intake of energy and most macro- and micro-nutrients would be maintained within the Nordic Nutrition Recommendations after the proposed transition (e.g., for protein, fat, zinc, vitamin B12 and total iron). The transition would also provide a considerable increase in dietary fiber and some increase in folate intake, which are currently below the recommended levels. The transition scenario would increase total area of grain legume cultivation from 2.2% (current level) to 3.2% of Swedish arable land and is considered technically feasible. The climate impact of the average Swedish diet would be reduced by 20% and the land use requirement by 23%. There would be a net surplus of approximately 21,500 ha that could be used for bioenergy production, crop production for export, nature conservation, etc. Implementation of this scenario faces challenges, such as lack of suitable varieties for varying conditions, lack of processing facilities to supply functional legume-based ingredients to food industries and low consumer awareness about the benefits of eating grain legumes. In sum, joint efforts from multiple actors are needed to stimulate a decrease in meat consumption and to increase cultivation and use of domestically grown grain legumes. [ABSTRACT FROM AUTHOR]

Planetary boundaries for terrestrial inputs of reactive nitrogen (Nr) are transgressed and reducing the input of new Nr and its environmental impacts are major global challenges. Grain legumes fix dinitrogen (N2) in symbiosis with soil bacteria and use soil N sources, but often less efficient than cereals. Intercropping grain legumes with cereals may be a means of increasing use efficiency of soil N. Here, we estimate the global sole cropped grain legume acquisition of N from soil to approximately 14.2 Tg N year−1, which corresponds to one-third of the global synthetic fertilizer N use (109 Tg N year−1) for all crops, assuming that grain legumes recover on average 40% of the fertilizer N. Published data from grain legume-cereal intercrop experiments, employing stable 15N isotope methods, have shown that due to competitive interactions and complementary N acquisition in intercrops, the cereals recover a more than proportional share of the soil N sources. As a consequence, the intercropped legume derives more of its N from the atmosphere, compared with when it is grown as legume sole crop. We estimated that the increased N use efficiency in intercropping can reduce the requirements for fossil-based fertilizer N by about 26% on a global scale. In addition, our estimates indicate that if all current grain legume sole crops would instead be intercropped with cereals, a potential net land saving would be achieved, when also replacing part of the current cereal sole crop area with intercropping. Intercropping has additional potential advantages such as increased yield stability and yield per unit area, reduced pest problems and reduced requirements for agrochemicals, while stimulating biodiversity. It is concluded that crop diversification by intercropping has the potential to reduce global requirements for synthetic fertilizer N and consequently support the development of more sustainable cropping systems. [ABSTRACT FROM AUTHOR]

Recirculation of nitrogen (N) from crop residue and green-manure biomass resources may reduce the need to add new reactive N to maintain crop yield and quality. The aim of this study was to determine how different strategies for recycling residual and green-manure biomass influence yield and N concentration of the edible parts of food crops in a stockless organic cropping system. For this purpose, three biomass distribution treatments were investigated in a field experiment, based on a cropping system designed to produce both high-quality food crops and biomass resources from crop residues, cover crops and a green-manure ley. The three treatments, applied at the cropping system level, were: (1) incorporating the aboveground biomass resources in situ (IS); (2) harvesting, ensiling and redistributing the same biomass resources to the non-legume crops (biomass redistribution , BR); and (3) harvesting, ensiling and using the biomass resources as substrate for production of bio-methane via anaerobic digestion (AD) followed by distribution of the digestate as bio-fertilizer to the non-legume crops. The redistribution of ensiled (BR) and digested (AD) biomass did not increase the yield of the edible parts in winter rye (Secale cereal L.), white cabbage (Brassica oleracea L.) or red beet (Beta vulgaris L.) compared with leaving the biomass on the ground at harvest (IS). The BR treatment increased the yield of lentil intercropped with oat, compared with IS treatment in one of the two studied years. The total biomass yield of the cover crop following winter rye was significantly higher in the BR treatment than in IS in both years. The legume proportion in the green-manure ley was significantly higher in the AD and BR treatments as compared with IS in one of the experimental years. This study showed that strategic biomass redistribution has the potential to enhance biomass productivity while maintaining food crop yields, thereby enhancing whole system productivity. Biomass redistribution systems both with and without biogas digestion offer a new strategy for the development of multifunctional arable cropping systems that rely on internal nutrient cycling. [ABSTRACT FROM AUTHOR]

Aims: A better understanding of how plant growth, N nutrition and symbiotic nitrogen fixation (SNF) are influenced by soil inorganic N availability, for a wide range of legume species, is crucial to optimise legume productivity, N2 fixation, while limiting environmental risks such as N leaching.Methods: A comparative analysis was performed for ten legume crops, grown in a field experiment and supplied with four N fertiliser rates. Dry matter, N concentration and SNF were measured. In parallel, root elongation rates were studied in a greenhouse experiment.Results: For most species, N fertilisation had little effect on plant growth and N accumulation. SNF was reduced by soil inorganic N available at sowing but with large differences in the magnitude of the response among species. The response varied according to plant N requirements for growth and plant ability to retrieve inorganic N. Accordingly, root lateral expansion rate measured in RhizoTubes was highly correlated with plant ability to retrieve inorganic N measured in the field experiment.Conclusion: Combining SNF response to soil inorganic N, shoot N and plant ability to retrieve inorganic N, allowed a robust evaluation of differential response to soil inorganic N among a wide range of legume species. [ABSTRACT FROM AUTHOR]

A major future challenge in agriculture is to reduce the use of new reactive nitrogen (N) while maintaining or increasing productivity without causing a negative N balance in cropping systems. We investigated if strategic management of internal biomass N resources (green manure ley, crop residues and cover crops) within an organic crop rotation of six main crops, could maintain the N balance. Two years of measurements in the field experiment in southern Sweden were used to compare three biomass management strategies: anaerobic digestion of ensiled biomass and application of the digestate to the non-legume crops (AD), biomass redistribution as silage to non-legume crops (BR), and leaving the biomass in situ (IS). Neither aboveground crop N content from soil, nor the proportion of N derived from N2 fixation in legumes were influenced by biomass management treatment. On the other hand, the allocation of N-rich silage and digestate to non-legume crops resulted in higher N2 fixation in AD and BR (57 and 58 kg ha−1 year−1), compared to IS (33 kg ha−1 year−1) in the second study year. The N balance ranged between − 9.9 and 24 kg N ha−1, with more positive budgets in AD and BR than in IS. The storage of biomass for reallocation in spring led to an increasing accumulation of N in the BR and AD systems from one year to another. These strategies also provide an opportunity to supply the crop with the N when most needed, thereby potentially decreasing the risk of N losses during winter. [ABSTRACT FROM AUTHOR]

Abstract: Background: Legume biological nitrogen (N2)-fixation is stimulated by neighbouring non-fixing species, but studies of legume N2-fixation in temporary grasslands including non-leguminous forage herb (forb) species are rare.Methodology: We investigated N2-fixation, N yield, and total herbage production in a range of species mixtures consisting of three forb species—chicory (Cichorium intybus L.), ribwort plantain (Plantago lanceolata L.), and caraway (Carum carvi L.)—mixed into a traditional red clover (Trifolium pratense L.) and perennial ryegrass (Lolium perenne L.) mixture at two fertilisation levels.Results: The percentage of red clover N derived from the atmosphere (%Ndfa) was higher in mixtures containing non-legumes than in pure stand but, did not increase with inclusion of forbs. On a whole-seasonal basis, red clover in mixtures derived 90% or more of its N from fixation even when fertilised with 216 kg total N ha−1. Forbs, in particular chicory, reduced the amount of N2-fixation and total N yields by affecting the red clover proportion in the harvested biomass.Conclusions: Generally, inclusion of forbs in red clover-ryegrass mixtures had no negative effect on total herbage production and percentage of legume N2-fixation. However, to maintain a high total N and N2-fixation yields, mixtures should not include a high seeding proportion of chicory. [ABSTRACT FROM AUTHOR]

Background and aims: While N-fixation in diversified grasslands including forage legumes and non-legumes has been widely studied, N-fixation in swards containing only forage legumes remains unclear. In this study, we investigated N-fixation in pure stands and mixtures of three forage legumes. Methodology: N-fixation, dry matter (DM) and nitrogen (N) yields were quantified in a field experiment for red clover ( Trifolium pratense L.), white clover ( Trifolium repens L.) and lucerne ( Medicago sativa L.) pure stands and mixtures using the isotope dilution method. Results: All three forage legume species derived most (around 85%) of their N from atmospheric N-fixation (%Ndfa). However, no positive effect of species diversity was found in any of the mixtures. Species composition of the forage legume mixtures affected the amount of N from N-fixation by affecting DM production and N accumulation of the species, where the seasonal amount of N-fixation ranged from 370 to 500 kg N ha; which was highest in the presence of red clover. Conclusions: We found that mixtures of the three forage legumes were highly productive, but did not show positive advantages compared to the red clover pure stands in terms of DM, N yield and %Ndfa. [ABSTRACT FROM AUTHOR]

Background and aims: There is substantial evidence that legume-derived Nitrogen (N) is transferred to neighboring non-legumes in grassland mixtures. However, there is sparse information about how deep rooted non-legume forage herbs (forbs) influence N transfer in multi-species grasslands. Methodology: Red clover ( Trifolium pretense L.) was grown together with perennial ryegrass ( Lolium perenne L.) and one of three forb species: chicory ( Cichorium intybus L.), ribwort plantain ( Plantago lanceolata L.) or caraway ( Carum carvi L.) in a field experiment. During the first year after the establishment, red clover leaves were labeled with N-urea to determine the N transfer from red clover to companion ryegrass and forbs. Results: On an annual basis, up to 15 % of red clover N was transferred to the companion ryegrass and forbs, but predominantly to the grass. The forb species did not differ in their ability to take up clover N, but biomass production and soil N acquisition was higher in chicory and plantain than in caraway. Conclusions: Grass relied to a great extent on clover N, whereas forbs relied on soil N. Soil N-enrichment indicated that N transfer occurred in the upper soil layers and that a dependence on clover-derived N did not necessarily give grass a growth advantage. [ABSTRACT FROM AUTHOR]

Multifunctional agriculture provides noncommodity functions and services along with food, feed and bioenergy feedstocks, for example by preserving or promoting biodiversity, improving soil fertility, mitigating climate change and environmental degradation, and contributing to the socio-economic viability of rural areas. Producing biomass for bioenergy from low-input perennial species mixtures on marginal land has the potential to support biodiversity and soil carbon sequestration in synergy with greenhouse gas mitigation. We compared biomass production in species-rich mixtures of perennial grasses, legumes and forbs with pure-stand grasses and relatively species-poor mixtures under different nitrogen fertilization regimes. Field experiments were performed on different types of marginal land, that is agricultural field margins and land with poor soil fertility, at four sites in southernmost and western Sweden. Biomass production was measured for three years in perennial grasses grown as pure stands, in legume-grass mixtures, and legume-grass-forb mixtures across a species richness gradient. In unfertilized species-rich mixtures, average biomass yields per experimental site and year were in the range from 3 to 9 metric ton DM ha−1 yr−1. While the most productive pure-stand grasses fertilized with 60-120 kg N ha−1 yr−1 often produced higher biomass yields than unfertilized mixtures, these differences were generally smaller than the variations between years and sites. Calculations of climate impact using the harvested biomass for conversion to biogas as vehicle fuel showed that the average greenhouse gas emissions per energy unit were about 50% lower in unfertilized systems than in treatments fertilized with 100-120 kg N ha−1 yr−1. Our findings thereby show that unfertilized species-rich perennial plant mixtures on marginal land provide resource-efficient biomass production and contribute to the mitigation of climate change. Perennial species mixtures managed with low inputs thus promote synergies between productivity and biodiversity in the perspective of climate-smart and multifunctional biomass production. [ABSTRACT FROM AUTHOR]

Below-ground niche complementarity in legume-cereal intercrops may improve resource use efficiency and root adaptability to environmental constraints. However, the effect of water limitation on legume rooting and nodulation patterns in intercropping is poorly understood. To advance our knowledge of mechanisms involved in water-limitation response, faba bean ( Vicia faba L.) and wheat ( Triticum aestivum L.) were grown as mono- and intercrops in soil-filled plexiglass rhizoboxes under water sufficiency (80% of water-holding capacity) and water limitation (30% of water-holding capacity). We examined whether intercropping facilitates below-ground niche complementarity under water limitation via interspecific root stratification coupled with modified nodulation patterns. While no significant treatment effects were measured in intercropped wheat growth parameters, water limitation induced a decrease in shoot and root biomass of monocropped wheat. Likewise, shoot biomass and height, and root length of monocropped faba bean significantly decreased under water limitation. Conversely, water limitation stimulated root biomass of intercropped faba bean in the lower soil layer (15-30 cm soil depth). Similarly, total nodule number of faba bean roots as well as nodule number in the lower soil layer increased under intercropping regardless of water availability. Under water limitation, intercropping also led to a significant increased nodule biomass (48%) in the lower soil layer as compared to monocropping. The enhanced nodulation in the lower soil layer and the associated increase in root and shoot growth provides evidence for a shift in niche occupancy when intercropped with wheat, which improves water-limited faba bean performance. [ABSTRACT FROM AUTHOR]

Despite the agronomical and environmental advantages of the cultivation of legumes, their production is limited by various environmental constraints such as water or nutrient limitation, frost or heat stress and soil salinity, which may be the result of pedoclimatic conditions, intensive use of agricultural lands, decline in soil fertility and environmental degradation. The development of more sustainable agroecosystems that are resilient to environmental constraints will therefore require better understanding of the key mechanisms underlying plant tolerance to abiotic constraints. This review provides highlights of legume tolerance to abiotic constraints with a focus on soil nutrient deficiencies, drought, and salinity. More specifically, recent advances in the physiological and molecular levels of the adaptation of grain and forage legumes to abiotic constraints are discussed. Such adaptation involves complex multigene controlled-traits which also involve multiple sub-traits that are likely regulated under the control of a number of candidate genes. This multi-genetic control of tolerance traits might also be multifunctional, with extended action in response to a number of abiotic constraints. Thus, concrete efforts are required to breed for multifunctional candidate genes in order to boost plant stability under various abiotic constraints. [ABSTRACT FROM AUTHOR]

Legume N2fixation is a critical plant-mediated nutrient pathway highly dependent on site and management conditions, yet little is known of annual crop N2fixation associated with established trees in tree-based intercropping systems. Our objective was to investigate the performance and N acquisition strategies of soybean in a tree-based intercropping system with four tree species (generaJuglans, Fraxinus, Acer, andPopulus) at two distances from the tree row (1- and 4-m tree-crop interface) in comparison to monocropped soybean. Soil nitrification rates were inversely related to soybean nodule production at the 1-m tree-crop interface (r = −0.64;p= 0.0260). However, regardless of inhibited nodule production, soybeans at the 1-m tree-crop interface were fixing N at high rates as compared to monocropped soybeans, possibly stimulated by belowground interspecies competition; nitrogen derived from atmosphere (Ndfa) in soybeans was highest when intercropped with spruce (75% Ndfa) and poplar (65% Ndfa) at the 1- and 4-m tree-crop interface, respectively. We show a shift in crop N acquisition strategies, as modified by tree species and distance. We suggest that an often overlooked nutrient pathway, nitrate deposition via tree canopy wash, may be an unaccounted abiotic factor influencing nodule formation of associated legume crops. [ABSTRACT FROM AUTHOR]

Background and aims: Transfer of fixed N from legumes to non-legume reference plants may alter the N signature of the reference plant as compared to the soil N available to the legume. This study investigates how N transfer influences the result of N-based N fixation measurements. Methods: We labelled either legumes or non-legumes with N and performed detailed analyses of N enrichment in mixed plant communities in the field. The results were used in a conceptual model comparing how different N transfer scenarios influenced the N signatures of legumes and reference plants, and how the resulting N fixation estimate was influenced by using reference plants in pure stand or in mixture with the legume. Results: Based on isotopic signatures, N transfer was detected in all directions: from legume to legume, from legume to non-legume, from non-legume to legume, from non-legume to non-legume. In the scenario of multidirectional N transfer, N fixation was overestimated by using a reference plant in pure stand. Conclusions: Fixed N transferred to neighbouring reference plants modifies the N signature of the soil N available both to the reference plant and the N-fixing legume. This provides strong support for using reference plants growing in mixture with the legumes for reliable quantifications of N fixation. [ABSTRACT FROM AUTHOR]

This study is the first to investigate quantitative effects of plant community composition and diversity on N2 fixation in legumes. N2 fixation in three perennial Trifolium species grown in field plots with varied number of neighbouring species was evaluated with the 15N natural abundance method (two field sites, several growing seasons, no N addition) and the isotope dilution method (one site, one growing season, 5 g N m−2). The proportion of plant N derived from N2 fixation, pNdfa, was generally high, but the N addition decreased pNdfa, especially in species-poor communities. Also following N addition, the presence of grasses in species-rich communities increased pNdfa in T. hybridum and T. repens L., while legume abundance had the opposite effect. In T. repens, competition for light from grasses appeared to limit growth and thereby the amount of N2 fixed at the plant level, expressed as mg N2 fixed per sown seed . We conclude that the occurrence of diversity effects seems to be largely context dependent, with soil N availability being a major determinant, and that species composition and functional traits are more important than species richness regarding how neighbouring plant species influence N2 fixation in legumes. [ABSTRACT FROM AUTHOR]

Leys, used for grazing or production of forage to be conserved as silage or hay, are very important crops in northern areas. In order to measure the N2 fixation in leys of varying ages and during different parts of the season, detailed measurements were taken of yield, N2 fixation and the amounts of N remaining in the field after harvesting red clover ( Trifolium pratense L.)-grass leys at a site in northern Sweden, where they are generally harvested twice per growing season. Entire plants, including stubble and roots, were sampled at the time of first and second harvest and, in addition, at the end of the growing season in three neighbouring fields, carrying a first, a second and a third year ley, respectively. N2 fixation was measured by both 15N isotope dilution (ID) and 15N natural abundance (NA) methods. The proportion of clover dry matter (DM) in the stands increased from the first to the second harvest, but the grasses dominated throughout the entire season, especially below ground. The N concentrations, in both herbage and whole plants, were about twice as high in the clover as in the grasses. Seasonal variations in N concentrations were minor, and total N contents followed the same trends as DM. The clover acquired nearly all of its N from N2 fixation: the proportion of N in clover herbage derived from N2 fixation was often >0.8 throughout the season. The variations in the amounts of N2 fixed during the course of the season corresponded well to the seasonal changes in clover biomass. Amounts of fixed N2 allocated to clover herbage during the whole season were in the range 4 to 6 g N m−2 in this unusually rainy year. Calculations of daily N allocation rates to herbage showed that N uptake rates were similar, and high, in grasses during May–June and July–August, while N2 fixation rates in clover were about 10-fold as high in July–August as in May–June, reflecting the need for N in clover growth. The proportion of N remaining in clover stubble and roots after the first and second harvests was about 60 and 25%, respectively, while about 60% of the N in grasses remained in stubble and roots after both harvests. The considerable amounts of biomass and N that were left in field after harvesting red clover-grass leys are important for re-growth of the plants and provide substantial N fertilization for the next crop in the crop rotation. [ABSTRACT FROM AUTHOR]

When estimating N 2 fixation with the 15 N natural abundance method, the 15 N abundance in plants grown with N 2 in air as only N source (B), must be known in order to account for discrimination against 15 N during N 2 fixation. Trifolium hybridum L., T. pratense L. and T. repens L. were grown without N in the nutrient solution in a greenhouse. The aim was to establish B values in these species in symbioses with Scandinavian Rhizobium genotypes, and to test the hypothesis that B is related to N 2 fixation efficiency and plant age. Choice of Rhizobium strain significantly influenced B in shoots of T. hybridum and T. repens , and N content in all Trifolium species. B in shoots was partly correlated to amounts of N 2 fixed, while plant age only had a marginal effect. Soil suspension inoculum or single strain inocula gave very similar B in shoots of T. pratense . [ABSTRACT FROM AUTHOR]

We measured aboveground plant biomass and soil inorganic nitrogen pools in a biodiversity experiment in northern Sweden, with plant species richness ranging from 1 to 12 species. In general, biomass increased and nitrate pools decreased with increasing species richness. Transgressive overyielding of mixed plant communities compared to the most productive of the corresponding monocultures occurred in communities with and without legumes. N2-fixing legumes had a fertilizing function, while non-legumes had a N retaining function. Plant communities with only legumes had a positive correlation between biomass and soil nitrate content, whereas in plant communities without legumes they were negatively correlated. Both nitrate and ammonium soil pools in mixed non-legume communities were approximately equal to the lowest observed in the corresponding monocultures. In mixed legume/non-legume communities, no correlation was found for soil nitrate with either biomass or legume biomass as percentage of total biomass. The idea of complementarity among species in nitrogen acquisition was supported in both pure non-legume and mixed non-legume/legume communities. In the latter, however, facilitation through increased nitrogen availability and retention, was probably dominating. Our results suggest that diversity effects on biomass and soil N pools through resource use complementarity depend on the functional traits of species, especially N2 fixation or high productivity. [ABSTRACT FROM AUTHOR]

The development of future food systems will depend on normative decisions taken at different levels by policymakers and stakeholders. Scenario modeling is an adequate tool for assessing the implications of such decisions, but for an enlightened debate, it is important to make explicit and transparent how such value-based decisions affect modeling results. In a participatory approach working with five NGOs, we developed a future food vision for the Nordic countries (Denmark, Finland, Norway and Sweden) through an iterative process of defining the scenario, modeling, and revising the scenario, until a final future food vision was reached. The impacts on food production, land use, and greenhouse gas emissions, and the resulting diets in the food vision, were modeled using a mass flow model of the food system. The food vision formulated was an organic farming system where food is produced locally and livestock production is limited to "leftover streams," i.e., by-products from food production and forage from pastures and perennial grass/clover mixtures, thus limiting food-feed competition. Consumption of meat, especially non-ruminant meat, was substantially reduced compared with current consumption in the Nordic countries (− 81%). An estimated population of 37 million people could be supplied with the scenario diet, which uses 0.21 ha of arable land and causes greenhouse gas emissions of 0.48 tCO2e per diet and year. The novelty of this paper includes advancing modeling of sustainable food systems by using an iterative process for designing future food visions based on stakeholder values, which enables results from multidisciplinary modeling (including agronomy, environmental system analysis, animal and human nutrition) to be fed back into the decision-making process, providing an empirical basis for normative decisions and a science-based future vision of sustainable food systems. [ABSTRACT FROM AUTHOR]