Search

This article explores the use of omics data, such as genomic, transcriptomic, proteomic, and metabolomic data, in chemical grouping and read-across for chemical risk assessment. It explains that traditional approaches to chemical safety rely on in vivo toxicity data, but there is a growing recognition of the need for more mechanistically based approaches that do not involve animal testing. The article introduces non-experts to the concept of grouping and read-across, discusses the value of omics technologies in this context, presents case studies, and identifies challenges and next steps for incorporating omics data into chemical grouping. It also discusses the regulatory landscape and the need for standardized reporting templates. However, it acknowledges that there are limitations to chemical grouping, as structural similarity does not always predict similar biological responses. Overall, the article highlights the potential of omics data in chemical grouping and the need for further research and implementation in this area. [Extracted from the article]

DCOIT (4,5-Dichloro-2-octylisothiazol-3(2H)-one) is a widely used antifouling biocide that emerged after the ban on tributyltin. It has been immobilized in nanostructured silica (SiNC–DCOIT) to reduce its hazard in maritime coatings. This study aimed to compare the bioaccumulation, trophic transfer, and biomagnification of DCOIT in its soluble and nanostructured forms on mussels Mytilus galloprovincialis, using three different uptake routes: aqueous exposure (i.e., contaminated seawater), dietary exposure (i.e., microalgae Tetraselmis chuii as a contaminated food), and both contaminated food and seawater. DCOIT was determined on water and tissues after 1, 3, and 24 h of uptake and after 72 h of depuration. Briefly, mussels were able to rapidly uptake and metabolize DCOIT and SiNC–DCOIT. Both compounds were non-bioaccumulative, as their bioconcentration and bioaccumulation factor values were lower than 2000. However, the predator–prey biomagnification factors indicated that both forms could be transferred across the trophic web. Therefore, while our findings provide further insight into the environmental risk assessment of DCOIT and SiNC–DCOIT, they do not rule out the possibility of long-term DCOIT bioaccumulation, particularly in areas with constant DCOIT influx. Further studies are thus needed using larger experimental designs and under continuous exposure scenarios to increase the sustainability of the innovative nanomaterial. [ABSTRACT FROM AUTHOR]

The article discusses the problem of chemical pollution and the need for innovative and exponential approaches to address it. It highlights the interconnectedness of climate change, biodiversity loss, and pollution, and emphasizes the importance of finding solutions to each issue. The authors propose two approaches: the concept of "Green Swans," which refers to desirable solutions that are resilient and regenerative, and application-oriented approaches to scientific roadmapping, which focus on generating solution strategies and tools for decision-making. The article concludes by calling on environmental scientists to contribute to the development and operationalization of Green Swan ideas to counter chemical pollution. [Extracted from the article]

The synthetic hormone 17α ethinyl estradiol (EE2) is a molecule widely used in female contraceptives and recognized as a contaminant of attention (Watch List) in the European Union due to its high consumption, endocrine effects and occurrence in aquatic environments. Its main source of introduction is domestic sewage where it can be associated with other contaminants such as microplastics (MPs). Due to their characteristics, they can combine with each other and exacerbate their isolated effects on biota. This study evaluated the combined effects of microplastics (MPs) and 17α ethinylestradiol (EE2) on two tropical estuarine invertebrate species: Crassostrea gasar and Ucides cordatus. Polyethylene particles were spiked with EE2 and organisms were exposed to three treatments, categorized into three groups: control group (C), virgin microplastics (MPs), and spiked microplastics with EE2 (MPEs). All treatments were evaluated after 3 and 7 days of exposure. Oysters exhibited changes in phase 2 enzymes and the antioxidant system, oxidative stress in the gills, and reduced lysosomal membrane stability after exposure to MPs and MPEs. Crabs exposed to MPs and MPEs after seven days showed changes in phase 1 enzymes in the gills and changes in phases 1 and 2 enzymes in the hepatopancreas, such as disturbed cellular health. The combined effects of microplastics and EE2 increased the toxicity experienced by organisms, which may trigger effects at higher levels of biological organization, leading to ecological disturbances in tropical coastal ecosystems. [ABSTRACT FROM AUTHOR]

The Paraiba do Sul River Basin (PSRB) is a vital source of water resources in Brazil, providing water for human consumption, industry, agriculture, and hydroelectric energy generation. As part of one of the most developed areas of the country, in the Southeast of Brazil, the region is vulnerable to the impacts of climate change, with evidence of extreme events such as droughts and floods affecting the availability and quality of water. Hence, this study analyzes precipitation and streamflow rates data from the PSRB between 1939–2020 to investigate the spatial variability of average patterns and extreme events, trends, and their relationship with urban growth and socioeconomic development. The analysis reveals significant spatial variations in precipitation and runoff rates, with higher altitude areas, such as the Serra da Mantiqueira, exhibiting higher average values. Moreover, the Mann–Kendall trend results showed in most of the sites no significant trend regarding precipitation data; however, about 50% of the sites in the PSRB presented a decreasing trend of runoff rates. Since the precipitation does not explain identified changes in the hydrological patterns, the evaluation of the area's urban growth and socioeconomic development throughout the decades suggested that human activities, such as those associated with urbanization, have played a significant role in altering the runoff patterns in the basin. These findings highlight the importance of sustainable land-use planning and water resource-management practices in the PSRB to mitigate the negative impacts of urbanization on the hydrological cycle and to enhance the resilience of the region's water resources. [ABSTRACT FROM AUTHOR]

Purpose: The objective was to analyze the capillary glucose (CG) responses of two resistance exercise intensities in type 2 diabetes mellitus (T2DM) individuals undergoing different drug therapies. Methods: Twelve non-insulin treated (NIT), and six insulin-treated (IT) T2DM individuals (67 ± 7 years) performed two resistance-training sessions (RT-session) at moderate-intensity (MOD, 3 × 10 reps at 70% of 10-RM), high-intensity (HIGH, 3 × 10 reps at 100% of 10-RM), and a non-activity control situation (CONT). The CG was evaluated before, during, and immediately after the experimental situations. Results: Both MOD and HIGH promoted a superior reduction of CG than CONT in NIT (−37 vs. −33 vs. −4 mg/dl, respectively, p < 0.01). Conversely, in RT-sessions, CG reduction was not statistically different when compared to CONT in IT (−51 vs. −45 vs. −20 mg/dl, respectively, p > 0.05). The higher glycemic reduction was found immediately after rather than during the RT session (p < 0.05). In both RT-sessions, pre-exercise CG levels were directly related to CG reduction (p < 0.01). Conclusions: A single acute RT-session reduces CG, regardless of intensity, in NIT individuals. In IT individuals, the RT-session did not induce an additional effect on CG. Moreover, participants with the highest pre-exercise CG levels were the most benefited ones by RT-session in reducing glycemia. [ABSTRACT FROM AUTHOR]

The last two decades have witnessed a strong momentum toward integration of cell‐based and computational approaches in safety assessments. This is fueling a global regulatory paradigm shift toward reduction and replacement of the use of animals in toxicity tests while promoting the use of new approach methodologies. The understanding of conservation of molecular targets and pathways provides an opportunity to extrapolate effects across species and ultimately to determine the taxonomic applicability domain of assays and biological effects. Despite the wealth of genome‐linked data available, there is a compelling need for improved accessibility, while ensuring that it reflects the underpinning biology. We present the novel pipeline Genes‐to‐Pathways Species Conservation Analysis (G2P‐SCAN) to further support understanding on cross‐species extrapolation of biological processes. This R package extracts, synthetizes, and structures the data available from different databases, that is, gene orthologs, protein families, entities, and reactions, linked to human genes and respective pathways across six relevant model species. The use of G2P‐SCAN enables the overall analysis of orthology and functional families to substantiate the identification of conservation and susceptibility at the pathway level. In the present study we discuss five case studies, demonstrating the validity of the developed pipeline and its potential use as species extrapolation support. We foresee this pipeline will provide valuable biological insights and create space for the use of mechanistically based data to inform potential species susceptibility for research and safety decision purposes. Environ Toxicol Chem 2023;42:1152–1166. © 2023 UNILEVER GLOBAL IP LTD. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. [ABSTRACT FROM AUTHOR]

Impairments in several domains of cognitive functions are observed in people with Type 2 Diabetes Mellitus (T2DM), often accompanied by low Brain-derived neurotrophic factor (BDNF) concentrations. Although aerobic and resistance exercise enhances cognitive functions and raises BDNF concentrations in several populations, it remained uncertain in T2DM subjects. This study compared the effects of a single bout of aerobic (AER, 40 min of treadmill walk at 90–95% of the maximum walk speed) or resistance (RES, 3 × 10 repetitions in eight exercises at 70% of 10-RM) exercise on specific cognitive domain performance and plasma BDNF concentrations of physically active T2DM subjects. Eleven T2DM subjects (9 women/2 men; 63 ± 7 years) performed two counterbalanced trials on non-consecutive days. Stroop Color and Word (SCW) task [assessing the attention (congruent condition) and inhibitory control (incongruent condition)], Visual response time (assessing the response time), and blood collection (for plasma BDNF concentrations) were performed pre and post-exercise sessions. With distinct magnitude, both AER and RES improved the incongruent-SCW (d = − 0.26 vs. − 0.43 in AER and RES, respectively; p < 0.05), RT(best) (d = − 0.31 vs. − 0.52, p < 0.05), and RT(1–5) (d = − 0.64 vs. − 0.21, p < 0.05). The congruent-SCW and RT(6–10) were not statistically different. Plasma BDNF concentrations were elevated 11% in AER (d = 0.30) but decreased by 15% in RES (d = − 0.43). A single session of aerobic or resistance exercise similarly improved the inhibitory control and response time of physically active T2DM subjects. Nevertheless, aerobic and resistance exercise sessions induced an opposite clinical effect in plasma BDNF concentrations. [ABSTRACT FROM AUTHOR]

The encapsulation of antifouling compounds, such as DCOIT (4,5-Dichloro-2-octylisothiazol-3(2H)-one), in mesoporous silica nanocapsules (SiNC) has recently been demonstrated to be an eco-friendly alternative to decrease biocide toxicity towards marine non-target species. However, the lack of information on the chronic effects of such nanomaterials on non-target tropical species is critical for a more comprehensive environmental risk assessment. Thus, the present study aimed to assess the chronic toxicity and hazard of the soluble and encapsulated forms of DCOIT on neotropical marine species. Chronic tests were conducted with six ecologically relevant species. No effect concentration (NOEC) values were combined with NOEC values reported for tropical species to assess the hazard using the probabilistic approach to derive each predicted no effect concentration (PNEC). The SiNC-DCOIT was three- to ten-fold less toxic than soluble DCOIT. Probabilistic-based PNECs were set at 0.0001 and 0.0097 µg DCOIT L−1 for the biocide soluble and nanostructured forms, respectively. The immobilization of DCOIT into SiNC led to an 84-fold hazard decrease, confirming that the encapsulation of DCOIT into SiNC is a promising eco-friendly alternative technique, even in a chronic exposure scenario. Therefore, the present study will contribute to better management of the environmental risk of such innovative products in the tropical marine environment. [ABSTRACT FROM AUTHOR]

The Romanesque church of Nossa Senhora de Guadalupe of Mouçós e Lamares is located in Vila Real (North of Portugal). The exterior part of its nave is preserved almost unaltered. Most of the granite ashlars and corbels that make up this church have a mason’s mark in the centre of their faces. The building granites (Sanguinhedo and Vale das Gatas) have been identified and characterised petrographically and petrophysically. The mason’s marks have been as well identified; all the ashlars with visible mason’s marks have been mapped, and a glyptographic study has been carried out. In addition, the surface roughness of ashlars was measured. All these analyses have made it possible to locate the main historical quarry, to calculate the number of stonemasons who worked in the construction of the church, and to determine its construction phases. There are eight main types of mason’s marks on the nave façades. The quarrymen extracted the main building granite (Sanguinhedo granite) from the same quarry, or from nearby quarries. Although the most experienced stonemason has been identified, most stonemasons worked as a team during all construction phases of the church. Techniques such as petrography, ultrasonic P wave velocity, colourimetry, roughness and the determination of hydric properties will guarantee the quality and durability of the heritage stone for restorations. In addition, the glyptographic analysis revealed important historical and ethnographic findings that will be very useful for the appreciation of the monument. Therefore, the knowledge of built heritage, such as mason’s marks contribute to the conservation of historical quarries and traditions, as well as help understand the close cultural association societies have had with heritage stones. In this way, the scientific corpus of historical-cultural heritage can face for the development of tourism in Galicia-North Portugal Euroregion in a long-lasting sustainable way. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the impact of mental fatigue on heart rate variability, subjective measures of fatigue, and swimming performance in young athletes. Methods: Sixteen swimmers [15.45 (0.51) y old, 7.35 (2.20) y of swimming experience] performed a 1500-m time trial on 2 occasions separated by an interval of at least 72 hours. The 1500-m swimming was preceded by a 30-minute treatment that consisted of performing the Stroop Color and Word test to induce mental fatigue (experimental trial) or watching an emotionally neutral video (control trial). Results: Participants reported higher ratings of mental fatigue and mental effort following the Stroop test when compared with the control trial, but no differences in motivation were observed. The induction of mental fatigue impaired swimming performance, as evidenced by a slower time (1.2%) to complete the 1500-m trial. No intertrial differences were identified for rating of perceived exertion during the swimming test or in heart rate variability after the Stroop and swimming tests. Conclusion: The results indicate that induction of mental fatigue impairs 1500-m swimming performance without changing heart rate variability. [ABSTRACT FROM AUTHOR]

Heterogeneous catalysis and anaerobic syngas fermentation represent two different approaches for the conversion of synthesis gas into chemicals and fuels. This review provides a unique comparison of different reaction paths for the fixation of CO2, CO and H2 into elementary building blocks such as methanol, acetic acid and ethanol. Operating conditions, reactor engineering, influence of gas impurities, yields, conversion efficiencies as well as downstream product recovery are compared. It was found that mass‐specific productivity ranges in the same order of magnitude for both technologies, while space‐time yield of heterogeneous catalysis is up to three orders of magnitude higher. [ABSTRACT FROM AUTHOR]

This review presents a comprehensive compilation of toxicological and environmental data, further used to assess the ecological risk (ERA) of the 11 EU approved antifouling biocides (PT21). Diuron, chlorothalonil, DCOIT, and pyrithiones are amongst the most toxic biocides toward producer species, while, CuSCN, medetomidine, and zineb showed higher toxicity toward consumers. In addition, medetomidine, CuSNC, zineb, Cu2O, and chlorothalonil are up to 400-fold more toxic to non-target organisms than target organisms highlighting their potential threat for marine ecosystems. Biocides hazard, which was assessed based on the marine PNECprobabilistic for biocides having enough and reliable ecotoxicological data, can be summarized in the following order: ZnPT (more hazardous) > CuPT > DCOIT > diuron > chlorothalonil > dichlofluanid > tolylfluanid. For the remaining biocides, the marine hazard was calculated based on the PNECdeterministic as follows: CuSCN (more hazardous) > Cu2O > medetomidine > zineb. The risk assessment indicates that the following biocides poses risk (quotient >1) for the coastal ecosystems: DCOIT, diuron, dichlofluanid, chlorothalonil, CuSCN, Cu2O, medetomidine and zineb. In order to protect the services and functions of coastal environments, a systematic and continuous monitoring of these biocides in coastal areas are highly recommended. Current regulatory framework and the replacement of state-of-the-art biocides by safer alternative is also critically discussed. [ABSTRACT FROM AUTHOR]

In order to increase the typically low equilibrium CO2 conversion to methanol using commercially proven technology, the addition of two intermediate condensation units between reaction steps is evaluated in this work. Detailed process simulations with heat integration and techno-economic analyses of methanol synthesis from green H2 and captured CO2 are presented here, comparing the proposed process with condensation steps with the conventional approach. In the new process, a CO2 single-pass conversion of 53.9% was achieved, which is significantly higher than the conversion of the conventional process (28.5%) and its equilibrium conversion (30.4%). Consequently, the total recycle stream flow was halved, which reduced reactant losses in the purge stream and the compression work of the recycle streams, lowering operating costs by 4.8% (61.2 M€·a−1). In spite of the additional number of heat exchangers and flash drums related to the intermediate condensation units, the fixed investment costs of the improved process decreased by 22.7% (94.5 M€). This was a consequence of the increased reaction rates and lower recycle flows, reducing the required size of the main equipment. Therefore, intermediate condensation steps are beneficial for methanol synthesis from H2/CO2, significantly boosting CO2 single-pass conversion, which consequently reduces both the investment and operating costs. [ABSTRACT FROM AUTHOR]

The cricetid rodent genus Rhipidomys belongs to the tribe Thomasomyini, subfamily Sigmodontinae, whose distribution covers high-and lowlands areas of South America. In a number of contributions to the taxonomy and systematics of the genus, 24 species of Rhipidomys were recognized by different authors until now. Here we identify 13 species of the lowland "leucodactylus" section from Brazil and named two new forms of this genus based on morphological and molecular data. One of these forms is only found at municipality of Bezerros, Pernambuco state, while the other has a more extensive distribution, being recorded in northern Goiás, west of Tocantins, southern Piauí, western portion of Bahia and northern region of Minas Gerais states. These two new species can be distinguished from the others of the genus by qualitative and quantitative characters although, like most other Brazilian species of Rhipidomys, they do not exhibit an autapomorphic characters. These species are diagnosed by unique combinations of character states that are operational and useful for species recognition. In general, the most valuable character sets to differentiate Rhipidomys species are found in the skull, external morphology being strongly variable and showing overlapping sets of characters. We compared the two new species with all Brazilian species of the "leucodactylus" section and added taxonomic comments and their phylogenetic relationships. [ABSTRACT FROM AUTHOR]

Due to the high acid, oxygen and water contents of fast pyrolysis oil, it requires the improvement of its fuel properties by further upgrading, such as catalytic hydrodeoxygenation (HDO). In this study, Nb2O5 was evaluated as a support of Pd-based catalysts for HDO of fast pyrolysis oil. A Pd/SiO2 catalyst was used as a reference. Additionally, the impact of iron as a promoter in two different loadings was investigated. The activity of the synthesized catalysts was evaluated in terms of H2 uptake and composition of the upgraded products (gas phase, upgraded oil and aqueous phase) through elemental analysis, Karl Fischer titration, GC-MS/FID and 1H-NMR. In comparison to SiO2, due to its acid sites, Nb2O5 enhanced the catalyst activity towards hydrogenolysis and hydrogenation, confirmed by the increased water formation during HDO and a higher content of hydrogen and aliphatic protons in the upgraded oil. Consequently, the upgraded oil with Nb2O5 had a lower average molecular weight and was therefore less viscous than the oil obtained with SiO2. When applied as a promoter, Fe enhanced hydrogenation and hydrogenolysis, although it slightly decreased the acidity of the support, owing to its oxophilic nature, leading to the highest deoxygenation degree (42.5 wt.%) and the highest product HHV (28.2 MJ/kg). [ABSTRACT FROM AUTHOR]

Establishing a NexGen, mechanism-based environmental risk assessment paradigm shift: Are we ready yet? Traditional (eco)toxicology has reached a tipping point, with the historical reliance on animal tests for assessing safety being rapidly superseded by the quest for alternative approaches that take advantage of the latest science. Toward an integrated strategy to understand mechanisms across species for chemical safety assessment. [Extracted from the article]

Recent advances in nanotechnology have allowed the encapsulation of hazardous antifouling (AF) biocides in silica mesoporous nanocapsules (SiNC) reducing their short-term toxicity. However, the chronic effects of such novel nanoadditives remain understudied. The present study aimed to assess short- and long-term sub-lethal effects of soluble forms (DCOIT and Ag) and nanostructured forms (SiNC-DCOIT and SiNC-DCOIT-Ag) of two AF biocides and the "empty" nanocapsule (SiNC) on juveniles of Crassostrea gigas after 96 h and 14 days of exposure. Juvenile oysters exposed for a short period to free DCOIT and AgNO3 presented worse physiological status comparing with those exposed to the nanostructured forms. The long-term exposure to DCOIT and Ag+ caused an extensive biochemical impairment comparing with the tested nanomaterials, which included oxidative damage, activation of the antioxidant defense system, and neurotransmission impairment. Despite the negative effects mostly observed on the health condition index and AChE, the encapsulation of the abovementioned AF biocides into SiNC seems to be a technological advantage towards the development of AF nanoadditives with lower long-term toxicity comparing with the soluble forms of such biocides. [ABSTRACT FROM AUTHOR]

Staphylococcus aureus is a leading cause of bovine mastitis worldwide. Despite some improved understanding of disease pathogenesis, progress towards new methods for the control of intramammary infections (IMI) has been limited, particularly in the field of vaccination. Although herd management programs have helped to reduce the number of clinical cases, S. aureus mastitis remains a major disease burden. This review summarizes the past 16 years of research on bovine S. aureus population genetics, and molecular pathogenesis that have been conducted worldwide. We describe the diversity of S. aureus associated with bovine mastitis and the geographical distribution of S. aureus clones in different continents. We also describe studies investigating the evolution of bovine S. aureus and the importance of host-adaptation in its emergence as a mastitis pathogen. The available information on the prevalence of virulence determinants and their functional relevance during the pathogenesis of bovine mastitis are also discussed. Although traits such as biofilm formation and innate immune evasion are critical for the persistence of bacteria, the current understanding of the key host-pathogen interactions that determine the outcome of S. aureus IMI is very limited. We suggest that greater investment in research into the genetic and molecular basis of bovine S. aureus pathogenesis is essential for the identification of novel therapeutic and vaccine targets. [ABSTRACT FROM AUTHOR]

Campos, BT, Penna, EM, Rodrigues, JGS, Mendes, TT, Maia-Lima, A, Nakamura, FY, Vieira, ÉLM, Wanner, SP, and Prado, LS. Influence of mental fatigue on physical performance, and physiological and perceptual responses of judokas submitted to the Special Judo Fitness Test. J Strength Cond Res 36(2): 461–468, 2022—Mentally fatigued athletes present impaired aerobic performance, strength endurance, and manual dexterity, despite no changes in anaerobic performance and maximal muscle strength and power. Noteworthy, the effect of mental fatigue on physical performance during high-intensity intermittent tests that require specific motor skills of fighting sports has not been investigated. Therefore, this study aimed to verify whether mental fatigue influences performance and physiological and perceptual responses of judokas subjected to a high-intensity intermittent test designed specifically and validated for judo. Each judoka performed 2 experimental trials—a control trial one and the other one after the induction of mental fatigue. These trials were scheduled in a random and balanced order. In both trials, lactate, glucose, and cortisol concentrations, the heart rate variability, and perceptual variables were collected after the initial treatment and after the Special Judo Fitness Test (SJFT). The initial treatment consisted of a 30-minute cognitive demanding task (Stroop Color test) or watching a movie (control) and was followed by the SJFT. The Stroop Color test increased the perceptions of mental fatigue and effort, without affecting motivation for subsequent testing. Unexpectedly, mentally fatigued athletes did not show reduced performance during the SJFT. Regarding the physiological variables, no significant differences were identified between the 2 experimental conditions. We conclude that physical performance measured during a specific test for judokas is not impaired by a previous 30-minute cognitive task that causes mental fatigue. In addition, this cognitive task did not influence the physiological changes induced by the specific physical test. [ABSTRACT FROM AUTHOR]

The cleaning or removal of manganese stains on Cultural Heritage has not been much tested or successful so far. The aim of this article was to assess a new green cleaning gel for Mn-rich black-blue stains on different substrates. The black-blue stains were characterized at optical and chemical level through colour-related data, optical microscope, FTIR, XRF and XPS. Mn-stained granite found on historical churches at Vila Real (North of Portugal) and glass jars of Leclanché cells, belonging to the ISEP's Museum (Portugal) collection, were the ideal case studies to test the efficiency of chitosan: thiourea dioxide (TD) cleaning gel. TD proved to be the best candidate to reduce insoluble manganese oxides, over Hydroxylamine Hydrochloride and Hydroxymethanesulfinic Acid. Cleaning assays performed on stained granite samples collected at a historical quarry and in situ application on stained granite churches allowed removal of the stains to a satisfactory level. Similar results were obtained on stained glass jars. [ABSTRACT FROM AUTHOR]

Background: The Wingate test is usually conducted using resistance loads of 7.5, 8.5 or 8.7 g.kg−1 of body mass. However, these cycle ergometer resistance loads may be insufficient to enable maximal power values. As such, the use of individual optimal load selection (OPT) can positively influence other Wingate test parameters. Moreover, anthropometric parameters, e.g. body mass index, may also affect the OPT, but have not been well investigated. Aim: To investigate (i) individual OPT on the Wingate Test for peak power (PP), mean power outputs (MP), and the fatigue index (FI) and (ii) the association between OPT and anthropometric parameters. Methods: Fifteen physically active men (age = 26.4 ± 4.1 yr) performed six 30-s Wingate tests, with loads corresponding to 6.5%, 8.5%, 10.5% 11.5% 12.5%, and 13.5% of body mass to determine OPT (the load corresponding with the highest peak power), and also PP, MP, and FI. Results: Peak Power was 15% higher (p<0.001) and FI was 21% higher (p=0.03) for the OPT method, whereas Mean Power values were similar between OPT and the 8.5% of body mass load. The 8.5% of body mass resistance load underestimates power outputs and is directly related to individual Peak Power (r=0.58, p=0.02). Furthermore, the load corresponding to OPT was inversely correlated to body mass index (r=−0.81, p<0.001) and percent body fat (r=−0.51, p=0.04). Conclusion: Optimal load selection is more appropriate than the use of 8.5% of body mass load to enable higher Peak power in the 30-s Wingate test in a physically active male population. [ABSTRACT FROM AUTHOR]

Copyright of Conservar Património is the property of Associacao Profissional de Conservadores-Restauradores de Portugal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Transcriptomic responses combined with apical adverse ecologically relevant outcomes have proven to be useful to unravel and anchor molecular mechanisms of action to adverse outcomes. This is the case for feeding inhibition responses in the model ecotoxicological species Daphnia magna. The aim of the present study was to assess the transcriptomic responses in guts dissected from D. magna individuals exposed to concentrations of selected compounds that inhibit feeding and compare them with the responses associated to 2 levels of food restriction (low food and starvation). Chemical treatments included cadmium, copper, fluoranthene, λ‐cyhalothrin, and the cyanotoxin anatoxin‐a. Although the initial hypothesis was that exposure to chemical feeding inhibitors should elicit similar molecular responses as food limitation, the corresponding gut transcriptomic responses differed significantly. In moderate food limitation conditions, D. magna individuals increased protein and carbohydrate catabolism, likely to be used as energetic sources, whereas under severe starving conditions most metabolism‐related pathways appeared down‐regulated. Treatment with chemical feeding inhibitors promoted cell turnover–related signaling pathways in the gut, probably to renew tissue damage caused by the reported oxidative stress effects of these compounds, and inhibited the transcription of gut digestive gene enzymes and energetic metabolic pathways. We conclude that chemical feeding inhibitors, rather than mimicking the physiological response to low‐ or no‐food conditions, cause specific toxic effects, preventing Daphnia both from feeding and from adjusting its metabolism to the resulting low energy intake. Environ Toxicol Chem 2021;40:2510–2520. © 2021 SETAC [ABSTRACT FROM AUTHOR]

Background: Mental fatigue is a psychobiological state caused by extended periods of cognitive effort, and evidence suggests that mentally fatigued athletes present impaired physical performance. Different ergogenic aids have been proposed to counteract the deleterious effects of mental fatigue, but whether brain stimulation can counteract mental fatigue is still unknown. This scenario is even more obscure considering the effects of these interventions (mental fatigue induction and brain stimulation) in a very experienced population consisting of master athletes. Method: Ten master swimmers (30 ± 6 years old and 14 ± 8 years of experience) participated in the study. They underwent four experimental conditions before an 800-m freestyle test: mental fatigue with brain stimulation; mental fatigue without brain stimulation; absence of mental fatigue with brain stimulation; and absence of mental fatigue and no brain stimulation. Mental fatigue was induced by a cognitively demanding Stroop Color Test, whereas stimulation was applied on the temporal cortex. After that, the athletes swan 800 m as fast as possible and provided their ratings of perceived exertion (RPE) every 200 m. Results: Mental fatigue was effectively induced, as evidenced by a greater fatigue perception and more errors in the last blocks of the cognitive task. Mental fatigue induction did not influence performance (time to complete the swimming trial) and RPE. Similarly, brain stimulation failed to change these two parameters, regardless of mental fatigue induction. Conclusion: The prolonged physical performance of experienced master athletes is not influenced, under the present conditions, by mental fatigue induction, cerebral stimulation, and their association. [ABSTRACT FROM AUTHOR]

This investigation evaluated the bioaccumulation potential of the tropical estuarine bivalve Anomalocardia flexuosa for trace metals. To this aim, chemical and sedimentological analyses and bioaccumulation tests were performed. The sediments were mainly composed by fine-sands and mud, with variable levels of organic matter and CaCO3. Muddy sediments from a depositional site (P2) presented the highest concentrations of metals, despite SEM/AVS not indicating bioavailability. Bioaccumulation factors showed high ratios for Cd, Ni, and Zn, while associations between the contents of mud, organic matter, CaCO3 and metals in sediments and tissues of A. flexuosa were indicated by a principal component analysis. The SEM/AVS was not effective to predict the bioavailability through dissolved metals. The results showed that contaminants were bioavailable, while the performed bioaccumulation test proved to be a reliable technique for assessing sediment contamination in estuarine regions. Moreover, A. flexuosa was considered an adequate test organism for bioaccumulation studies. [ABSTRACT FROM AUTHOR]

Copyright of Enfermagem Brasil is the property of Atlantica Editora and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Featured Application: The present study can foster nanoecotoxicology research on tropical environments and can be also applied on the maritime industry. The encapsulation of the biocide DCOIT in mesoporous silica nanocapsules (SiNC) has been applied to reduce the leaching rate and the associated environmental impacts of coatings containing this biocide. This research aimed to evaluate the effects of DCOIT in both free and nanostructured forms (DCOIT vs. SiNC-DCOIT, respectively) and the unloaded SiNC on different life stages of the bivalve Perna perna: (a) gametes (fertilization success), (b) embryos (larval development), and (c) juveniles mussels (byssus threads production and air survival after 72 h of aqueous exposure). The effects on fertilization success showed high toxicity of DCOIT (40 min-EC50 = 0.063 μg L−1), followed by SiNC-DCOIT (8.6 μg L−1) and SiNC (161 μg L−1). The estimated 48 h-EC50 of SiNC, DCOIT and SiNC-DCOIT on larval development were 39.8, 12.4 and 6.8 μg L−1, respectively. The estimated 72 h-EC50 for byssus thread production were 96.1 and 305.5 µg L−1, for free DCOIT and SiNC-DCOIT, respectively. Air survival was significantly reduced only for mussels exposed to free DCOIT. Compared to its free form, SiNC-DCOIT presented a balanced alternative between efficacy and toxicity, inhibiting efficiently the development of the target stage (larvae that is prone to settle) and satisfactorily preventing the juvenile attachment. [ABSTRACT FROM AUTHOR]

The fifth basic taste—umami—is based on hydrophilic monosodium L-glutamate (L-Glu), and, to a smaller extent, on L-aspartate (L-Asp), which can be found in free form in seaweeds. The umami taste can be elicited by the synergistic effect of 5′-nucleotides, guanosine-5′-monophosphate (GMP), and inosine-5′-monophosphate (IMP). In this work, twelve edible seaweeds, two green (Codium tomentosum, Ulva rigida), six red (Chondracanthus teedei var. lusitanicus, Chondrus crispus, Gracilaria gracilis, Grateloupia turuturu, Nemalion helminthoides, Osmundea pinnatifida), and four brown (Bifurcaria bifurcata, Fucus vesiculosus, Saccorhiza polyschides, Undaria pinnatifida), from the Portuguese seashore, were selected for the analysis of umami amino acids and 5′-nucleotides. To determine the concentrations of umami free compounds, L-Glu, L-Asp, GMP, and IMP, the usual procedure for the preparation of seaweed's broth was carried out, and the resulting extracts were analyzed by HPLC-DAD. The effect of the seaweed's pretreatment, oven-dried and lyophilized, was also studied. Results showed that all seaweed species contained free amino acids in broad range, whereas umami 5′-nucleotides were not detected. The lyophilized C.crispus and G. gracilis contained the highest amounts of free L-Glu (627 ± 109 mg (100 g)−1 and 398 ± 67 mg (100 g)−1, respectively) and L-Asp (520 ± 102 mg (100 g)−1 and 270 ± 10 mg (100 g)−1, respectively). Two-factor PCA explained quantitatively (90%) the variance of seaweed species based on umami L-Glu content after different drying techniques applied. The results indicate that lyophilization constitutes a superior procedure for increasing the umami potential in most of the selected seaweeds. [ABSTRACT FROM AUTHOR]

Copyright of Geo UERJ is the property of Editora da Universidade do Estado do Rio de Janeiro (EdUERJ) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To evaluate the interactions of metabolic neuronal‐glial changes with the presence and hemispheric‐side of hippocampal sclerosis (HS) and its potential role in predicting pharmacoresistance in temporal lobe epilepsy (TLE). Methods: We included structural magnetic resonance imaging (MRI) and proton magnetic resonance spectroscopy (1H‐MRS) metabolic data for 91 patients with unilateral TLE and 50 healthy controls. We measured the values of total N‐acetyl aspartate/total creatine (tNAA/tCr), glutamate/tCr (Glu/tCr), and myo‐inositol/tCr (mIns/tCr). To assess the influence of the pharmacoresponse and hemispheric‐side of HS on metabolic data, the relationship between clinical and MRI data, and the predictive value of NAA/Cr, we used analysis of variance/covariance and built a logistic regression model. We used bootstrap simulations to evaluate reproducibility. Results: Bilateral tNAA/tCr reduction was associated with pharmacoresistance and with left HS, a decrease of Glu/tCr ipsilateral to the seizure focus was associated with pharmacoresistance, and ipsilateral mIns/tCr increase was related to pharmacoresistance and the presence of left HS. The logistic regression model containing clinical and 1H‐MRS data discriminated pharmacoresistance (area under the curve [AUC] = 0.78). However, the reduction of tNAA/tCr was the main predictor, with the odds 2.48 greater for pharmacoresistance. Significance: Our study revealed a spectrum of neuronal‐glial changes in TLE, which was associated with pharmacoresistance, being more severe in left‐sided HS and less severe in MRI‐negative TLE. These noninvasive, in vivo biomarkers provide valuable additional information about the interhemispheric differences in metabolic dysfunction, seizure burden, and HS, and may help to predict pharmacoresistance. [ABSTRACT FROM AUTHOR]

This short note aims to report in detail a preliminary assessment of the concentrations of Cd, Hg and Pb in tissues of blue crabs Callinectes danae collected from the Cananéia-Iguape-Peruíbe estuarine complex (CIP), in the South of São Paulo State coast, Brazil. In October 2014, blue crabs were collected from CIP. Tissues were removed by dissection and metal determination was performed by GF-AAS and CV-AAS. According to statistical analysis, Pb and Cd concentrations in gills were significantly higher than those found in muscles and hepatopancreas, respectively. There were no significant differences in Hg concentrations between samples. Cd, Hg and Pb concentrations in gills and hepatopancreas were lower than those reported in a previous study performed at CIP. However, Cd concentration in hepatopancreas was higher than the Brazilian limit for consumption and new efforts to monitor Cd concentrations in C. danae tissues must be performed. [ABSTRACT FROM AUTHOR]

Judo is a high-intensity intermittent combat sport which causes cardiac adaptations both morphologically and related to the autonomic nervous system (ANS). Therefore, this study aims to verify the correlation between heart rate variability (HRV) at rest with performance in the Special Judo Fitness Test (SJFT) and whether groups with different RR values at rest show different performance in the SJFT and during post-test recovery. Sixteen judo athletes with 7.2 ± 3.9 years of training experience participated in the study. Before and after the SJFT execution HRV and lactate measurements were conducted. For HRV analysis, we used the mean interval RR, the standard deviation of the RR interval (SDNN), the root mean square of successive differences in RR intervals (RMSSD), the low frequency (LF) and high frequency (HF) in normalized and absolute units. The sample was split into two groups (low RR and high RR) to verify if this variable could differentiate between specific performance. For the SDNN, a significant and moderate correlation (r = 0.53) was found with the total number of throws and throws in the series A (r = 0.56) and B (r = 0.54) and for the RMSSD a correlation with throws during series B (r = 0.59) in the SJFT. However, the groups did not differ in performance and recovery. Therefore, HRV is related to intermittent judo performance; however, it cannot differentiate between judokas at different levels of performance. [ABSTRACT FROM AUTHOR]

Copyright of Revista CIATEC-UPF is the property of Revista CIATEC-UPF and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract: The performances of gas chromatography with mass spectrometry and of comprehensive two‐dimensional gas chromatography with time‐of‐flight mass spectrometry are examined through the comparison of Daphnia magna metabolic profiles. Gas chromatography with mass spectrometry and comprehensive two‐dimensional gas chromatography with mass spectrometry were used to compare the concentration changes of metabolites under saline conditions. In this regard, a chemometric strategy based on wavelet compression and multivariate curve resolution–alternating least squares is used to compare the performances of gas chromatography with mass spectrometry and comprehensive two‐dimensional gas chromatography with time‐of‐flight mass spectrometry for the untargeted metabolic profiling of Daphnia magna in control and salinity‐exposed samples. Examination of the results confirmed the outperformance of comprehensive two‐dimensional gas chromatography with time‐of‐flight mass spectrometry over gas chromatography with mass spectrometry for the detection of metabolites in D. magna samples. The peak areas of multivariate curve resolution–alternating least squares resolved elution profiles in every sample analyzed by comprehensive two‐dimensional gas chromatography with time‐of‐flight mass spectrometry were arranged in a new data matrix that was then modeled by partial least squares discriminant analysis. The control and salt‐exposed daphnids samples were discriminated and the most relevant metabolites were estimated using variable importance in projection and selectivity ratio values. Salinity de‐regulated 18 metabolites from metabolic pathways involved in protein translation, transmembrane cell transport, carbon metabolism, secondary metabolism, glycolysis, and osmoregulation. [ABSTRACT FROM AUTHOR]

Background: Unravelling the link between genes and environment across the life cycle is a challenging goal that requires model organisms with well-characterized life-cycles, ecological interactions in nature, tractability in the laboratory, and available genomic tools. Very few well-studied invertebrate model species meet these requirements, being the waterflea Daphnia magna one of them. Here we report a full genome transcription profiling of D. magna during its life-cycle. The study was performed using a new microarray platform designed from the complete set of gene models representing the whole transcribed genome of D. magna. Results: Up to 93% of the existing 41,317 D. magna gene models showed differential transcription patterns across the developmental stages of D. magna, 59% of which were functionally annotated. Embryos showed the highest number of unique transcribed genes, mainly related to DNA, RNA, and ribosome biogenesis, likely related to cellular proliferation and morphogenesis of the several body organs. Adult females showed an enrichment of transcripts for genes involved in reproductive processes. These female-specific transcripts were essentially absent in males, whose transcriptome was enriched in specific genes of male sexual differentiation genes, like doublesex. Conclusion: Our results define major characteristics of transcriptional programs involved in the life-cycle, differentiate males and females, and show that large scale gene-transcription data collected in whole animals can be used to identify genes involved in specific biological and biochemical processes. [ABSTRACT FROM AUTHOR]

The artcle provides information on role of omics technologies in enhancing regulations of chemical safety and molecular landscape. Topics discussed include adverse outcome pathways (AOPs) and academic perspectives; Organisation for Economic Co-operation and Development (OECD); and biological replication.

Copyright of Anuario do Instituto de Geociencias is the property of Universidade Federal do Rio de Janeiro, Instituto de Geociencias and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Revista Metropolitana de Sustentabilidade is the property of Revista Metropolitana de Sustentabilidade - RMS and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We performed a set of numerical experiments for a severe extratropical storm that hit the United Kingdom in December 2013 using the Weather Research and Forecasting (WRF) model in a nested setup. Four experiments were performed, using the original orography (ORG), a 50% increase in orography (T50), a 100% increase (i.e. double; T100), and a total removal (flattening) of the mountainous terrain (RMV) over Wales. The results show that rainfall amounts and distribution are sensitive to the modifications made. Moreover, the analysis of the vertical distribution of water vapor, as well as vertical velocities, indicates that higher mountains act as a windbreak and confine moisture to the windward side. Lower mountains let moisture flow and contribute to rain patterns in England (leeward side). Changes in Mean Sea Level Pressure (MSLP) were also observed, and the results agree with rainfall amounts. Furthermore, the time series of the spatial average of Convective Available Potential Energy (CAPE) suggests that orography has a stronger influence on storm dynamics than it does on storm genesis. Increased orography results in deeper storms and intensifies vertical flows, changing the dynamics and the energy of the environment. [ABSTRACT FROM AUTHOR]

Copyright of Pegada is the property of Pegada and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In this work carbon quantum dot (CQD) nanoparticles are synthesized from d-lactose using a hydrothermal method and then they are coated with polyethylene glycol (CQDs@PEG). These particles exhibit a monodisperse spherical morphology with an average particle size of ∼4 nm. Nuclear magnetic resonance (NMR) and Fourier transform infrared (FTIR) spectroscopy showed the presence of the hydroxyl groups from the ethylene glycol molecules grafted onto the CQDs’ surfaces, which confirms that PEG was covalently attached to the nanoparticles’ surfaces. Fluorescence analysis demonstrates a shift in the emission at 495 nm after PEG coating. Modified carbon dots were introduced into thermo-responsive pNIPAM microgels. The resultant pNIPAM–CQDs@PEG hybrid system exhibits interesting fluorescence properties. Transmission electron microscopy (TEM), fluorescence microscopy, and energy-dispersive X-ray (EDX) spectroscopy confirm the incorporation of CQD particles into the microgels. Finally, dynamic light scattering (DLS) analysis confirms that further hybrid microgels based on pNIPAM are thermo-responsive, with a transition temperature similar to that of a system with an ionic component. [ABSTRACT FROM AUTHOR]

Lactococcus lactis subsp. lactis NCDO 2118 was recently reported to alleviate colitis symptoms via its anti-inflammatory and immunomodulatory activities, which are exerted by exported proteins that are not produced by L. lactis subsp. lactis IL1403. Here, we used in vitro and in silico approaches to characterize the genomic structure, the safety aspects, and the immunomodulatory activity of this strain. Through comparative genomics, we identified genomic islands, phage regions, bile salt and acid stress resistance genes, bacteriocins, adhesion-related and antibiotic resistance genes, and genes encoding proteins that are putatively secreted, expressed in vitro and absent from IL1403. The high degree of similarity between all Lactococcus suggests that the Symbiotic Islands commonly shared by both NCDO 2118 and KF147 may be responsible for their close relationship and their adaptation to plants. The predicted bacteriocins may play an important role against the invasion of competing strains. The genes related to the acid and bile salt stresses may play important roles in gastrointestinal tract survival, whereas the adhesion proteins are important for persistence in the gut, culminating in the competitive exclusion of other bacteria. Finally, the five secreted and expressed proteins may be important targets for studies of new anti-inflammatory and immunomodulatory proteins. Altogether, the analyses performed here highlight the potential use of this strain as a target for the future development of probiotic foods. [ABSTRACT FROM AUTHOR]

The authors set up a protocol to perform a 2-generational ring test using the existing guidelines for the Daphnia magna reproduction test. It is well known in ecology that size and quality of offspring vary across the first clutches in D. magna and that certain chemicals affect offspring quality. Therefore, the origin of the second generation is an important factor to consider. Two-generational effects across first, second, and third clutches were evaluated using 4-nonylphenol; those across first and third clutches were evaluated using tributyltin, and those across the third clutch were evaluated using piperonyl butoxide. The compound showing the greatest aggravation of toxic effects between the parental and second generations was piperonyl butoxide, followed by 4-nonylphenol, whereas intergenerational effects of tributyltin varied across experiments. The studied chemicals affected the quantity and quality of the offspring produced by exposed females of the parental generation, those effects being greater in third-clutch neonates. Therefore, when third-clutch offspring were further exposed, they turned out to be more sensitive than the parental generation. The results are in line with those obtained in multigenerational studies using mammalian tests, which showed that, in many cases, effects on the second generation can be predicted by evaluating the quality of the offspring produced. Environ Toxicol Chem 2016;35:1470-1477. © 2015 SETAC [ABSTRACT FROM AUTHOR]