5 results on '"Batista-Gonzalez, Ana"'
Search Results
2. New Insights on the Role of Lipid Metabolism in the Metabolic Reprogramming of Macrophages.
- Author
-
Batista-Gonzalez, Ana, Vidal, Roberto, Criollo, Alfredo, and Carreño, Leandro J.
- Subjects
LIPID metabolism ,FATTY acid oxidation ,MACROPHAGES ,MACROPHAGE activation ,OXIDATIVE phosphorylation - Abstract
Macrophage activation is intimately linked to metabolic reprogramming. Inflammatory (M1) macrophages are able to sustain inflammatory responses and to kill pathogens, mostly by relying on aerobic glycolysis and fatty acid biosynthesis. Glycolysis is a fast way of producing ATP, and fatty acids serve as precursors for the synthesis of inflammatory mediators. On the opposite side, anti-inflammatory (M2) macrophages mediate the resolution of inflammation and tissue repair, switching their metabolism to fatty acid oxidation and oxidative phosphorylation. Over the years, this classical view has been challenged by recent discoveries pointing to a more complex metabolic network during macrophage activation. Lipid metabolism plays a critical role in the activation of both M1 and M2 macrophages. Recent evidence shows that fatty acid oxidation is also essential for inflammasome activation in M1 macrophages, and glycolysis is now known to fuel fatty acid oxidation in M2 macrophages. Ultimately, targeting lipid metabolism in macrophages can improve the outcome of metabolic diseases. Here, we review the main aspects of macrophage immunometabolism from the perspective of the metabolism of lipids. Building a reliable metabolic network during macrophage activation will bring us closer to targeting macrophages for improving human health. [ABSTRACT FROM AUTHOR] more...
- Published
- 2020
- Full Text
- View/download PDF
Catalog
3. Enhanced control of Mycobacterium tuberculosis extrapulmonary dissemination in mice by an arabinomannan-protein conjugate vaccine.
- Author
-
Prados-Rosales, Rafael, Carreño, Leandro, Cheng, Tingting, Blanc, Caroline, Weinrick, Brian, Malek, Adel, Lowary, Todd L., Baena, Andres, Joe, Maju, Bai, Yu, Kalscheuer, Rainer, Batista-Gonzalez, Ana, Saavedra, Noemi A., Sampedro, Leticia, Tomás, Julen, Anguita, Juan, Hung, Shang-Cheng, Tripathi, Ashish, Xu, Jiayong, and Glatman-Freedman, Aharona more...
- Subjects
MYCOBACTERIUM tuberculosis ,BACTERIAL vaccines ,TUBERCULOSIS prevention ,CELLULAR immunity ,LABORATORY mice - Abstract
Currently there are a dozen or so of new vaccine candidates in clinical trials for prevention of tuberculosis (TB) and each formulation attempts to elicit protection by enhancement of cell-mediated immunity (CMI). In contrast, most approved vaccines against other bacterial pathogens are believed to mediate protection by eliciting antibody responses. However, it has been difficult to apply this formula to TB because of the difficulty in reliably eliciting protective antibodies. Here, we developed capsular polysaccharide conjugates by linking mycobacterial capsular arabinomannan (AM) to either Mtb Ag85b or B. anthracis protective antigen (PA). Further, we studied their immunogenicity by ELISA and AM glycan microarrays and protection efficacy in mice. Immunization with either Abg85b-AM or PA-AM conjugates elicited an AM-specific antibody response in mice. AM binding antibodies stimulated transcriptional changes in Mtb. Sera from AM conjugate immunized mice reacted against a broad spectrum of AM structural variants and specifically recognized arabinan fragments. Conjugate vaccine immunized mice infected with Mtb had lower bacterial numbers in lungs and spleen, and lived longer than control mice. These findings provide additional evidence that humoral immunity can contribute to protection against Mtb. [ABSTRACT FROM AUTHOR] more...
- Published
- 2017
- Full Text
- View/download PDF
4. The Type of Growth Medium Affects the Presence of a Mycobacterial Capsule and Is Associated With Differences in Protective Efficacy of BCG Vaccination Against Mycobacterium tuberculosis.
- Author
-
Prados-Rosales, Rafael, Carreño, Leandro J., Weinrick, Brian, Batista-Gonzalez, Ana, Glatman-Freedman, Aarona, Jiayong Xu, Chan, John, Jacobs Jr., William R., Porcelli, Steven A., Casadevall, Arturo, Xu, Jiayong, and Jacobs, William R Jr more...
- Subjects
MYCOBACTERIUM tuberculosis ,MYCOBACTERIAL diseases ,BCG vaccines ,LABORATORY mice ,IMMUNE response ,PATIENTS - Abstract
Background: Bacillus Calmette-Guerin (BCG) vaccine is widely used for the prevention of tuberculosis, despite limited efficacy. Most immunological studies of BCG or Mycobacterium tuberculosis strains grow bacteria in the presence of detergent, which also strips the mycobacterial capsule. The impact of the capsule on vaccine efficacy has not been explored.Methods: We tested the influence of detergent in cultures of BCG and M. tuberculosis strains on the outcome of vaccination experiments on mice and transcriptional responses on M. tuberculosisResults: Vaccination of mice with encapsulated BCG promoted a more potent immune response relative to vaccination with unencapsulated BCG, including higher polysaccharide-specific capsule antibody titers, higher interferon γ and interleukin 17 splenic responses, and more multifunctional CD4(+) T cells. These differences correlated with variability in the bacterial burden in lung and spleen of mice infected with encapsulated or unencapsulated M. tuberculosis The combination of vaccination and challenge with encapsulated strains resulted in the greatest protection efficacy. The transcriptome of encapsulated M. tuberculosis was similar to that of starvation, hypoxia, stationary phase, or nonreplicating persistence.Conclusions: The presence of detergent in growth media and a capsule on BCG were associated with differences in the outcome of vaccination, implying that these are important variables in immunological studies. [ABSTRACT FROM AUTHOR] more...- Published
- 2016
- Full Text
- View/download PDF
5. ANALYSIS OF IN VITRO AND IN VIVO ANTIOXIDANT PROPERTIES OF HYDROPHILIC FRACTIONS FROM THE SEAWEED HALIMEDA MONILE L.
- Author
-
BATISTA-GONZALEZ, ANA ELSA, SILVA, ANA MARA DE OLIVEIRA E, VIDAL-NOVOA, ALEXIS, PINTO, JOSE RICARDO, MANCINI, DALVA ASSUNÇÃO PORTARI, and MANCINI-FILHO, JORGE
- Subjects
ANTIOXIDANTS ,HYDROPHILIC compounds ,MARINE algae ,HALIMEDA ,TERPENES ,FLAVONOIDS ,FIRE assay - Abstract
ABSTRACT In this work, in vitro and in vivo antioxidant properties of the marine algae Halimeda monile were assessed and the levels of some of its compounds likely to be responsible for such properties were determined. The estimated contents of total polyphenols, chlorophylls a and b and carotenoids were 179.5, 356.3, 452.8 and 42.2 µg/g dry weight seaweed, respectively. The presence of terpenoids and flavonoids was also observed. The antioxidant activity of two polar fractions from H. monile (lyophilized aqueous extract and free phenolic acid fraction) was evaluated using three antioxidant assays: ferric reducing antioxidant power, 1,1-diphenyl-2-picrylhydrazyl and lipid peroxidation. Treatment of CCl
4 -induced liver damage in rats with extracts resulted in lower serum thiobarbituric acid-reactive substances levels and higher hepatic glutathione concentrations compared to those observed in the CCl4 -treated group. Also, a significant increase in catalase activity was detected after treatment with the extracts. These results suggest that the seaweed H. monile could be a potential source for natural antioxidants. PRACTICAL APPLICATIONS Seaweeds have a broad chemical composition, consisting of bioactive molecules as chlorophyll, carotenoids, mycosporine-like amino acids, terpenoids, carbohydrates, vitamins and phenolic compounds. Different seaweed extracts have received increased attention, due to their pharmacological effects, particularly in vivo hypolipidemic, antioxidant, immunological and antitumoral activities. The phenolic compounds are powerful antioxidant molecules, and fractions rich in these compounds may be use as antioxidant complement in health states where oxidative state is damage. Results of this research with Halimeda monile L. fractions showed in experiments in vitro and in vivo the presence of different phenolic compounds and that the antioxidant activity is related to them. Therefore, the H. monile L. seaweed fractions have the potential to be developed into new health foods. [ABSTRACT FROM AUTHOR] more...- Published
- 2012
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.