1. Bioefficacy of Bacillus cereus and its Three Mutants by UV Irradiation Against Meloidogyne incognita and Gene Expression in Infected Tomato Plants.
- Author
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Soliman, Gazeia M., El-Sawy, Sameh M., Salim, Rasha G., El-Sayed, Ghada M., and Ramadan, Walaa A.
- Subjects
BACILLUS cereus ,SOUTHERN root-knot nematode ,GENE expression ,NEMATOCIDES ,IRRADIATION ,TOMATOES ,BIG bands - Abstract
Stress the use of safer options such as biological control to avoid the hazards of chemical nematicides. UV irradiation has been used to induce mutations and improve protease overproduction. All mutants recorded significant decreases in nematode-related parameters when compared with the wild type and control. Mutant number one (M1) achieved high protease activity about 2.5 fold. At two concentrations of the wild type Bacillus cereus and their mutants effect on percentage mortality of Meloidogyne incognita juveniles. M1 achieves the highest mortality, 99.33 %, and achieved greatest reductions in the number of juveniles in soil, juveniles/5 g root, galls, and egg-masses recorded 89.76 %, 90.16 %, 85.29 %, and 89.11 %, respectively. M1 achieved the best increase of the growth parameters of plant length, number of branches, number of leaves, and fresh weight of leaves recorded 54.35 %, 71.45 %, 169.44 %, and 256.17 %, respectively. These results are in harmony with those of leaves water soluble-protein electrophoretic patterns after one month and the end of the application, which showed that the plant exposed to M1 exhibited the highest number of bands in both stages of application and appeared unique band at M. W 55kDa after one month of application. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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