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This study delved into the spatiotemporal evolution and impact mechanisms of areca palm (Areca catechu L.) plantations in China. Using Landsat and Google Earth imagery combined with machine learning, the geographical distribution of areca palm was mapped at a 30 m resolution from 1987 to 2022, achieving an overall classification accuracy of 0.67 in 2022. The plantation area rapidly expanded from 8064 hectares in 1987 to 193,328 hectares in 2022. Spatially, there was a pronounced trend of overall agglomeration in areca palm plantations, primarily displaying two distribution patterns: high-value aggregation and low-value aggregation. Moreover, the plantation area exhibited a significant positive correlation with both GDP (r = 0.98, p < 0.001) and total population (r = 0.92, p < 0.01), while negatively correlating with rural population (r = −0.76, p < 0.05). No significant correlation was observed with environmental factors. This study elucidated the patterns and trends concerning economic development across regions and the impact of monoculture on Hainan Island's ecological environment. Comprehensive, large-scale, long-term mapping of areca palms will enhance our understanding of global agriculture's sustainability challenges and inform policy development. [ABSTRACT FROM AUTHOR]

Yellow leaf disease (YLD) poses a significant challenge to areca palm cultivation, yet its etiology remains uncertain. During our investigation of YLD-affected areca palm plants, transcriptome sequencing revealed an RNA contig exhibiting striking similarities to the RNA-dependent RNA polymerase (RdRp) of ormycoviruses. Subsequent gene cloning techniques yielded the full-length sequence of this RNA, potentially representing either the complete or partial genome of a hitherto unidentified ormycovirus, tentatively named areca palm yellow leaf-associated ormycovirus (APYLaOMV). RT-PCR detection found that APYLaOMV is present in over 30% of YLD-affected areca palm samples but is absent in healthy ones, suggesting a potential link between APYLaOMV and YLD. In summary, these data could be valuable in understanding the etiology of YLD in areca palms. [ABSTRACT FROM AUTHOR]

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Background: Areca palm (Areca catechu) is a woody perennial plant of both economical and medicinal importance grown in tropical and subtropical climates. Yet, the molecular biology study of areca palm is extremely impeded by its unavailability of a transformation method. An efficient protoplast isolation and transformation system could be highly desirable to overcome this barrier. Results: Here, we described a simple and efficient method for protoplast isolation and transformation from the perennial plant areca palm. A high yield of protoplasts (2.5 × 107 protoplasts per gram of fresh leaf tissues) was obtained from the fresh light green leaflet from the newly-emerged leaf digested overnight in the enzyme solution [2% (w/v) cellulase R10, 0.5% (w/v) macerozyme R10, 0.7 M mannitol, 10 mM CaCl2, 20 mM KCl, 20 mM MES and 0.1% (w/v) BSA, pH 5.7] by the direct leaf-peeling method. The isolated areca protoplasts maintain viability of 86.6% and have been successfully transformed with a green fluorescent protein (GFP)-tagged plasmid (pGreen0029-GFP, 6.0 kb) via the polyethylene glycol (PEG)-mediated transformation. Moreover, the mannitol concentration (optimal: 0.7 M) was determined as a key factor affecting areca protoplast isolation. We also demonstrated that the optimal density of areca protoplast for efficient transformation was at 1.0–1.5 × 106 cells/ml. With the optimization of transformation parameters, we have achieved a relatively high transformation efficiency of nearly 50%. Conclusion: We have established the first efficient protocol for the high-yield isolation and transformation of areca palm protoplasts. This method shall be applied in various biological studies of areca palm, such as gene function analysis, genome editing, protein trafficking and localization and protein–protein interaction. In addition, the protoplast system offers a great genetic transformation approach for the woody perennial plant-areca palm. Moreover, the established platform may be applied in protoplast isolation and transformation for other important species in the palm family, including oil palm and coconut. [ABSTRACT FROM AUTHOR]

Yellow leaf disease (YLD) has been a major limiting factor threatening areca palm commonly known as betel palm (Areca catechu L.) plantations in Hainan, China. The YLD disease is closely associated with areca palm velarivirus 1 (APV1), which belongs to the family Closteroviridae. YLD-affected betel palms show more serious yellowing symptoms in winter than in summer based on anecdotal observations. In the present work, the underlying mechanism was investigated. We first observed that the severity of YLD symptoms was closely related with the APV1 viral titer determined by qRT-PCR and ELISA under natural conditions. To further investigate whether temperature plays a key role in APV1 accumulation, the areca palm seedlings were artificially inoculated with APV1-positive mealybugs (Ferrisia virgata) and then cultivated under controlled conditions. According to our results, the YLD symptoms severity in inoculated seedlings were closely associated with temperature, e.g., severest symptoms at low temperature (16/22 ± 2°C, night/day), severer symptoms at room temperature (24/26 ± 2°C, night/day), while moderate symptoms at high temperature (27/34 ± 2°C, night/day). The qRT-PCR and ELISA results showed that APV1 titer accumulates significantly abundant at low temperature as compared to high and room temperatures. In conclusion, this is the first report about the temperature effects on the symptoms severity of YLD and APV1 titer, which may have important implications for the epidemiology of YLD. [ABSTRACT FROM AUTHOR]

Deterioration of indoor air quality (IAQ) has become a serious concern as people spend lots of time indoors and prolonged pollution exposure can result in adverse health outcomes. Indoor plants can phytoremediate a wide variety of indoor contaminants. Nonetheless, few experiments have demonstrated their efficacy in real-time environments. Therefore, the present study aims to experimentally assess the efficiency of Areca palm potted plants in phytoremediation of primary indoor air pollutant viz. total volatile organic compounds (TVOCs), carbon dioxide (CO2), and carbon monoxide (CO) levels from real-world indoor spaces, for the first time. Four discrete naturally ventilated experimental sites (I-IV) situated at the Council of Scientific and Industrial Research- Institute of Himalayan Bioresource Technology (CSIR-IHBT) were used. For over a period of 4 months, the sites were monitored using zero plants as a control (1–4 week), three plants (5–8 week), six plants (9–12 week), and nine plants (13–16 week), respectively. Present results indicate that Areca palm potted plants can effectively reduce the TVOCs, CO2, and CO levels by 88.16% in site IV, 52.33% and 95.70% in site III, respectively. The current study concluded that Areca palm potted plants offer an efficient, cost-effective, self-regulating, sustainable solution for improving indoor air quality and thereby human well-being and productivity in closed and confined spaces. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Plant Production is the property of Egyptian National Agricultural Library (ENAL) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)