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The study assessed the individual and interactive role of acetylsalicylic acid (ASA) and nitric oxide (NO) against excess copper (Cu) concentrations. Excess Cu significantly reduced growth and photosynthesis, although the application of ASA and NO significantly mitigated the reduction with much visible effect in their combination. The oxidative effects of Cu have been evident as increased reactive oxygen species (ROS) production, resulting in increased malondialdehyde (MDA) and reduced membrane stability index (MSI). Both ASA and NO reduced oxidative damage obvious as reduced MDA and lipoxygenase (LOX) down-regulation. Up-regulation of enzymatic and non-enzymatic antioxidants due to ASA and NO application contributed to the quick elimination of excess ROS. Oxidative effects of excess Cu were mitigated by osmolyte accumulation, up-regulation of the γ-Glutamyl kinase (γ -GK), glyoxalase, and down-regulation of proline oxidase (PROX). Reduced Cu accumulation in our study showed the crosstalk effect of ASA and NO resulting in the protection of mung bean growth against the damaging effects of Cu. [ABSTRACT FROM AUTHOR]

White button mushrooms are greatly high perishable and can deteriorate within a few days after harvesting due to physicomechanical damage, respiration, microbial growth of the delicate epidermal structure. For that reason, the present research work was applied to evaluate the effect of chitosan combination with nano-coating treatments on physicochemical parameters and microbial populations on button mushrooms at chilling storage. Nano coating with the addition of nisin 1% (CHSSN/M) established the minimum value for weight loss 12.18%, maintained firmness 11.55 N, and color index profile. Moreover, O2% rate of (CHSSN/M) mushrooms was the lowest at 1.78%; while the highest rate was reported for CO2 24.88% compared to the untreated samples (Control/M) on day 12. Both pH and total soluble solid concentrations increased during storage. Results reported that the (CHSS/M) mushroom significantly (P < 0.05) reduced polyphenol oxidase activity (24.31 U mg−1 Protein) compared with (Control/M) mushrooms that increased faster than the treated samples. (CHSSN/M) treatment was the most efficient in the reduction of yeast and mold, aerobic plate microorganisms (5.27–5.10 log CFU/g), respectively. The results established that nano-coating film might delay the aging degree and accompany by marked prolongation of postharvest mushroom freshness. [ABSTRACT FROM AUTHOR]