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The purpose of this study was to determine the agro-morphological, physiological, and transcriptional characteristics of ethyl methane sulfonate (EMS)-mutagenized M3 alfalfa (Medicago sativa L.) mutants grown for 24 days under water deficit conditions following the first cutting at the blooming bud stage. Drought stress generally reduced all agro-morphological parameters measured. Cutting stress significantly reduced total chlorophyll contents of all genotypes, with the exception of mutant X20. Significant changes occurred in the levels of ascorbate peroxidase (APX), lipid peroxidation (TBARS), glutathione reductase (GR), superoxide dismutase (SOD) and crude protein levels. Drought stress considerably elevated GR levels in irrigated control (C1) and unirrigated control (C2) plants. In contrast to the other genotypes studied, mutant X20's crude protein level increased significantly after the first cutting under 24 days of water deficit conditions, compared to the level found before cutting. The expression profiles of Medicago truncatula Response to Desiccation 2 (MtRD2) and Medicago sativa proline dehydrogenase (MsProDH) genes differed based on the plant genotype and duration of drought stress. Drought stress increased MsProDH gene expression in X20, X50, and C2 plants by 4.57, 14.8, and 20.65 times, respectively. The findings of the expression study indicated that the MtRD2 gene may play a key role in stress reduction rather than drought stress per se. The results of this study revealed that cutting and drought are two independent stress variables that should be evaluated separately when evaluating genotype response to both irrigated and unirrigated growing environments in alfalfa. [ABSTRACT FROM AUTHOR]

Copyright of Duzce University Journal of Science & Technology is the property of Duzce University Journal of Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agriculture & Nature / Kahramanmaraş Sütçü İmam Üniversitesi Tarım & Doğa Dergisi is the property of Kahramanmaras Sutcu Imam Universitesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Drought is the main abiotic stress factor that negatively affects the growth, development, and yield of plants. Salicylic acid (SA) is a plant growth regulator associated with stress tolerance in plants. Exogenous application of SA prevents against stress dependent damage. Strigolactones (SLs) are another phytohormone in plants, they are known to positively affect plant growth with exogenous applications due to their potential to stimulate the tolerance system of plants under stress conditions. The aim of this study is determine to SA and GR24 effects on the negative impacts of drought stress on tomato "Full F1" seedlings, which is the most preferred commercial variety by professional farmers in Çanakkale (Turkey), based on physiological [(shoot-root length, biomass, relative water content (RWC), specific leaf area (SLA), total chlorophyll content (SPAD)] and biochemical parameters [Total protein amount, glutathione reductase activity (GR), catalase activity (CAT), peroxidase activity (POX), ascorbate peroxidase activity (APX), hydrogen peroxide amount (H2O2), lipid peroxidation amount (TBARS)]. Fourty-five days old seedlings kept five days for acclimation, then the seedlings were treated with exogenous GR24 (0.015 mM) and SA (0.1 mM) applications. According to our results, Full F1 tomato variety was adversely affected by short-term drought stress. However, especially SA+GR24 application reduced lipid peroxidation by regulating antioxidant capacity and increased drought tolerance of this cultivar. In this context, it can be said that the combined use of these phytohormones can be used to protect the Full F1 tomato variety from drought stress damage. [ABSTRACT FROM AUTHOR]

The objectives of this study were to determine drought tolerant novel mutant of alfalfa (Medicago sativa L.) genotypes by screening EMS mutagenized 340675 M3 seeds at germination stages in the presence of osmotic stress of 35% PEG6000. Root growth assay provided several drought tolerant candidate mutants. Of those, 4 mutants were further evaluated at water deficit conditions applied for 24 days after the first cutting at flowering bud stage. The results revealed that mutants determined as drought tolerant at germination stage were also tolerant to water deficit conditions. Protein content and superoxide dismutase values were found to be higher in all mutants than controls. Ascorbate peroxides, glutton reductase and lipid peroxidase values varied based on the mutant genotype and duration of drought stress. Drought stress significantly changed transcriptional levels of MtP5CS, MtDehyd, MseIF-2, MtRD2 and MsNAC genes. These results indicated that in vitro screening of alfalfa mutant seeds for osmatic tolerance at germination and early seedling growth stages was successfully able to determine the drought tolerant alfalfa mutants which were also tolerant to water deficit conditions after the first cutting at flowering bud stage. [ABSTRACT FROM AUTHOR]

Copyright of COMU Journal of Agriculture Faculty / ÇOMÜ Ziraat Fakültesi Dergisi is the property of Canakkale Onsekiz Mart University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Turkish Journal of Weed Science is the property of Gaziosmanpasa Universitesi Matbaasi, Department of Plant Protection, Faculty of Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Anadolu Tarım Bilimleri Dergisi is the property of Ondokuz Mayis Universitesi, Ziraat Fakultesi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Extracts of medicinal plants can be used as biostimulants to overcome environmental stress conditions in cultivated plants. Herbicides are commonly used for weed control in wheat fields and accepted as abiotic stress factors for both weeds and cultivated plants. This study was aimed at determining the effects of rosehip (Rosa canina L.) fruit extract (20 %, 10 ml/pot, R) and tribenuron methyl (1 g/acre, H) on the antioxidant system in two wheat varieties (drought-tolerant Triticum aestivum cv. 'Tosunbey' and drought-sensitive cv. 'Sultan-95'). Wheat seedlings were divided into four groups: non-treated seedlings (C, Control), seedlings treated with rosehip fruit extract (R), seedlings treated with tribenuron methyl (H), and R seedlings treated with tribenuron methyl at the 28th day of the experiment (20 %, 10 ml/pot) (HR). R treatment increased root-shoot elongation in the 'Tosunbey' variety. All treatments decreased chlorophyll content in 'Tosunbey' but did not change it in 'Sultan-95'. R treatment did not alter superoxide dismutase (SOD) isoenzyme amount and peroxidase (POX) activities in 'Sultan-95' but increased lipid peroxidation (LP). However, 'Tosunbey' showed a low SOD isoenzyme amount and stable LP and cell membrane permeability (CMP). Thus, R treatment provided difference between these varieties. R treatment caused stable LP in the drought-resistant variety and increased it in drought-sensitive variety. LP did not decrease due to decrease in SOD isoenzyme amount and POX activity in 'Sultan-95' after HR treatment. However, HR treatment decreased LP and CMP and showed increased POX activity in 'Tosunbey'. Consequently, it was assumed that rosehip fruit extract treatment protects by inducing POX activity in the droughtresistant variety after herbicide treatment. [ABSTRACT FROM AUTHOR]

Broomrapes are the most difficult plant parasites to control among all biotic stresses affecting plants in the Mediterranean, Europe and Asia. Lentil is sensitive to broomrape (Orobanche crenata), which causes significant yield losses in this species around the Mediterranean region. Our study was aimed at the evaluation of antibacterial properties of Lens culinaris cv. 'Sultan-1' under drought stress and broomrape infection stress conditions. The antimicrobial activity of the ethanol extract of 'Sultan-1' was assayed against the test for microorganisms by methods of diffusion in agar and dilution in broth. The ethanol extracts from different studied treatments showed antimicrobial activities, with diameters of the inhibition zone ranging from 7 mm to 15 mm and from 2.5 to 20 µg mL-1, respectively. The highest antimicrobial activity against A. baumannii ATCC 19606 was demonstrated by the extract of 'Sultan-1' at combined stress (drought stress + broomrape infection) on the 1st day. Furthermore, the combined stress treatment promoted antibacterial effect against some Gram-negative bacteria on the 1st day. [ABSTRACT FROM AUTHOR]

Lentil (Lens culinaris) is moderately resistant to drought and high temperatures but sensitive to weeds such as broomrape (Orobanche crenata). The current study was aimed at the evaluation of the antimicrobial properties of L. culinaris 'Çiftçi' under drought and broomrape infection stresses. Antimicrobial activity of the ethanolic extract of 'Çiftçi' was was assayed against some test bacteria and yeast culture by the agar disc diffusion method and micro broth dilution method. The results suggested that the ethanol extracts from the different studied treatments showed antimicrobial activities, with the diameters of the inhibition zone ranging from 7.0 to 13.0 mm and 5.0 to 20.0 µg/mL, respectively. The most intensive antimicrobial activity against Proteus vulgaris ATCC 13315 was demonstrated by the extract of 'Çiftçi' on the 7th day grown under drought. However, only the combined stress (drought and broomrape infection) treatment has promoted antagonistic effects of the extract against Staphylococcus aureus ATCC 25923 bacteria, as compared to the control on the 7th day. [ABSTRACT FROM AUTHOR]

Copyright of Biologica Nyssana is the property of University of Nis, Department of Biology & Ecology, Faculty of Sciences & Mathematics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Examines the microbiological features of aminotransferase enzyme (AST)-positive periodontal sites. Components of gingival crevicular fluid; Assessment of AST levels; Suggestion to further evaluate the ability of the AST test to differentiate the microbial flora of progressing sites.

Copyright of Journal of Clinical Periodontology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)