19 results on '"H. Peters"'
Search Results
2. From clusters to crystals: scale chemistry of intermetallics.
- Author
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Shevchenko, Vladimir Ya., Medrish, Inna V., Ilyushin, Gregory D., and Blatov, Vladislav A.
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COMPUTER algorithms ,CRYSTALS ,CRYSTAL structure ,CHEMISTRY - Abstract
We review different approaches to description, classification, and understanding of intermetallic structures of any complexity. All these approaches are based on the representation of the intermetallic structure as a periodic graph (net). A special attention is paid to the nanocluster model, which enables one to separate structural units and to describe their assemblage in intermetallics with strict computer algorithms. We demonstrate the abilities of the model with analysis of 12,315 intermetallic structures from the Inorganic Crystal Structure Database. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
3. How does the content of nutrients in soil affect the health status of trees in city parks?
- Author
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Kleiber, Tomasz, Krzyżaniak, Michał, Świerk, Dariusz, Haenel, Anna, and Gałecka, Sylwia
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URBAN trees ,FISHER discriminant analysis ,CHESTNUT ,URBAN parks ,SOIL composition - Abstract
Trees have multi-aspect influence on the microclimate in urbanised areas. Therefore, it is important to investigate the biotic and abiotic factors affecting their health. The aim of the conducted study was to assess the chemical composition of soils and the nutritional status of lime and horse chestnut trees in selected sites and the influence of these factors on the condition and health of these tree species in urbanised areas. The research was conducted on selected trees (n = 643) growing in different parts of the city. The soils and plants were analysed for the content of macro- and microelements, sodium and heavy metals. A canonical variation analysis (CVA)–the canonical variant of Fisher's linear discriminant analysis (LDA) was used to construct the model. The CVA enabled the creation of 4 CCA models. The research showed that in general, the soil in all the sites of lime and horse chestnut trees was alkalised–at the same time it was characterised by low salinity. Despite the alkaline soil the statistical analysis showed a positive correlation between the content of manganese in the lime leaves and the deterioration of their health. In spite of that due to the satisfactory health status and condition of trees in most locations temporary guide values of nutrients were proposed for trees growing in urbanised areas. The following temporary guide values of nutrients were proposed for the horse chestnut trees (% d. m.): N 2.38%-4.71%, P 0.24%-0.46%, K 1.13%-2.31%, Ca 1.05%-2.12%, Mg 0.16%-0.42%, S 0.12%-0.23%; Fe 89.8–198.8, Zn 17.6–33.1, Cu 7.36–19.61 (mg kg
-1 d. m.). The following temporary guide values were proposed for the small-leaved lime-trees (% d. m.): N 2.45%-3.22%, P 0.27%-0.42%, K 1.52%-2.86%, Ca 1.43%-2.02%, Mg 0.19%-0.35%, S 0.19%-0.25%; Fe 137.6–174.3, Zn 20.2–23.8, Cu 8.36–9.79 (mg kg-1 d. m.). [ABSTRACT FROM AUTHOR]- Published
- 2019
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4. Hormonal contraceptive use and Staphylococcus aureus nasal and throat carriage in a Norwegian youth population.
- Author
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Stensen, Dina B., Småbrekke, Lars, Olsen, Karina, Grimnes, Guri, Nielsen, Christopher Sivert, Simonsen, Gunnar Skov, Sollid, Johanna U. E., and Furberg, Anne-Sofie
- Subjects
DEMOGRAPHIC surveys ,STAPHYLOCOCCUS aureus ,METHICILLIN-resistant staphylococcus aureus ,YOUTH ,ADOLESCENCE - Abstract
Background: Use of hormonal contraceptives has been associated with Staphylococcus aureus nasal carriage in adult women. However, the role of hormonal contraceptives in S. aureus colonization among adolescents and associations with progestin only contraceptives are unknown. Methods: We obtained nasal and throat swab samples from 439 girls aged 17–21 years in the population-based Tromsø study Fit Futures, 2012–2013, Norway, with information on lifestyle, health and biomarkers. We used multivariable logistic regression to study the association between use of hormonal contraceptives and Staphylococcus aureus carriage while adjusting for potential confounding factors. Results: Staphylococcus aureus nasal carriage prevalence were 34%, 42%, and 61% among progestin-only users, non-users, and progestin-estrogen combination contraceptive users, respectively (P<0.001). Use of combination contraceptives doubled the odds of nasal carriage (non-users reference; OR = 2.31, 95%CI = 1.43–3.74). The OR of nasal carriage was 0.29 among progestin-only users compared to combination contraceptives users (95% CI = 0.12–0.67). Discussion: In this study, use of combination hormonal contraceptives was associated with higher risk of Staphylococcus aureus nasal carriage in adolescent girls. Experimental design studies are needed to establish the role of exogenous sex steroids in Staphylococcus aureus colonization in women. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
5. Rosiglitazone in the thawing medium improves mitochondrial function in stallion spermatozoa through regulating Akt phosphorylation and reduction of caspase 3.
- Author
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Ortiz-Rodriguez, José M., Balao da Silva, Carolina, Masot, Javier, Redondo, Eloy, Gazquez, Antonio, Tapia, José A., Gil, Cruz, Ortega-Ferrusola, Cristina, and Peña, Fernando J.
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SPERMATOZOA ,FROZEN semen ,STALLIONS ,ROSIGLITAZONE ,OXIDATIVE phosphorylation ,PHOSPHORYLATION ,CYTOLOGY - Abstract
Background: The population of stallion spermatozoa that survive thawing experience compromised mitochondrial functionality and accelerated senescence, among other changes. It is known that stallion spermatozoa show very active oxidative phosphorylation that may accelerate sperm senescence through increased production of reactive oxygen species. Rosiglitazone has been proven to enhance the glycolytic capability of stallion spermatozoa maintained at ambient temperature. Objectives: Thus, we hypothesized that thawed sperm may also benefit from rosiglitazone supplementation. Materials and methods: Thawed sperm were washed and resuspended in Tyrodes media, and the samples were divided and supplemented with 0 or 75 μM rosiglitazone. After one and two hours of incubation, mitochondrial functionality, Akt phosphorylation and caspase 3 activity were evaluated. Additional samples were incubated in the presence of an Akt1/2 inhibitor, compound C (an AMPK inhibitor) or GW9662 (an antagonist of the PPARγ receptor). Results: Rosiglitazone maintained Akt phosphorylation and reduced caspase 3 activation (p<0.01), both of which were prevented by incubation in the presence of the three inhibitors. Rosiglitazone also enhanced mitochondrial functionality (P<0.01). Conclusion: We provide the first evidence that the functionality of frozen stallion spermatozoa can be potentially improved after thawing through the activation of pro survival pathways, providing new clues for improving current sperm biotechnology. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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6. Phenotypic selection with an intrabody library reveals an anti-apoptotic function of PKM2 requiring Mitofusin-1.
- Author
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Liu, Tong, Kuwana, Tomomi, Zhang, Hongkai, Vander Heiden, Matthew G., Lerner, Richard A., and Newmeyer, Donald D.
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PHENOTYPES ,MITOFUSIN 1 protein ,BCL-2 proteins ,CANCER cells ,CANCER - Abstract
Bcl-2 family proteins control a decisive apoptotic event: mitochondrial outer membrane permeabilization (MOMP). To discover MOMP-regulating proteins, we expressed a library of intracellular single-chain variable fragments (scFvs) (“intrabodies”) and selected for those rescuing cells from apoptosis induced by BimS (the short isoform of Bim). One anti-apoptotic intrabody, intrabody 5 (IB5), recognized pyruvate kinase M2 (PKM2), which is expressed in cancer cells. PKM2 deletion ablated this clonogenic rescue; thus, IB5 activated a latent cytoprotective function of PKM2. This resulted not from pyruvate kinase activity per se but rather from the formation of an active tetrameric conformation of PKM2. A stably tetrameric PKM2 mutant, K422R, promoted cell survival even in the absence of IB5, and IB5 further increased survival. Mitochondria isolated from IB5-expressing cells were relatively resistant to MOMP in vitro. In cells, IB5 expression up-regulated Mitofusin-1 (Mfn1) and increased mitochondrial length. Importantly, Mfn1 deficiency abrogated IB5’s cytoprotective effect. PKM2’s anti-apoptotic function could help explain its preferential expression in human cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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7. Poor extraction efficiencies of polystyrene nano- and microplastics from biosolids and soil.
- Author
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Wang, Zhan, Taylor, Stephen E., Sharma, Prabhakar, and Flury, Markus
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SEDIMENTS ,POLYSTYRENE ,SEWAGE sludge ,ORGANIC compounds ,EXTRACTION (Chemistry) - Abstract
Extraction and quantification of nano- and microplastics from sediments and soils is challenging. Although no standard method has been established so far, flotation is commonly used to separate plastic from mineral material. The objective of this study was to test the efficiency of flotation for the extraction of nano- and microplastics from biosolids and soil. We spiked biosolids and soil samples with polystyrene nano- and microbeads (0.05, 1.0, 2.6, 4.8, and 100 μm diameter). Different extraction methods (w/ and w/o H
2 O2 digestion) were tested, and plastic beads were separated from mineral particles by flotation in a ZnCl2 solution. Plastic particles were quantified by UV-Vis spectrometry and gravimetrically. While large beads (100 μm) could be quantitatively extracted (∼100%) from both biosolids and soils, smaller beads had low extraction efficiencies (ranging from 5 to 80%, with an average of 20%). Except for the 100 μm beads, oxidation with H2 O2 negatively impacted the extraction efficiencies. For the soil, extraction with water only, followed by flotation in a ZnCl2 solution, resulted in relatively high extraction efficiencies (>75%) for beads larger than 1 μm, but low efficiencies (<30%) for the 0.05 and 1.0 μm beads. Our results indicate that while flotation generally works to separate plastic nano- and microbeads in a solution, the challenge is to quantitatively extract nano- and microbeads from a biosolids or soil matrix. Samples high in organic matter content require removal of the organic matter, but the common method of H2 O2 oxidation leads to poor extraction efficiencies for nano- and microbeads. [ABSTRACT FROM AUTHOR]- Published
- 2018
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8. Global analysis of genetic circuitry and adaptive mechanisms enabling resistance to the azole antifungal drugs.
- Author
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Mount, Harley O’Connor, Revie, Nicole M., Todd, Robert T., Anstett, Kaitlin, Collins, Cathy, Costanzo, Michael, Boone, Charles, Robbins, Nicole, Selmecki, Anna, and Cowen, Leah E.
- Subjects
ANTIFUNGAL agents ,GENOMES ,PATHOGENIC microorganisms ,CANDIDA albicans ,GENE expression ,AZOLES ,TETRAZOLES - Abstract
Invasive fungal infections caused by the pathogen Candida albicans have transitioned from a rare curiosity to a major cause of human mortality. This is in part due to the emergence of resistance to the limited number of antifungals available to treat fungal infections. Azoles function by targeting the biosynthesis of ergosterol, a key component of the fungal cell membrane. Loss-of-function mutations in the ergosterol biosynthetic gene ERG3 mitigate azole toxicity and enable resistance that depends upon fungal stress responses. Here, we performed a genome-wide synthetic genetic array screen in Saccharomyces cerevisiae to map ERG3 genetic interactors and uncover novel circuitry important for azole resistance. We identified nine genes that enabled erg3-mediated azole resistance in the model yeast and found that only two of these genes had a conserved impact on resistance in C. albicans. Further, we screened a C. albicans homozygous deletion mutant library and identified 13 genes for which deletion enhances azole susceptibility. Two of the genes, RGD1 and PEP8, were also important for azole resistance acquired by diverse mechanisms. We discovered that loss of function of retrograde transport protein Pep8 overwhelms the functional capacity of the stress response regulator calcineurin, thereby abrogating azole resistance. To identify the mechanism through which the GTPase activator protein Rgd1 enables azole resistance, we selected for mutations that restore resistance in strains lacking Rgd1. Whole genome sequencing uncovered parallel adaptive mechanisms involving amplification of both chromosome 7 and a large segment of chromosome 3. Overexpression of a transporter gene on the right portion of chromosome 3, NPR2, was sufficient to enable azole resistance in the absence of Rgd1. Thus, we establish a novel mechanism of adaptation to drug-induced stress, define genetic circuitry underpinning azole resistance, and illustrate divergence in resistance circuitry over evolutionary time. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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9. Comprehensive analysis of central carbon metabolism illuminates connections between nutrient availability, growth rate, and cell morphology in Escherichia coli.
- Author
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Westfall, Corey S. and Levin, Petra Anne
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CARBON metabolism ,CELL morphology ,ESCHERICHIA coli ,GAMETOGENESIS ,POLLEN dispersal ,NUCLEOTIDE synthesis ,CYCLIC-AMP-dependent protein kinase genetics - Abstract
Bacterial morphology is a complex trait that is highly sensitive to changes in the environment. For heterotrophic organisms, such as Escherichia coli, increases in nutrient levels are frequently accompanied by several-fold increases in both size and growth rate. Despite the dramatic nature of these changes, how alterations in nutrient availability translate into changes in growth and morphology remains a largely open question. To understand the signaling networks coupling nutrient availability with size and shape, we examined the impact of deletions in the entirety of non-essential central carbon metabolic genes on E. coli growth rate and cell size. Our data reveal the presence of multiple metabolic nodes that play important yet distinctive roles in dictating biosynthetic capacity and shaping cell morphology. Specifically, perturbations of acetyl-CoA metabolism impact cell size and division through changes in fatty acid synthesis. Additionally, we identify a genetic pathway linking glucose levels to cell width through the signaling molecule cyclic-AMP. Together our findings highlight a surprising diversity of factors and mechanisms contributing to growth potential and cell morphology, providing a foundation for further studies. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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10. A nonrandomized trial of vitamin D supplementation for Barrett’s esophagus.
- Author
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Cummings, Linda C., Thota, Prashanthi N., Willis, Joseph E., Chen, Yanwen, Cooper, Gregory S., Furey, Nancy, Bednarchik, Beth, Alashkar, Bronia M., Dumot, John, Faulx, Ashley L., Fink, Stephen P., Kresak, Adam M., Abusneineh, Basel, Barnholtz-Sloan, Jill, Leahy, Patrick, Veigl, Martina L., Chak, Amitabh, and Markowitz, Sanford D.
- Subjects
ESOPHAGEAL cancer patients ,APOPTOSIS ,VITAMIN D deficiency ,GENE expression in mammals ,BIOPSY ,GENETICS ,VITAMIN deficiency - Abstract
Background: Vitamin D deficiency may increase esophageal cancer risk. Vitamin D affects genes regulating proliferation, apoptosis, and differentiation and induces the tumor suppressor 15-hydroxyprostaglandin dehydrogenase (PGDH) in other cancers. This nonrandomized interventional study assessed effects of vitamin D supplementation in Barrett’s esophagus (BE). We hypothesized that vitamin D supplementation may have beneficial effects on gene expression including 15-PGDH in BE. Methods: BE subjects with low grade or no dysplasia received vitamin D
3 (cholecalciferol) 50,000 international units weekly plus a proton pump inhibitor for 12 weeks. Esophageal biopsies from normal plus metaplastic BE epithelium and blood samples were obtained before and after vitamin D supplementation. Serum 25-hydroxyvitamin D was measured to characterize vitamin D status. Esophageal gene expression was assessed using microarrays. Results: 18 study subjects were evaluated. The baseline mean serum 25-hydroxyvitamin D level was 27 ng/mL (normal ≥30 ng/mL). After vitamin D supplementation, 25-hydroxyvitamin D levels rose significantly (median increase of 31.6 ng/mL, p<0.001). There were no significant changes in gene expression from esophageal squamous or Barrett’s epithelium including 15-PGDH after supplementation. Conclusion: BE subjects were vitamin D insufficient. Despite improved vitamin D status with supplementation, no significant alterations in gene expression profiles were noted. If vitamin D supplementation benefits BE, a longer duration or higher dose of supplementation may be needed. [ABSTRACT FROM AUTHOR]- Published
- 2017
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11. Live-cell imaging to measure BAX recruitment kinetics to mitochondria during apoptosis.
- Author
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Maes, Margaret E., Schlamp, Cassandra L., and Nickells, Robert W.
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CELL imaging ,MITOCHONDRIA ,OLIGOMERS ,APOPTOSIS ,CHIMERIC proteins - Abstract
The pro-apoptotic BCL2 gene family member, BAX, plays a pivotal role in the intrinsic apoptotic pathway. Under cellular stress, BAX recruitment to the mitochondria occurs when activated BAX forms dimers, then oligomers, to initiate mitochondria outer membrane permeabilization (MOMP), a process critical for apoptotic progression. The activation and recruitment of BAX to form oligomers has been studied for two decades using fusion proteins with a fluorescent reporter attached in-frame to the BAX N-terminus. We applied high-speed live cell imaging to monitor the recruitment of BAX fusion proteins in dying cells. Data from time-lapse imaging was validated against the activity of endogenous BAX in cells, and analyzed using sigmoid mathematical functions to obtain detail of the kinetic parameters of the recruitment process at individual mitochondrial foci. BAX fusion proteins behave like endogenous BAX during apoptosis. Kinetic studies show that fusion protein recruitment is also minimally affected in cells lacking endogenous BAK or BAX genes, but that the kinetics are moderately, but significantly, different with different fluorescent tags in the fusion constructs. In experiments testing BAX recruitment in 3 different cell lines, our results show that regardless of cell type, once activated, BAX recruitment initiates simultaneously within a cell, but exhibits varying rates of recruitment at individual mitochondrial foci. Very early during BAX recruitment, pro-apoptotic molecules are released in the process of MOMP, but different molecules are released at different times and rates relative to the time of BAX recruitment initiation. These results provide a method for BAX kinetic analysis in living cells and yield greater detail of multiple characteristics of BAX-induced MOMP in living cells that were initially observed in cell free studies. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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12. Staphylococcus aureus SrrAB Affects Susceptibility to Hydrogen Peroxide and Co-Existence with Streptococcus sanguinis.
- Author
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Oogai, Yuichi, Kawada-Matsuo, Miki, and Komatsuzawa, Hitoshi
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STAPHYLOCOCCUS aureus ,HYDROGEN peroxide ,STREPTOCOCCUS sanguis ,ANTI-infective agents ,NITRIC oxide - Abstract
Staphylococcus aureus is a pathogen and a commensal bacterial species that is found in humans. Bacterial two-component systems (TCSs) sense and respond to environmental stresses, which include antimicrobial agents produced by other bacteria. In this study, we analyzed the relation between the TCS SrrAB and susceptibility to the hydrogen peroxide (H
2 O2 ) that is produced by Streptococcus sanguinis, which is a commensal oral streptococcus. An srrA-inactivated S. aureus mutant demonstrated low susceptibility to the H2 O2 produced by S. sanguinis. We investigated the expression of anti-oxidant factors in the mutant. The expression of katA in the mutant was significantly higher than in the wild-type (WT) in the presence or absence of 0.4 mM H2 O2 . The expression of dps in the mutant was significantly increased compared with the WT in the presence of H2 O2 but not in the absence of H2 O2 . A katA or a dps-inactivated mutant had high susceptibility to H2 O2 compared with WT. In addition, we found that the nitric oxide detoxification protein (flavohemoglobin: Hmp), which is regulated by SrrAB, was related to H2 O2 susceptibility. The hmp-inactivated mutant had slightly lower susceptibility to the H2 O2 produced by S. sanguinis than did WT. When a srrA-inactivated mutant or the WT were co-cultured with S. sanguinis, the population percentage of the mutant was significantly higher than the WT. In conclusion, SrrAB regulates katA, dps and hmp expression and affects H2 O2 susceptibility. Our findings suggest that SrrAB is related in vivo to the co-existence of S. aureus with S. sanguinis. [ABSTRACT FROM AUTHOR]- Published
- 2016
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13. Changes in the Cytoplasmic Composition of Amino Acids and Proteins Observed in Staphylococcus aureus during Growth under Variable Growth Conditions Representative of the Human Wound Site.
- Author
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Alreshidi, Mousa M., Dunstan, R. Hugh, Gottfries, Johan, Macdonald, Margaret M., Crompton, Marcus J., Ang, Ching-Seng, Williamson, Nicholas A., and Roberts, Tim K.
- Subjects
CYTOPLASM ,AMINO acids ,STAPHYLOCOCCUS aureus ,RIBOSOMAL proteins ,HOMEOSTASIS - Abstract
Staphylococcus aureus is an opportunistic pathogen responsible for a high proportion of nosocomial infections. This study was conducted to assess the bacterial responses in the cytoplasmic composition of amino acids and ribosomal proteins under various environmental conditions designed to mimic those on the human skin or within a wound site: pH6-8, temperature 35–37°C, and additional 0–5% NaCl. It was found that each set of environmental conditions elicited substantial adjustments in cytoplasmic levels of glutamic acid, aspartic acid, proline, alanine and glycine (P< 0.05). These alterations generated characteristic amino acid profiles assessed by principle component analysis (PCA). Substantial alterations in cytoplasmic amino acid and protein composition occurred during growth under conditions of higher salinity stress implemented via additional levels of NaCl in the growth medium. The cells responded to additional NaCl at pH 6 by reducing levels of ribosomal proteins, whereas at pH 8 there was an upregulation of ribosomal proteins compared with the reference control. The levels of two ribosomal proteins, L32 and S19, remained constant across all experimental conditions. The data supported the hypothesis that the bacterium was continually responding to the dynamic environment by modifying the proteome and optimising metabolic homeostasis. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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14. The Effects of High Level Magnesium Dialysis/Substitution Fluid on Magnesium Homeostasis under Regional Citrate Anticoagulation in Critically Ill.
- Author
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Zakharchenko, Mychajlo, Los, Ferdinand, Brodska, Helena, and Balik, Martin
- Subjects
PHYSIOLOGICAL effects of magnesium ,HOMEOSTASIS ,DIALYSIS (Chemistry) ,CITRATES ,ANTICOAGULANTS ,BLOOD flow measurement ,CLINICAL trials - Abstract
Background: The requirements for magnesium (Mg) supplementation increase under regional citrate anticoagulation (RCA) because citrate acts by chelation of bivalent cations within the blood circuit. The level of magnesium in commercially available fluids for continuous renal replacement therapy (CRRT) may not be sufficient to prevent hypomagnesemia. Methods: Patients (n = 45) on CRRT (2,000 ml/h, blood flow (Qb) 100 ml/min) with RCA modality (4% trisodium citrate) using calcium free fluid with 0.75 mmol/l of Mg with additional magnesium substitution were observed after switch to the calcium-free fluid with magnesium concentration of 1.50 mmol/l (n = 42) and no extra magnesium replenishment. All patients had renal indications for CRRT, were treated with the same devices, filters and the same postfilter ionized calcium endpoint (<0.4 mmol/l) of prefilter citrate dosage. Under the high level Mg fluid the Qb, dosages of citrate and CRRT were consequently escalated in 9h steps to test various settings. Results: Median balance of Mg was -0.91 (-1.18 to -0.53) mmol/h with Mg 0.75 mmol/l and 0.2 (0.06–0.35) mmol/h when fluid with Mg 1.50 mmol/l was used. It was close to zero (0.02 (-0.12–0.18) mmol/h) with higher blood flow and dosage of citrate, increased again to 0.15 (-0.11–0.25) mmol/h with 3,000 ml/h of high magnesium containing fluid (p<0.001). The arterial levels of Mg were mildly increased after the change for high level magnesium containing fluid (p<0.01). Conclusions: Compared to ordinary dialysis fluid the mildly hypermagnesemic fluid provided even balances and adequate levels within ordinary configurations of CRRT with RCA and without a need for extra magnesium replenishment. Trial Registration: ClinicalTrials.gov Identifier: [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
15. Transient Receptor Potential Melastatin-3 (TRPM3) Mediates Nociceptive-Like Responses in Hydra vulgaris.
- Author
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Malafoglia, Valentina, Traversetti, Lorenzo, Del Grosso, Floriano, Scalici, Massimiliano, Lauro, Filomena, Russo, Valeria, Persichini, Tiziana, Salvemini, Daniela, Mollace, Vincenzo, Fini, Massimo, Raffaeli, William, Muscoli, Carolina, and Colasanti, Marco
- Subjects
TRP channels ,HYDRIDAE ,SOMATOSENSORY cortex ,HEAT shock proteins ,NITRIC-oxide synthases - Abstract
The ability of mammals to feel noxious stimuli lies in a heterogeneous group of primary somatosensory neurons termed nociceptors, which express specific membrane receptors, such as the Transient Receptor Potential (TRP) family. Here, we show that one of the most important nociceptive-like pathways is conserved in the freshwater coelenterate Hydra vulgaris, the most primitive organism possessing a nervous system. In particular, we found that H. vulgaris expresses TRPM3, a nociceptor calcium channel involved in the detection of noxious heat in mammals. Furthermore, we detected that both heat shock and TRPM3 specific agonist (i.e., pregnenolone sulfate) induce the modulation of the heat shock protein 70 (HSP70) and the nitric oxide synthase (NOS), two genes activated by TRP-mediated heat painful stimuli in mammals. As expected, these effects are inhibited by a TRPM3 antagonist (i.e., mefenamic acid). Interestingly, the TRPM3 agonist and heat shock also induce the expression of nuclear transcription erythroid 2-related factor (Nrf2) and superoxide dismutase (SOD), known markers of oxidative stress; noteworthy gene expression was also inhibited by the TRPM3 antagonist. As a whole, our results demonstrate the presence of conserved molecular oxidative/nociceptive-like pathways at the primordial level of the animal kingdom. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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16. Tin Oxide Chemistry from the Last Decade of the Nineteenth Century to the First Decade of the Twenty-First Century: Towards the Development of a Big-Picture Approach to the Teaching and Learning of Chemistry While Focussing on a Specific Compound or Class of Compounds
- Author
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de Berg, Kevin C.
- Subjects
CHEMISTRY ,TWENTIETH century ,REVOLUTIONS ,THEORY of knowledge ,STRUCTURAL frames ,TIN ,OXIDES ,QUESTIONS & answers ,SOCIALISM & society - Abstract
The discovery of the electron in 1897 deeply impacted the nature of chemistry in the twentieth century. A revolution in the theoretical structure of chemistry as well as in the instrumental tools used in chemical analysis occurred as a result of this discovery. The impact of this revolution on tin oxide chemistry over approximately a 100 year period is the focus of this paper. Important questions addressed include the following: Are tin oxides of importance in our current society or are they only of quaint academic interest? In what way has chemistry as a discipline added to our understanding of the tin oxides and has this added any technological value to these compounds? Does the historical approach followed in this paper offer chemistry educators the opportunity to place current chemical knowledge in a broader epistemological framework? The impact of the answers to these questions on chemistry education is discussed. [ABSTRACT FROM AUTHOR]
- Published
- 2010
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17. A study on the relationship between mass concentrations, chemistry and number size distribution of urban fine aerosols in Milan, Barcelona and London.
- Author
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Rodríguez, S., Van Dingenen, R., Putaud, J.-P., Dell'Acqua, A., Pey, J., Querol, X., Alastuey, A., Chenery, S., Ho, K.-F., Harrison, R., Tardivo, R., Scarnato, B., and Gemelli, V.
- Subjects
PARTICLE size distribution ,CHEMISTRY ,AEROSOLS ,EMISSIONS (Air pollution) ,URBAN pollution ,AIR pollution ,CITIES & towns - Abstract
A physicochemical characterization, including aerosol number size distribution, chemical composition and mass concentrations, of the urban fine aerosol captured in MILAN, BARCELONA and LONDON is presented in this article. The objective is to obtain a comprehensive picture of the microphysical processes involved in aerosol dynamics during the: 1) regular evolution of the urban aerosol (daily, weekly and seasonal basis) and in the day-to-day variations (from clean-air to pollution-events), and 2) the link between "aerosol chemistry and mass concentrations" with the "number size distribution". The mass concentrations of the line PM
2.5 aerosol exhibit a high correlation with the number concentration of > 100nm particles N> 100 (nm/ ("accumulation mode particles") which only account for <20% of the total number concentration N of fine aerosols; but do not correlate with the number of < 100 nm particles ("ultrafine particles"), which accounts for >80% of fine particles number concentration. Organic matter and black-carbon are the only aerosol components showing a significant correlation with the ultrafine particles, attributed to vehicles exhausts emissions; whereas ammonium-nitrate, ammonium-sulphate and also organic matter and black-carbon correlate with N>100 (nm) and attributed to condensation mechanisms, other particle growth processes and some primary emissions. Time series of the aerosol DpN diameter (dN/dlogD mode), mass PM2.5 concentrations and number N> 100 (nm) concentrations exhibit correlated day to-day variations, which point to a significant involvement of condensation of semi-volatile compounds during urban pollution events. This agrees with the observation that ammonium-nitrate is the component exhibiting the highest increases from mid-to-high pollution episodes, when the highest DpN increases arc observed. The results indicates that "fine PM2.5 particles urban pollution events" tend to occur when condensation processes have made particles grow large enough to produce significant number concentrations of N> 100 (nm) ("accumulation mode particles"). In contrast, because the low contribution of utrafine particles to the line aerosol mass concentrations, high "utrafine panicles N< 100(nm) events" frequently occurs under low PM2.5 conditions. The results of this study demonstrate that vehicles exhausts emissions are strongly involved in this ultrafine particles aerosol pollution. [ABSTRACT FROM AUTHOR]- Published
- 2007
18. Annual Review of Cosmetic Ingredient Safety Assessments-2002/2003.
- Subjects
COSMETICS ,CHEMISTRY ,PRODUCT safety ,ASSOCIATIONS, institutions, etc. ,PRODUCT liability - Abstract
Discusses the annual review of cosmetic ingredient safety assessments for 2002/2003. Assessment of the safety of cosmetic ingredients by the Cosmetic Ingredient Review (CIR) program Expert Panel; Re-review process of the CIR Expert Panel; Ingredients the CIR Expert Panel reconsidered in 2002/2003.
- Published
- 2005
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- View/download PDF
19. Microalloyed Steels through History until 2018: Review of Chemical Composition, Processing and Hydrogen Service.
- Author
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Villalobos, Julio C., Del-Pozo, Adrian, Serna, Sergio, Campillo, Bernardo, and Mayen, Jan
- Subjects
STEEL analysis ,MICROALLOYING ,METALLURGY ,MECHANICAL properties of metals ,CHEMISTRY ,FERRITES ,PEARLITIC steel - Abstract
Microalloyed steels have evolved in terms of their chemical composition, processing, and metallurgical characteristics since the beginning of the 20th century in the function of fabrication costs and mechanical properties required to obtain high-performance materials needed to accommodate for the growing demands of gas and hydrocarbons transport. As a result of this, microalloyed steels present a good combination of high strength and ductility obtained through the addition of microalloying elements, thermomechanical processing, and controlled cooling, processes capable of producing complex microstructures that improve the mechanical properties of steels. These controlled microstructures can be severely affected and result in catastrophic failures, due to the atomic hydrogen diffusion that occurs during the corrosion process of pipeline steel. Recently, a martensite–bainite microstructure with acicular ferrite has been chosen as a viable candidate to be used in environments with the presence of hydrogen. The aim of this review is to summarize the main changes of chemical composition, processing techniques, and the evolution of the mechanical properties throughout recent history on the use of microalloying in high strength low alloy steels, as well as the effects of hydrogen in newly created pipelines, examining the causes behind the mechanisms of hydrogen embrittlement in these steels. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
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