[Objective] Thermal, light, and sound environment tests and simulation experiments are an important part of the experimental teaching of building physics. However, most current experimental projects are old in content, and the influence of sound and light environment is often ignored in comfort evaluation. Therefore, students' active participation in learning and the overall coordination of indoor comfort experiments are not ideal. To solve the aforementioned problems, this paper uses human factor technology to evaluate the light and thermal environment of the subjects in the learning state from the perspective of learning efficiency and comfort. Through this teaching experiment, students can learn the design and data analysis method of orthogonal experiments and master the basic theoretical knowledge of building thermal optics, the use of relevant experimental instruments, and the measurement method of indoor light and thermal environment parameters. Students are guided to experience different light and thermal environments, and physiological parameters are used to explore their influence on comfort and learning efficiency. Finally, the best light and thermal environment for comfort and learning efficiency is comprehensively proposed. [Methods] In this paper, the evaluation methods of traditional learning performance index and four physiological parameters on learning efficiency, including heart rate variability, brainwave α rhythm engagement, cognitive engagement, and pupil diameter, were studied. The orthogonal experimental conditions of L9 (34) with four factors of temperature, humidity, illumination, and color temperature and three levels were set. The experimental results were analyzed by a range analysis method. An indoor thermal comfort tester and an illuminometer were used to collect light and thermal environment data. Among the four physiological parameters, cognitive engagement was measured by the n-back cognitive task, whereas the others were measured by the human factor data acquisition instrument. After conducting pre-experiment testing on the feasibility of the experimental scheme, students can be organized to conduct experimental teaching in small groups. The whole teaching experiment can be divided into two parts, namely, experiment preparation and formal experiment. The preparation process included laboratory explanation and instrument wearing, whereas the formal experiment included environmental adaptation, cognitive tasks, and physiological measurements in nine working conditions. Since this experiment belongs to experimental teaching, it should be mainly guided by teachers to conduct independent experiments. Teachers should fully explain and properly demonstrate operations in the preparation stage of the experiment to ensure that students can independently and smoothly carry out the experiment under the condition of the teacher's help in switching working conditions. After the completion of the experiment, the recorded experimental data were analyzed, the relationship between comfort and indoor photothermal environment and the relationship between learning efficiency and indoor photothermal environment were obtained, and the conclusion of the best indoor light and thermal environment was obtained. [Results] The experimental results showed the following: 1) The temperature approached 22 °C, the humidity approached 40%, and the illuminance approached 300 Lx. The subjects were most comfortable when the color temperature approached 3 300 K. 2) The temperature approached 18 °C, the humidity approached 40%, and the illuminance approached 500 Lx. When the color temperature approaches 5 300 K, the learning efficiency is at its highest. [Conclusions] The subjects' learning efficiency is higher in the cold light environment with lower temperature and brighter light, and they are more relaxed in the warm light environment with appropriate temperature and dimmer light. At the same time, for different learning situations, the emphasis on light and thermal environments is also different. For example, in a brighter environment (i.e., illuminance approaching 750 Lx), subjects pay more attention; subjects were less likely to fatigue under neutral light (i.e., color temperature approaching 4 500 K). [ABSTRACT FROM AUTHOR]