Search

This paper describes a new, physically interpretable, fully automatic algorithm for removal of tissue autofluorescence (AF) from fluorescence microscopy images, by non-negative matrix factorization. Measurement of signal intensities from the concentration of certain fluorescent reporter molecules at each location within a sample of biological tissue is confounded by fluorescence produced by the tissue itself (autofluorescence). Spectral mixing models use mixing coefficients to specify how much fluorescence from each source is present and unmixing algorithms separate the two fluorescent sources. Current spectral unmixing methods for AF removal often require a priori knowledge of mixing coefficients. Those which do not, such as principal component analysis, generate negative mixing coefficients that are not physically meaningful. Non-negative matrix factorization constrains mixing coefficients to be non-negative, and has been used for spectral unmixing, but not AF removal. This paper describes a novel non-negative matrix factorization algorithm which separates fluorescent images into true signal and AF components utilizing an estimate of the dark current. We also present a test-bed, based on fluorescent beads, to compare the performance of different AF removal algorithms. Our algorithm out-performed previous state of the art on validation images. [ABSTRACT FROM PUBLISHER]

Image formation algorithms in a variety of applications have explicit or implicit dependence on a mathematical model of the observation process. Inaccuracies in the observation model may cause various degradations and artifacts in the reconstructed images. The application of interest in this paper is synthetic aperture radar (SAR) imaging, which particularly suffers from motion-induced model errors. These types of errors result in phase errors in SAR data, which cause defocusing of the reconstructed images. Particularly focusing on imaging of fields that admit a sparse representation, we propose a sparsity-driven method for joint SAR imaging and phase error correction. Phase error correction is performed during the image formation process. The problem is set up as an optimization problem in a nonquadratic regularization-based framework. The method involves an iterative algorithm, where each iteration of which consists of consecutive steps of image formation and model error correction. Experimental results show the effectiveness of the approach for various types of phase errors, as well as the improvements that it provides over existing techniques for model error compensation in SAR. [ABSTRACT FROM AUTHOR]

Most existing nonblind image deblurring methods assume that the blur kernel is free of error. However, it is often unavoidable in practice that the input blur kernel is erroneous to some extent. Sometimes, the error could be severe, e.g., for images degraded by nonuniform motion blurring. When an inaccurate blur kernel is used as the input, significant distortions will appear in the image recovered by existing methods. In this paper, we present a novel convex minimization model that explicitly takes account of error in the blur kernel. The resulting minimization problem can be efficiently solved by the so-called accelerated proximal gradient method. In addition, a new boundary extension scheme is incorporated in the proposed model to further improve the results. The experiments on both synthesized and real images showed the efficiency and robustness of our algorithm to both the image noise and the model error in the blur kernel. [ABSTRACT FROM AUTHOR]

This paper proposes a new approach to the image blind super-resolution (BSR) problem in the case of affine interframe motion. Although the tasks of image registration, blur identification, and high-resolution (HR) image reconstruction are coupled in the imaging process, when dealing with nonisometric interframe motion or without the exact knowledge of the blurring process, classic SR techniques generally have to tackle them (maybe in some combinations) separately. The main difficulty is that state-of-the-art deconvolution methods cannot be straightforwardly generalized to cope with the space-variant motion. We prove that the operators of affine warping and blur commute with some additional transforms and derive an equivalent form of the BSR observation model. Using this equivalent form, we develop an iterative algorithm to jointly estimate the triple-coupled variables, i.e., the motion parameters, blur kernels, and HR image. Experiments on synthetic and real-life images illustrate the performance of the proposed technique in modeling the space-variant degradation process and restoring local textures. [ABSTRACT FROM AUTHOR]

This paper introduces the concept of digital planar surfaces and corresponding Morse operators. These operators offer a novel and powerful method for construction and de-construction of such surfaces in a way that global topological control of the resulting object is always maintained. In that respect, this paper offers a complete pixel characterization tool. Image handling is a natural application for such approach. We present a novel fast algorithm for image segmentation using Morse operators for digital planar surfaces. It classifies as a region growing technique with added topological control and is extremely useful for applications that need proper object description. Results from real data are stimulating, and show that the segmentation algorithm compares very well with other methods. The topological approach also forms a base for future expansion to applications such as volume segmentation. [ABSTRACT FROM AUTHOR]

Augmented Lagrangian variational formulations and alternating optimization have been adopted to solve distributed parameter estimation problems. The alternating direction method of multipliers (ADMM) is one of such formulations/optimization methods. Very recently, the number of applications of the ADMM, or variants of it, to solve inverse problems in image and signal processing has increased at an exponential rate. The reason for this interest is that ADMM decomposes a difficult optimization problem into a sequence of much simpler problems. In this paper, we use the ADMM to reconstruct piecewise-smooth distributed parameters of elliptical partial differential equations from noisy and linear (blurred) observations of the underlying field. The distributed parameters are estimated by solving an inverse problem with total variation (TV) regularization. The proposed instance of the ADMM solves, in each iteration, an \ell2 and a decoupled \ell2 - \ell1 optimization problems. An operator splitting is used to simplify the treatment of the TV regularizer, avoiding its smooth approximation and yielding a simple yet effective ADMM reconstruction method compared with previously proposed approaches. The competitiveness of the proposed method, with respect to the state-of-the-art, is illustrated in simulated 1-D and 2-D elliptical equation problems, which are representative of many real applications. [ABSTRACT FROM AUTHOR]

The main aim of this paper is to develop an algorithm for blending of multiple images in the image-stitching process. Our idea is to propose a variational method containing an energy functional to determine both a stitched image and weighting mask functions of multiple input images for image blending. The existence of the solution of the proposed energy functional is shown. We also present an alternative minimizing algorithm to solve the proposed model numerically and show the convergence of this algorithm. Experimental results show that the proposed model works effectively and efficiently and that the proposed method is competitive with the tested existing methods under noisy conditions. [ABSTRACT FROM AUTHOR]

A family of the block matching 3-D (BM3D) algorithms for various imaging problems has been recently proposed within the framework of nonlocal patchwise image modeling refid="ref1"/, refid="ref2"/. In this paper, we construct analysis and synthesis frames, formalizing BM3D image modeling, and use these frames to develop novel iterative deblurring algorithms. We consider two different formulations of the deblurring problem, i.e., one given by the minimization of the single-objective function and another based on the generalized Nash equilibrium (GNE) balance of two objective functions. The latter results in the algorithm where deblurring and denoising operations are decoupled. The convergence of the developed algorithms is proved. Simulation experiments show that the decoupled algorithm derived from the GNE formulation demonstrates the best numerical and visual results and shows superiority with respect to the state of the art in the field, confirming a valuable potential of BM3D-frames as an advanced image modeling tool. [ABSTRACT FROM AUTHOR]

This paper studies a problem of image restoration that observed images are contaminated by Gaussian and impulse noise. Existing methods for this problem in the literature are based on minimizing an objective functional having the \ell^1 fidelity term and the Mumford–Shah regularizer. We present an algorithm on this problem by minimizing a new objective functional. The proposed functional has a content-dependent fidelity term which assimilates the strength of fidelity terms measured by the \ell^1 and \ell^2 norms. The regularizer in the functional is formed by the \ell^1 norm of tight framelet coefficients of the underlying image. The selected tight framelet filters are able to extract geometric features of images. We then propose an iterative framelet-based approximation/sparsity deblurring algorithm (IFASDA) for the proposed functional. Parameters in IFASDA are adaptively varying at each iteration and are determined automatically. In this sense, IFASDA is a parameter-free algorithm. This advantage makes the algorithm more attractive and practical. The effectiveness of IFASDA is experimentally illustrated on problems of image deblurring with Gaussian and impulse noise. Improvements in both PSNR and visual quality of IFASDA over a typical existing method are demonstrated. In addition, Fast_IFASDA, an accelerated algorithm of IFASDA, is also developed. [ABSTRACT FROM AUTHOR]

This paper presents a multiphase fuzzy region competition model that takes into account spatial and frequency information for image segmentation. In the proposed energy functional, each region is represented by a fuzzy membership function and a data fidelity term that measures the conformity of spatial and frequency data within each region to (generalized) Gaussian densities whose parameters are determined jointly with the segmentation process. Compared with the classical region competition model, our approach gives soft segmentation results via the fuzzy membership functions, and moreover, the use of frequency data provides additional region information that can improve the overall segmentation result. To efficiently solve the minimization of the energy functional, we adopt an alternate minimization procedure and make use of Chambolle's fast duality projection algorithm. We apply the proposed method to synthetic and natural textures as well as real-world natural images. Experimental results show that our proposed method has very promising segmentation performance compared with the current state-of-the-art approaches. [ABSTRACT FROM AUTHOR]

We address single image super-resolution using a statistical prediction model based on sparse representations of low- and high-resolution image patches. The suggested model allows us to avoid any invariance assumption, which is a common practice in sparsity-based approaches treating this task. Prediction of high resolution patches is obtained via MMSE estimation and the resulting scheme has the useful interpretation of a feedforward neural network. To further enhance performance, we suggest data clustering and cascading several levels of the basic algorithm. We suggest a training scheme for the resulting network and demonstrate the capabilities of our algorithm, showing its advantages over existing methods based on a low- and high-resolution dictionary pair, in terms of computational complexity, numerical criteria, and visual appearance. The suggested approach offers a desirable compromise between low computational complexity and reconstruction quality, when comparing it with state-of-the-art methods for single image super-resolution. [ABSTRACT FROM PUBLISHER]

Focuses on a study which addressed the problem of restoring an image from its noisy convolutions with two or more unknown finite impulse response (FIR) filters. Types of algorithms; Effect of the blind deconvolution problem; Reason nonblind convolution is a problem.

When dealing with motion blur, there is an inevitable tradeoff between the amount of blur and the amount of noise in the acquired images. The effectiveness of any restoration algorithm typically depends on these amounts, and it is difficult to find their best balance in order to ease the restoration task. To face this problem, we provide a methodology for deriving a statistical model of the restoration performance of a given deblurring algorithm in case of arbitrary motion. Each restoration-error model allows us to investigate how the restoration performance of the corresponding algorithm varies as the blur due to motion develops. Our modeling treats the point-spread-function trajectories as random processes and, following a Monte Carlo approach, expresses the restoration performance as the expectation of the restoration error conditioned on some motion-randomness descriptors and on the exposure time. This allows us to coherently encompass various imaging scenarios, including camera shake and uniform (rectilinear) motion, and, for each of these, identify the specific exposure time that maximizes the image quality after deblurring. [ABSTRACT FROM AUTHOR]

Examines a study which presented a multiplicative algorithm for image reconstruction. Aims; Application to infrared astronomical satellite (IRAS); Difficulties in ringing artifact.