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The widespread use of artificial insemination in the pig industry has provided breeders with access to genetic material from superior boars from around the world. Selection of parent stock is based on estimated breeding values (EBVs), which are regularly computed in all countries performing genetic evaluations. The purpose of this study was to evaluate the value of foreign sires in the South African (SA) pig industry by comparing the on-farm performances of progeny for average daily gain (ADG), feed conversion ratio (FCR) and back fat thickness (BF) from progeny sired by USA and Canadian born sires to the performance of progeny from local sires and to progeny with paternal USA grandsires (F1-US sires). The breeds analysed comprised of Duroc, Landrace and Large White pigs. Males outperformed females for all traits measured (P <0.0001). Large differences (P <0.0001) were observed between on-farm performances which had similar climatic regions for all traits except Duroc BF (P <0.05). Farm differences were consequently attributed to management rather than environmental influences. The effect of country was significant (P <0.05) in all the models tested with the USA-sired progeny having the best overall performance. However, as measured by a stepwise R2, country remained the smallest contributor to variation across all models (except BF in Landrace), with farm, sex and year-season contributing larger portions to the variation observed in the onfarm performances of progeny. These results indicate that most of the variation observed could be attributed to differences in farm as explained by management, rather than superiority of the imported boar semen. [ABSTRACT FROM AUTHOR]

Presents information on a study which discussed genetic markers and their application in livestock breeding in South Africa. Description of the restricted fragment length polymorphism technique; Components of microsatellites; Genome mapping efforts.

This study presents the first results on the evaluation and selection of polymorphic microsatellite markers for the genetic characterization of native chicken populations in southern Africa. Blood samples for DNA extraction were obtained from five chicken lines from South Africa (Koekoek, New Hampshire, Naked-Neck, Lebowa-Venda and Ovambo), and from one population each from Mozambique and Botswana. Twenty-three markers were found to be polymorphic in most of the lines studied. The meat number of alleles observed for all markers was 6.1, and varied from 2.3 (Koekoek) to 4.3 (Mozambique population). The lowest heterozygosity was found in the Koekoek line (31.4%), and the highest in the populations from Mozambique (60.7%) and Botswana (61.2%). It was concluded that this particular set of microsatellite markers can be of use a measure of genetic variability in native chicken lines in southern Africa, and should be evaluated as diagnostic tools for breed identification. [ABSTRACT FROM AUTHOR]

Ascaridia galli is one of the most common nematode affecting chickens. This study characterized A . galli parasites collected from South African village chickens of Limpopo ( n = 18) and KwaZulu-Natal ( n = 22) provinces using the 510 bp sequences of cytochrome C oxidase subunit 1 gene of the mitochondrial DNA. Fourteen and 12 polymorphic sites were observed for Limpopo and KwaZulu-Natal sequences, respectively. Six haplotypes were observed in total. Haplotype diversity was high and ranged from 0.749 for Limpopo province to 0.758 for KwaZulu-Natal province isolates. There was no genetic differentiation between A . galli from Limpopo and KwaZulu-Natal provinces. The six South African haplotypes were unique compared to those published in the GeneBank sampled from Hy-line chickens raised under organic farming in Denmark. The utility of cytochrome C oxidase subunit 1 gene as a potential genetic marker for studying A . galli in village chicken populations is presented. [ABSTRACT FROM AUTHOR]

Adaptation of animals to different environments is typically associated with structural and functional genomic variations. High throughput SNP genotyping and next-generation sequencing (NGS) have made it possible to study positive selection footprints and adaptation traits. Nguni is a small frame-size breed, mostly horned, and well known for being adapted to diverse South African environmental conditions. This study used previously identified selective sweeps to perform functional analysis of genes related to phenotypic characteristics in Nguni. Two hundred and sixty-four candidate selective sweeps were used for gene-set enrichment analysis in molecular functional categories (KEGG pathways) using the database for annotation, visualization, and integrated discovery (DAVID). In total, 107 genes were identified across all the chromosomes with 74 genes associated with eight phenotype queries, including fat content, milk production, walking ability, heat tolerance, meat production, reproduction, and bone and muscle development. Gene CRHR2 was associated with meat quality (juiciness and flavour). The IRAK3 gene was associated with decreased body size, feed intake and fatness in cattle, and CARD15 with disease resistance. Gene annotation using phenotype queries identified four genes (SPI, YWHAZ, RGS4, and RGS5) that were associated with myometrial relaxation in cattle. Genes such as NOD2 and IL21R were associated with inflammatory bowel diseases in cattle, whereas CPLS gene was associated with fat content. These genes are important to the phenotypic and adaptive characteristics present in South African Nguni cattle and hold potential for selection for traits of economic importance. [ABSTRACT FROM AUTHOR]