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Leaves and aerial parts of Malva neglecta Wallr. have been traditionally used in Anatolia for the treatment of pain, inflammation, hemorrhoids, renal stones, constipation, and infertility. This study investigated the effects of M. neglecta leaves in a rat endometriosis model. The dried plant material was extracted with n-hexane, ethyl acetate, and methanol, successively. Experimental endometriosis was surgically induced in six-week-old female, non-pregnant, Wistar albino rats by autotransplant of endometrial tissue to the abdominal wall. After twenty-eight days, rats were evaluated for a second laparotomy. Endometrial foci areas were assessed, and intraabdominal adhesions were scored. Rats were divided into five groups as control, n-hexane, ethyl acetate, methanol, and aqueous extracts, as well as reference. At the end of the treatment, all rats were sacrificed and endometriotic foci areas and intraabdominal adhesions were re-evaluated and compared with the previous findings. Moreover, peritoneal fluid was collected to detect tumor necrosis factor- α (TNF-α), vascular endothelial growth factor (VEGF), and interleukin-6 (IL-6) levels, and cDNA synthesis, and a quantitative real-time polymerase chain reaction (PCR) test was done. The phytochemical content of the most active extract was determined using High-Performance Liquid Chromatography (HPLC). Both endometrial volume and adhesion score decreased significantly in the group treated with methanol extract. In addition, significant decreases were observed in TNF-α, VEGF, and IL-6 levels in animals administered methanol extract. HPLC results showed that the activity caused by the methanol extract of M. neglecta was due to the polyphenols. Taken together, these novel findings indicate that M. neglecta may be a promising alternative for the treatment of endometriosis. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the possibility of production of a functional half-fat synbiotic stirred yogurt using Panirak (Malva neglecta) and lactulose (as a prebiotic compound) with an acceptable physicochemical and sensory properties. To produce half-fat synbiotic stirred yogurt samples, Lactobacillus fermentum SL163-4 from a fermented food (Ashkardeh) was isolated and used as probiotic bacteria. Panirak at four levels (0, 5, 10, 15%) and lactulose at three levels (0, 1 and 2%) were used as prebiotic substances. Physicochemical and sensory properties of the yogurt samples were investigated during 21 days of refrigerated storage. Based on the results obtained from the data analysis, it was found that as the level of Panirak enhanced, pH increased and acidity decreased (p<0.05) while by increasing the amount of lactulose and with passing the storage time, pH decreased and acidity increased (p<0.05). Also, by increasing the amount of Panirak and lactulose and with passing the storage time, the extent of syneresis of the yogurt samples decreased (p<0.05). Based on the sensory results, it was found that by addition of Panirak up to 10% level, taste and acceptability of yogurt samples improved (p<0.05), but lactulose caused a significant reduction in these sensory attributes (p<0.05). Furthermore, there were no differences between yogurt samples containing 10 and 15% Panirak and samples having 0 and 1% lactulose. Therefore, according to the results obtained from this study, the use of 15% Panirak and 1% lactulose to produce a functional stirred yoghurt with an acceptable quality is recommended. [ABSTRACT FROM AUTHOR]

The purpose of the present work was to determine the phenolic and the fatty acids profiles by LC-MS/MS and GC/MS with their antioxidant, anticholinesterase and antimicrobial activities, and aflatoxin contents of Malva neglecta and Malvella sherardiana. The phenolic contents of M. neglecta and M. sherardiana were determined by LC-MS/MS. Malic and 4-OH benzoic acids were found to be the most abundant compounds in M. neglecta and M. sherardiana, respectively. On the other hand, essential oil and fatty acid compositions were determined by GC/MS analysis. The methanol extracts of the plants showed the highest effect in all antioxidant assays in this study. The methanol extract of M. neglecta showed the highest activity among the petroleum ether, acetone, methanol and water extracts against acetyl- and butyryl-cholinesterase enzymes (53.68% and 63.95% inhibition ratio, respectively). The acetone extracts of M. neglacta and M. sherardiana exhibited moderate activity against C. albicans with 18 and 17 mm inhibion zone diameter, respectively. The results of the present study is also showed that the M. neglecta and M. sherardiana can also be used as a food source due to its high phenolic acid content and good antioxidant property. Also, the samples were aflatoxin free. [ABSTRACT FROM AUTHOR]

This study was carried out to determine the some nutritional and seed properties of three wild edible plants, namely, Malva neglecta (common mallow), Polygonum cognatum (knotweed) and Trachystemon orientalis (Abraham-Isaac-Jacob), consumed as vegetable in the Middle Black Sea Region of Turkey. In this study, the plants were collected from five different localities in four different provinces (Amasya, Ordu, Samsun and Tokat) of the region. As a result, the nutritional and seed properties of wild edible plants varied considerably depending on the species and locality. Nutritional analysis showed that the wild edible plants contained important levels of protein (15.71 to 19.96%), potassium (1219.19–1867.47 mg/100 g), phosphorus (56.89–195.86 mg/100 g), calcium (282.96–688.32 mg/100 g) and magnesium (112.54–165.79 mg/100 g). The length, width, thickness, geometric mean diameter, sphericity, surface area, volume, 1000 seed weight and bulk density of wild edible plant seeds varied from 1.71 to 3.11 mm, 1.37 to 1.83 mm, 1.00 to 1.61 mm, 1.41 to 2.06 mm, 0.60 to 0.85, 6.27 to 13.29 mm 2 , 1.20 to 3.17 mm 3 , 1.40 to 3.41 g and 532.3 to 680.9 kg/m 3 , respectively. The germination rate and mean germination time of seeds changed from 36.33 to 64.67% and 7.00 to 12.67 day, respectively. The results clearly revealed that these wild plants had important nutritional properties. Thus, these wild plant species could serve as good and cheap food sources in human diet. Additionally, the findings of this study may provide useful information on nutritional composition and seed properties of these wild plants for researchers. [ABSTRACT FROM AUTHOR]

This study addresses the effects of gamma irradiation (1, 5 and 8 kGy) on color, organic acids, total phenolics, total flavonoids, and antioxidant activity of dwarf mallow (Malva neglectaWallr.). Organic acids were analyzed by ultra fast liquid chromatography (UFLC) coupled to a photodiode array (PDA) detector. Total phenolics and flavonoids were measured by the Folin-Ciocalteu and aluminium chloride colorimetric methods, respectively. The antioxidant activity was evaluated based on the DPPH• scavenging activity, reducing power, β-carotene bleaching inhibition and thiobarbituric acid reactive substances (TBARS) formation inhibition. Analyses were performed in the non-irradiated and irradiated plant material, as well as in decoctions obtained from the same samples. The total amounts of organic acids and phenolics recorded in decocted extracts were always higher than those found in the plant material or hydromethanolic extracts, respectively. The DPPH• scavenging activity and reducing power were also higher in decocted extracts. The assayed irradiation doses affected differently the organic acids profile. The levels of total phenolics and flavonoids were lower in the hydromethanolic extracts prepared from samples irradiated at 1 kGy (dose that induced color changes) and in decocted extracts prepared from those irradiated at 8 kGy. The last samples also showed a lower antioxidant activity. In turn, irradiation at 5 kGy favored the amounts of total phenolics and flavonoids. Overall, this study contributes to the understanding of the effects of irradiation in indicators of dwarf mallow quality, and highlighted the decoctions for its antioxidant properties. [ABSTRACT FROM AUTHOR]

Abstract: Several ethnomedicinal plant remedies used in Pinarbasi, Kayseri, in particular those which are used for the treatment of several peptic ulcer symptoms such as stomach ache, heartburn, etc. were selected for evaluation of their anti-ulcerogenic potential. In order to confirm the claimed activities, decoctions were prepared from aerial parts of Malva neglecta Wallr. (Malvaceae), leaves of Potentilla reptans L. (Rosaceae), fruits of Rumex patientia L. (Polygonaceae), aerial parts of Sanguisorba minor Scop. ssp. muricata (Spach) Briq. (Rosaceae), aerial parts of Sideritis caesarea Duman, Aytac&Baser (Lamiaceae), and flowers of Verbascum cheiranthifolium Boiss var. cheiranthifolium (Scrophulariaceae) according to their described folkloric applications. Pharmacological experiments clearly demonstrated that the extracts of all plants given orally showed significant gastric protection against the ethanol-induced gastric ulcer model in rats. Furthermore, healing effects were also confirmed through histopathological examination. [Copyright &y& Elsevier]

The potential involvement of the xanthophyll cycle in photoprotection of overwintering evergreen plants was investigated. Leaves from five evergreen species, Pseudotsuga menziesii, Pinus ponderosa, Euonymus kiautschovicus, Mahonia repens and Malva neglecta, were collected from the field predawn during winter and transferred to the laboratory where chlorophyll fluorescence emission as well as the chlorophyll and carotenoid composition were ascertained periodically for 4.5 days. Leaves and needles from all species were found to have retained large amounts of the xanthophyll cycle pigments zeaxanthin and antheraxanthin, and they exhibited sustained low values of the intrinsic efficiency of photosystem II (PSII; measured as the ratio of variable to maximal fluorescence, Fv/Fm) upon collection. The increase in PSII efficiency was biphasic, with a rapid phase (requiring several hours) and a slow phase (requiring several days). Changes in the conversion state of the xanthophyll cycle were found to correlate with increases in PSII efficiency in both phases, with the latter phase involving large increases in both Fm (maximal fluorescence) and Fo (minimal fluorescence) throughout the period of recovery. The relationship between Fm quenching (expressed as nonphotochemical or Stern-Vohner quenching [NPQ] of Fm, i.e. Fm/F′m-1) and Fo quenching (Fo/F′o-1) was linear, as expected for changes in xanthophyll cycle-dependent energy dissipation in the antenna complexes. Furthermore, the relationship between Fv/Fm and NPQ during recovery followed the theoretical relationship predicted for changes in the rate constant for energy dissipation in the antenna complexes. This fit between the theoretical relationship and the actual data indicates that all changes in NPQ or Fv/Fm can be accounted for by changes in this rate constant. The results suggest a role for the photoprotective xanthophyll cycle-dependent dissipation process in the lowered efficiency of PSII observed in cold-stressed evergreen plants in the field. [ABSTRACT FROM AUTHOR]

The use of herbal medicines is increasing in developed countries as alternative and/or supportive therapy to conventional health care medicines. Malva neglecta Wallr. (Family: Malvaceae) has been reported as wound healing remedy in traditional medicines, however no experimental data is available on its wound healing potentials. The aim of this study was to explore phytochemistry and validate wound healing potentials of the plant using animal models. M. neglecta crude methanolic extract (Mn.Cme) was chemically characterized using HPLC-DAD and GCMS analysis. Acute dermal toxicity was determined in albino rats following Organization of Economic Co-operation and Development (OECD) 402 established standards. Wound healing potentials were evaluated in rats using excision wound model. Wounds (177 mm2) were made by an excision on the skin of rats which were placed individually in cages. Mn.Cme was formulated in ointment form and was applied topically onto the wound area once daily for 14 days. The wound area was measured with translucent paper and thereafter estimated on a 1 mm2 graph sheet every 3rd day until epithelialization and complete wound closure was recorded. Wound contraction was calculated as a percentage of the original wound size. Antioxidant potentials were also evaluated via FRAP, DPPH and H 2 O 2 free radicals scavenging assays. HPLC-DAD analysis revealed 25 phenolic compounds with higher amounts of hydrotyrosol (109.3 mg/g), coumaroylhexoside (97.4 mg/g), kaempferol-3-(p-coumaroyldiglucoside)-7-glucoside (37.2 mg/g), quercetin-3- O -rutinoside (31.5 mg/g) and epicatechin-3- O -(4- O -methyl)-gallate (31.3 mg/g). In GC-MS analysis, oleic acid (19.67%), taurine (17.60%), ethylene dimercaptan (14.67%), isoeugenol (14.61%), patchoulane (10.36%), methyl 12-methyltetradecanoate (8.47%) and isopropyl myristate (7.02%) were highly abundant compounds. No sign of toxicity was observed in the acute dermal toxicity test. Our test sample (Mn.Cme) exhibited considerable wound healing tendency at all doses (1 g, 1.5 g, 2 g per 10 g of ointment base) with reduced epithelialization period in a dose–related manner. Absolute healing was observed after application of 2 g of Mn.Cme ointment. Further, Mn.Cme exhibited considerable anti-radical potential in all assays. It may be concluded that M. neglecta possess very potent secondary metabolites which are previously reported for wound healing potentials. The plant has considerable antioxidant and wound healing properties and thus warrant further studies to uncover the molecular mechanism its wound healing potentials. Image 1 [ABSTRACT FROM AUTHOR]