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Summary: Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB‐BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB‐BD strain was sequenced and analysed. Genomic analysis showed that the HB‐BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains. [ABSTRACT FROM AUTHOR]

This work aimed to develop an indirect enzyme-linked immunosorbent assay (i- ELISA), based on the structural protein VP2 of goose parvovirus ( GPV), for the detection of antibodies to GPV in blood serum. In this study, the capsid protein VP2 of GPV was expressed in Escherichia coli and purified by nickel chromatography. The purified protein was capable of reacting with GPV antibodies, as analysed by Western blot. An i- ELISA based on the protein VP2 was developed. For evaluation of the newly developed ELISA, 192 field sera collected from six goose herds were tested in parallel by the serum neutralization ( SN) test. The result showed that 138 samples were positive and 49 negative in both cases. The agreement of the two tests was 97·3%. This i- ELISA has high sensitivity and good specificity and is an alternative serologic test for the detection of the antibodies to GPV in blood serum. Significance and Impact of the Study In this study, we expressed and purified the capsid protein VP2 of GPV and developed a VP2-based i- ELISA for the detection of GPV antibodies. This i- ELISA can be used for evaluating the immune effects of GPV vaccine and for diagnosing of GPV infection. [ABSTRACT FROM AUTHOR]