1. Antigenicity evaluation of synthetic α-(1,3)-linked D, D-heptoglycan of <italic>Helicobacter pylori</italic> serotype O6 lipopolysaccharide.
- Author
-
Xiao, Lei, Zou, Xiaopeng, Tian, Guangzong, Qin, Chunjun, Zhao, Hui, Fu, Junjie, Hu, Jing, and Yin, Jian
- Abstract
Abstract
Helicobacter pylori , a gram‐negative bacterium, is known to be associated with various gastric pathologies including chronic gastritis, peptic ulcers and gastric carcinoma. Despite the emerging issue of bacterial resistance to antibiotic-based combination therapy, there is currently no vaccine available forH. pylori infection in the market. Here, we report the synthesis of α-(1,3)-D,D-heptoglycan with different chain length from the lipopolysaccharide ofH. pylori serogroup O6. The [n + 1] iterative glycosylation strategy was used for heptoglycan chain elongation. The trifluoroacetimidate heptoside donor exhibited much higher efficiency than thioglycoside donor during glycosylation. An antigenicity evaluation using glycan microarrays indicated that α-(1,3)-D,D-hepto-disaccharide, pentasaccharide and hexasaccharide showed stronger binding affinity to IgG antibodies ofH. pylori O6 LPS immunized rabbit serum or serumof H. pylori infected patients. These findings provide significant structure − activity relationship information for developing carbohydrate-based vaccines againstH. pylori containing α-(1,3)-D,D-heptoglycan. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF