41 results on '"Zhu, Xuesong"'
Search Results
2. A Real‐world Study of Denosumab For Reducing Refracture Risk after Percutaneous Vertebral Augmentation.
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Zou, Jun, Zhang, Yijian, Niu, Junjie, Song, Dawei, Huang, Zhenna, Li, Zongjie, Liu, Tao, Meng, Bin, Shi, Qin, Zhu, Xuesong, and Yang, Huilin
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VERTEBRAL fractures , *BONE health , *BONE fractures , *DATABASES , *DENOSUMAB , *VERTEBRAE injuries - Abstract
Objective: To investigate the use of anti‐osteoporotic agents and refracture incidence in patients with osteoporotic vertebral compression fracture (OVCF) following percutaneous vertebral augmentation (PVA) and to evaluate the real‐world treatment of patients using denosumab following PVA. This study aims to provide spine surgeons with empirical insights derived from real‐world scenarios to enhance the management of bone health in OVCF patients. Methods: This retrospective cohort study was based on data from the MarketScan and Optum databases from the USA. Female patients aged 55–90 years who underwent PVA for OVCF between January 2013 and March 2020 were included and followed up from the day after surgery. Patients who received at least one dose of denosumab were included in the denosumab cohort and were further divided into the on‐treatment and off‐treatment groups according to whether they received a second dose of denosumab, with follow‐up beginning on the index day (225 days after the first denosumab dose). In this study, the off‐treatment group was considered as the control group. Refracture incidence after PVA, the proportion of patients using anti‐osteoporotic agents in the total study population, and refracture incidence after the index day in the denosumab cohort were analyzed. Results: A total of 13,451 and 21,420 patients from the MarketScan and Optum databases, respectively, were included. In the denosumab cohort, the cumulative incidence of clinical osteoporotic fractures within 3 years after the index day was significantly lower in the on‐treatment group than in the off‐treatment group (MarketScan database: 23.0% vs 39.0%, p = 0.002; Optum database: 28.2% vs 40.0%, p = 0.023). The cumulative incidence of clinical vertebral fractures was also lower in the on‐treatment group than in the off‐treatment group, with a significant difference in the MarketScan database (14.4% vs 25.5%, p = 0.002) and a numerical difference was found in the Optum database (20.2% vs 27.5%, p = 0.084).The proportion of patients using anti‐osteoporotic agents was low at 6 months postoperatively, with only approximately 7% using denosumab and 13%–15% taking oral bisphosphonates. Conclusion: Postmenopausal women have a high refracture rate and a low proportion of anti‐osteoporotic drug use after PVA. Continued denosumab treatment after PVA is associated with a lower risk of osteoporotic and clinical vertebral fractures. Therefore, denosumab may be a treatment option for patients with osteoporosis after PVA. [ABSTRACT FROM AUTHOR]
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- 2024
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3. Kyphoplasty is associated with reduced mortality risk for osteoporotic vertebral compression fractures: a systematic review and meta-analysis.
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Zhang, Yijian, Ge, Jun, Liu, Hao, Niu, Junjie, Wang, Shenghao, Shen, Hao, Li, Hanwen, Qian, Chen, Song, Zhuorun, Zhu, Pengfei, Zhu, Xuesong, Zou, Jun, and Yang, Huilin
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VERTEBRAL fractures , *KYPHOPLASTY , *OSTEOPOROSIS , *SCIENCE databases , *WEB databases - Abstract
Background: Vertebral augmentation, such as vertebroplasty (VP) or kyphoplasty (KP), has been utilized for decades to treat OVCFs; however, the precise impact of this procedure on reducing mortality risk remains a topic of controversy. This study aimed to explore the potential protective effects of vertebral augmentation on mortality in patients with osteoporotic vertebral compression fractures (OVCFs) using a large-scale meta-analysis. Materials and methods: Cochrane Library, Embase, MEDLINE, PubMed and Web of Science databases were employed for literature exploration until May 2023. The hazard ratios (HRs) and 95% confidence intervals (CIs) were utilized as a summary statistic via random-effect models. Statistical analysis was executed using Review Manager 5.3 software. Results: After rigorous screening, a total of five studies with substantial sample sizes were included in the quantitative meta-analysis. The total number of participants included in the study was an 2,421,178, comprising of 42,934 cases of vertebral augmentation and 1,991,244 instances of non-operative management. The surgical intervention was found to be significantly associated with an 18% reduction in the risk of mortality (HR 0.82; 95% CI 0.78, 0.85). Subgroup analysis revealed a remarkable 71% reduction in mortality risk following surgical intervention during short-term follow-up (HR 0.29; 95% CI 0.26, 0.32). Furthermore, KP exhibited a superior and more credible decrease in the risk of mortality when compared to VP treatment. Conclusions: Based on a comprehensive analysis of large samples, vertebral augmentation has been shown to significantly reduce the mortality risk associated with OVCFs, particularly in the early stages following fractures. Furthermore, it has been demonstrated that KP is more reliable and effective than VP in terms of mitigating mortality risk. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Melatonin rescues the mitochondrial function of bone marrow‐derived mesenchymal stem cells and improves the repair of osteoporotic bone defect in ovariectomized rats.
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Gu, Chao, Zhou, Quan, Hu, Xiayu, Ge, Xiaoyang, Hou, Mingzhuang, Wang, Wenhao, Liu, Hao, Shi, Qin, Xu, Yong, Zhu, Xuesong, Yang, Huilin, Chen, Xi, Liu, Tao, and He, Fan
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MESENCHYMAL stem cells , *OSTEOPOROSIS , *MITOCHONDRIA , *MELATONIN , *METABOLIC regulation - Abstract
Osteoporotic bone defects, a severe complication of osteoporosis, are distinguished by a delayed bone healing process and poor repair quality. While bone marrow‐derived mesenchymal stem cells (BMMSCs) are the primary origin of bone‐forming osteoblasts, their mitochondrial function is impaired, leading to inadequate bone regeneration in osteoporotic patients. Melatonin is well‐known for its antioxidant properties and regulation on bone metabolism. The present study postulated that melatonin has the potential to enhance the repair of osteoporotic bone defects by restoring the mitochondrial function of BMMSCs. In vitro administration of melatonin at varying concentrations (0.01, 1, and 100 μM) demonstrated a significant dose‐dependent improvement in the mitochondrial function of BMMSCs obtained from ovariectomized rats (OVX‐BMMSCs), as indicated by an elevation in mitochondrial membrane potential, adenosine triphosphate synthesis and expression of mitochondrial respiratory chain factors. Melatonin reduced the level of mitochondrial superoxide by activating the silent information regulator type 1 (SIRT1) and its downstream antioxidant enzymes, particularly superoxide dismutase 2 (SOD2). The protective effects of melatonin were found to be nullified upon silencing of Sirt1 or Sod2, underscoring the crucial role of the SIRT1‐SOD2 axis in the melatonin‐induced enhancement of mitochondrial energy metabolism in OVX‐BMMSCs. To achieve a sustained and localized release of melatonin, silk fibroin scaffolds loaded with melatonin (SF@MT) were fabricated. The study involved the surgical creation of bilateral femur defects in OVX rats, followed by the implantation of SF@MT scaffolds. The results indicated that the application of melatonin partially restored the mitochondrial energy metabolism and osteogenic differentiation of OVX‐BMMSCs by reinstating mitochondrial redox homeostasis. These findings suggest that the localized administration of melatonin through bone implants holds potential as a therapeutic approach for addressing osteoporotic bone defects. [ABSTRACT FROM AUTHOR]
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- 2024
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5. The extraordinarily large vortex structure of Typhoon In-fa (2021), observed by spaceborne microwave radiometer and synthetic aperture radar.
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Sun, Ziyao, Bai, Lina, Zhu, Xuesong, Huang, Xin, Jin, Rui, Yu, Hui, and Tang, Jie
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MICROWAVE remote sensing , *MICROWAVE radiometers , *SYNTHETIC aperture radar , *SPACE-based radar , *TYPHOONS , *SYNTHETIC apertures , *AUTOMATIC meteorological stations , *LANDFALL , *TROPICAL cyclones - Abstract
Tropical cyclone (TC) vortex structure can be estimated from wind radii (34, 50, and 64 kt). Accurate wind radii are essential for assessing the impact range of TCs. Combining observations from active and passive microwave remote sensing instruments can provide long-time series data for monitoring changes in TC wind structure. Here, the evolution of the wind radii for Typhoon In-fa (2021), from its genesis to its first landfall, is evaluated using data from spaceborne microwave radiometers and synthetic aperture radars (SARs). Our results show that the retrieved wind radii are relatively close to those from the Joint Typhoon Warning Center (JTWC), the coastal automatic weather stations in China, and the advanced scatterometer. The time series for the wind radii show significant increasing trends during the lifecycle of In-fa and a massive vortex structure before In-fa made landfall. The average wind radii observed before landfall are all likely in the 90th percentile of the 2001–2021 JTWC historical wind radii records, indicating that the vortex structure is extraordinarily large before In-fa made landfall and represents a historical extreme. The extremely large vortex structure may be attributable to the interaction between In-fa and an extremely strong monsoon gyre. [Display omitted] • Wind radii for Typhoon In-fa (2021) are evaluated by multiple spaceborne microwave radiometers and SARs. • The retrieved wind radii are close to those from JTWC, AWSs in China, and ASCAT. • The significant increasing trends of wind radii and a massive vortex structure before landfall are revealed. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Synthesis and biological activities of cyclanone O -(2-(3-aryl-4- amino-4 H -1,2,4-triazol-3-yl)thio)acetyl)oxime derivatives.
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Chen, Meihang, Chen, Lijuan, Zhu, Xuesong, Wang, Xiaobin, Li, Qin, Zhang, Juping, Lu, Daowang, and Xue, Wei
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OXIME derivatives , *CHEMICAL synthesis , *CHEMICAL structure , *MOLECULAR spectroscopy , *ANTIBACTERIAL agents - Abstract
Twelve cyclanoneO-(2-(3-aryl-4-amino-4H-1,2,4-triazol-3-yl)thio)acetyl)oxime derivatives were synthesized and their structures were confirmed by spectroscopy (IR,1H NMR,13C NMR,19F NMR) and elemental analysis. Their antifungal and antibacterial activities were evaluated against six fungi (Gibberella zeae, Fusarium oxysporum, Clematis mandshurica, Phytophthora infestans, Paralepetopsis sasakii, Sclerotinia sclerotiorum) and two bacteria (Xanthomonas oryzaepv.oryzae(Xoo),Xanthomonas citrisubsp.Citri(Xcc)). The results indicated that most of the title compounds exhibited good antibacterial activities. Among them, compounds6d, 6g, 6h, and6jshowed better antibacterial activities againstXooandXccthan that of the commercial agent thiodiazole-copper. [ABSTRACT FROM AUTHOR]
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- 2017
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7. Therapeutic Effects of FK506 on Prevent Intervertebral Disc Degeneration through Suppression of IL-2.
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Zou, Jun, Zhu, Xuesong, and Yang, Huilin
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- 2013
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8. Reprogramming of Mitochondrial Respiratory Chain Complex by Targeting SIRT3‐COX4I2 Axis Attenuates Osteoarthritis Progression.
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Zhang, Yijian, Liu, Yang, Hou, Mingzhuang, Xia, Xiaowei, Liu, Junlin, Xu, Yong, Shi, Qin, Zhang, Zhongmin, Wang, Liang, Shen, Yifan, Yang, Huilin, He, Fan, and Zhu, Xuesong
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MITOCHONDRIA , *CYTOCHROME oxidase , *EXTRACELLULAR matrix , *OSTEOARTHRITIS , *SIRTUINS - Abstract
Mitochondrial homeostasis is of great importance for cartilage integrity and associated with the progression of osteoarthritis (OA); however, the underlying mechanisms are unknown. This study aims to investigate the role of mitochondrial deacetylation reaction and investigate the mechanistic relationship OA development. Silent mating type information regulation 2 homolog 3 (SIRT3) expression has a negative correlation with the severity of OA in both human arthritic cartilage and mice inflammatory chondrocytes. Global SIRT3 deletion accelerates pathological phenotype in post‐traumatic OA mice, as evidenced by cartilage extracellular matrix collapse, osteophyte formation, and synovial macrophage M1 polarization. Mechanistically, SIRT3 prevents OA progression by targeting and deacetylating cytochrome c oxidase subunit 4 isoform 2 (COX4I2) to maintain mitochondrial homeostasis at the post‐translational level. The activation of SIRT3 by honokiol restores cartilage metabolic equilibrium and protects mice from the development of post‐traumatic OA. Collectively, the loss of mitochondrial SIRT3 is essential for the development of OA, whereas SIRT3‐mediated proteins deacetylation of COX4I2 rescues OA‐impaired mitochondrial respiratory chain functions to improve the OA phenotype. Herein, the induction of SIRT3 provides a novel therapeutic candidate for OA treatment. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Balloon kyphoplasty for osteoporotic Kümmell's disease
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Wang, Genlin, Zhu, Xuesong, and Gan, Minfeng
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- 2010
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10. The degradation of bioactive bone cement for vertebroplasty and kyphoplasty in vivo
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Zhu, Xuesong, Chen, Xiaoqing, Chen, Chunmao, Wang, Genlin, Gu, Yong, Geng, Dechun, Zhang, Zhiming, and Yang, Huilin
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- 2010
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11. Recharge of chondrocyte mitochondria by sustained release of melatonin protects cartilage matrix homeostasis in osteoarthritis.
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Zhang, Yijian, Hou, Mingzhuang, Liu, Yang, Liu, Tao, Chen, Xi, Shi, Qin, Geng, Dechun, Yang, Huilin, He, Fan, and Zhu, Xuesong
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CARTILAGE , *DRUG delivery systems , *EXTRACELLULAR matrix , *MITOCHONDRIA , *HOMEOSTASIS - Abstract
Recent evidence indicates that the mitochondrial functions of chondrocytes are impaired in the pathogenesis of osteoarthritis (OA). Melatonin can attenuate cartilage degradation through its antioxidant functions. This study aims to investigate whether melatonin could rescue the impaired mitochondrial functions of OA chondrocytes and protect cartilage metabolism. OA chondrocytes showed a compromised matrix synthesis capacity associated with mitochondrial dysfunction and aberrant oxidative stress. In vitro treatments with melatonin promoted the expression of cartilage extracellular matrix (ECM) components, improved adenosine triphosphate production, and attenuated mitochondrial oxidative stress. Mechanistically, either silencing of SOD2 or inhibition of SIRT1 abolished the protective effects of melatonin on mitochondrial functions and ECM synthesis. To achieve a sustained release effect, a melatonin‐laden drug delivery system (DDS) was developed and intra‐articular injection with DDS successfully improved cartilage matrix degeneration in a posttraumatic rat OA model. These findings demonstrate that melatonin‐mediated recharge of mitochondria to rescue the mitochondrial functions of chondrocytes represents a promising therapeutic strategy to protect cartilage from OA. [ABSTRACT FROM AUTHOR]
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- 2022
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12. Arctiin-reinforced antioxidant microcarrier antagonizes osteoarthritis progression.
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Liu, Yang, Hou, Mingzhuang, Pan, Zejun, Tian, Xin, Zhao, Zhijian, Liu, Tao, Yang, Huilin, Shi, Qin, Chen, Xi, Zhang, Yijian, He, Fan, and Zhu, Xuesong
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ANTIOXIDANTS , *NUCLEAR factor E2 related factor , *PROTEINASES , *GELLAN gum - Abstract
Loss of extracellular matrix (ECM) of cartilage due to oxidative stress injury is one of the main characteristics of osteoarthritis (OA). As a bioactive molecule derived from the traditional Chinese Burdock, arctiin exerts robust antioxidant properties to modulate redox balance. However, the potential therapeutic effects of arctiin on OA and the underlying mechanisms involved are still unknown. Based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) tool, Burdock-extracted small molecule arctiin was identified as a potential anti-arthritic component. In vitro, treatment using arctiin rescued the interleukin (IL)-1β-induced activation of proteinases and promoted the cartilage ECM synthesis in human chondrocytes. In vivo, intraperitoneal injection of arctiin ameliorated cartilage erosion and encountered subchondral bone sclerosis in the post-traumatic OA mice. Transcriptome sequencing uncovered that arctiin-enhanced cartilage matrix deposition was associated with restricted oxidative stress. Mechanistically, inhibition of nuclear factor erythroid 2-related factor 2 (NRF2) abolished arctiin-mediated anti-oxidative and anti-arthritic functions. To further broaden the application prospects, a gellan gum (GG)-based bioactive gel (GG-CD@ARC) encapsulated with arctiin was made to achieve long-term and sustained drug release. Intra-articular injection of GG-CD@ARC counteracted cartilage degeneration in the severe (12 weeks) OA mice model. These findings indicate that arctiin may be a promising anti-arthritic agent. Furthermore, GG-modified bioactive glue loaded with arctiin provides a unique strategy for treating moderate to severe OA. [ABSTRACT FROM AUTHOR]
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- 2022
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13. Release Kinetics and In Vitro Characterization of Sodium Percarbonate and Calcium Peroxide to Oxygenate Bioprinted Tissue Models.
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Ke, Dongxu, Kengla, Carlos, Lee, Sang Jin, Yoo, James J., Zhu, Xuesong, and Murphy, Sean Vincent
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BIOPRINTING , *CALCIUM , *SODIUM , *PEROXIDES , *TISSUE engineering , *RYANODINE receptors - Abstract
Oxygen-generating materials have been used in several tissue engineering applications; however, their application as in situ oxygen supply within bioprinted constructs has not been deeply studied. In this study, two oxygen-generating materials, sodium percarbonate (SPO) and calcium peroxide (CPO), were studied for their oxygen release kinetics under a 0.1% O2 condition. In addition, a novel cell-culture-insert setup was used to evaluate the effects of SPO and CPO on the viability of skeletal muscle cells under the same hypoxic condition. Results showed that SPO had a burst oxygen release, while CPO had a more stable oxygen release than SPO. Both SPO and CPO reduced cell viability when used alone. The addition of catalase in SPO and CPO increased the oxygen release rate, as well as improving the viability of skeletal muscle cells; however, CPO still showed cytotoxicity with catalase. Additionally, the utilization of 1 mg/mL SPO and 20 U catalase in a hydrogel for bioprinting significantly enhanced the cell viability under the hypoxic condition. Moreover, bioprinted muscle constructs could further differentiate into elongated myotubes when transferring back to the normoxic condition. This work provides an excellent in vitro model to test oxygen-generating materials and further discover their applications in bioprinting, where they represent promising avenues to overcome the challenge of oxygen shortage in bioprinted constructs before their complete vascularization. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Balloon kyphoplasty for osteoporotic spinal fractures with middle column compromise.
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Gan, Minfeng, Zou, Jun, Zhu, Xuesong, Wang, Genlin, and Yang, Huilin
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SPINAL cord injuries , *COMPRESSION fractures , *OSTEOPOROSIS , *RETROSPECTIVE studies , *INTERVERTEBRAL disk prostheses , *THERAPEUTICS - Abstract
Background Balloon kyphoplasty (BKP) is an effective method for osteoporotic vertebral compression fractures. However osteoporotic spinal fractures with middle column compromise are mentioned as a relative contraindication to BKP. Thus we investigated the safety and efficacy of BKP in the treatment of osteoporotic spinal fractures with middle column compromise but without neurological deficit. Methods In this retrospective study, 45 patients who suffered osteoporotic fractures with middle column compromise but without neurological deficits were treated by BKP from May 2007 to December 2010. The final follow-ups were finished during the time of July 2011–September 2011. The mean follow-up period was 20.2 months. The height of the compromised vertebral body, the kyphotic angle and spinal canal compromise were measured before surgery, one day after surgery, and at the final follow-up. A visual analogue scale (VAS) and the Oswestry disability index (ODI) were chosen to evaluate pain and functional activity. Results The mean VAS and ODI scores improved significantly from pre- to post-operation ( p < 0.05), and this improvement was sustained at the final follow-up. The mean anterior vertebral body height ratio improved from 57.6% ± 11.8% preoperatively to 86.2% ± 12.2% postoperatively ( p < 0.05), so did the mean middle vertebral body height ratio. The kyphotic angle improved from 16.3° ± 3.7° preoperatively to 9.3° ± 2.6° postoperatively ( p < 0.05). At final follow-up, BKP stabilised vertebral height and prevented further kyphotic deformity. While there were no differences in spinal canal compromise between pre-operation and one day after surgery ( p > 0.05), there was a significant difference from the measurement at the final follow-up ( p < 0.05). Conclusion BKP is a safe and effective method for osteoporotic spinal fractures with middle column compromise but without neurological deficit. Spontaneous remodelling of the spinal canal also occurs after BKP. [ABSTRACT FROM AUTHOR]
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- 2014
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15. Cyclic amplification of remodeling bone regeneration process via cerium-energized spinning hydrogel biomembrane for rescuing osteoporotic bone defects.
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Hou, Mingzhuang, Deng, Yaoge, Lv, Nanning, Wu, Yubin, Zhu, Yanrun, Zhang, Yijian, Liu, Yang, Xia, Xiaowei, Yu, Chenqi, Yu, Jianfeng, He, Fan, Xu, Yong, and Zhu, Xuesong
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BONE regeneration , *BONE remodeling , *HYDROGELS , *OSTEOPOROSIS , *BONE resorption , *EXTRACELLULAR matrix , *TERIPARATIDE - Abstract
• Hydrogel spinning membrane achieved a controllable and responsive release of cerium ions. • Establishing a beneficial self-amplifying cycle between cerium release and accelerating osteogenesis. • Simultaneously exerts the "one stone, three birds" effect of promoting osteogenesis, inhibiting osteoclasts, and accelerating angiogenesis. The heightened activity of osteoclasts and diminished function of osteoblasts observed in individuals with osteoporosis present additional complexities in the management of critical bone defects. Consequently, an improved approach is urgently required to enhance bone regeneration in patients with osteoporosis. Our effective strategy involves the integration of hydrogels with spinning membrane technology, and accurately replicates the composition and structure of the native extracellular matrix found in periosteum tissues. Cerium, as a substitute for conventional protein growth factors, facilitates osteogenic differentiation and angiogenesis in stem cells, concurrently impeding bone resorption. In this study, we successfully synthesized a biomimetic electrospun hydrogel periosteum, grafted cerium ions onto the membrane via non-covalently bound phosphate, and achieved cyclic amplification of bone regeneration. This approach represents a pioneering and efficient utilization of tissue-engineered bionic periosteum to address osteoporotic bone defects. [ABSTRACT FROM AUTHOR]
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- 2024
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16. Rare soil species impact the rhizosphere bacterial communities and cadmium uptake by Lolium multiflorum Lam.
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Gao, Chenxin, Zhu, Yan, Xiao, Xian, Liu, Rui, Zhu, Xuesong, and Zhao, Yuan
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SOIL microbial ecology , *ENDANGERED species , *RHIZOSPHERE , *BACTERIAL communities , *ITALIAN ryegrass , *BIOLOGICAL extinction , *SOIL remediation - Abstract
Purpose: Diverse rare taxa are increasingly recognized as drivers of key functions in ecosystems. However, less attention has been given to their importance in the remediation of metal-contaminated soils by phytoextraction. Methods: Here, we studied the effect of rare soil species loss on Cd uptake by Lolium multiflorum Lam. with a pot experiment using dilution-to-extinction and explored the underlying mechanisms by detecting the rhizosphere microbiome. Results: Loss of rare soil microbes greatly decreased plant Cd uptake by 52.34–73.71%, suggesting the lack of functional redundancy of the microbial community in mediating phytoremediation. By sequencing rhizosphere microbial 16S rRNA gene amplicons, we found that increasing rare species loss reduced the rhizosphere alpha diversity and shifted the community composition. Rhizosphere networks were less complex and stable, reflected by decreasing network edges and a negative/positive correlation ratio (neg/pos) with dilution. Random forest and structural equation modelling suggested the importance of rhizosphere alpha diversity and the network neg/pos index in plant Cd uptake, as rare members or their coexisting species might affect the root uptake of Cd through accidental transport of essential elements. Conclusion: Our results indicated the important role of rare species in the phytoremediation of Cd-contaminated soils, highlighting the necessity of protecting the rare biosphere. [ABSTRACT FROM AUTHOR]
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- 2022
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17. Delivering cells via ECM mimetic hydrogel microspheres for promoting the reconstruction of rat lumbar nucleus pulposus.
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Liu, Hao, Tian, Xin, Jin, Chenyang, Li, Yangfeng, Zhu, Xuesong, Pei, Ming, Liu, Tao, Yang, Huilin, Xu, Yong, and He, Fan
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NUCLEUS pulposus , *MICROSPHERES , *INTERVERTEBRAL disk , *ADENOSINE triphosphate , *REACTIVE oxygen species , *BIOMIMETIC materials - Abstract
• GelMA and fucoidan hydrogel microspheres were developed to resemble native NP matrix. • GelMA@FD microspheres supported NP cell proliferation and promoted ECM synthesis. • GelMA@FD improved mitochondrial energy metabolism by regulating redox homeostasis. • Injection of cell-laden GelMA@FD microspheres reconstructed NP tissues in rat lumbar IVDs. Nucleus pulposus (NP) discectomy is a clinical procedure for the treatment of late-stage intervertebral disc (IVD) degeneration; however, it is associated with the potential for serious complications. The reconstruction of the NP tissue presents challenges due to its avascular structure and prolonged compression within the IVD. In this study, we have developed a novel biomimetic hydrogel microsphere composed of gelatin methacryloyl (GelMA) and fucoidan, a polysaccharide derived from marine brown seaweed, to mimic the extracellular matrix (ECM) of the native NP. The GelMA and fucoidan (GelMA@FD) hydrogel microspheres demonstrate favorable cytocompatibility and significantly enhance the synthesis of matrix components in nucleus pulposus cells (NPCs), including aggrecan and type II collagen. Transcriptome sequencing analysis demonstrates a correlation between the augmentation of matrix synthesis and the enrichment of genes and pathways related to mitochondrial function. Subsequent molecular investigations provide evidence that NPCs cultured on GelMA@FD exhibit enhanced mitochondrial functions, including elevated adenosine triphosphate production and respiratory chain factor expression, alongside a reduction in mitochondrial reactive oxygen species. When implantated into rat lumbar IVDs that have undergone discectomy, the incorporation of NPCs within GelMA@FD hydrogel microspheres facilitates the regeneration of the NP and reinstates its structural and biomechanical properties. This is substantiated by the observed augmentation in IVD disc height and water content within the NP compartment. Collectively, these results demonstrate the potential of employing cell-laden GelMA@FD hydrogel microspheres as a promising strategy for NP regeneration in degenerated IVDs. [ABSTRACT FROM AUTHOR]
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- 2024
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18. Does zero-profile anchored cage accompanied by a higher postoperative subsidence compared with cage-plate construct? A meta-analysis.
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Lu, Yingjie, Fang, Yuepeng, Shen, Xu, Lu, Dongdong, Zhou, Liyu, Gan, Minfeng, and Zhu, Xuesong
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CERVICAL vertebrae , *DISCECTOMY , *MEDICAL information storage & retrieval systems , *MEDLINE , *META-analysis , *ONLINE information services , *POSTOPERATIVE period , *SPINAL fusion , *SYSTEMATIC reviews , *DATA analysis software , *DESCRIPTIVE statistics - Abstract
Background: The zero-profile anchored cage (ZP) has been widely used for its lower occurrence of dysphagia. However, it is still controversial whether it has the same stability as the cage-plate construct (CP) and increases the incidence of postoperative subsidence. We compared the rate of subsidence after anterior cervical discectomy and fusion (ACDF) with ZP and CP to determine whether the zero-profile device had a higher subsidence rate. Methods: We performed a meta-analysis of studies that compared the subsidence rates of ZP and CP. An extensive and systematic search covered the PubMed and Embase databases according to the PRISMA guidelines and identified ten articles that satisfied our inclusion criteria. Relevant clinical and radiological data were extracted and analyzed by the RevMan 5.3 software. Results: Ten trials involving 626 patients were included in this meta-analysis. The incidence of postoperative subsidence in the ZP group was significantly higher than that in the CP group [15.1% (89/588) versus 8.8% (51/581), OR = 1.97 (1.34, 2.89), P = 0.0005]. In the subgroup analysis, we found that the definition of subsidence did not affect the higher subsidence rate in the ZP group. Considering the quantity of operative segments, there was no significant difference in the incidence of subsidence between the two groups after single-level fusion (OR 1.43, 95% CI 0.61–3.37, P = 0.41). However, the subsidence rate of the ZP group was significantly higher than that of the CP group (OR 2.61, 95% CI 1.55–4.40, P = 0.0003) after multilevel (≥ 2-level) procedures. There were no significant differences in intraoperative blood loss, JOA score, NDI score, fusion rate, or cervical alignment in the final follow-up between the two groups. In addition, the CP group had a longer operation time and a higher incidence of dysphagia than the ZP group at each follow-up time. Conclusion: Based on the limited evidence, we suggest that ZP has a higher risk of postoperative subsidence than CP, although with elevated swallowing discomfort. A high-quality, multicenter randomized controlled trial is required to validate our results in the future. [ABSTRACT FROM AUTHOR]
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- 2020
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19. Comparison of cervical disc arthroplasty and anterior cervical discectomy and fusion for the treatment of cervical disc degenerative diseases on the basis of more than 60 months of follow-up: a systematic review and meta-analysis.
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Zhang, Yijian, Lv, Nanning, He, Fan, Pi, Bin, Liu, Hao, Chen, Angela Carley, Yang, Huilin, Liu, Mingming, and Zhu, Xuesong
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DISCECTOMY , *DEGENERATION (Pathology) , *META-analysis , *ARTHROPLASTY , *REOPERATION , *CERVICAL vertebrae , *SPINAL fusion - Abstract
Background: This meta-analysis was designed to investigate the long-term efficacy and safety between cervical disc arthroplasty (CDA) and anterior cervical discectomy and fusion (ACDF) in treating cervical disc degenerative diseases (CDDDs).Methods: Literature search was performed on Pubmed, Embase, Cochrane Library, and Web of Science before Jan 2019. Surgical details, clinical outcomes, range of motion (ROM), complications, and reoperation rates between CDA and ACDF groups were compared and analyzed. A fixed- or random-effects model was applied based on different heterogeneity. STATA (Version 11.0) software was used to perform data analysis.Results: A total of 13 randomized controlled trial studies with more than 60 months of follow-up (mean 83.1 months) were enrolled in this meta-analysis. Pool results indicated that the CDA group exhibited significantly better outcomes in clinical scores (odds ratio [OR] = 1.54, 95% confidence interval [CI]: 1.15-2.08, p = 0.004) and preservation of ROM (mean difference = 1.77, 95% CI: 1.60-1.95, p < 0.001) than the ACDF group. Meanwhile, the incidence of adjacent segment disease (ASD) (OR = 0.51, 95% CI: 0.35-0.76, p = 0.001) and occurrence of reoperation (OR = 0.41, 95% CI: 0.25-0.69, p = 0.001) were lower in the CDA group than in the ACDF group.Conclusions: At long-term follow-up, CDA showed better efficacy in terms of clinical outcomes, ROM, ASD, and reoperation than ACDF for treating CDDDs. However, our results require further validation in large-sample and high-quality studies. [ABSTRACT FROM AUTHOR]- Published
- 2020
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20. Cover image.
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Gu, Chao, Zhou, Quan, Hu, Xiayu, Ge, Xiaoyang, Hou, Mingzhuang, Wang, Wenhao, Liu, Hao, Shi, Qin, Xu, Yong, Zhu, Xuesong, Yang, Huilin, Chen, Xi, Liu, Tao, and He, Fan
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MESENCHYMAL stem cells - Abstract
The cover image is based on the Original Article Melatonin rescues the mitochondrial function of bone marrow‐derived mesenchymal stem cells and improves the repair of osteoporotic bone defect in ovariectomized rats by Chao Gu et al., https://doi.org/10.1111/jpi.12924..By Chao Gu; Quan Zhou; Xiayu Hu; Xiaoyang Ge; Mingzhuang Hou; Wenhao Wang; Hao Liu; Qin Shi; Yong Xu; Xuesong Zhu; Huilin Yang; Xi Chen; Tao Liu and Fan HeReported by Author; Author; Author; Author; Author; Author; Author; Author; Author; Author; Author; Author; Author; Author [Extracted from the article]
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- 2024
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21. In situ silk fibroin-mediated crystal formation of octacalcium phosphate and its application in bone repair.
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Han, Fengxuan, Hu, Yuanbin, Li, Jiaying, Gong, Jiawei, Guo, Qianping, Zhu, Caihong, Zhu, Xuesong, Yang, Huilin, and Li, Bin
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SILK fibroin , *CALCIUM phosphate , *TISSUE scaffolds , *BONE substitutes , *BONE regeneration - Abstract
Abstract The development of an ideal scaffold material is critical for the repair of bone defects. Being an important precursor of the mineralized matrix of bone tissue, octacalcium phosphate (OCP) has been considered a promising bone substitute. However, its application is largely limited due to the thermodynamical instability and poor processability of it. In this study, OCP was prepared by co-precipitation in the presence of small amount of silk fibroin (SF), which regulated the crystallization of OCP and led to the formation of SF-OCP complex. The diameters of OCP crystals in OCP, 0.1SF-OCP, 0.3SF-OCP and 1SF-OCP complexes were 489.0 ± 399.1 nm, 102.2 ± 50.7 nm, 94.7 ± 48.4 nm and 223.7 ± 167.6 nm, respectively. However, the shape of OCP crystals did not apparently change by the presence of SF. Further, porous SF/OCP composite scaffolds with pore size of 111.9 ± 33.1 μm were prepared, in which small crystals of SF-OCP complex were embedded in a SF matrix. MC3T3-E1 cells could attach and proliferate well on both the rugged surfaces and the pores of SF/OCP scaffolds, indicating their decent biocompatibility. Further, SF/OCP scaffolds markedly promoted bone regeneration in a rat calvarial critical-sized defect model. Both micro-CT and H&E characterizations showed that bone formation not only occurred around the scaffolds, but also penetrated into their center. Therefore, such SF/OCP composite scaffolds may have potential applications in bone tissue engineering. Graphical abstract Unlabelled Image Highlights • Silk fibroin (SF) mediated crystallization of octacalcium phosphate (OCP) in a SF content-dependent manner. • SF/OCP scaffolds well supported cell adhesion and proliferation. • Effective new bone formation was achieved using SF/OCP scaffolds. [ABSTRACT FROM AUTHOR]
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- 2019
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22. Reprogramming of Mitochondrial Respiratory Chain Complex by Targeting SIRT3‐COX4I2 Axis Attenuates Osteoarthritis Progression (Adv. Sci. 10/2023).
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Zhang, Yijian, Liu, Yang, Hou, Mingzhuang, Xia, Xiaowei, Liu, Junlin, Xu, Yong, Shi, Qin, Zhang, Zhongmin, Wang, Liang, Shen, Yifan, Yang, Huilin, He, Fan, and Zhu, Xuesong
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MITOCHONDRIA , *OSTEOARTHRITIS , *SPACE stations - Abstract
B Mitochondrial Reprogramming in Osteoarthritis b The Mengtian and Wentian lab modules docked with Tianhe core module after entering the planned orbit. Just like the satellite docking for exploring cosmic mysteries, the activation of SIRT3-COX4I2 axis in mitochondria sheds new light on the pathogenesis of osteoarthritis. Reprogramming of Mitochondrial Respiratory Chain Complex by Targeting SIRT3-COX4I2 Axis Attenuates Osteoarthritis Progression (Adv. Sci. 10/2023). [Extracted from the article]
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- 2023
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23. Dynamic protein hydrogel with supramolecularly enveloped kartogenin promotes cartilage regeneration through mitochondrial activation.
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Zhang, Yijian, Yin, Weiling, Liu, Yang, Hou, Mingzhuang, Shi, Qin, Liu, Tao, Wang, Miao, Yang, Huilin, Pan, Guoqing, He, Fan, and Zhu, Xuesong
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CARTILAGE cells , *CARTILAGE regeneration , *HYDROGELS , *MITOCHONDRIA , *CHONDROGENESIS , *CYCLODEXTRINS , *ENDOCHONDRAL ossification - Abstract
Limited self-renewal potency of cartilage impedes the repair and regeneration at the site of damage. Investigating small-molecule compounds to boost chondrogenesis has yielded the dawn. However, the durability and targeting of current drug delivery and the adaptive integration for cartilage defects are not addressed. Herein, we fabricated a dynamic hydrogel based on the coordination between metal ions and collagen and then supramolecularly enveloped with a chondro-inductive factor kartogenin (KGN) via cyclodextrin (CD). The self-recovery and injectability of dynamic hydrogel enabled minimally invasive and precise treatment of cartilage injury assisted by continuous KGN administration. The hydrogel could significantly promote the expression of cartilage matrix components (type II collagen and aggrecan) in articular chondrocytes without affecting cell proliferation or migration in vitro. Mechanistically, the protective effects depended on enhancing chondrocyte mitochondrial function and intracellular antioxidant properties. In situ hydrogel injection strengthened cartilage regenerative potential and facilitated the reconstruction of subchondral bone following full-thickness defects in vivo. This study revealed that the dynamic chondrogenic hydrogel could benefit cartilage repair via mitochondrial reinforcement, acting as a novel therapeutic strategy for refractory cartilage damage. [ABSTRACT FROM AUTHOR]
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- 2022
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24. Pharmaceutical inhibition of glycogen synthetase kinase 3 beta suppresses wear debris-induced osteolysis.
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Geng, Dechun, Wu, Jian, Shao, Hongguo, Zhu, Shijun, Wang, Yijun, Zhang, Wen, Ping, Zichuan, Hu, Xuanyang, Zhu, Xuesong, Xu, Yaozeng, and Yang, Huilin
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BONE diseases , *BONE resorption , *GLYCOGEN synthase kinase-3 , *GENE expression , *LABORATORY mice , *PREVENTION ,TREATMENT of bone diseases - Abstract
Aseptic loosening is associated with the development of wear debris-induced peri-implant osteolytic bone disease caused by an increased osteoclastic bone resorption and decreased osteoblastic bone formation. However, no effective measures for the prevention and treatment of peri-implant osteolysis currently exist. The aim of this study was to determine whether lithium chloride (LiCl), a selective inhibitor of glycogen synthetase kinase 3 beta (GSK-3β), mitigates wear debris-induced osteolysis in a murine calvarial model of osteolysis. GSK-3β is activated by titanium (Ti) particles, and implantation of Ti particles on the calvarial surface in C57BL/6 mice resulted in osteolysis caused by an increase in the number of osteoclasts and a decrease in the number of osteoblasts. Mice implanted with Ti particles were gavage-fed LiCl (50 or 200 mg kg −1 d −1 ), 6 days per week for 2 weeks. The LiCl treatment significantly inhibited GSK-3β activity and increased β-catenin and axin-2 expression in a dose-dependent manner, dramatically mitigating the Ti particle-induced suppression of osteoblast numbers and the expression of bone formation markers. Finally, we demonstrated that inhibition of GSK-3β suppresses osteoclast differentiation and reduces the severity of Ti particle-induced osteolysis. The results of this study indicate that Ti particle-induced osteolysis is partly dependent on GSK-3β and, therefore, the canonical Wnt signaling pathway. This suggests that selective inhibitors of GSK-3β such as LiCl may help prevent and treat wear debris-induced osteolysis. [ABSTRACT FROM AUTHOR]
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- 2015
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25. Promoting the cyclic and rate performance of nickel hydroxide with ZnO via electrodeposition for supercapacitor.
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Zhang, Meng, Zang, Ruxin, Zhang, Mengmeng, Liu, Rui, Zhu, Xuesong, Li, Xinbo, Cui, Hongzhi, and Zhu, Huiling
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NICKEL sulfide , *ELECTROPLATING , *ZINC oxide , *HYDROXIDES , *ELECTRON transport , *ENERGY density , *POWER density - Abstract
A novel Ni(OH) 2 /ZnO hydrangea-like structure is prepared via a facile electrodeposition strategy with the Ni(OH) 2 flower-like particles growing on the ZnO layered carrier. This flower-like structure can take advantage of both ZnO and Ni(OH) 2 , leading to a fast ion/electron transport speed and structural stability, finally, attaining an outstanding specific capacitance (2264.55 F g−1 at 0.5 A g−1), excellent rate performance (601.09 F g−1 at 30 A g−1) and cycle stability (maintain 94.9% specific capacitance after 5000 cycles). The Ni-OH/ZnO//AC asymmetric supercapacitor device achieves a high energy density of 68.44 Wh kg−1 at a power density of 400 W kg−1, demonstrating great potential as an electrode material for energy storage devices. The mechanism of synergistic effect of Ni(OH) 2 and ZnO mutually supporting composite structure to improve electrochemical properties is innovatively proposed. • A novel Ni(OH) 2 /ZnO hydrangea-like structure is prepared via electrodeposition. • Outstanding cyclic and rate performance is achieved due to the supporting of ZnO. • The composite fulfills an outstanding capacitance of 2264.55 F g−1 at 0.5 A g−1. • The synergistic effect of mutually supporting structure is discussed in detail. [ABSTRACT FROM AUTHOR]
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- 2022
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26. Melatonin: A novel candidate for the treatment of osteoarthritis.
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Zhang, Yijian, Liu, Tao, Yang, Huilin, He, Fan, and Zhu, Xuesong
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CARTILAGE cells , *CHONDROGENESIS , *OSTEOARTHRITIS , *MELATONIN , *ARTICULAR cartilage , *CARTILAGE regeneration , *KNEE , *CARTILAGE - Abstract
Osteoarthritis (OA), characterized by cartilage erosion, synovium inflammation, and subchondral bone remodeling, is a common joint degenerative disease worldwide. OA pathogenesis is regulated by multiple predisposing factors, including imbalanced matrix metabolism, aberrant inflammatory response, and excessive oxidative stress. Moreover, melatonin has been implicated in development of several degenerative disorders owing to its potent biological functions. With regards to OA, melatonin reportedly promotes synthesis of cartilage matrix, inhibition of chondrocyte apoptosis, attenuation of inflammatory response, and suppression of matrix degradation by regulating the TGF-β, MAPK, or NF-κB signaling pathways. Notably, melatonin has been associated with amelioration of oxidative damage by restoring the OA-impaired intracellular antioxidant defense system in articular cartilage. Findings from preliminary application of melatonin or melatonin-loaded biomaterials in animal models have affirmed its potential anti-arthritic effects. Herein, we summarize the anti-arthritic effects of melatonin on OA cartilage and demonstrate that melatonin has potential therapeutic efficacy in treating OA. Melatonin reverses the progression of OA and boosts cartilage recovery at multiple dimensions. At the organismal level, intra-articular injection of melatonin suppresses arthritis development and promotes cartilage regeneration. At the tissue level, melatonin modulates cartilage erosion, synovial inflammation, subchondral bone remodeling, and thus impedes cartilage degeneration. At the cellular level, chondrocyte apoptosis, inflammatory cytokines (e.g., IL-1β or TNF-α), anabolic/catabolic activities (e.g., MMPs or ADAMTS versus COLII or ACAN), and oxidative stress status are harmonized by melatonin treatment. At the organelle level, melatonin preserves matrix metabolic and redox equilibrium via enhancement of mitochondrial function, regulation of sirtuins activities, scavenging of free radicals as well as reinforcement of antioxidant properties (e.g., SOD, CAT, or GPX), through targeting the MAPK, NF-κB, TGF-β, NRF2, or FOXO signaling pathways. [Display omitted] • Melatonin protects articular chondrocytes from OA-induced cellular apoptosis. • Melatonin improves cartilage ECM synthesis and suppresses catabolic metabolism. • Melatonin maintains redox homeostasis via regulating oxidant–antioxidant balance. • Melatonin regulates the activities of NAD+-dependent deacylases sirtuins. • Intra-articular delivery of melatonin-laden biomaterials ameliorates cartilage degeneration. [ABSTRACT FROM AUTHOR]
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- 2022
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27. Platelet-Rich Plasma Inhibits Intervertebral Disc Degeneration via TGF-β1/Smad Pathway.
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Zou, Jun, Yang, Huilin, and Zhu, Xuesong
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BLOOD platelets , *DEGENERATION (Pathology) , *BLOOD plasma , *INTERVERTEBRAL disk diseases , *TRANSFORMING growth factors-beta - Published
- 2014
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28. Is balloon kyphoplasty better than percutaneous vertebroplasty for osteoporotic vertebral biconcave-shaped fractures?
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Gan, Minfeng, Zou, Jun, Song, Dawei, Zhu, Xuesong, Wang, Genlin, and Yang, Huilin
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VERTEBROPLASTY , *BONE fractures , *OSTEOPOROSIS , *BONE diseases , *WOUNDS & injuries - Abstract
Background: Osteoporotic vertebral biconcave-shaped fractures are not commonly seen in clinical practice. Some articles have been published showing the outcome of vertebroplasty (PV) and balloon kyphoplasty (BKP), but few comparative studies have been performed.Purpose: To compare the effect and safety of PV and BKP in treating osteoporotic vertebral biconcave-shaped fractures.Material and Methods: In this retrospective comparative study, 38 patients with osteoporotic vertebral biconcave-shaped fractures were treated by PV, and 41 patients were treated by BKP from May 2005 to July 2011. The heights of the compromised vertebral body and the kyphotic angles were measured. Visual analogue scale (VAS) and Oswestry Disability Index (ODI) were used to evaluate pain and functional activity, respectively. The occurrence of refracture and cement leakage were determined, and the costs were recorded.Results: The mean VAS and ODI scores significantly improved for both procedures at postsurgical measurements (P < 0.05), and the improvement sustained at the final follow-up. In both groups, there were no significant differences in terms of restoration of the anterior vertebral height and correction of the kyphotic deformity. However, BKP was more effective in restoring the middle vertebral height than PV. Cement leakages were observed in nine (23.7%) treated vertebral bodies in PV group and in three (7.4%) treated vertebral bodies in BKP group, which was a statistically significant difference (P < 0.05). There were four new osteoporotic vertebral fractures in the PV group and two in the BKP group during the follow-up period. The mean cost in the BKP group (6200 ± 122.1 USD) was higher than the PV group (2100 ± 112.5 USD) (P < 0.05).Conclusion: Both PV and BKP achieved similar improvements in pain and functional outcomes for the treatment of osteoporotic vertebral biconcave-shaped fractures. BKP had a significant advantage over PV in terms of the restoration of the middle vertebral height and fewer cement leakages than PV. [ABSTRACT FROM AUTHOR]- Published
- 2014
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29. Accelerated Postero-Lateral Spinal Fusion by Collagen Scaffolds Modified with Engineered Collagen-Binding Human Bone Morphogenetic Protein-2 in Rats.
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Han, Xinglong, Zhang, Wen, Gu, Jun, Zhao, Huan, Ni, Li, Han, Jiajun, Zhou, Yun, Gu, Yannan, Zhu, Xuesong, Sun, Jie, Hou, Xianglin, Yang, Huilin, Dai, Jianwu, and Shi, Qin
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POSTEROLATERAL corner , *SPINAL fusion , *TISSUE scaffolds , *COLLAGEN-binding proteins , *BONE morphogenetic proteins , *LABORATORY rats , *OSTEOINDUCTION - Abstract
Bone morphogenetic protein-2 (BMP-2) is a potent osteoinductive cytokine that plays a critical role in bone regeneration and repair. However, its distribution and side effects are major barriers to its success as therapeutic treatment. The improvement of therapy using collagen delivery matrices has been reported. To investigate a delivery system on postero-lateral spinal fusion, both engineered human BMP-2 with a collagen binding domain (CBD-BMP-2) and collagen scaffolds were developed and their combination was implanted into Sprague-Dawley (SD) rats to study Lumbar 4–5 (L4–L5) posterolateral spine fusion. We divided SD rats into three groups, the sham group (G1, n = 20), the collagen scaffold-treated group (G2, n = 20) and the BMP-2-loaded collagen scaffolds group (G3, n = 20). 16 weeks after surgery, the spines of the rats were evaluated by X-radiographs, high-resolution micro-computed tomography (micro-CT), manual palpation and hematoxylin and eosin (H&E) staining. The results showed that spine L4–L5 fusions occurred in G2(40%) and G3(100%) group, while results from the sham group were inconsistent. Moreover, G3 had better results than G2, including higher fusion efficiency (X score, G2 = 2.4±0.163, G3 = 3.0±0, p<0.05), higher bone mineral density (BMD, G2: 0.3337±0.0025g/cm3, G3: 0.4353±0.0234g/cm3. p<0.05) and more bone trabecular formation. The results demonstrated that with site-specific collagen binding domain, a dose of BMP-2 as low as 0.02mg CBD-BMP-2/cm3 collagen scaffold could enhance the posterolateral intertransverse process fusion in rats. It suggested that combination delivery could be an alternative in spine fusion with dramatically decreased side effects caused by high dose of BMP-2. [ABSTRACT FROM AUTHOR]
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- 2014
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30. Boosting energy and power of Cu-doped NiCo2S4/graphite electrode for high performance supercapacitors.
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Zhang, Mengmeng, Zheng, Hao, Zhu, Huiling, Zhang, Meng, Liu, Rui, Zhu, Xuesong, Li, Xinbo, and Cui, Hongzhi
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SUPERCAPACITOR electrodes , *ELECTRODE performance , *SUPERCAPACITORS , *ENERGY density , *ENERGY storage , *CARBON electrodes , *GRAPHITE composites - Abstract
• The 5% Cu-NCS@FG exhibits mesoporous structure for electrolyte ions diffusion. • A small amount of Cu doping can completely maintain the 3D structure of NCS. • An outstanding rate capability of 86% at 20 times current density can be achieved. • The synergy effect of Cu and FG significantly enhances the electrochemical property. Cu-doped NiCo 2 S 4 /flake graphite nanosheet arrays were prepared by multi-step hydrothermal calcination. Three-dimensional structure provides a wealth of active sites and structure stability to improve the charging/discharging rate. Moreover, 5% Cu-doped NiCo 2 S 4 /flake graphite composite fulfills an excellent specific capacitance of 3080 F·g−1 at 1 A·g−1 and remains 2600 F·g−1 at 20 A·g−1 with an outstanding capacitance retention rate of 86% at 20 times current density. The all-solid supercapacitor with 5% Cu-doped NiCo 2 S 4 /flake graphite electrode and activated carbon electrode exhibits an excellent energy density of 79.4 Wh·g−1 at a power density of 400 W·kg−1 with an outstanding cycling stability of 94% retention after 10000 cycles,suggesting its great potential for the development of high-performance energy storage devices. [ABSTRACT FROM AUTHOR]
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- 2022
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31. Osteoporotic vertebral fractures without compression: key factors of diagnosis and initial outcome of treatment with cement augmentation.
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Mao, Haiqing, Zou, Jun, Geng, Dechun, Zhu, Xuesong, Zhu, Mo, Jiang, Weimin, and Yang, Huilin
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EARLY diagnosis , *BONE cements , *BACKACHE , *COMPARATIVE studies , *ENDOSCOPIC surgery , *BONE fractures , *PATIENT aftercare , *MAGNETIC resonance imaging , *OSTEOPOROSIS , *HEALTH outcome assessment , *PATIENTS , *POSTOPERATIVE period , *QUESTIONNAIRES , *SPINAL surgery , *SPINAL injuries , *SURGERY , *T-test (Statistics) , *VISUAL analog scale , *TREATMENT effectiveness , *RETROSPECTIVE studies , *PREOPERATIVE period , *DATA analysis software , *DESCRIPTIVE statistics , *DISEASE complications , *THERAPEUTICS - Abstract
Introduction: Because of the nonspecific symptoms and unapparent radiographic findings, occult osteoporotic vertebral fractures (VFs) have a high rate of missed diagnosis, and the treatment of these fractures has rarely been discussed in the literature. We investigated diagnostic key factors of such occult VFs and evaluated the effects of cement augmentation in these patients. Methods: A total of 225 patients with VFs treated with vertebroplasty (VP) or kyphoplasty (KP) were retrospectively analyzed. All patients have taken preoperatively magnetic resonance imaging (MRI) including T1/T2-weighted images and short tau inversion recovery (STIR) sequences, and 45 patients met the inclusion criteria. Clinical outcomes were evaluated by comparing visual analog scale (VAS) and Oswestry disability index (ODI) values preoperatively, postoperatively, and at the final follow-up. Results: MRI (T1 and STIR sequences) showed bone marrow edema in all fresh fractures including occult VFs and concomitant VFs; five patients showed no abnormality signal in their T2-weighted sequences of occult VFs. While 40 patients underwent KP, and five patients underwent VP. We did not find delayed collapsed fractures in the augmented occult VFs in both groups after operation. Both groups had significant improvement in pain and functional activity after the intervention ( p<0.01). Conclusion: The presence of previous or concomitant VFs leads to further imaging examination, which helps identify the occult VFs. Preoperative MRI examination especially T1 and STIR sequences plays a vital role in the early diagnosis of occult VFs. Except for significant pain relief and functional improvement, VP and KP can effectively prevent delayed vertebral collapse of occult VFs comparing to previous reported data. [ABSTRACT FROM AUTHOR]
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- 2012
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32. A Novel Cell-Based Therapy in Segmental Bone Defect: Using Adipose Derived Stromal Cells
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Zou, Jun, Wang, Genlin, Geng, Dechun, Zhu, Xuesong, Gan, Minfeng, and Yang, Huilin
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BONE abnormalities , *CELLULAR therapy , *BONE marrow , *CONNECTIVE tissue cells , *CELL culture , *OSTEOPONTIN , *BONE growth , *LABORATORY rabbits , *THERAPEUTICS - Abstract
Background: Cell-based therapy is currently focusing on bone marrow as an ideal source. However, harvesting of bone marrow is always associated with some potential morbidity. It was recently revealed that adipose derived stromal cells contain a cell population with multipotency. In addition to an adequate supply, these cells do not appear to decline with age. The purpose of our study was to assess osteogenic capability of these cells in vitro and in vivo. Materials and Methods: Adipose derived stromal cells were isolated from New Zealand white rabbit fat pads. After primary culture in basic medium and expanded to two passages, the cells were cultured in an osteogenic medium for 2–4 wk to induce osteogenesis. In vivo, segmental bone defects were created at left radius in 30 rabbits. The cultured cells were implanted into the defects through open operation. Results: Adipose derived stromal cells were able to be induced to osteogenesis confirmed by ALP and von Kossa staining. Some specific markers, such as ALP, osteopontin, osteocalcin, were also detected by PCR. The segmental defects had complete union, judged by radiographic analysis and histologic analysis, in the group transplanted with these cells. Conclusion: Adipose derived stromal cells have the potential to differentiate into osteogenic lineage both in vitro and in vivo. It would be a promising novel cell-based therapy for healing bone defects in the clinical setting. [ABSTRACT FROM AUTHOR]
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- 2011
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33. Sensitization of Osteosarcoma Cell Line SaOS-2 to Chemotherapy by Downregulating Survivin
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Zou, Jun, Gan, Minfeng, Mao, Nana, Zhu, Xuesong, Shi, Qin, and Yang, Huilin
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OSTEOSARCOMA , *CANCER chemotherapy , *GENETIC regulation , *CELL lines , *FLOW cytometry , *APOPTOSIS , *MOLECULAR oncology , *THERAPEUTICS - Abstract
Background and Aims: Osteosarcoma is the most frequent malignant bone tumor with a peak incidence in the second and third decades of life. Survivin, a member of the IAP family of proteins, is overexpressed in osteosarcomas and plays an important role in protecting cells from apoptosis. Here we investigated the anti-cancer effects of downregulating survivin by shRNA vector pSUPER-sh in combination with chemotherapeutic drugs on human osteosarcoma cells. Methods: Expression of survivin was detected by Western blot. The effects of pSUPER-sh and chemotherapeutic drugs on osteosarcoma cell lines Saos-2 and U2OS by cell viability assay and its underlying mechanisms were analyzed by flow cytometry and caspase-3 activity assay. Results: Downregulated survivin could significantly induce apoptosis of osteosarcoma cell lines Saos-2 and U2OS. The effect probably resulted from downregulation of survivin induced by pSUPER-sh. Importantly, we found that the downregulation of survivin by pSUPER-sh could enhance the anticancer effects of chemotherapies such as etoposide, cisplatin and doxorubicin through decreasing mitochondrial membrane potentials and increasing caspase-3 activity. Conclusions: Downregulated survivin by pSUPER-sh could markedly induce apoptosis of osteosarcoma cells lines and pSUPER-sh may be a promising adjuvant in osteosarcoma chemotherapy. [Copyright &y& Elsevier]
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- 2010
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34. Kyphoplasty for chronic painful osteoporotic vertebral compression fractures via unipedicular versus bipedicular approachment: A comparative study in early stage
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Chen, ChunMao, Chen, Liang, Gu, Yong, Xu, Yun, Liu, Yong, Bai, XiaoLiang, Zhu, XueSong, and Yang, HuiLin
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COMPRESSION fractures , *OSTEOPOROSIS , *CHRONIC pain , *TREATMENT effectiveness , *COMPARATIVE studies ,TREATMENT of spine diseases - Abstract
Abstract: Background: Osteoporotic compression fractures (OVCFs) commonly occur in aged people, and as much as one-third of these fractures progress to chronic pain. Kyphoplasty (KP) is proved to be efficacious for pain relief and vertebral height restoration in chronic OVCFs, but there is still no data available about the clinical and radiographical outcomes compared by unipedicular and bipedicular KP in treating chronic painful OVCFs. Purpose: To assess the clinical and radiographical outcomes in treating chronic painful OVCFs compared by unipedicular and bipedicular KP. Methods: Fifty-eight patients with a total of sixty-six chronic painful OVCFs were enroled in our study. They were randomly allocated into two groups: group I (n =33) was treated with unipedicular KP and group II (n =25) with bipedicular KP. The operation times for each group were recorded and compared. Preoperative and postoperative of visual analogue scores (VAS) and oswestry disability index (ODI) scores were compared 2 weeks after surgery within each group and between groups. The radiographic outcomes were evaluated by the restoration rate (RR) in the most compressed point of the vertebral bodies. Results: Significant improvement on the VAS, ODI scores and RR was noted in each group (p <0.001), and there is no significant difference existing in clinical outcomes between the two groups. The mean operation time for each vertebra in group I was significantly shorter than in group II (p <0.001). But the RR in group II was higher than in group I (p =0.041). Conclusion: Both unipedicular kyphoplasty and bipedicular kyphoplasty can achieve satisfactory clinical and radiographic outcomes in treating the chronic painful OVCFs and the operation time is shorter in unipedicular kyphoplasty. However, the bipedicular kyphoplasty is more efficacious in height restoration. [Copyright &y& Elsevier]
- Published
- 2010
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35. Protection against titanium particle induced osteolysis by cannabinoid receptor 2 selective antagonist
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Geng, Dechun, Xu, Yaozeng, Yang, Huilin, Wang, Junhua, Zhu, Xuesong, Zhu, Guangming, and Wang, Xianbin
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TITANIUM , *BONE resorption , *CANNABINOIDS , *ARTHROPLASTY , *LABORATORY mice , *GENE expression - Abstract
Abstract: Osteolysis and subsequent aseptic loosening are the most common causes of failure of total joint arthroplasty. Osteolysis is initiated by inflammatory response to wear debris, resulting in localized, osteoclastic peri-implant bone loss. However, there were no effective measures for prevention and treatment of periprosthetic osteolysis. The aim of the current study was to determine whether CB2 selective antagonist (AM630) inhibits wear debris-induced osteolysis in a murine osteolysis model. Titanium (Ti) particles were introduced into established air pouches on BALB/c mice, followed by implantation of calvaria bone from syngeneic littermates. AM630 was given to mice intraperitoneally 2 days before Ti particles introduction and maintained until the sacrifice of the mice. Mice without drug treatment, as well as mice injected with saline alone, were included. Each group contains 10 mice. Pouch tissues were harvested 14 days after bone implantation for histological and molecular analysis. Ti particles stimulation significantly increased CB2 expression. However, less CB2 was observed in AM630 treatment group. AM630 inhibited Ti particle-induced osteolysis associated gene activity of RANK, RANKL and CPK, and diminished RANKL expression in Ti particle stimulated pouches. AM630 markedly reduced the number of TRAP+ cells in pouch tissues. In conclusion, this study provides the evidence that blockage of CB2 with AM630 can markedly reduce Ti particle induced osteolysis in a murine air pouch model. This finding points to the possibility that CB2 selective antagonists like AM630 may have potential value for prevention and treatment of wear particle induced osteolysis. [Copyright &y& Elsevier]
- Published
- 2010
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36. Deletion of SIRT3 inhibits osteoclastogenesis and alleviates aging or estrogen deficiency-induced bone loss in female mice.
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Zhang, Yijian, He, Fan, Yang, Huilin, and Zhu, Xuesong
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AGING , *OSTEOCLASTOGENESIS , *MICE , *FEMALES , *ESTROGEN - Published
- 2021
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37. Immunopolarization-regulated 3D printed-electrospun fibrous scaffolds for bone regeneration.
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Liu, Xingzhi, Chen, Mimi, Luo, Junchao, Zhao, Huan, Zhou, Xichao, Gu, Qiaoli, Yang, Huilin, Zhu, Xuesong, Cui, Wenguo, and Shi, Qin
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PI3K/AKT pathway , *IMMUNE response , *PHENOTYPES , *BONE regeneration , *MACROPHAGES , *NEOVASCULARIZATION , *BONE growth - Abstract
Three-dimension (3D)-printed bioscaffolds are precise and personalized for bone regeneration. However, customized 3D scaffolds may activate the immune response in vivo and consequently impede bone formation. In this study, with layer-by-layer deposition and electrospinning technology to control the physical structure, 3D-printed PCL scaffolds with PLLA electrospun microfibrous (3D-M-EF) and nanofibrous (3D-N-EF) composites were constructed, and their immunomodulatory effect and the subsequent osteogenic effects were explored. Compared to 3D-N-EF scaffolds, 3D-M-EF scaffolds polarized more RAW264.7 cells toward alternatively activated macrophages (M2), as demonstrated by increased M2 and deceased classically activated macrophage (M1) phenotypic marker expression in the cells. In addition, the 3D-M-EF scaffolds shifted RAW264.7 cells to the M2 phenotype through PI3K/AKT signaling and enhanced VEGF and BMP-2 expression. Conditional medium from the RAW264.7 cells seeded in 3D-M-EF scaffolds promoted osteogenesis of MC3T3-E1 cells. Furthermore, in vivo study of repairing rat calvarial defects, the 3D-M-EF scaffolds increased the polarization of M2 macrophages, enhanced angiogenesis, and accelerated new bone formation. Collectively, our data suggested that well-designed 3D-M-EF scaffolds are favorable for osteogenesis through regulation of M2 polarization. Therefore, it is potential to utilize the physical structure of 3D-printed scaffolds to manipulate the osteoimmune environment to promote bone regeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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38. A selective CB2 agonist protects against the inflammatory response and joint destruction in collagen-induced arthritis mice.
- Author
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Wang, Mingjun, Geng, Dechun, Bai, Jiaxiang, Ge, Gaoran, Wang, Yijun, Zhang, Wenhao, Wang, Qing, Wang, Wei, Guo, Xiaobin, Yu, Binqing, Xu, Yaozeng, Yang, Huilin, and Zhu, Xuesong
- Subjects
- *
NITRIC-oxide synthases , *ARTHRITIS , *ETIOLOGY of diseases , *CANNABINOID receptors , *RHEUMATOID arthritis - Abstract
Rheumatoid arthritis (RA) is a chronic, inflammatory, synovitis-dominated systemic disease with unknown etiology. RA is characterized by the involvement of multiple affected joints, symmetry, and invasive arthritis of the limbs, which can lead to joint deformity, cartilage destruction, and loss of function. Cannabinoid receptor 2 (CB 2) has potent immunomodulatory and anti-inflammatory effects and is predominantly expressed in non-neuronal tissues. In the current study, the role of CB 2 in the process of inflammatory bone erosion in RA was examined. The selective agonist or high-affinity ligand of CB 2 (4-quinolone-3-carboxamides CB2 agonist, 4Q3C CB 2 agonist, 4Q3C) significantly reduced the severity of arthritis, decreased histopathological findings, and markedly reduced bone erosion in collagen-induced arthritis (CIA) mice. In addition, 4Q3C prevented an increase in the nuclear factor-κB ligand (RANKL)/osteoprotegerin (OPG) ratio and inhibited the formation of osteoclasts in CIA mice. Furthermore, the expression of tumor necrosis factor-alpha, interleukin-1β, cyclooxygenase-2, and inducible nitric oxide synthase was lower in 4Q3C-treated CIA mice than in control CIA mice. Micro-computed tomography corroborated the finding that 4Q3C reduced joint destruction. These data clearly indicate that the CB 2 -selective agonist, 4Q3C, may have anti-inflammatory and anti-osteoclastogenesis effects in RA and may be considered to be a novel treatment for RA. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
39. Multifunctional Coating to Simultaneously Encapsulate Drug and Prevent Infection of Radiopaque Agent.
- Author
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Li, Jiaying, Wang, Huan, Guo, Qianping, Zhu, Caihong, Zhu, Xuesong, Han, Fengxuan, Yang, Huilin, and Li, Bin
- Subjects
- *
POLYMETHYLMETHACRYLATE , *RADIOGRAPHIC contrast media , *BIOCOMPATIBILITY , *ESCHERICHIA coli , *BACTERIAL diseases , *ANTIBACTERIAL agents - Abstract
Poly(methyl methacrylate) (PMMA) bone cements have been widely used in clinical practices. In order to enhance PMMA's imaging performance to facilitate surgical procedures, a supplementation of radiopaque agent is needed. However, PMMA bone cements are still facing problems of loosening and bacterial infection. In this study, a multifunctional coating to simultaneously encapsulate drug and prevent the infection of radiopaque agent has been developed. Barium sulfate (BaSO4), a common radiopaque agent, is used as a substrate material. We successfully fabricated porous BaSO4 microparticles, then modified with hexakis-(6-iodo-6-deoxy)-alpha-cyclodextrin (I-CD) and silver (Ag) to obtain porous BaSO4@PDA/I-CD/Ag microparticles. The porous nature and presence of PDA coating and I-CD on the surface of microparticles result in efficient loading and release of drugs such as protein. Meanwhile, the radiopacity of BaSO4@PDA/I-CD/Ag microparticles is enhanced by this multifunctional coating containing Ba, I and Ag. PMMA bone cements containing BaSO4@PDA/I-CD/Ag microparticles show 99% antibacterial rate against both Staphylococcus aureus (S. aureus) and Escherichia Coli (E. coli), yet without apparently affecting its biocompatibility. Together, this multifunctional coating possessing enhanced radiopacity, controlled drug delivery capability and exceptional antibacterial performance, may be a new way to modify radiopaque agents for bone cements. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
40. The “Magnesium Sacrifice” Strategy Enables PMMA Bone Cement Partial Biodegradability and Osseointegration Potential.
- Author
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Zhai, Qingpan, Han, Fengxuan, He, Zhiwei, Shi, Chen, Zhou, Pinghui, Zhu, Caihong, Guo, Qianping, Zhu, Xuesong, Yang, Huilin, and Li, Bin
- Subjects
- *
METHYL methacrylate , *BIOCOMPATIBILITY , *BONE cements , *MICROSPHERES , *DRUG carriers - Abstract
Poly (methyl methacrylate) (PMMA)-based bone cements are the most commonly used injectable orthopedic materials due to their excellent injectability and mechanical properties. However, their poor biocompatibility and excessive stiffness may cause complications such as aseptic implant loosening and stress shielding. In this study, we aimed to develop a new type of partially biodegradable composite bone cement by incorporating magnesium (Mg) microspheres, known as “Mg sacrifices” (MgSs), in the PMMA matrix. Being sensitive to the physiological environment, the MgSs in PMMA could gradually degrade to produce bioactive Mg ions and, meanwhile, result in an interconnected macroporous structure within the cement matrix. The mechanical properties, solidification, and biocompatibility, both in vitro and in vivo, of PMMA–Mg bone cement were characterized. Interestingly, the incorporation of Mg microspheres did not markedly affect the mechanical strength of bone cement. However, the maximum temperature upon setting of bone cement decreased. This partially biodegradable composite bone cement showed good biocompatibility in vitro. In the in vivo study, considerable bony ingrowth occurred in the pores upon MgS degradation. Together, the findings from this study indicate that such partially biodegradable PMMA–Mg composite may be ideal bone cement for minimally invasive orthopedic surgeries such as vertebroplasty and kyphoplasty. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
41. 80. Osteogenic Differentiation of Adipose Derived Stromal Cells Induction of Spinal Fusion in a Rodent Model
- Author
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Zou, Jun, Yang, Huilin, Geng, Dechun, Zhu, Xuesong, Liu, Ling, Miyazaki, Masashi, and Wang, Jeffrey
- Published
- 2008
- Full Text
- View/download PDF
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