1. PirB-Cry2Aa hybrid protein exhibits enhanced insecticidal activity against Spodoptera exigua larvae.
- Author
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Xiaofeng Hu, Zhengqiang Liu, Yusheng Li, Xuezhi Ding, Liqiu Xia, and Shengbiao Hu
- Subjects
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INSECTICIDES , *BEET armyworm , *PHOTORHABDUS luminescens , *BACILLUS thuringiensis , *CHIMERIC proteins , *PHYSIOLOGICAL control systems , *HELICOVERPA armigera - Abstract
Previous study revealed that the N-terminal region of PirB toxin from Photorhabdus luminescens showed 20.5% identity and 41.5% similarity to the domain I of Cry2A toxin of Bacillus thuringiensis. The encoding sequence of the domain I of Cry2Aa protein was replaced by the encoding gene of corresponding domain of PirB protein. Expression of pirB-cry2Aa chimeric gene in B. thuringiensis acrystalliferous strain Cry-B leads to the formation of crystals with irregular shape. Bioassay showed that PirB-Cry2Aa hybrid protein displayed toxicity against Spodoptera exigua and Helicoverpa armigera larvae. Our data implied that PirB protein might possess pore-forming activity and PirB-Cry2Aa hybrid protein could be used as biological control agent. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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