1. A sample-to-answer, real-time convective polymerase chain reaction system for point-of-care diagnostics.
- Author
-
Shu, Bowen, Zhang, Chunsun, and Xing, Da
- Subjects
- *
POINT-of-care testing , *POLYMERASE chain reaction , *FLUORESCENCE spectroscopy , *VANCOMYCIN , *MOLECULAR diagnosis - Abstract
Timely and accurate molecular diagnostics at the point-of-care (POC) level is critical to global health. To this end, we propose a handheld convective-flow real-time polymerase chain reaction (PCR) system capable of direct sample-to-answer genetic analysis for the first time. Such a system mainly consists of a magnetic bead-assisted photothermolysis sample preparation, a closed-loop convective PCR reactor, and a wireless video camera-based real-time fluorescence detection. The sample preparation exploits the dual functionality of vancomycin-modified magnetic beads (VMBs) for bacteria enrichment and photothermal conversion, enabling cell pre-concentration and lysis to be finished in less than 3 min. On the presented system, convective thermocycling is driven by a single-heater thermal gradient, and its amplification is monitored in real-time, with an analysis speed of less than 25 min, a dynamic linear range from 10 6 to 10 1 copies/µL and a detection sensitivity of as little as 1 copies/µL. Additionally, the proposed PCR system is self-contained with a control electronics, pocket-size and battery-powered, providing a low-cost genetic analysis in a portable format. Therefore, we believe that this integrated system may become a potential candidate for fast, accurate and affordable POC molecular diagnostics. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF