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The sapwood of Japanese cedar ( Cryptomeria japonica D. Don ) was decayed by the brown-rot fungus Fomitopsis palustris under bright and dark conditions. Scanning electron microscopy revealed the presence of mycelia inside the wood even after 1 week from the start of fungal exposure. Moreover, holes were observed in the torus after fungal exposure. Ruthenium red staining revealed that the pectin in pits was largely absent for 3 weeks. These events occurred before the mass loss of wood samples was confirmed at the early stage. Moreover, Fp PG28A was more highly expressed at the hyphal front on a pectin-containing medium under dark conditions compared with bright conditions. This up-regulation under dark conditions indicated that the pectin decomposition ability was promoted inside the wood where light could not reach. In conclusion, we suggest that the brown-rot fungus completed its hyphal expansion within the wood via pectin decomposition in pits before holocellulose decomposition. [ABSTRACT FROM AUTHOR]

Tropical instability waves (TIWs) are prominent features in the equatorial Pacific, propagating westward at a speed of ~0.5ms-1 with a wavelength of ~1000 km. In this study, we show that a linear stability analysis using a 1.5-layer shallow water model can predict successfully an unstable mode whose wavelength, phase speed, growth rate, and meridional structure are all consistent with those of the TIWs simulated by an eddyresolving ocean general circulation model (OGCM). This unstable mode can be interpreted as resulting from the coupling of two Rossby waves, namely, one trapped just north of the equator (~1°-3.5°N) and the other trapped farther north (~3.5°-8°N). Although these two Rossby waves have opposite intrinsic phase propagation directions reflecting the negative and positive local meridional potential vorticity (PV) gradients, respectively, their actual propagation direction can be adjusted through the advection by the South Equatorial Current and the North Equatorial Countercurrent such that they might propagate westward at the same speed and with the same zonal wavenumber yielding the largest growth rate of TIWs. The unstable mode does not appear during the period in which the negative PV gradient is absent, which demonstrates its essential role in generating TIWs. Indeed, the seasonal and interannual variability of the TIWs simulated by the OGCM is shown to be significantly controlled by the strength of the negative PV gradient just north of the equator, suggesting that it could be a key parameter toward a dynamically based parameterization of the heat and momentum transfer associated with TIWs in coarse-resolution OGCMs. [ABSTRACT FROM AUTHOR]

We recently reported that NF-κB--mediated inflammation caused by breakpoint cluster region (BCR) is dependent on the a subunit of casein kinase II (CK2#945;) complex. In the current study, we demonstrate that presenilin 1 (Psen1), which is a catalytic component of the #947;-secretase complex and the mutations of which are known to cause familial Alzheimer disease, acts as a scaffold of the BCR--CK2#945;--p65 complex to induce NF-#954;B activation. Indeed, Psen1 deficiency in mouse endothelial cells showed a significant reduction of NF-#954;B p65 recruitment to target gene promoters. Conversely, Psen1 overexpression enhanced reporter activation under NF-#954;B responsive elements and IL-6 promoter. Furthermore, the transcription of NF-κB target genes was not inhibited by a #947;-secretase inhibitor, suggesting that Psen1 regulates NF-#954;B activation in a manner independent of #947;-secretase activity. Mechanistically, Psen1 associated with the BCR--CK2#945; complex, which is required for phosphorylation of p65 at serine 529. Consistently, TNF-#945;--induced phosphorylation of p65 at serine 529 was significantly decreased in Psen1-deficient cells. The association of the BCR--CK2#945;--p65 complex was perturbed in the absence of Psen1. These results suggest that Psen1 functions as a scaffold of the BCR--CK2#945;--p65 complex and that this signaling cascade could be a novel therapeutic target for various chronic inflammation conditions, including those in Alzheimer disease. [ABSTRACT FROM AUTHOR]

In Japan, legalized casinos have been developed through the enactment of the Integrated Resort Promotion Bill. In this review, previous findings on cognitive behavioral therapy (CBT) for gambling disorder are discussed as an established treatment available in Japan. Results of the review suggested that there are three CBT agendas for case formulation and intervention in gambling disorders. It would be important for future studies to (i) set intervention goals by focusing on improving adaptation, (ii) develop subtypes of gambling disorder based on descriptions of the function of gambling behaviors, and (iii) develop intervention techniques to modify the influence of risk situations. It is expected that findings of health psychology, which emphasizes the importance of adaptive behaviors would contribute to future CBT research and practice for gambling disorders. [ABSTRACT FROM AUTHOR]

Case: We present a case of brachytelephalangic chondrodysplasia punctata with a severe atlantoaxial dislocation in an underdeveloped child. The patient underwent halo jacket application using 10 halo pins with <1 lb/in² of torque, followed by posterior occipitocervical fusion with onlay rib and iliac autografts. After bone grafts and replacement of the halo ring multiple times, successful osseous fusion had been achieved by the 2-year follow-up. Conclusion: Although simple bone-grafting with a halo jacket is useful in underdeveloped patients with skeletal dysplasia, the complications related to halo fixation, including cranial bone perforation, and the patient's neurological status must be carefully monitored. [ABSTRACT FROM AUTHOR]

Unique dynamics using inanimate molecular assemblies based on soft matter have drawn much attention for demonstrating far-from-equilibrium chemical systems. However, there are no soft matter systems that exhibit a possible pathway linking the self-propelled oil droplets to formation of giant vesicles stimulated by low pH. In this study, we conceived an experimental oil-in-water emulsion system in which flocculated particles composed of a imine-containing oil transformed to spherical oil droplets that self-propelled and, after coming to rest, formed membranous figures. Finally, these figures became giant vesicles. From NMR, pH curves, and surface tension measurements, we determined that this far-from-equilibrium phenomenon was due to the acidic hydrolysis of the oil, which produced a benzaldehyde derivative as an oil component and a primary amine as a surfactant precursor, and the dynamic behavior of the hydrolytic products in the emulsion system. These findings afforded us a potential linkage between mobile droplet-based protocells and vesicle-based protocells stimulated by low pH. [ABSTRACT FROM AUTHOR]

We demonstrate two types of inversion of a helical preference upon the 1:1 complexation of a dynamic figure eight molecule with a guest molecule through the controlled transmission of point chirality. We designed a series of macrocycles that prefer a nonplanar conformation with figure eight chirality. These macrocycles are composed of a chirality-transferring unit (terephthalamide) and a structure-modifying unit (two o-phenylene rings spaced with a varying number of triple bonds). The former unit provides a binding site for capturing a guest molecule through the formation of hydrogen bonds. The attachment of chiral auxiliaries to the former unit induces a helical preference for a particular sense through the intramolecular transmission of point chirality. For relatively small-sized macrocycles, the preferred sense was reversed upon complexation with an achiral guest. Contrary preferences before and after complexation were both seen for chiral auxiliaries associated with a figure eight host through two-way intramolecular transmission of the single chiral source. Alternatively, the helical preference induced in relatively large-sized macrocycles was reversed only when a figure eight host formed a 1:1 complex with a particular enantiomeric guest through the supramolecular transmission of point chirality in the guest. This stereospecific inversion of a helical preference is rare. [ABSTRACT FROM AUTHOR]

The cardiac crescent is the first structure of the heart and contains progenitor cells of the first heart field, which primarily differentiate into left ventricular cardiomyocytes. The interface between the forming cardiac crescent and extraembryonic tissue is known as the juxta-cardiac field (JCF), and progenitor cells in this heart field contribute to the myocardium of the left ventricle and atrioventricular canal as well as the epicardium. However, it is unclear whether there are progenitor cells that differentiate specifically into left ventricular cardiomyocytes. We have previously demonstrated that an enhancer of the gene encoding the Hey2 bHLH transcriptional repressor is activated in the ventricular myocardium during mouse embryonic development. In this study, we aimed to investigate the characteristics of cardiomyocyte progenitor cells and their cell lineages by analyzing Hey2 enhancer activity at the earliest stages of heart formation. We found that the Hey2 enhancer initiated its activity prior to cardiomyocyte differentiation within the JCF. Hey2 enhancer-active cells were present rostrally to the Tbx5-expressing region at the early phase of cardiac crescent formation and differentiated exclusively into left ventricular cardiomyocytes in a lineage distinct from the Tbx5-positive lineage. By the late phase of cardiac crescent formation, Hey2 enhancer activity became significantly overlapped with Tbx5 expression in cells that contribute to the left ventricular myocardium. Our study reveals that a population of unipotent progenitor cells for left ventricular cardiomyocytes emerge in the JCF, providing further insight into the mode of cell type diversification during early cardiac development. [ABSTRACT FROM AUTHOR]

Ascomycete lectins may play an important role in their life cycle. In this report, we mined a ricin B-type lectin, named CmRlec, from the Cordyceps militaris genome by homology search. Furthermore, we succeeded in the soluble expression of CmRlec using β-glucuronidase as a solubilization tag and demonstrated that this lectin is a novel chitin-recognizing lectin. [ABSTRACT FROM AUTHOR]

A foldable cyclic oligomer 1 consisting of three terephthalamide units spaced with a 3-fold o-phenylene unit presented a dynamic pair of enantiomeric forms through molecular folding, to which the external chirality on a ditopic guest [(S,S)-2 or (R,R)-2] was supramolecularly transferred to prefer a particular sense of dynamic helicity [(M,M)-/(P,P)-1 and (M,M,P)-/(P,P,M)-1]. In the macrocycle, the terephthalamide units acted as exotopic binding sites to fold into helical forms upon complexation. The internal chirality associated with a host [(R,R,R,R,R,R)-1b] had no preference in a helical sense in the absence of a guest. Instead, the internal chirality was responsible for the signal modulation that it was cooperatively or competitively transferred in response to the external chirality on a guest (S,S)-2 or (R,R)-2. During the diastereomeric complexation, a particular sense of dynamic helicity was favored due to cooperative transmission of chirality when the helical preference was matched between the host and guest. Alternatively, the host complexed with an antipodal guest underwent a drastic change in conformation upon a change in temperature. [ABSTRACT FROM AUTHOR]

Although fluorescent, column-like supramolecular network structures were previously obtained by using 2-naphthalenecarboxylic acid and benzylamine or fluorobenzylamine, the use of chlorobenzylamine or bromobenzylamine results in column-like or 2D-layered network supramolecular structures, indicating that crystal structures can be controlled by altering the type and position of the substituent on the benzylamine component. [ABSTRACT FROM AUTHOR]

A noninvasive glucose monitoring system based on mid-infrared, attenuated total reflection spectros-copy using a hollow optical fiber probe is developed. Owing to the flexible fiber probe, measurement of oral mucosa, where blood capillaries are near the skin surface, is possible. Blood glucose levels are measured by detecting the peak intensity of glucose absorption bands, and the experimental results showed that the repro-ducibility of the measurement is high enough for monitoring blood glucose. [ABSTRACT FROM AUTHOR]

The reaction pathway of Cp*RuCl(cod)-catalyzed cyclization of allenynes was dramatically changed depending on the type of solvent employed. That is, while a cyclodimerization of allenyne proceeded to give a pentacyclic compound in toluene, a bicyclo[4.2.0]octa-1(8),5-diene derivative was exclusively produced by [2 + 2] cycloaddition of allenyne in methanol. It was also demonstrated that the [2 + 2] cycloaddition was catalyzed by a cationic ruthenium complex generated from Cp*RuCl(cod) and methanol in situ. [ABSTRACT FROM AUTHOR]

The cyclodimerization of α,ω-allenynes using Cp*RuCl(cod) catalyst is described. This reaction proceeds via an alkylidene ruthenacyclopentene intermediate, which is generated by the oxidative cycloaddition of alkyne and the internal double bond of the allene, to give a unique pentacyclic compound having two five-membered rings and three four-membered rings in high yields. [ABSTRACT FROM AUTHOR]

Pure metal nanowires (NWs) are one-dimensional nanomaterials with distinctive properties for various applications. Nevertheless, mass-growth forests have not been developed because of vapor pressure limitations, chemical reduction problems, or both. We succeeded in the mass growth of aluminum (Al) NW forests at desired locations by controlling atomic diffusion within the solid film. Whereas prior attention has focused only on how to increase the driving force, we show that focused ion beam irradiation created localized regions of high stress, which provided pathways for atomic diffusion as well as nuclei and driving forces for vertical NW growth. The underlying growth process could in principle be extended to other metals. [ABSTRACT FROM AUTHOR]

Acyl-CoAdiacylglycerol acyltransferase 1 (DGAT1) is a key enzyme in the fat absorption step in enterocytes. We previously reported that the pharmacological inhibition of DGAT1 increased plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activity in corn oil-loaded rats without any sign of hepatotoxicity. In this study, we investigated this mechanism. We found that this elevation occurred only during the pharmacologically active period of a DGAT1 inhibitor and the magnitude did not depend on the volume of corn oil. In addition, this elevation was not accompanied by increases in ALT or AST mRNA levels in the small intestine and liver. To clarify a lipid component responsible for this elevation, rats were treated with free fatty acids instead of corn oil and no plasma ALT elevation was observed. Next, rats were pretreated with inhibitors of monoacylglycerol acyltransferase 2 and intestinal microsomal triglyceride transfer protein instead of the DGAT1 inhibitor, but no plasma ALT elevation was observed after corn oil loading. Since the results suggested a possible role of diacylglycerol (DAG), which activates protein kinase C (PKC), we measured PKC activity in the small intestine and found that the activity was increased by treatment with the DGAT1 inhibitor and corn oil. Moreover, rats pretreated with a PKC inhibitor in combination with the DGAT1 inhibitor showed suppression of plasma ALT elevation. Taken together, the present results suggest that DAG accumulation induced by pharmacological DGAT1 inhibition and resultant PKC activation in enterocytes are involved in the increase in plasma ALT and AST activity in rats. [ABSTRACT FROM AUTHOR]

The interaction of dense plasmas with an intense laser under a strong external magnetic field has been investigated. When the cyclotron frequency for the ambient magnetic field is higher than the laser frequency, the laser's electromagnetic field is converted to the whistler mode that propagates along the field line. Because of the nature of the whistler wave, the laser light penetrates into dense plasmas with no cutoff density, and produces superthermal electrons through cyclotron resonance. It is found that the cyclotron resonance absorption occurs effectively under the broadened conditions, or a wider range of the external field, which is caused by the presence of relativistic electrons accelerated by the laser field. The upper limit of the ambient field for the resonance increases in proportion to the square root of the relativistic laser intensity. The propagation of a large-amplitude whistler wave could raise the possibility for plasma heating and particle acceleration deep inside dense plasmas. [ABSTRACT FROM AUTHOR]

Research trends on eating behaviors in young, healthy, girls in Japan were reviewed, and key perspectives and well-designed analog research aimed at developing clinical techniques for preventing eating disorders were examined. Many studies conducted in Japan have examined problems related to eating behaviors in young girls who are considered to be at high risk. However, conventional studies have not sufficiently contributed to improving the treatment of eating disorders. This could be because problems related to eating behavior are diverse, and studies that are unrelated to eating disorders have been reviewed. Moreover, treatment targets investigated to date have been inappropriate, and research methods have been limited. It is suggested that future studies should clarify the causal relationships of factors causing eating disorders through experimental methods. Furthermore, consideration of the quality of life, which is important in the treatment of eating disorders, should be considered. [ABSTRACT FROM AUTHOR]

Development and characterization of animal models of depression are essential for fully understanding the pathogenesis of depression in humans. We made and analyzed a mouse model exhibiting social deficit and hyperphagia-like behavior using a subchronic and mild social defeat stress (sCSDS) paradigm. The body weight, food and water intake of mice were monitored during a test period, and their behaviors and serum components were analyzed at two stages: immediately after the sCSDS period and 1 month after the sCSDS. The body weight and food intake of defeated mice were significantly higher than control mice at the sCSDS period, and these differences were sustained until 1 month after the sCSDS, whereas the water intake of defeated mice was significantly higher than control mice for the period of sCSDS only. Behavioral analyses revealed that the defeated mice exhibit significant social aversion to unfamiliar mice in a social interaction test and a trend of anxiety-like behavior in an elevated-plus maze test. Possibly due to polydipsia-like symptoms, defeated mice had significantly lower levels of albumin and blood urea nitrogen than control mice immediately after the sCSDS period but not at 1 month after sCSDS. The present study revealed that our sCSDS mice keep much more water in their body than control mice. This study reports the first step toward an understanding of the mechanisms of stress-induced overhydration, over-eating and resultant weight gain. [ABSTRACT FROM AUTHOR]

Nucleic acid oligonucleotides are widely used in hybridization experiments for specific detection of complementary nucleic acid sequences. For design and application of oligonucleotides, an understanding of their thermodynamic properties is essential. Recently, exciton-controlled hybridization-sensitive fluorescent oligonucleotides (ECHOs) were developed as uniquely labeled DNA oligomers containing commonly one thymidine having two covalently linked thiazole orange dye moieties. The fluorescent signal of an ECHO is strictly hybridization-controlled, where the dye moieties have to intercalate into double-stranded DNA for signal generation. Here we analyzed the hybridization thermodynamics of ECHO/DNA duplexes, and thermodynamic parameters were obtained from melting curves of 64 ECHO/DNA duplexes measured by ultraviolet absorbance and fluorescence. Both methods demonstrated a substantial increase in duplex stability (ΔΔG°37 ~ −2.6 ± 0.7 kcal mol-1) compared to that of DNA/DNA duplexes of the same sequence. With the exception of T·G mismatches, this increased stability was mostly unaffected by other mismatches in the position opposite the labeled nucleotide. A nearest neighbor model was constructed for predicting thermodynamic parameters for duplex stability. Evaluation of the nearest neighbor parameters by cross validation tests showed higher predictive reliability for the fluorescence-based than the absorbance-based parameters. Using our experimental data, a tool for predicting the thermodynamics of formation of ECHO/DNA duplexes was developed that is freely available at http://genome.gsc.riken.jp/echo/thermodynamics/. It provides reliable thermodynamic data for using the unique features of ECHOs in fluorescence-based experiments. [ABSTRACT FROM AUTHOR]

Peroxisome proliferator-activated receptors (PPARs) are a nuclear receptor family of ligand-inducible transcription factors, which have three different isoforms: PPAR&agr;, &dgr; and &ggr;. It has been demonstrated that PPAR&agr; and &ggr; agonists have renoprotective effects in proteinuric kidney diseases; however, the role of PPAR&dgr; agonists in kidney diseases remains unclear. Thus, we examined the renoprotective effect of GW501516, a PPAR&dgr; agonist, in a protein-overload mouse nephropathy model and identified its molecular mechanism. Mice fed with a control diet or GW501516-containing diet were intraperitoneally injected with free fatty acid (FFA)-bound albumin or PBS(2). In the control group, protein overload caused tubular damages, macrophage infiltration and increased mRNA expression of MCP-1 and TNF&agr;. These effects were prevented by GW501516 treatment. In proteinuric kidney diseases, excess exposure of proximal tubular cells to albumin, FFA bound to albumin or cytokines such as TNF&agr; is detrimental. In vitro studies using cultured proximal tubular cells showed that GW501516 attenuated both TNF&agr;- and FFA (palmitate)-induced, but not albumin-induced, MCP-1 expression via direct inhibition of the TGF-&bgr; activated kinase 1 (TAK1)-NFkB pathway, a common downstream signaling pathway to TNF&agr; receptor and toll-like receptor-4. In conclusion, we demonstrate that GW501516 has an anti-inflammatory effect in renal tubular cells and may serve as a therapeutic candidate to attenuate tubulointerstitial lesions in proteinuric kidney diseases. [ABSTRACT FROM AUTHOR]

Toll-like receptors (TLRs) are pattern recognition receptors that play a critical role in innate immune diseases. TLR3, which is localized in the endosomal compartments of hematopoietic immune cells, is able to recognize double-stranded RNA (dsRNA) derived from viruses and bacteria and thereby induce innate immune responses. Inflammatory periodontal bone resorption is caused by bacterial infections, which initially is regulated by innate immunity; however, the roles of TLR3 signaling in bone resorption are still not known. We examined the roles of TLR3 signaling in bone resorption using poly(I:C), a synthetic dsRNA analog. In cocultures of mouse bone marrow cells and stromal osteoblasts, poly(I:C) clearly induced osteoclast differentiation. In osteoblasts, poly(I:C) increased PGE2 production and upregulated the mRNA expression of PGE2-related genes, Ptgs2 and Ptges, as well as that of a gene related to osteoclast differentiation, Tnfsf11. In addition, we found that indomethacin (a COX-2 inhibitor) or an antagonist of the PGE2 receptor EP4 attenuated the poly(I:C)-induced PGE2 production and subsequent Tnfsf11 expression. Poly(I:C) also prolonged the survival of the mature osteoclasts associated with the increased mRNA expression of osteoclast marker genes, Nfatc1 and Ctsk. In ex vivo organ cultures of periodontal alveolar bone, poly(I:C) induced bone-resorbing activity in a dose-dependent manner, which was attenuated by the simultaneous administration of either indomethacin or an EP4 antagonist. These data suggest that TLR3 signaling in osteoblasts controls PGE2 production and induces the subsequent differentiation and survival of mature osteoclasts. Endogenous TLR3 in stromal osteoblasts and osteoclasts synergistically induces inflammatory alveolar bone resorption in periodontitis. [ABSTRACT FROM AUTHOR]

The article focuses on a study which examined the modification of thiol group(s) of cellular proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) in order to identify the molecular target of diallyl trisulfide (DATS) in human leukemic cell line U937. Topics covered include the effect of DATS on the growth of human monocytic leukemia U937 cells, and the protein(s) involved in the formation of either intra- or intermolecular disulfide bond.

Objective: To explore the relevance of citrullinated proteins and anti-citrullinated protein antibodies (ACPA) via protein arginine deiminase (PAD) inhibition in peptide glucose-6-phosphate isomeraseinduced arthritis (pGIA). Methods: Cl-amidine, a PAD inhibitor, was injected into pGIA. Clinical scores and histopathological findings of ankle joints were assessed. Serum ACPA titers were analyzed using ELISA. Citrullinated protein expression in joints and sera were examined with immunohistochemistry and Western blot analysis, respectively. Serum levels of IL-6, TNFa, and IL-1b were measured with cytometric bead array (CBA). Gene expression levels of IL-6 and TNFa in joints, lymph nodes, and spleens were analyzed with quantitative PCR. GPI-specific productions of IFNc and IL-17 from T cells in lymph nodes were evaluated. Results: Cl-amidine treatment significantly reduced arthritis severity while ACPA titers tended to be lower, but not significantly different compared to the control. Citrullinated proteins in joints and sera from treated mice were clearly decreased. With Cl-amidine treatment, serum IL-6 levels were significantly decreased, and IL-6 and TNFa gene expression were significantly reduced in joints. IL-17 production from GPI-specific T cells tended to be lower in Cl-amidine-treated mice, but not significantly different. Conclusion: Our results suggested that PAD-mediated citrullinated protein was involved in the pathogenesis of arthritis via IL-6. [ABSTRACT FROM AUTHOR]

Bmi1 is a polycomb group protein and regulator that stabilizes the ubiquitination complex PRC1 in the nucleus with no evidently direct link to the NF-κB pathway. In this study, we report a novel function of Bmi1: its regulation of IkBa ubiquitination in the cytoplasm. A deficiency of Bmi1 inhibited NF-κB--mediated gene expression in vitro and a NF-κB--mediated mouse model of arthritis in vivo. Mechanistic analysis showed that Bmi1 associated with the SCF ubiquitination complex via its N terminus and with phosphorylation by an IKKα/β-dependent pathway, leading to the ubiquitination of IκBα. These effects on NF-κB--related inflammation suggest Bmi1 in the SCF complex is a potential therapeutic target for various diseases and disorders, including autoimmune diseases. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate and compare the bactericidal efficacy of2%o chlorhexidine surfactant solution + 70%o alcohol and 2% chlorhexidine surfactant solution + 0.5% chlorhexidine-alcohol, and standardize skin antisepsis for blood collection from donor dogs. One hundred and twenty skin swabs of the jugular regions of 20 dogs were evaluated. Swabs were distributed into six treatment(T) groups according to the disinfectant used and removal or retention of local hair: T1 involved neither antisepsisnorhair removal; T2 comprised 2% chlorhexidine + 0.5% chlorhexidine-alcohol without hair removal; T3 comprised 2% chlorhexidine + 70% alcohol without hair removal; T4comprised hair removal but no antisepsis;T5comprised 2% chlorhexidine + 0.5% chlorhexidine-alcohol with hair removal; and T6comprised 2% chlorhexidine + 70% alcohol with hair removal. Antiseptic agents were continuously applied in a single direction for a total of 3 min. Use of antiseptics was effective with or without hair removal, resulting in the absence of bacterial growth. Complete efficacy of the technique used in this study may have been due to the increased antiseptic application time. In conclusion, the antisepsis protocols tested in this study can be safely used for the collection of blood from dogs; although, removal of hair prior to antisepsis is still recommended. [ABSTRACT FROM AUTHOR]

Background: The global demand for affordable carbon has never been stronger, and there is an imperative in many industrial processes to use waste streams to make products. Gas-fermenting acetogens offer a potential solution and several commercial gas fermentation plants are currently under construction. As energy limits acetogen metabolism, supply of H2 should diminish substrate loss to CO2 and facilitate production of reduced and energy-intensive products. However, the effects of H2 supply on CO-grown acetogens have yet to be experimentally quantified under controlled growth conditions. Results: Here, we quantify the effects of H2 supplementation by comparing growth on CO, syngas, and a high-H2 CO gas mix using chemostat cultures of Clostridium autoethanogenum. Cultures were characterised at the molecular level using metabolomics, proteomics, gas analysis, and a genome-scale metabolic model. CO-limited chemostats operated at two steady-state biomass concentrations facilitated co-utilisation of CO and H2. We show that H2 supply strongly impacts carbon distribution with a fourfold reduction in substrate loss as CO2 (61% vs. 17%) and a proportional increase of flux to ethanol (15% vs. 61%). Notably, H2 supplementation lowers the molar acetate/ethanol ratio by fivefold. At the molecular level, quantitative proteome analysis showed no obvious changes leading to these metabolic rearrangements suggesting the involvement of post-translational regulation. Metabolic modelling showed that H2 availability provided reducing power via H2 oxidation and saved redox as cells reduced all the CO2 to format directly using H2 in the Wood–Ljungdahl pathway. Modelling further indicated that the methylene-THF reductase reaction was ferredoxin reducing under all conditions. In combination with proteomics, modelling also showed that ethanol was synthesised through the acetaldehyde:ferredoxin oxidoreductase (AOR) activity. Conclusions: Our quantitative molecular analysis revealed that H2 drives rearrangements at several layers of metabolism and provides novel links between carbon, energy, and redox metabolism advancing our understanding of energy conservation in acetogens. We conclude that H2 supply can substantially increase the efficiency of gas fermentation and thus the feed gas composition can be considered an important factor in developing gas fermentation-based bioprocesses. [ABSTRACT FROM AUTHOR]

RNA-binding motif 10 (Rbm10) is an RNA-binding protein that regulates alternative splicing, but its role in inflammation is not well defined. Here, we show that Rbm10 controls appropriate splicing of DNA (cytosine-5)-methyltransferase 3b (Dnmt3b), a DNA methyltransferase, to regulate the activity of NF-κB-responsive promoters and consequently inflammation development. Rbm10 deficiency suppressed NF-κB-mediated responses in vivo and in vitro. Mechanistic analysis showed that Rbm10 deficiency decreased promoter recruitment of NF-κB, with increased DNA methylation of the promoter regions in NF-κB-responsive genes. Consistently, Rbm10 deficiency increased the expression level of Dnmt3b2, which has enzyme activity, while it decreased the splicing isoform Dnmt3b3, which does not. These two isoforms associated with NF-κB efficiently, and overexpression of enzymatically active Dnmt3b2 suppressed the expression of NF-κB targets, indicating that Rbm10-mediated Dnmt3b2 regulation is important for the induction of NF-κB-mediated transcription. Therefore, Rbm10-dependent Dnmt3b regulation is a possible therapeutic target for various inflammatory diseases. [ABSTRACT FROM AUTHOR]

Impact of stress on diseases including gastrointestinal failure is well-known, but molecular mechanism is not understood. Here we show underlying molecular mechanism using EAE mice. Under stress conditions, EAE caused severe gastrointestinal failure with high-mortality. Mechanistically, autoreactive-pathogenic CD4+ T cells accumulated at specific vessels of boundary area of third-ventricle, thalamus, and dentate-gyrus to establish brain micro-inflammation via stressgateway reflex. Importantly, induction of brain micro-inflammation at specific vessels by cytokine injection was sufficient to establish fatal gastrointestinal failure. Resulting micro-inflammation activated new neural pathway including neurons in paraventricular-nucleus, dorsomedial-nucleus-ofhypothalamus, and also vagal neurons to cause fatal gastrointestinal failure. Suppression of the brain micro-inflammation or blockage of these neural pathways inhibited the gastrointestinal failure. These results demonstrate direct link between brain micro-inflammation and fatal gastrointestinal disease via establishment of a new neural pathway under stress. They further suggest that brain micro-inflammation around specific vessels could be switch to activate new neural pathway(s) to regulate organ homeostasis. [ABSTRACT FROM AUTHOR]

Cognitive models of social anxiety posit that there are several types of maladaptive beliefs responsible for persistent social anxiety. Although these beliefs are theoretically important, currently there is no validated measure of these beliefs in Japan. In the present study, we translated into Japanese a well-validated measure of these beliefs, the Self-Beliefs Related to Social Anxiety (SBSA) Scale. The psychometric properties of the scale were also examined in two nonclinical samples (ns=401 and 30). Using confirmatory factor analysis, the Japanese version of the SBSA was found to have a correlated three-factor structure that consisted of "conditional beliefs," "unconditional beliefs," and "high standard beliefs." In addition, the Japanese version of the SBSA and its subscales demonstrated good internal consistency reliability and test-retest reliability. The Japanese version of the SBSA also demonstrated good convergent and divergent validity. Future applications of the Japanese version of the SBSA are discussed. [ABSTRACT FROM AUTHOR]

Objectives: This study investigated the association between several biological markers and fluorine-18 fluorodeoxyglucose (FDG) uptake in patients with hepatocellular carcinoma. Methods: Forty-two patients with hepatocellular carcinoma who underwent FDG positron emission tomography were included in the study. Tumor sections were immunohistochemically stained for phosphorylated signal transducer and activator of transcription 3 (pSTAT3), hypoxia-inducible factor 1α (HIF1α), glucose transporter 1 (GLUT1), GLUT2, GLUT3, and GLUT4. Results: The high standardized uptake value (SUV) group showed larger tumor size, more frequent vascular invasion, and poorer differentiation compared with the low SUV group. The high SUV group also showed significantly higher immunohistochemical expression of pSTAT3, HIF1α, and GLUT1. The GLUT1 high-expression group showed higher α-fetoprotein (a tumor marker) and poorer differentiation than did the GLUT1 low-expression group. Conclusions: Our study indicates that FDG uptake is associated with the expression of pSTAT3, HIF1α, and GLUT1 in hepatocellular carcinoma. The expression of these proteins shows a correlation with poor differentiation and vascular invasion. [ABSTRACT FROM AUTHOR]

The sensitive detection and quantification of DNA targets in the food industry and in environmental and clinical settings are issues of utmost importance in ensuring contamination-free food, monitoring the environment, and battling disease. Selective probes coupled with powerful amplification techniques are therefore of major interest. In this study, we set out to create an integrated microchemical chip that benefits from microfluidic chip technology in terms of sensitivity and a strong detection methodology provided jointly by padlock probes and rolling circle amplification (RCA). Here, we have integrated padlock probes and RCA into a microchip. The chip uses solid phase capture in a microchannel to enable washing cycles and decrease analytical area, and employs on-bead RCA for single-molecule amplification and detection. We investigated the effects of reagent concentration and amount of padlock probes, and demonstrated the feasibility of detecting Salmonella. [ABSTRACT FROM AUTHOR]