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The modern study of the exponential sums is mainly about their analytic estimates as complex numbers, which is local. In this paper, we study one global property of the exponential sums by viewing them as algebraic integers. For a kind of generalized Kloosterman sums, we present their degrees as algebraic integers. [ABSTRACT FROM AUTHOR]

Regulating nuclear export precisely is essential for maintaining mRNA homeostasis and impacts tumor progression. However, the mechanisms governing nuclear mRNA export remain poorly elucidated. Herein, it is revealed that the enhanced hypoxic long no‐ncoding RNA (lncRNA prostate cancer associated transcript 6 (PCAT6) in breast cancer (BC) promotes the nuclear export of m6A‐modified mRNAs, bolstering breast cancer stem cells (BCSCs) stemness and doxorubicin resistance. Clinically, hypoxic PCAT6 correlates with malignant BC features and poor prognosis. Mechanically, PCAT6 functions as a scaffold between interferon‐stimulated gene 15 (ISG15) and heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNPA2B1), leading to ISGylation of hnRNPA2B1, thus protecting hnRNPA2B1 from ubiquitination‐mediated proteasomal degradation. Interestingly, as an m6A reader, hnRNPA2B1 selectively mediates m6A‐tagged mRNAs nuclear export via the Aly/REF export factor (ALYREF)/ nuclear RNA export factor 1 (NXF1) complex, which promotes stemness‐related genes expression. HnRNPA2B1 knockdown or mRNA export inhibition can result in the retention of nuclear m6A‐tagged mRNA associated with stemness maintenance, which suppresses BCSCs self‐renewal and effectively improves the efficacy of doxorubicin therapy. These findings demonstrate the pivotal role of m6A‐modified mRNA nuclear export in BC progression, highlighting that the inhibition of m6A‐tagged mRNA and its nuclear export is a potential therapeutic strategy for the amelioration of cancer chemotherapy. [ABSTRACT FROM AUTHOR]

Isthmin‐1 (Ism1) plays roles in glucose uptake in mammals as an adipokine. To investigate its role in the glucose metabolism of common carp (Cyprinus carpio. L), the Ism1 sequence was cloned, and its expression and distribution in tissues were detected. In addition, we prepared and purified the recombinant Ism1 protein using the E. coli expression system and assessed changes in the expression of key genes related to glucose metabolism through both in vivo injection experiments and primary hepatocyte experiments in vitro. The results revealed that the open reading frame of Ism1 was 1377 bp long, encoding 458 amino acids. Similarity analysis indicated that Ism1 exhibited a close evolutionary relationship with goldfish (Carassius auratus), sharing 98.35% amino acid similarity. Ism1 was expressed in all tissues of common carp, with the highest level observed in the heart, followed by the gill, head kidney, and hepatopancreas. Distinct patterns of Ism1 expression were identified during the oral glucose tolerance test and long‐term high‐carbohydrate and high‐fat diet feeding experiments. In vivo studies demonstrated that the serum glucose concentration was reduced on treatment with Ism1, accompanied by a significant upregulation of mRNA levels for gk, hk, and pfk genes in hepatopancreas; conversely pepck and g6pase mRNA levels were significantly downregulated in the hepatopancreas under these conditions as well. Furthermore, our primary hepatocyte experiment confirmed that Ism1 could inhibit pepck and g6pase mRNA expression, while promoting gk, hk, and pfk mRNA expression levels. In conclusion, Ism1, in common carp, could participate in the glucose metabolism, which provides essential information for future studies on the function of Ism1. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of puerarin on myocardial inflammation in rats with heart failure from the Tolllike receptor 4 (TLR4)/nuclear transcription factor-κB (NF-κB) inflammatory pathway. Methods: The rat model of heart failure was established by subcutaneous injection of isoproterenol (5 mg/kg for 7 days), and was divided into model group, puerarin group, TLR4 activator group (RS09, 25 µg/kg), TLR4 inhibitor group (TAK-242, 0.5 mg/kg), puerarin+TLR4 activator group, with 20 rats in each group. Another 20 rats were selected as normal control group. Echocardiography was used to detect heart function; HE staining was used to observe pathological damage of myocardial tissue; TUNEL method was used to detect apoptosis of myocardial tissue cells; ELISA method was used to detect the levels of inflammatory factors IL-6, IL-1β and tumor necrosis factor-α (TNF-α) in myocardial tissue; immunohistochemical method was used to detect the expression of TLR4 and macrophage activation marker-F4/80; Western blot was used to detect the protein expressions of TLR4/NF-κB pathway, MyD88, IL-1 receptor-associated kinase (IRAK) and Galectin-3. Results: Compared with the normal control group, the necrosis, inflammatory infiltration and apoptosis of rat myocardial cells were severe in the model group, the heart function was decreased, the inflammatory factor release, macrophage activation, and TLR4/ NF-κB pathway protein expression were increased (P<0.05). Puerarin and TLR4 inhibitors could improve the pathological damage of heart failure rats such as myocardial cell necrosis, inflammatory infiltration and apoptosis to the same extent, improve heart function, reduce inflammatory response, inhibit macrophage activation and reduce TLR4/NF-κB pathway activation (P<0.05). TLR4 activator could promote the activation of TLR4/NF-κB pathway, aggravate myocardial cell damage, apoptosis and cardiac function damage in rats with heart failure, and weaken the above-mentioned effects of puerarin (P<0.05). Conclusion: Puerarin may inhibit the activation of TLR4/NF-κB pathway, reduce myocardial inflammation, and play an anti-heart failure effect. [ABSTRACT FROM AUTHOR]

AbstractThe experience of shidu (i.e., losing one’s only child) completely changes the lives and social interaction patterns of people. However, current research findings on interpersonal interactions of individuals who are experiencing shidu (referred to as “shiduers”) are inconsistent, reducing the effectiveness of support programs. Therefore, a qualitative analysis was conducted on the interpersonal interaction process of shiduers using the classic grounded theory. The results showed that “shidu” as an interpersonal information has two contradictory attributes, “family shame” and “family routine,” which create a social disabling dilemma for shiduers. Shiduers can reset their interpersonal relationships by establishing a clear inner and outer circle of relationships (centralizing) and by sequencing and typing relationships (differentiating). The interpersonal interactions of shiduers are complex and depend on the type of relationship. The current results can help community workers target individual support, family empowerment, and community building for people experiencing shidu. [ABSTRACT FROM AUTHOR]

The single cleavage drilled compression specimens of sandstone were impacted by the large-diameter split Hopkinson pressure bar, during which the whole model-I dynamical fracture process was successfully observed. A crack propagation gauge is used to monitor the key time moment of dynamic initiation, propagation, arrest and re-initiation, respectively. The fractal crack extension model is used to analysis the propagation speed of the tortuous crack, and with further combination of the experimental–numerical-analytical method, to determine the dynamic initiation toughness, dynamic propagation toughness, dynamic arrest toughness, and dynamic re-initiation toughness of sandstone. The results show that in the process of crack propagation, the crack propagation path is torturous; and for this curved path, the value of the universal function, which is characterized by the crack's velocity, is smaller than that with a straight path. The dynamic propagation toughness thus obtained is closer to its real value by using the fractal model. Sandstone's dynamic initiation toughness is greater than the dynamic arrest toughness, and the dynamic initiation toughness is slightly bigger than the dynamic re-initiation toughness. [ABSTRACT FROM AUTHOR]

In this paper, we study the Newton polygon of the L -function of a generalized Kloosterman polynomial with two variables over finite fields. We give the explicit form of the monomial basis of the top dimensional cohomology space of the p -adic complex associated to the L -function. One consequence of this result is a concrete method for computing the Hodge polygon. Using decomposition theorems of Wan, we determine when a generalized Kloosterman polynomial is ordinary and when its Newton polytope is generically ordinary. [ABSTRACT FROM AUTHOR]

Purpose: Heart failure (HF) remains a major cause of late morbidity and mortality after myocardial infarction (MI). To date, no clinically established 18F-labeled sympathetic nerve PET tracers for monitoring myocardial infarction are available. Therefore, in this study, we synthesized a series of 18F-labeled benzyl guanidine analogs and evaluated their efficacy as cardiac neuronal norepinephrine transporter (NET) tracers for myocardial imaging. We also investigated the preliminary diagnostic capabilities of these tracers in myocardial infarction animal models, as well as the structure-activity relationship of these tracers. Procedures: Three benzyl guanidine-NET tracers, including [18F]1, [18F]2, and [18F]3, were synthesized and evaluated in vivo as PET tracers in a myocardial infarction mouse model. [18F]LMI1195 was used as a positive control for the tracers. H&E staining of the isolated myocardial infarction heart tissue sections was performed to verify the efficacy of the selected PET tracer. Results: Our data show that [18F]3 had a moderate decay corrected labeling yield (~10%) and high radiochemical purity (>95%) compared to other tracers. The uptake of [18F]3 in normal mouse hearts was 1.7±0.1%ID/cc at 1 h post-injection (p. i.), while it was 2.4±0.1, 2.6±0.9, and 2.1±0.4%ID/cc in the MI mouse hearts at 1, 2, and 3 days after surgery, respectively. Compared with [18F]LMI1195, [18F]3 had a better myocardial imaging effect in terms of the contrast between normal and MI hearts. The area of myocardial infarction shown by PET imaging corresponded well with the infarcted tissue demonstrated by H&E staining. Conclusions: With an obvious cardiac uptake contrast between normal mice and the myocardial infarction mouse model, [18F]3 appears to be a potential tool in the diagnosis of myocardial infarction. Therefore, it is necessary to conduct further structural modification studies on the chemical structure of [18F]3 to improve its in vivo stability and diagnostic detection ability to achieve reliable and practical imaging effects. [ABSTRACT FROM AUTHOR]

Simple Summary: Rhizosphere microbial composition was affected by plant cultivars, soil properties, climate and other conditions. However, the effects of cultivars on the structure and the function of rhizosphere microbial communities have not been reported completely. In this study, the rhizosphere bacterial community composition of eight cultivars of Paeonia lactiflora was revealed by high-throughput sequencing technology. The differences and similarities of rhizosphere bacterial community composition and rhizosphere bacterial interaction among different cultivars were analyzed. In addition, cultivation-related microbial resources that can be used for bioremediation, organic degradation and disease resistance have been identified. This research will contribute to the development of rhizosphere microbial resources of P. lactiflora and provide guidance for agricultural practice in the future. The composition and diversity of the rhizosphere microbial community maintain the stability of the root microclimate, and several studies have focused on this aspect of rhizosphere microorganisms. However, how these communities vary with cultivars of a species is not completely understood. Paeonia lactiflora—a perennial herb species of the family Paeoniaceae—includes a wide variety of cultivars, with rich rhizosphere microbial resources. Hence, we studied the differences in rhizosphere bacterial communities associated with eight P. lactiflora cultivars. We noted that Actinobacteria, Proteobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia, Planctomycetes and Chloroflexi were the dominant phyla associated with the cultivars. The composition of rhizosphere bacterial community of different cultivars was highly similar at taxonomic levels, but there were slightly differences in the relative abundance. LEfSe analysis showed that the cultivars "Sheng Tao Hua" and "Zi Lou Xian Jin" exhibited the most biomarkers. Differential ASV analysis revealed the maximum difference in ASV abundance between "Lian Tai" and "Zi Hong Zheng Hui", as well as between "Sheng Tao Hua" and "Tao Hua Fei Xue", and the maximum similarity between "Duo Ye Zi" and "Xue Feng". Co-occurrence network analysis revealed that rhizosphere bacteria in most cultivars maintain homeostasis by cooperation, wherein Actinobacteria and Proteobacteria played a vital role. In addition, microbial resources related to cultivars like bioremediation, organic degradation and resistance to diseases are found. This study revealed the structures of the rhizosphere bacterial communities associated with different cultivars of P. lactiflora and explored their stress resistance potential, which can be used to guide future agricultural practices. [ABSTRACT FROM AUTHOR]

Purpose: The radiopharmaceutical [18F]AlF-NOTA-FAPI-04 presents a promising alternative to 68 Ga-FAPI owing to its relatively longer half-life. This study aimed to evaluate the clinical usefulness of [18F]AlF-NOTA-FAPI-04 PET/CT for the diagnosis of primary and metastatic lesions in various types of gastrointestinal system cancers, compared with 18F-FDG PET/CT. Methods: Patients diagnosed with gastrointestinal system malignancies were prospectively enrolled. All patients underwent both 18F-FDG and 18F-FAPI-04 PET/CT scans within one week, with 44 (73.3%) for cancer staging and 16 (26.7%) for tumor restaging. Diagnostic efficacy of the primary tumor, as well as the presence and number of lymph nodes and distant metastases, were assessed. Tumor uptake was quantified by the maximum standard uptake value (SUVmax). Results: For detection of primary tumor, the diagnostic sensitivity of 18F-FDG PET/CT was 72.7%, while it was 97.7% for 18F-FAPI-04 PET/CT. Based on per-lymph node analysis, the sensitivity, specificity, and accuracy of 18F-FAPI-04 PET/CT in diagnosing metastatic lymph nodes were 91.89%, 92.00%, and 91.96%, respectively. These values were notably higher than those 18F-FDG PET/CT (79.72%, 81.33% and 80.80%, respectively). The 18F-FAPI-04 PET/CT surpassed 18F-FDG PET/CT in detecting suspected metastases in the brain (7 vs. 3), liver (39 vs. 20), bone (79 vs. 51), lung (11 vs. 4), and peritoneal carcinoma (48 vs. 22). Based on per-patient analysis, differential diagnostic accuracies (18F-FAPI-04 vs. 18F-FDG PET/CT) were observed in all patients (91.7% vs. 76.7%), the initial staging group (90.9% vs. 79.5%), and the re-staging group (93.8% vs. 68.7%). Additionally, 18F-FAPI-04 PET/CT revised final diagnosis in 31.7% of patients, contrasting with 18F-FDG PET/CT, and prompted changes in clinical management for 21.7% of the patients. Conclusion: 18F-FAPI-04 PET/CT outperforms 18F-FDG PET/CT in delineating the primary gastrointestinal tumors and detecting suspected metastatic lesions due to a higher target-to-background ratio (TBR). Moreover, 18F-FAPI-04 PET/CT could provide valuable guidance for tumor staging, thereby having a potential impact on patient management. [ABSTRACT FROM AUTHOR]

Background: Epidemiological studies have demonstrated an association between red blood cell distribution width (RDW) and the prognosis of pneumonia-associated diseases. However, prognostic value of RDW in patients with ventilator-associated pneumonia (VAP) has yet to be investigated. This study aimed to explore the association between RDW and in-hospital mortality in VAP patients and explore predictive value of RDW for VAP patients. Methods: This retrospective cohort study included 1,543 VAP patients from the Medical Information Mart for Intensive Care IV database 2008-2019. The primary outcome was considered to 30-day in-hospital mortality of VAP patients in this study. Non-high RDW level group was defined as <15 %, and high RDW level group as ≥15%. The possible confounding factors were screened by least absolute shrinkage and selection operator regression. Univariate and multivariate COX regression analyses were used for the assessment on the association of RDW and 30-day in-hospital mortality in VAP patients. We also performed subgroup analyses. Furthermore, a comparative analysis of RDW and sequential organ failure assessment (SOFA) score and simplified acute physiology score II (SAPS II) were performed by receiver operating characteristic (ROC) curves. Results: The 30-day in-hospital mortality of VAP patients was approximately 19.05%. After adjusting all confounding factors, high RDW was associated with 30-day in-hospital mortality among VAP patients by using non-high RDW as the reference [hazard ratio (HR) =1.29, 95% confidence interval (CI): 1.01-1.63]. Additionally, the relationship was also robust in several populations, such as patients were younger than 60 years, or had not a history of congestive heart failure, or had a history of sepsis, or had not received renal replacement therapy, or had a duration of mechanical ventilation for more than 7 days. The result of ROC indicated that RDW had a better prognostic value in predicting 30-day in-hospital mortality for VAP patients than SOFA score and SAPS II score. Conclusion: High RDW level is associated with an increased 30-day in-hospital mortality. The RDW is a promising biomarker in predicting 30-day in-hospital mortality for patients admitted to the ICU, regardless of VAP. [ABSTRACT FROM AUTHOR]

Rapid and accurate methods for the diagnosis of tuberculous pleurisy (TP) are urgently needed. Activation markers of tuberculosis (TB)–reactive T cells are considered promising for the diagnosis of active TB (ATB). Different activation indexes may play different roles in the progression of TB, but there are few reports on T cell activation indicators, except for HLA-DR. Hence, we evaluated the expression of early (CD25 and CD69) and late (CD134) activation markers on TB antigen-stimulated CD4+ T cells in populations with different TB infection status and investigated their diagnostic value for ATB, particularly, for TP. Moreover, we compared the differences in the diagnostic efficacy among the indexes from peripheral blood (PB) and pleural fluid (PF) for TP. The expression of each activation marker was significantly increased in TB-infected populations (patients with ATB and latent TB infection vs. healthy individuals; patients with TP vs. non-TP) and was significantly higher in the PF than in the PB of patients with TP. The diagnostic performance of the coexpressed activation markers was superior to that of single expression markers in the differential diagnosis of ATB and non-TB, with CD25+CD134+ showing the best diagnostic efficiency (AUC: 0.93, 95% CI, 0.87–0.99; sensitivity: 86.7%, 95% CI, 72.5%–94.5%; and specificity: 94.0%, 95% CI, 82.5%–98.4%). Except for TB-IGRA, the activation indexes were more accurate than conventional laboratory methods for ATB diagnosis. In addition, the expression of CD25+CD134+ in PB and PF was the best values for differential diagnosis of TP and NTP, with AUCs of 0.87 (95% CI, 0.77–0.96) and 0.95 (95% CI, 0.90–1.00), respectively. Our study provides information on the diagnostic value of different activation markers for TB and shows that the expression of CD25+CD134+ on CD4+ T cells in PF can serve as a potential marker for TP diagnosis. [ABSTRACT FROM AUTHOR]

Artificial intelligence (AI) and machine learning (ML) have been applied to solve various remote sensing problems. To fully leverage the power of AI and ML to tackle impactful remote sensing problems, it is essential to enable researchers and practitioners to understand how AI and ML models actually work and thus to improve the model performance strategically. Accurate and timely land cover maps are essential components for informed land management decision making. To address the ever-increasing need for high spatial and temporal resolution maps, this paper developed an interactive and open-source online tool, in Python, to help interpret and improve the ML models used for land cover mapping with Google Earth Engine (GEE). The tool integrates the workflow of both land cover classification and land cover change dynamics, which requires the generation of a time series of land cover maps. Three feature importance metrics are reported, including impurity-based, permutation-based, and SHAP (Shapley additive explanations) value-based feature importance. Two case studies are presented to showcase the tool's capability and ease of use, enabling a globally accessible and free convergent application of remote sensing technologies. This tool may inspire researchers to facilitate explainable AI (XAI)-empowered remote sensing applications with GEE. [ABSTRACT FROM AUTHOR]

MHD turbulence is considered to be anisotropic owing to the presence of a magnetic field, and its self-correlation anisotropy has been unveiled by solar wind observations. Here, based on numerical results of compressible MHD turbulence with a global mean magnetic field, we explore variations of the normalized self-correlation function's (NCF) level contours with the scale as well as their evolution. The analyses reveal that the NCF's level contours tend to elongate in the direction parallel to the mean magnetic field, and the elongation becomes weak with decreasing intervals. These results are consistent with slow solar wind observations. The less anisotropy of the NCF's level contours with the shorter intervals can be produced by the fact that coherent structures stretch more along the parallel direction at the long intervals than at the short intervals. The analyses also disclose that as the simulation time builds up, the NCF's level contours change thinner and thinner, and the anisotropy of the NCF's level contours grows, which can be caused by the break of large coherent structures into small ones. The increased self-correlation anisotropy with time foretells that the self-correlation anisotropy of solar wind turbulence enlarges with the radial distance, which needs to be tested against observations by using Parker Solar Probe (PSP) measurements. [ABSTRACT FROM AUTHOR]

People tend to perceive a virtual body standing in front of them as their own if it is either stroked or moving synchronously with their own real body—the out-of-body experience (OBE). We combined synchrony manipulation with two other factors of theoretical interest: the kind of stimulation, visuotactile stimuli or visuomotor correlations, being synchronised and the interoceptive accuracy (IA) of participants, assessed by means of the heartbeat counting task. Results showed that explicit measures of embodiment were systematically affected by synchrony, and this synchrony effect was more pronounced for visuomotor than for visuotactile conditions. The walking drift was affected by IA: In visuotactile conditions, the synchrony effect was pronounced in individuals with low IA, presumably reflecting a stronger impact of the visual information. In visuomotor conditions, however, the synchrony effect was stronger in individuals with high IA, presumably reflecting a stronger impact of re-afferent information generated by the participants' own movements. [ABSTRACT FROM AUTHOR]

Although magnetic reconnection plays a key role in the formation of a solar filament, the detailed formation process is still ambiguous. Combining the observational data from the New Vacuum Solar Telescope and the Solar Dynamics Observatory, we analyzed the formation of a U -shaped filament via successive magnetic reconnection in the AR NOAA 11598 on 2012 October 25. The successive reconnection occurred between a filament (F) and its nearby chromospheric fibrils (CF). The associated brightening and magnetic cancellation were observed. The changes in appearance of the CF at the reconnection site were accompanied by the formation and accumulation of some new magnetic loops, as well as plasmas propagated along the formed magnetic loops from the reconnection site, indicating the changes in the topology of the F and CF. These can provide comprehensive observational evidence for successive reconnection. After the reconnection, a longer U -shaped filament was formed. During the formation of the U -shaped filament, two major magnetic energy releases took place. While in the two energy release processes, the injected plasma from the reconnection site can provide part of the material for the formation of the U -shaped filament. Therefore, we conclude that the successive reconnection results in both the dynamical evolution and the subsequent formation associated with the U -shaped filament. And the results of nonlinear force-free field extrapolation demonstrated that the magnetic topology of the F was changed significantly; this is consistent with the observational results and further confirms the formation of the U -shaped filament. [ABSTRACT FROM AUTHOR]

Zika virus (ZIKV) is a mosquito-borne flavivirus and causes an infection associated with congenital Zika syndrome and Guillain–Barre syndrome. The mechanism of ZIKV-mediated neuropathogenesis is not well understood. In this study, we discovered that ZIKV induces degradation of the Numb protein, which plays a crucial role in neurogenesis by allowing asymmetric cell division during embryonic development. Our data show that ZIKV reduced the Numb protein level in a time- and dose-dependent manner. However, ZIKV infection appears to have minimal effect on the Numb transcript. Treatment of ZIKV-infected cells with a proteasome inhibitor restores the Numb protein level, which suggests the involvement of the ubiquitin–proteasome pathway. In addition, ZIKV infection shortens the half-life of the Numb protein. Among the ZIKV proteins, the capsid protein significantly reduces the Numb protein level. Immunoprecipitation of the Numb protein co-precipitates the capsid protein, indicating the interaction between these two proteins. These results provide insights into the ZIKV–cell interaction that might contribute to its impact on neurogenesis. [ABSTRACT FROM AUTHOR]

This study sought to examine the role of bile acids in the regulation of glucose and lipid metabolism, intestinal flora, and growth in high-fat diet-fed common carp (Cyprinus carpio L.). Fish (6.34 ± 0.07 g) were fed for 56 days with three different diets, the control diet (CO, 5.4% lipid), high-fat diet (HF, 11% lipid), and high-fat diet with 60 mg/kg bile acids (BAs, 11% lipid). The results showed that high-fat diets resulted in poor growth performance and increased triglyceride (TG) in serum and the liver. The addition of bile acids significantly alleviated the adverse effects of a high-fat diet. The mRNA expression results indicated that bile acids may improve lipid metabolism through the enhancement of the peroxisome proliferator-activated receptor (PPARa). The expression of gluconeogenesis-related phosphoenolpyruvate carboxykinase (PEPCK) mRNA was inhibited, while fibroblast growth factor 19 (FGF19) was significantly higher. Bile acids reshaped the intestinal microflora community, with the level of Bacteroidetes increasing. The correlation analysis indicated that Patescibacteria, Dependentiae, Myxococcota, and Planctomycetota in the gut are associated with genes involved in glucose and lipid metabolism. These results indicated that bile acids could ameliorate the negative effects of high-fat diets on common carp. [ABSTRACT FROM AUTHOR]

Guillain-Barré syndrome (GBS) is an acute idiopathic polyneuropathy which is related to infection and immune mechanism. The exact pathogenesis of the disease is unknown and treatment is limited. Thus, the purpose of the study is to identify biomarkers of GBS serum and elucidate their involvement in the underlying pathogenesis of GBS that could help to treat GBS more accurately. Antibody array technology was used to detect the expression levels of 440 proteins in serum of 5 GBS group and 5 healthy control group. Sixty-seven differentially expressed proteins (DEPs) were identified by antibody array, among which FoLR1, Legumain, ErbB4, IL-1α, MIP-1α and IGF-2 were down-regulated, while 61 proteins were up-regulated. Bioinformatics analysis indicated that most DEPs were associated with leukocytes, among which IL-1α, SDF-1b, B7-1, CD40, CTLA4, IL-9, MIP-1α and CD40L were in the center of protein–protein interaction (PPI) network. Subsequently, the ability of these DEPs to distinguish GBS from healthy control was further evaluated. CD23 was identified by means of Random Forests Analysis (RFA) and verified by enzyme-linked immunosorbent assay (ELISA). The ROC curve result of CD23 respectively displayed that its sensitivity, specificity and AUC were 0.818, 0.800 and 0.824. We speculate that activation of leukocyte proliferation and migration in circulating blood might be associated with inflammatory recruitment of peripheral nerves, leading to the occurrence and development of GBS, but this conclusion still requires deeper confirmation. More importantly, central proteins may play a pivotal role in the pathogenesis of GBS. In addition, we detected IL-1α, IL-9, and CD23 in the serum of GBS patients for the first time, which may be promising biomarkers for the treatment of GBS. [ABSTRACT FROM AUTHOR]

The burden of stomach cancer remains high in Hong Kong. We sought to evaluate the associations of age, period, and birth cohort with the changing trend in the incidence of stomach cancer and to provide projections through 2030. We performed an age-period-cohort analysis and projections up to 2030 using data from the Hong Kong Cancer Registry. Additionally, we used a population decomposition algorithm to assess the drivers in the number of incident cases of stomach cancer in Hong Kong. Among the 26,813 stomach cancer patients, from 1994 to 2018, the age-standardized incidence rate of stomach cancer decreased for both sexes. The incidence increased with age and was highest for those aged 85 years or older. Period relative risk (RR) showed a monotonic decreasing pattern throughout the study period for both sexes before 2010. Cohort RR for males was monotonically decreasing but changed little after the 1967–1971 birth cohort. In contrast, cohort RR for females declined in the pre-1927–1931 birth cohort but slowed down since. It is projected that there will be 906 male patients and 954 female patients in 2030. Decomposition analysis suggested that population growth and aging were associated with substantial changes in the number of incident cases of stomach cancer in Hong Kong. Both period and cohort risk of developing stomach cancer in Hong Kong have slowed down or plateaued. Our study demonstrates that population aging and growth are the main drivers of the increased number of incident cases of stomach cancer in Hong Kong. [ABSTRACT FROM AUTHOR]

A 56-day feeding trial was conducted to investigate the effects of genistein on growth, lipid metabolism, antioxidant capacity, and immunity of common carp fed with high-carbohydrate or high-fat diets. Five diets were used to feed fish: control diet (5% fat; CO), high-fat diet (11% fat; HF), high-carbohydrate diet (45% carbohydrate; HC), and HF or HC diet with 500 mg/kg genistein (FG or CG). Results showed that final body weight (FW) and specific growth rate (SGR) were significantly reduced, but the supplementation with genistein resulted in higher values of FW and SGR than the HF or HC group. Both high carbohydrate and high fat belong to high-energy diets, which may promote lipid deposition. Genistein obviously decreased liver triglyceride (TG) content and alleviated hepatic fat vacuolation in the HF and HC groups. The expression of lipid metabolism genes (cpt-1 and atgl) was markedly higher in the FG group than in the HF group. The lipid synthesis-related genes (fas, acc, and pparγ) were elevated in high-energy diets but recovered to the control level or reduced after genistein treatments. With respect to fatty acid transporter genes, fatp increased in the FG group, and cd36 increased in the CG group. Furthermore, the antioxidant and immune indexes, such as total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), acid phosphatase (ACP), and lysozyme (LZM) activities, were decreased, while malonate aldehyde (MDA) content, activities of alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were enhanced in the HF and HC groups. The antioxidant and immunity values could be ameliorated by treatment with genistein. Moreover, the transcript levels of antioxidant-related genes (cat, gr, and nrf2) in the liver and anti-inflammatory factors (tgf-β and il-10) and lyz in the head kidney tissue were promoted, although the expression levels of proinflammatory factors (tnf-α and il-6) declined in the genistein supplementation group, which confirmed the antioxidant and immune-enhancing effects of genistein. Therefore, 500 mg/kg genistein could ameliorate the negative effects of high-energy diets on immunity. [ABSTRACT FROM AUTHOR]

Background: This study investigated the effect of oral nutritional supplements (ONS) on patients with esophageal cancer during radiotherapy. Materials and Methods: Patients with esophageal cancer undergoing radiotherapy were randomly divided into nutritional intervention (NI) and routine treatment (RT) groups. All patients received one-on-one nutritional counseling and dietary advice, and patients in the NI group received ONS. Body mass index (BMI), a patient-generated subjective global assessment (PG-SGA), serum albumin (ALB), hemoglobin (Hb), white blood cell (WBC) count, prealbumin (PA), and platelets (PLTs) were recorded before and after radiotherapy in both the NI group and the RT group. Further, the adverse reactions during the treatment were evaluated. Results: In the NI group, the BMI after radiotherapy was significantly higher than that before radiotherapy, and the PG-SGA score after radiotherapy was significantly lower than that before radiotherapy (p < 0.05). In the RT group, the levels of BMI, Hb, ALB, PA, PLTs, and WBC after radiotherapy were significantly lower than those before radiotherapy, and the PG-SGA score was worse after radiotherapy (p < 0.05). After radiotherapy, the levels of BMI, Hb, ALB, and PA in the NI group were similar to those in the RT group (p > 0.05); whereas the levels of WBC and PLTs in the NI group were lower than those in the RT group (p < 0.05). During the treatment, the toxic reactions of radiation esophagitis, myelosuppression, nausea, and vomiting were decreased in the NI group compared with the RT group. Conclusions: ONS can reduce weight loss and improve the nutritional status of patients with esophageal cancer during radiotherapy. [ABSTRACT FROM AUTHOR]

This work illustrates the accelerated decline in illegal drug use during the COVID‐19 pandemic in China. We first reviewed the global effects of the COVID‐19 pandemic on the situation of illegal drugs. We then compared the data of the pre‐pandemic (2016–2019) and pandemic period (2020–2022) for drug seizures, individuals identified as using drugs, registered and newly discovered illegal drug users and the number of community‐treated drug users to demonstrate the fast decline in the use of illegal drugs in China. We then discussed the possible reasons and additional considerations for these changes. Overall, the COVID‐19 controls in China, such as all‐staff nucleic acid testing and ID‐based dynamic monitoring, substantially reduced illegal drug use. Being wary of a possible rebound in drug use and preventing new types of drug crimes are still essential in post‐COVID China. [ABSTRACT FROM AUTHOR]

This paper presents a study of a 2021 October 9 coronal mass ejection (CME) with multipoint imaging and in situ observations. We also simulate this CME from the Sun to Earth with a passive tracer to tag the CME's motion. The coronagraphic images show that the CME is observed as a full halo by SOHO and as a partial halo by STEREO-A. The heliospheric images reveal that the propagation speed of the CME approaches about 1° hr−1, suggesting a slow CME. With simulated results matching these observation results, the simulation discloses that as the CME ejects from the Sun out to interplanetary space, its global morphology is distorted from an ellipsoid to a concave shape owing to interactions with the bimodal solar wind. The cross section of the CME's flux rope structure transforms from a circular shape into a flat one. As a result of the deflection, the propagation direction of the CME is far away from the Sun–Earth line. This means that the CME flank (or the ICME leg) likely arrives at both Solar Orbiter and the L1 point. From the CME's eruption to 1 au, its volume and mass increase by about two orders and one order of magnitude, respectively. Its kinetic energy is about 100 times larger than its magnetic energy at 1 au. These results have important implications for our understanding of CMEs' morphology, as well as their space weather impacts. [ABSTRACT FROM AUTHOR]

Objectives: Kaempferol (KMF), has beneficial effects against hepatic lipid accumulation. In this study, we aimed to investigate molecular mechanism underlying the protective effect of KMF on lipid accumulation. Methods: HepG2 cells were treated with different concentrations of KMF and 0.5 mM palmitate (PA) for 24 h. The mRNA and protein levels of genes involved in lipid metabolism were evaluated using real-time PCR and western blot. The expression of Nrf2 was silenced using siRNA. Results: Data indicated that KMF (20 μM) reversed PA-induced increased triglyceride (TG) levels and total lipid content. These effects were accompanied by down-regulation of the mRNA and protein levels of lipogenic genes (FAS, ACC and SREBP1), and up-regulation of genes related to fatty acid oxidation (CPT-1, HADHα and PPARα). Kaempferol significantly decreased the levels of the oxidative stress markers (ROS and MDA) and enhanced the activities of antioxidant enzymes SOD and GPx in PA-challenged cells. Luciferase analysis showed that KMF increased the transactivation of Nrf2 in hepatocytes. The results also revealed that KMF-mediated activation of Nrf2 target genes was suppressed by Nrf2 siRNA. Furthermore, Nrf2 siRNA abolished the KMF-induced reduction in ROS and MDA levels in PA treated cells. In addition, the inhibitory effect of KMF on TG levels and the mRNA and protein levels of FAS, ACC and SREPB-1 were significantly abolished by Nrf2 inhibition. Nrf2 inhibition also suppressed the KMF-induced activation of genes involved in β oxidation (CPT-1 and PPAR-α). Conclusion: The results suggest that KMF protects HepG2 cells from PA-induced lipid accumulation via activation of the Nrf2 signaling pathway. [ABSTRACT FROM AUTHOR]

Polyvinyl chloride (PVC) foam sandwich panels have a wide application prospect in the field of warship impact protection due to the low moisture absorption, low density, excellent energy consumption and chemical resistance of PVC foam. The dynamic response of layered density-graded PVC foam sandwich panel under impact load was experimentally and numerically investigated here. A three-layer PVC foam sandwich specimen was designed and fabricated. The graded strategy of foam core was achieved by setting various foam layers with different densities. A large-diameter split-Hopkinson pressure bar device was used to perform the experimental study. The effect of density-graded strategy of foam core on the transmitted force, transmitted impulse and energy absorption were discussed. The software ANSYS/LS-DYNA was used to simulate the dynamic impact test of the foam sandwich structure. The simulation results are in good agreement with the experimental results. The results reveal that, the order of the core layer of density-graded sandwich panel has little effect on the impact resistance of the structure, while the impact resistance can be improved by increasing the density difference between foam layers. The uniform configuration UD has the largest impact force and the most specific energy absorption, while the large gradient configuration large gradient configuration has the smallest transmitted impulse under various impact velocities of the projectile. Thus, large gradient configuration has better protection effect. [ABSTRACT FROM AUTHOR]

In this paper, we present a hybrid algorithm based on parareal and Schwarz waveform relaxation (SWR) for solving time dependent partial differential equations. The parallelism can be simultaneously realized in the time direction by using a parareal and in the space direction via SWR. We give a convergence analysis for the hybrid algorithm for a 1D model problem, the reaction-diffusion equation. Weak scaling of the algorithm in terms of both the number of space subdomains and the number of paralleled time intervals were investigated via theoretical analysis and numerical experiments. [ABSTRACT FROM AUTHOR]

Existing assessment methods of writing originality have been criticized for depending heavily on subjective scoring methods. This study attempted to investigate the use of topic analysis and semantic networks in assessing writing originality. Written material was collected from a Chinese language test administered to eighth-grade students. Two steps were performed: 1. Latent topics of essays in each writing task were identified, and essays on the same topic were treated as a refined reference group, within which an essay was to be evaluated; 2. A group of features was developed, including four categories, i.e., path distance, semantic differences, centrality, and similarity of the network drawn from each text response, which were used to quantify the differences among essays. The results show that writing originality scoring is not only related to the intrinsic characteristics of the text, but is also affected by the reference group in which it is to be evaluated. This study proves that computational linguistic features can be a predictor of originality in Chinese writing. Each feature type of the four categories can predict originality, although the effect varies across various topics. Furthermore, the feature analysis provided evidence and insights to human raters for originality scoring. [ABSTRACT FROM AUTHOR]

Cancer poses a serious threat to human health and is the most common cause of human death. Polymer-based nanomedicines are presently used to enhance the treatment effectiveness and decrease the systemic toxicity of chemotherapeutic agents. However, the disadvantage of currently polymeric carriers is without therapy procedure. Herein, for the first time, glutathione (GSH)-responsive polymer (PRES) with anti-cancer effect was synthesized following the condensation-polymerization method using resveratrol (RES) and 3,30-dithiodipropionic acid. PRES can not only suppress the tumor cells growth but can also self-assemble into nanoparticles (~93 nm) for delivering antitumor drugs, such as paclitaxel (PTX@PRES NPs). The system could achieve high drug loading (~7%) and overcome multidrug resistance (MDR). The results from the in vitro studies revealed that the NPs formed of PRES were stable in the systemic circulation, while could be efficiently degraded in tumor cells high GSH environment. Results from cytotoxicity tests confirmed that PTX@PRES NPs could effectively suppress the growth of cancer cells (A549) and drug-resistant cells (A549/PTX). The NPs could also be used to significantly increase the therapeutic efficacy of the drugs in A549/PTX tumor-bearing mice. In vivo investigations also demonstrated that the PRES-based NPs exhibited tumor inhibition effects. In summary, we report that the GSH-responsive polymer synthesized by us exhibited multiple interesting functions and could be used for effective drug delivery. The polymer exhibited good therapeutic effects and could be used to overcome MDR. Thus, the synthesized system can be used to develop a new strategy for treating cancer. [ABSTRACT FROM AUTHOR]

The purpose of this study is to investigate the chain mediating effects of networking behaviors and decision self-efficacy between work skills development and perceived employability. Structural equations modeling is used to analyze data collected from 813 Chinese students. The results show the following: first, the work skills development is positively correlated with perceived employability. Second, network behavior and decision self-efficacy each have a mediating effect between work skills development and perceived employability. Finally, this study found a chain mediating effect of network behavior and decision self-efficacy between work skills development and perceived employability. Therefore, this research shows that Work-Integrated Learning (WIL) needs to focus not only on skills development and employability outcomes but also on developing a strong network-based platform for stakeholders. In addition, higher education institutions and workplaces should also provide career guidance and counseling centers to help students build confidence in career decision-making and ensure students' mental health care and healthy career development. [ABSTRACT FROM AUTHOR]

Characterization of perfluorohexane sulfonate (PFHxS) isomers, a chemical proposed for listing under the Stockholm Convention, is important to elucidate its environmental behaviors and sources. Optimized chromatographic separation coupled with monitoring of the characteristic fragments enabled the identification of four mono-substituted and two di-substituted branched PFHxS isomers. The transitions of molecular ions m/z 399 to the fragments m/z 80 (n-), m/z 169 (iso-), m/z 319 (1m-), m/z 80 (2m-), and m/z 180 (3m-) were selected for quantifying the mono-substituted isomers. Method accuracy of the established LC-MS/MS was verified by comparing the results of technical products with those determined by 19F-nuclear magnetic resonance (NMR). The developed method was then used to quantify the isomeric compositions of PFHxS in the perfluorooctane sulfonate (PFOS) industrial products which contained PFHxS as an impurity, as well as in several kinds of water samples, with the limits of detection for all isomers in the range of 4 to 30 pg/L. For the first time, a liquid chromatography-tandem mass spectrometry method was established to separate and quantify the PFHxS isomers. The isomeric profiling of water samples suggested that PFHxS in the waters was mainly due to the direct contamination of PFHxS rather than from PFOS contamination. [ABSTRACT FROM AUTHOR]

Link prediction is one of the most important methods to uncover evolving mechanisms of dynamic complex networks. Determining these links raises well-known technical challenges in terms of weak correlation, uncertainty and non-stationary. In this paper, we presented a novel gated graph convolutional network (GCN) based on spatio-temporal semi-variogram (STEM-GCN). It learns spacial and temporal features in order to achieve link prediction in the dynamic networks. In this STEM-GCN model, we first utilized the spatio-temporal semi-variogram to obtain the spacial and temporal correlations from the dynamic networks. Its spacial correlation helped us determine the hyper-parameters of STEM-GCN and speed up its training. The correlation smoothing strategy is also introduced to eliminate the noise through temporal correlation and to improve the accuracy of link prediction. Finally, the network dynamics are captured by propagating the spacial and temporal features between consecutive time steps with stacked memory cell structures. The extensive experiments on real data sets demonstrated the effectiveness of the proposed approach for link prediction in dynamic complex networks. [ABSTRACT FROM AUTHOR]

To verify whether a graph is suitable for describing driver behaviour performance under the effects of navigation information, this study applies two types of prompt messages: simple and detailed. The simple messages contain only direction instructions, while the detailed messages contain distance, direction, road and lane instructions. A driving simulation experiment was designed to collect the empirical data. Two vehicle operating indicators (velocity and lateral offset), and two driver manoeuvre indicators (accelerator power and steering wheel angle) were selected, and T-test was used to compare the differences of behavioural performance. Driving behaviour graphs were constructed for the two message conditions; their characteristics and similarities were further analysed. Finally, the results of T-test of behavioural performance and similarity results of the driving behaviour graphs were compared. Results indicated that the two different types of prompt messages were associated with significant differences in driving behaviours, which implies that it is feasible to describe the characteristics of driving behaviours guided by navigation information using such graphs. This study provides a new method for systematically exploring the mechanisms affecting drivers' response to navigation information, and presents a new perspective for the optimisation of navigation information. [ABSTRACT FROM AUTHOR]

We developed a questionnaire for assessing urban community conflict (UCC) in China and tested its reliability and validity. A preliminary questionnaire was prepared based on interviews and a literature review. We conducted a pretest with 332 urban community residents and the results were processed using item analysis and exploratory factor analysis. The formal test involved another 378 urban community residents. The final UCC questionnaire included 25 items divided across three dimensions. The three-factor model had acceptable fit indices, internal consistency, and construct validity. Thus, the questionnaire can be used as a valid measurement tool for UCC research in China. [ABSTRACT FROM AUTHOR]

Rationale: The identification and evaluation of novel biomarkers are essential to clinical diagnosis and prognosis of colorectal cancer (CRC). Serum phosphopeptides have been recognized as a potential signature pool for cancers; therefore, we aim to profile the expression of serum phosphopeptides and to evaluate their feasibility in CRC diagnosis. Methods: We conducted the characterization and absolute quantification of endogenous phosphopeptides in sera using liquid chromatography–mass spectrometry analysis in combination with enrichment of phosphopeptides by ZrAs‐Fe3O4@SiO2nanoparticles and use of deuterium‐labeled standards. Differentially expressed analysis of four phosphopeptides was performed, generating a two‐phosphopeptide‐based biomarker, LF3–4, by logistic regression analysis, where LF3–4 is equal to (5.85 − 5.13 × [F3] − 3.57 × [F4]), and [F3] and [F4] are the concentration of phosphopeptides DpSGEGDFLAEGGGVR and ADpSGEGDFLAEGGGVR in sera, respectively. Results: The LF3–4 values showed significant difference in CRC cases compared with controls, and yielded a specificity of 100%, leading to correct classification of 56 (93%) out of 60 CRC patients, including 12 (92.3%) of 13 CRC cases in stage I. Double‐blind validation showed that 97.5% of CRC cases were discriminated accurately. Conclusions: The LF3–4 value was firstly verified to be a potential biomarker for CRC diagnosis, and may expand our view in underlying mechanisms for CRC. [ABSTRACT FROM AUTHOR]

Remote sensing (RS) plays an important role gathering data in many critical domains (e.g., global climate change, risk assessment and vulnerability reduction of natural hazards, resilience of ecosystems, and urban planning). Retrieving, managing, and analyzing large amounts of RS imagery poses substantial challenges. Google Earth Engine (GEE) provides a scalable, cloud-based, geospatial retrieval and processing platform. GEE also provides access to the vast majority of freely available, public, multi-temporal RS data and offers free cloud-based computational power for geospatial data analysis. Artificial intelligence (AI) methods are a critical enabling technology to automating the interpretation of RS imagery, particularly on object-based domains, so the integration of AI methods into GEE represents a promising path towards operationalizing automated RS-based monitoring programs. In this article, we provide a systematic review of relevant literature to identify recent research that incorporates AI methods in GEE. We then discuss some of the major challenges of integrating GEE and AI and identify several priorities for future research. We developed an interactive web application designed to allow readers to intuitively and dynamically review the publications included in this literature review. [ABSTRACT FROM AUTHOR]

Purpose: This study aimed to investigate the clinical usefulness of the enhanced-T1WI-based deep learning radiomics model (DLRM) in differentiating low- and high-grade meningiomas. Methods: A total of 132 patients with pathologically confirmed meningiomas were consecutively enrolled (105 in the training cohort and 27 in the test cohort). Radiomics features and deep learning features were extracted from T1 weighted images (T1WI) (both axial and sagittal) and the maximum slice of the axial tumor lesion, respectively. Then, the synthetic minority oversampling technique (SMOTE) was utilized to balance the sample numbers. The optimal discriminative features were selected for model building. LightGBM algorithm was used to develop DLRM by a combination of radiomics features and deep learning features. For comparison, a radiomics model (RM) and a deep learning model (DLM) were constructed using a similar method as well. Differentiating efficacy was determined by using the receiver operating characteristic (ROC) analysis. Results: A total of 15 features were selected to construct the DLRM with SMOTE, which showed good discrimination performance in both the training and test cohorts. The DLRM outperformed RM and DLM for differentiating low- and high-grade meningiomas (training AUC: 0.988 vs. 0.980 vs. 0.892; test AUC: 0.935 vs. 0.918 vs. 0.718). The accuracy, sensitivity, and specificity of the DLRM with SMOTE were 0.926, 0.900, and 0.924 in the test cohort, respectively. Conclusion: The DLRM with SMOTE based on enhanced T1WI images has favorable performance for noninvasively individualized prediction of meningioma grades, which exhibited favorable clinical usefulness superior over the radiomics features. [ABSTRACT FROM AUTHOR]

Background: As important enzymes regulating acetylation, histone deacetylases (HDACs) participate in a series of cell physiological process. However, the mechanisms responsible for individual HDAC family members in regulating innate immunity remained to be elucidated. Here we sought to reveal the mechanism of HDAC3 in regulating the inflammatory response of macrophages. Methods: RNAseq was done to detect the transcriptional influence of HDAC3 on macrophages. Kyoto Encyclopedia of Genes and Genomes was used to reveal the change of signaling pathways after HDAC3 knockout. CHIPseq was done to detect the deacetylation modification of HDAC3 on chromosome. Western blot, immunofluorescence, and real-time quantitative PCR were used to measure the change of genes and proteins' levels. Mice were intratracheal instillation with lipopolysaccharide or Pseudomonas aeruginosa to determine the influence of HDAC3 on inflammatory response in vivo. Results: HDAC3-deficient macrophages had increased expression of cathepsins resulting from elevated histone acetylation. Over-expressed cathepsins such as cathepsin B (CTSB) caused remarkable degradation of receptor (TNFRSF)-interacting serine-threonine kinase 1 (RIP1), which reduced TNFα mediated NF-κB activation and inflammatory response. Consistently, mice with macrophage specific knockout of HDAC3 were impaired in inflammatory response and thereby susceptible to Pseudomonas aeruginosa infection. Conclusion: HDAC3 was required for protecting RIP1 from degrading by CTSB in macrophages. Decreased RIP1 in HDAC3 knockout macrophages impaired TNFα mediated NF-κB activation. Our studies uncovered important roles of HDAC3 in the regulation of cathepsin-mediated lysosomal degradation and RIP1-mediated inflammatory response in macrophages as well as in host defense against bacterial infection. [ABSTRACT FROM AUTHOR]

Perfluoroalkyl carboxylic acids (PFCAs), a series of ubiquitous contaminants in the global environment, attracted much attention due to their potential for high bioaccumulation and toxicity to various organisms. There are a lot of measurement requests in currently increasing degradation studies of PFCAs, which usually rely on expensive liquid chromatography-mass spectrometry (LC-MS). The degradation solutions containing high-concentration PFCAs can easily cause the pipeline pollution of the LC/MS instrument, which is usually used for trace analysis of environmental samples. In this study, a simple and reliable precolumn derivatization LC method coupled with an ultraviolet detector (UV) was developed for the determination of the main PFCAs (C4-9) of environmental concern. These PFCAs in degradation solutions were crosslinked to UV-responsive 3, 4-diphenylamine (DCA) by a carbodiimidization method, followed by a simple solid-phase extraction (SPE) cleanup, and quantitatively measured using a conventional LC-UV instrument. Compared to previously reported precolumn derivatization methods, this new derivatization approach has the advantages such as mild reaction conditions, easy operation, enhanced stability of derivatives, and low cost. The instrumental limits of detection (ILDs) for the targeted PFCAs in organic and aqueous mediums were 0.2–0.5 and 0.6–1.5 mg/L, respectively. The method has been successfully applied to the determination of PFCAs in catalytic degradation solutions and recommended for use in other assays involving relatively high-concentration PFCAs. [ABSTRACT FROM AUTHOR]

Recurrence and metastasis of gastric cancer is a major therapeutic challenge for treatment. The presence of cancer stem cells (CSCs) is a major obstacle to the success of current cancer therapy, often leading to treatment resistance and tumor recurrence and metastasis. Therefore, it is important to develop effective strategies to eradicate CSCs. In this study, we developed a combined therapeutic strategy of photothermal therapy (PTT) and gastric cancer stem cells (GCSCs) inhibition by successfully synthesizing nanoliposomes loaded with IR780 (photosensitizer) and EN4 (c-Myc inhibitor). The nanocomposites are biocompatible and exhibit superior photoacoustic (PA) imaging properties. Under laser irradiation, IR780-mediated PTT effectively and rapidly killed tumor cells, while EN4 synergistically inhibited the self-renewal and stemness of GCSCs by suppressing the expression and activity of the pluripotent transcription factor c-Myc, preventing the tumor progression of gastric cancer. This Nano-EN-IR@Lip is expected to be a novel clinical nanomedicine for the integration of gastric cancer diagnosis, treatment and prevention. [Display omitted] • A combined therapeutic strategy of PTT and GCSC inhibition. • Nano-EN-IR@Lip loaded with IR780 (photosensitizer) and EN4 (c-Myc inhibitor). • IR780-mediated PTT; EN4 synergistically inhibited the self-renewal and stemness of GCSCs. • Nano-EN-IR@Lip is biocompatible and exhibit superior PA imaging properties. • A novel clinical nanomedicine for the integration of GC diagnosis, treatment and prevention. [ABSTRACT FROM AUTHOR]

Obesity and type 2 diabetes are significant risk factors for atherosclerotic cardiovascular disease (CVD) worldwide, but the underlying pathophysiological links are poorly understood. Neurotensin (NT), a 13-amino-acid hormone peptide, facilitates intestinal fat absorption and contributes to obesity in mice fed a high-fat diet. Elevated levels of pro-NT (a stable NT precursor produced in equimolar amounts relative to NT) are associated with obesity, type 2 diabetes, and CVD in humans. Whether NT is a causative factor in CVD is unknown. Nt +/+ and Nt –/– mice were either injected with adeno-associated virus encoding PCSK9 mutants or crossed with Ldlr –/– mice and fed a Western diet. Atherosclerotic plaques were analyzed by en face analysis, Oil Red O and CD68 staining. In humans, we evaluated the association between baseline pro-NT and growth of carotid bulb thickness after 16.4 years. Lipidomic profiles were analyzed. Atherosclerotic plaque formation is attenuated in Nt -deficient mice through mechanisms that are independent of reductions in circulating cholesterol and triglycerides but associated with remodeling of the plasma triglyceride pool. An increasing plasma concentration of pro-NT predicts atherosclerotic events in coronary and cerebral arteries independent of all major traditional risk factors, indicating a strong link between NT and atherosclerosis. This plasma lipid profile analysis confirms the association of pro-NT with remodeling of the plasma triglyceride pool in atherosclerotic events. Our findings are the first to directly link NT to increased atherosclerosis and indicate the potential role for NT in preventive and therapeutic strategies for CVD. [Display omitted] • NT deficiency ameliorates atherosclerotic lesions. • Shorter and more saturated fatty acids are decreased in Nt-deficient mice. • Plasma pro-NT predicts atherosclerotic events in coronary and cerebral arteries. • pro-NT predicts atherosclerotic events independent of traditional risk factors. • pro-NT is associated with triglyceride pool remodeling in atherosclerotic events. [ABSTRACT FROM AUTHOR]

Irisin plays a crucial role in the glucose metabolism of mammals and fish. However, the mechanism by which irisin regulates hepatic glucose metabolism is not entirely known. To clarify irisin's function and underlying mechanisms in common carp glucose metabolism, the FNDC5 expression patterns were detected after insulin/glucagon injection, long-term high-glucose, and high-fat diet feeding. In vivo, the activity of PFK and the synthesis of liver glycogen were significantly increased, while the enzyme activity of PEPCK was reduced after intraperitoneal injection with irisin. In contrast, irisin siRNA attenuated the brain and liver FNDC5a and FNDC5b expression, and the serum glucose was increased. Glut2, gs, hk, and pfk were considerably downregulated in the brain and liver, while pygl, pepck, and g6pase were remarkably upregulated after siRNA treatment. In vitro, irisin activates the phosphorylation level of AMPK and PI3K/Akt in primary hepatocytes. Compound C and wortmannin inhibit AMPK and PI3K/Akt, reversing irisin-induced expression of g6pase, fbpase, pepck, gk, gs, pfk, hk, and gsk3b. At the same time, compound C attenuated irisin-induced expression of pi3k and akt. Our research reveals the mechanisms of irisinA and irisinB as glucose metabolism regulators in teleost for the first time. [ABSTRACT FROM AUTHOR]

The issue of students' career choice is the common concern of students themselves, parents, and educators. However, students' behavioral data have not been thoroughly studied for understanding their career choice. In this study, we used eXtreme Gradient Boosting (XGBoost), a machine learning (ML) technique, to predict the career choice of college students using a real-world dataset collected in a specific college. Specifically, the data include information on the education and career choice of 18,000 graduates during their college years. In addition, SHAP (Shapley Additive exPlanation) was employed to interpret the results and analyze the importance of individual features. The results show that XGBoost can predict students' career choice robustly with a precision, recall rate, and an F1 value of 89.1%, 85.4%, and 0.872, respectively. Furthermore, the interaction of features among four different choices of students (i.e., choose to study in China, choose to work, difficulty in finding a job, and choose to study aboard) were also explored. Several educational features, especially differences in grade point average (GPA) during their college studying, are found to have relatively larger impact on the final choice of career. These results can be of help in the planning, design, and implementation of higher educational institutions' (HEIs) events. [ABSTRACT FROM AUTHOR]

[Display omitted] • The n - n type BiOIO 3 /BBN heterojunction is prepared by one-step hydrothermal method. • The photocatalytic activity of the composite is better than that of TiO 2 under UV light. • Singlet oxygen plays a decisive role in the degradation reaction. • BiOIO 3 /BBN has a high stability and long-time reusability. • Two possible degradation pathways are proposed. The heterojunction photocatalyst, BiOIO 3 /[Bi 6 O 6 (OH) 3 ](NO 3) 3 ·1.5H 2 O (BiOIO 3 /BBN), was successfully synthesized by a simple one-step hydrothermal method. The results showed that under UV light irradiation, the formation of a heterojunction could greatly enhance the photocatalytic efficiency of the prepared catalyst for bisphenol A (BPA). The BiOIO 3 /BBN heterostructure had the best reaction rate constant, which was 81.82 times, 1.52 times, and 43.40 times improvement of TiO 2 , BiOIO 3 , and BBN respectively. Through the free radical capture experiments and electron spin resonance spectroscopy, it was conducted that 1O 2 , h+, e-, •OH and •O 2 – were reactive species in the process of photocatalytic degradation of BPA. The photocatalytic mechanism was further investigated and confirmed that the BiOIO 3 /BBN heterojunction could improve the separation and transfer of photo-generated carriers, thereby greatly enhancing the catalytic efficiency. The degradation products of BPA were detected by HPLC-MS, and the degradation reaction pathway was deduced. [ABSTRACT FROM AUTHOR]

Genetic variants that regulate lipid phosphate phosphatase 3 (LPP3) expression are risk factors for the development of atherosclerotic cardiovascular disease. LPP3 is dynamically upregulated in the context of vascular inflammation with particularly heightened expression in smooth muscle cells (SMC), however, the impact of LPP3 on vascular pathology is not fully understood. We investigated the role of LPP3 and lysophospholipid signaling in a well-defined model of pathologic aortic injury and observed Angiotensin II (Ang II) increases expression of PLPP3 in SMCs through nuclear factor kappa B (NF-κB) signaling Plpp3 global reduction (Plpp3+/−) or SMC-specific deletion (SM22-Δ) protects hyperlipidemic mice from AngII-mediated aneurysm formation. LPP3 expression regulates SMC differentiation state and lowering LPP3 levels promotes a fibroblast-like phenotype. Decreased inactivation of bioactive lysophosphatidic acid (LPA) in settings of LPP3 deficiency may underlie these phenotypes because deletion of LPA receptor 4 in mice promotes early aortic dilation and rupture in response to AngII. LPP3 expression and LPA signaling influence SMC and vessel wall responses that are important for aortic dissection and aneurysm formation. These findings could have important implications for therapeutics targeting LPA metabolism and signaling in ongoing clinical trials. [ABSTRACT FROM AUTHOR]

Water features (e.g., water quantity and water quality) are one of the most important environmental factors essential to improving climate-change resilience. Remote sensing (RS) technologies empowered by artificial intelligence (AI) have become one of the most demanded strategies to automating water information extraction and thus intelligent monitoring. In this article, we provide a systematic review of the literature that incorporates artificial intelligence and computer vision methods in the water resources sector with a focus on intelligent water body extraction and water quality detection and monitoring through remote sensing. Based on this review, the main challenges of leveraging AI and RS for intelligent water information extraction are discussed, and research priorities are identified. An interactive web application designed to allow readers to intuitively and dynamically review the relevant literature was also developed. [ABSTRACT FROM AUTHOR]

Background: Small interfering RNAs (siRNAs) target homologous genomic DNA sequences for cytosine methylation, known as RNA-directed DNA methylation (RdDM), plays an important role in transposon control and regulation of gene expression in plants. Repressor of silencing 1 (ROS1) can negatively regulate the RdDM pathway. Results: In this paper, we investigated the molecular mechanisms by which an upstream regulator ACD6 in the salicylic acid (SA) defense pathway, an ABA pathway-related gene ACO3, and GSTF14, an endogenous gene of the glutathione S-transferase superfamily, were induced by various abiotic stresses. The results demonstrated that abiotic stresses, including water deficit, cold, and salt stresses, induced demethylation of the repeats in the promoters of ACD6, ACO3, and GSTF14 and transcriptionally activated their expression. Furthermore, our results revealed that ROS1-mediated DNA demethylation plays an important role in the process of transcriptional activation of ACD6 and GSTF14 when Arabidopsis plants are subjected to cold stress. Conclusions: This study revealed that ROS1 plays an important role in the molecular mechanisms associated with genes involved in defense pathways in response to abiotic stresses. [ABSTRACT FROM AUTHOR]

This study was aimed at exploring the new management mode of medical information processing and emergency first aid nursing management under the new artificial intelligence technology. This study will use the artificial intelligence algorithm to optimize medical information processing and emergency first aid nursing management process, in order to improve the efficiency of emergency department and first aid efficiency. The successful rescue rates of hemorrhagic shock, coma, dyspnea, and more than three organs injury were 96.7%, 92.5%, 93.7%, and 87.2%, respectively, after the emergency first aid nursing mode was used in the hospital emergency center. The success rates of first aid within three years were compared, which were 91.8%, 93.4%, and 94.2%, respectively, showing an increasing trend year by year. 255 emergency patients in five batches in June and five batches in July were selected as the research objects by convenience sampling method. Among them, 116 cases in June were taken as the experimental group, and 139 cases in July were taken as the control group, which was used to verify the efficiency of the design model in this study. The results showed that the triage time of the two groups was 8.16 ± 2.07 min and 19.21 ± 6.36 min, respectively, and the difference was statistically significant (P < 0.01). The triage coincidence rates were 96.35% and 90.04%, respectively, and the difference was statistically significant (P < 0.05). The research proved that the design of intelligent medical information processing and emergency first aid nursing management research model can effectively improve the triage efficiency of the wounded, assist the efficiency of emergency nursing of medical staff, and improve the survival rate of emergency patients, which is worthy of clinical promotion. [ABSTRACT FROM AUTHOR]

In the present paper, we study the Newton polygons for the L-functions of n-variable generalized Kloosterman sums. Generally, the Newton polygon has a topological lower bound, called the Hodge polygon. In order to determine the Hodge polygon, we explicitly construct a basis of the top-dimensional Dwork cohomology. Using Wan's decomposition theorem and diagonal local theory, we obtain when the Newton polygon coincides with the Hodge polygon. In particular, we concretely get the slope sequence for the L-function of F ¯ ⁢ (λ ¯ , x) := ∑ i = 1 n x i a i + λ ¯ ⁢ ∏ i = 1 n x i - 1 , \bar{F}(\bar{\lambda},x):=\sum_{i=1}^{n}x_{i}^{a_{i}}+\bar{\lambda}\prod_{i=1}% ^{n}x_{i}^{-1}, with a 1 , ... , a n {a_{1},\ldots,a_{n}} being pairwise coprime for n ≥ 2 {n\geq 2}. [ABSTRACT FROM AUTHOR]