Search

[Display omitted] Porous carbon materials are highly desirable for removing benzene due to their low energy for capture and regeneration. Research has demonstrated that narrow microporous volume is crucial for effective adsorption of benzene at ultra-low concentration. Unfortunately, achieving directional increase in the narrow microporous volume in porous carbon remains a challenge. Here, nitrogen-doped hydrothermal carbon was prepared using urea-assisted hydrothermal method, and then porous carbon (PUC800) was prepared by KOH activation. The resulting material had 180 % higher pore volume and 179 % higher surface area compared to non-nitrogen activation methods. Then, using mechanochemical (mechanical compaction and KOH activation) approach to produce PUC800-3T, which had a 30 % increase in pore volume and a 33 % increase in surface area compared to PUC800. PUC800-3T showed benzene adsorption capacity of 4.2 mmol g−1 at 1 Pa and 5.8 mmol g−1 at 5 Pa. Experimental and molecular simulation indicate that the benzene adsorption at 1 and 5 Pa is determined by pore volume of less than 0.8 and 0.9 nm, respectively. Density functional theory calculations provided insight into the C H⋯X (X = N/O) interactions drive benzene adsorption on the carbon framework. This work provides valuable theoretical and experimental support for designing, preparing, and applying adsorbents for trace removal of benzene vapor. [ABSTRACT FROM AUTHOR]

Background: Accumulating evidence supports the involvement of adaptive immunity in the development of radiation‐induced brain injury (RIBI). Our previous work has emphasized the cytotoxic function of CD8+ T cells in RIBI. In this study, we aimed to investigate the presence and potential roles of cytotoxic CD4+ T cells (CD4+ CTLs) in RIBI to gain a more comprehensive understanding of adaptive immunity in this context. Main Text: Utilizing single‐cell RNA sequencing (scRNA‐seq), we analyzed 3934 CD4+ T cells from the brain lesions of four RIBI patients and identified six subclusters within this population. A notable subset, the cytotoxic CD4+ T cells (CD4+ CTLs), was marked with high expression of cytotoxicity‐related genes (NKG7, GZMH, GNLY, FGFBP2, and GZMB) and several chemokine and chemokine receptors (CCL5, CX3CR1, and CCL4L2). Through in‐depth pseudotime analysis, which simulates the development of CD4+ T cells, we observed that the CD4+ CTLs exhibited signatures of terminal differentiation. Their functions were enriched in protein serine/threonine kinase activity, GTPase regulator activity, phosphoprotein phosphatase activity, and cysteine‐type endopeptidase activity involved in the apoptotic signaling pathway. Correspondingly, mice subjected to gamma knife irradiation on the brain showed a time‐dependent infiltration of CD4+ T cells, an increase of MHCII+ cells, and the existence of CD4+ CTLs in lesions, along with an elevation of apoptotic‐related proteins. Finally, and most crucially, single‐cell T‐cell receptor sequencing (scTCR‐seq) analysis at the patient level determined a large clonal expansion of CD4+ CTLs in lesion tissues of RIBI. Transcriptional factor‐encoding genes TBX21, RORB, and EOMES showed positive correlations with the cytotoxic functions of CD4+ T cells, suggesting their potential to distinguish RIBI‐related CD4+ CTLs from other subsets. Conclusion: The present study enriches the understanding of the transcriptional landscape of adaptive immune cells in RIBI patients. It provides the first description of a clonally expanded CD4+ CTL subset in RIBI lesions, which may illuminate new mechanisms in the development of RIBI and offer potential biomarkers or therapeutic targets for the disease. [ABSTRACT FROM AUTHOR]

Bitterness is a basic flavor in alcoholic beverages (beer, wine, liquor, and rice wine) and has physiological significance, although excessive and disharmonious bitterness is unpleasant. Current research on flavor in alcoholic beverages is primarily focused on volatile constituents and distinctive flavor compounds. However, studies on the recognition, identification, and metabolic mechanism of bitter substances are still in the preliminary phase. This review provides an integrated account of the signal transduction and recognition, composition and sensory properties of bitterness in alcoholic beverages. Abiotic and biotic factors within fermentation systems that may influence the bitterness of the fermented beverages are summarized. Future research on the molecular characteristics, taste attributes, and metabolic pathways of bitterness are discussed herein. Polyphenol and iso- α -acid extracted from grapes and hops are the typical bitter substances in wine and beer. Microbial metabolites, including bitter peptides and higher alcohols, are the primary contributors to bitterness in liquor and rice wine. These compounds can activate one or more specific bitter taste receptors. Manufacturing processes and sensory interactions also influence the perception of bitterness. Establishing normative bitterness scoring methods and distinguishing acceptable and unacceptable bitterness based on sensory physiology will continue to be the goals of bitterness research. Further elucidation of the metabolic mechanism underlying bitterness through a combination of omics and synthetic microbiology will allow for taste characteristics of alcoholic beverages to be manipulated. Image 1 • The bitterness profile in alcoholic beverages was described. • Polyphenols, iso-α-acids, higher alcohols, and bitter peptides are the major bitter compounds. • Raw materials and microbial metabolism were the major contributor to bitterness. • Improvement on bio-electronic sensor to identify bitter components was suggested. • The bitterness metabolic mechanism is needed further microbial exploration. [ABSTRACT FROM AUTHOR]

Motivated by the promising benefits of dynamic time division duplex (TDD), in this paper, we use a unified framework to investigate both the technical issues of applying dynamic TDD in homogeneous small-cell networks (HomSCNs) and the feasibility of introducing dynamic TDD into heterogeneous networks (HetNets). First, HomSCNs are analyzed, and a small-cell base-station (BS) scheduler that dynamically and independently schedules downlink (DL) and uplink (UL) subframes is presented, such that load balancing between the DL and UL traffic can be achieved. Moreover, the effectiveness of various interlink interference mitigation (ILIM) schemes and their combinations is systematically investigated and compared. Moreover, the interesting possibility of partial interference cancelation (IC) is also explored. Second, based on the proposed schemes, the joint operation of dynamic TDD together with cell range expansion (CRE) and almost blank subframe (ABS) in HetNets is studied. In this regard, scheduling polices in small cells and an algorithm to derive the appropriate macrocell traffic offload and ABS duty cycle under dynamic TDD operation are proposed. Moreover, the full IC and the partial IC schemes are investigated for dynamic TDD in HetNets. The user-equipment (UE) packet throughput performance of the proposed/discussed schemes is benchmarked using system-level simulations. [ABSTRACT FROM AUTHOR]

Introduction. Radiotherapy for patients with head and neck cancers raises their risk of aspiration pneumonia-related death. We aimed to develop and validate a model to predict radiation-associated aspiration pneumonia (RAP) among patients with dysphagia after radiotherapy for nasopharyngeal carcinoma (NPC). Materials and Methods. A total of 453 dysphagic patients with NPC were retrospectively recruited from Sun Yat-Sen Memorial Hospital from January 2012 to January 2018. Patients were randomly divided into training cohort (n = 302) and internal validation cohort (n = 151) at a ratio of 2 : 1. The concordance index (C-index) and calibration curve were used to evaluate the accuracy and discriminative ability of this model. Moreover, decision curve analysis was performed to evaluate the net clinical benefit. The results were externally validated in 203 dysphagic patients from the First People's Hospital of Foshan. Results. Derived from multivariable analysis of the training cohort, four independent factors were introduced to predict RAP, including Kubota water drinking test grades, the maximum radiation dose of lymph node gross tumor volume (Dmax of the GTVnd), neutrophil count, and erythrocyte sedimentation rate (ESR). The nomogram showed favorable calibration and discrimination regarding the training cohort, with a C-index of 0.749 (95% confidence interval (CI), 0.681 to 0.817), which was confirmed by the internal validation cohort (C-index 0.743; 95% CI, 0.669 to 0.818) and the external validation cohort (C-index 0.722; 95% CI, 0.606 to 0.838). Conclusions. Our study established and validated a simple nomogram for RAP among patients with dysphagia after radiotherapy for NPC. [ABSTRACT FROM AUTHOR]

Organic acids are essential constituents of bran vinegar, significantly influencing its sensory quality and functional characteristics. However, until now, little has been known about organic acid synthesis in bran vinegar. This study investigated the main physicochemical properties, organic acid composition, and changes in microbial diversity throughout the fermentation of Sichuan bran vinegar. Additionally, the functional microorganisms related to organic acid synthesis were analyzed. The results showed the presence of eight organic acids in bran vinegar. Amino acid nitrogen, reducing sugars, and alcohol content were identified as the main physicochemical factors contributing to organic acid formation. The dominant microorganisms associated with organic acids during fermentation included Lactobacillus, Acetobacter, Bacillus, Alternaria, Pichia, and Candida. Microbial communities related to specific organic acids during the fermentation of vinegar grains were identified using the two-way orthogonal partial least squares (O2PLS) model. Bacterial communities showed stronger correlations with the principal organic acids. These findings provided the basis for analyzing the formation of flavor compounds in the bran vinegar fermentation process. • There are eight main types of organic acids in bran vinegar. • Six dominant microorganisms are key to organic acids production. • Bacterial communities are more important to organic acids accumulation. [ABSTRACT FROM AUTHOR]

Abstract: Histone deacetylase inhibitors are promising chemotherapeutic agents and some are in clinical trials. Several molecular mechanisms have been invoked to describe their effects on cancer cells in vivo and in vitro. Autophagy has been observed in response to several anticancer reagents and has been demonstrated to be responsible for cell death. However, the exact mechanism of this phenomenon is still not clear. Here we demonstrated that suberoylanilide hydroxamic acid, a histone deacetylase inhibitor, induces nonapoptotic cell death with several specific features characteristic of autophagy in Hela S3 cells. Suberoylanilide hydroxamic acid inhibits the activity of the mammalian target of rapamycin, a negative regulator of macroautophagy which induces the formation of autophagosomes in a Beclin 1- and autophagy-related 7-dependent manner. This process is mediated by Akt and tuberous sclerosis 2 as is demonstrated by inhibition by continuous active Akt plasmid transfection and RNA interference of tuberous sclerosis 2. Our data provide the first evidence that suberoylanilide hydroxamic acid induces autophagy in Hela S3 cells through interference with the mammalian target of rapamycin signaling pathway. These findings suggest that suberoylanilide hydroxamic acid may induce autophagic cancer cell death via its specific pathway, and invite further investigation into the detailed mechanism of this pathway to explore this compound''s full potential as a chemotherapeutic agent. [Copyright &y& Elsevier]