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One of the causes of topical inflammation is UV-B radiation, which causes erythema and edema of the skin. Zingiber montanum rhizome in Indonesia is known as a natural ingredient that has the potential to treat skin redness. This study was conducted to isolate and identify the active compound, as well as to determine the protective activity against inflammatory of isolate in BALB/c mice exposed to UV-B. The isolation stage begins with the extraction process by maceration, fractionation by liquid-liquid partition and chromatography. Identification by IR Spectrophotometry, C-NMR, and H-NMR. The protection test against inflammation used a mice skin model irradiated with UV-B, with 5 test groups, namely: control, UV-B, base, isolate, and positive control 2.5% hydrocortisone cream group. Statistical data analysis using a different test. The results showed that the isolates could be separated from fraction number 8. The partial structure of the isolates were aromatic compounds having hydroxyl and methoxy groups, which leads to the structure of ferulic acid compounds. The dorsal skinfold thickness of mice in the control, UV-B, base, isolate, and positive control groups of 2.5% hydrocortisone cream were 0.570 ± 0.056; 1.612 ± 0.250; 1.700 ± 0.556; 0.952 ± 0.127; 0.824 ± 0.144 cm, respectively. There were significant differences between all test groups. In this study, an active compound was isolated from Zingiber montanum rhizome which has the potential to provide protective activity against inflammation in mice skin models exposed to UV-B. [ABSTRACT FROM AUTHOR]

UV radiation, environmental pollution, stress conditions can cause skin disorders that lead to premature aging. An antioxidant bio-cellulose mask is an option to reduce the premature aging process by using environmentally friendly materials. Natural antioxidants can be obtained from plant extract that contains phenols and flavonoids, which are found in Camellia sinensis such as black tea and red tea. The purposes of this research were to determine the total phenol and total flavonoid content of black tea and red tea extracts; to determine the physical characteristics of the essence of black tea and red tea extracts, and to know the antioxidant activity of the bio-cellulose mask extract qualitatively. Extracts were prepared using ethanol 70% with the maceration method. Total phenol and total flavonoid contents were determined with the colorimetric method. Essence physical characteristics test in the form of pH, homogeneity, viscosity, and primary irritation index. Bio-cellulose mask was obtained by fermenting coconut water using Acetobacter xylinum bacteria. The antioxidant activity of the mask was determined qualitatively using 1,1-diphenyl-2picrylhidrazyl (DPPH). The total phenol content of black tea and red tea extracts were 227.91±6.39 and 184.77±9.18 mg GAE/g extract, while the total flavonoid content of 2.71±0.006 and 2.35±0.015 mg QE/g extract. The essence of extracts was homogeneous; pH in the range of 5.42 – 5.59; viscosity of 501.50 and 634.10 cps. The primary irritation index values of essences were less than 0.05. The bio-cellulose mask showed qualitative antioxidant activity with DPPH. It can be concluded that black tea and red tea extracts are potential ingredients in the development of the bio-cellulose mask. [ABSTRACT FROM AUTHOR]