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We report results on the mechanical and structural response to shear deformation of nanodiamond superstructures in interlayer-bonded twisted bilayer graphene (IB-TBG) and interlayer-bonded graphene bilayers with randomly distributed individual interlayer C–C bonds (RD-IBGs) based on molecular-dynamics simulations. We find that IB-TBG nanodiamond superstructures subjected to shear deformation undergo a brittle-to-ductile transition (BDT) with increasing interlayer bond density (nanodiamond fraction). However, RD-IBG bilayer sheets upon shear deformation consistently undergo brittle failure without exhibiting a BDT. We identify, explain, and characterize in atomic-level detail the different failure mechanisms of the above bilayer structures. We also report the dependence of the mechanical properties, such as shear strength, crack initiation strain, toughness, and shear modulus, of these graphene bilayer sheets on their interlayer bond density and find that these properties differ significantly between IB-TBG nanodiamond superstructures and RD-IBG sheets. Our findings show that the mechanical properties of interlayer-bonded bilayer graphene sheets, including their ductility and the type of failure they undergo under shear deformation, can be systematically tailored by controlling interlayer bond density and distribution. These findings contribute significantly to our understanding of these 2D graphene-based materials as mechanical metamaterials. [ABSTRACT FROM AUTHOR]

This study explores tourists’ perceptions of the Sigiriya UNESCO World Heritage Site in Sri Lanka. The study has used TripAdvisor reviews and analysed using content analysis employing the 5’A’s framework for destinations (Attractions, Accessibility, Amenities, Activities, Accommodation). The results indicate that tourists perceive the attraction based on scenery, historical significance, features, cost, and crowd levels. Their perception highlighted the availability of accommodation, facilities and amenities. The activities including rock climbing, garden visits, photography, and artefact observation along with the accessibility considerations, such as fitness requirements, were also highlighted in their perceptions. The study revealed a key finding that ‘authentic experience’ is a crucial component of heritage tourism. Accordingly, this study proposes expanding 5’A’s framework incorporating ‘authentic experiences’ to understand tourists’ perceptions and their recommendation behaviour. Practical implications include preserving the site’s natural and historical value, improving accessibility and amenities, and offering authentic experiences in heritage destinations to enhance tourists’ perceptions [ABSTRACT FROM AUTHOR]

With the advancement of modern construction technology, reinforced concrete (RC) has become the most widely used composite construction material. Corrosion of embedded reinforcement bars in RC structures, which is essentially the result of carbonation and chloride attack, is regarded as one of the weaknesses. Hence, many research studies have been conducted to evaluate the effect of corrosion on the service life of RC structures. However, so far no proper equation has been derived to predict the extent of carbonation-induced corrosion. Identifying this research gap, the present study intends to derive an equation for the coefficient of carbonation (K), which is the governing measure of the rate of carbonation. This paper identifies concrete grade, exposed CO2 gas concentration, and exposed Relative Humidity (RH) level as the major parameters affecting the carbonation process. The behaviour of each factor along with K was identified by conducting a modified accelerated carbonation test (ACT). These experimentally derived K values were statistically analysed under the principle of multiple linear regression, to derive an equation for predicting K values for RC structures. Based on the results, this paper proposes two user-friendly mathematical models covering the entire environmental RH range to predict the K values of RC structures. [ABSTRACT FROM AUTHOR]

ABSTRACT Humanity has a longstanding reliance on natural plants for medicinal purposes, and Gymnema lactiferum (G. lactiferum) has emerged as a medicinal plant with deep‐rooted traditional usage. Throughout history, this plant has been an integral part of traditional medical systems, demonstrating diverse therapeutic effects. Notably, among these effects is its ability to decrease blood glucose concentration in diabetic patients, impart cooling effects, serve as an anabolic and rehydrating agent, stimulate spermiogenesis, and exhibit wormicidal properties. Furthermore, G. lactiferum has been used in treating conditions such as hemorrhoids cancers, anorexia, and as a cardiac stimulant. The primary objective of this review is to comprehensively gather and critically assess research findings regarding the medicinal properties of G. lactiferum, specifically emphasizing the bioactive compounds responsible for these properties. Previous studies have documented the presence of various phytochemicals in G. lactiferum, which are associated with some biological activities, including antioxidative, anti‐hyperglycemic, cholesterol‐regulating, and anti‐inflammatory properties. Additionally, this review explores potential future applications for this plant. Beyond its medicinal significance, extracts derived from G. lactiferum demonstrate promise for future nutritional applications. This review highlights the potential use of G. lactiferum as an herbal medicine by critically assessing research on its medicinal value. [ABSTRACT FROM AUTHOR]

Background: Urinary tract infections (UTIs) are the second most common infection, affecting 150 million people each year worldwide. Enterobacteriaceae species expressing extended-spectrum β-lactamases (ESBLs) are on the rise across the globe and are becoming a severe problem in the therapeutic management of clinical cases of urinary tract infection. Knowledge of the prevalence and antibiogram profile of such isolates is essential to develop an appropriate treatment methodology. This study aimed to investigate the prevalence of Enterobacteriaceae isolates exhibiting ESBL and their selective oral antibiogram profile at the district general hospital, Polonnaruwa. Results: A total of 4386 urine specimens received to the Microbiology Laboratory during the study period. Among them, 1081 (24.6%) showed positive results for urine culture while 200/1081 specimens showed ESBL isolates. Out of the selected 200 specimen's majority (67.5%) of samples received from the In-Patient Department. There were 200 patients and reported that 115 (57.5%) were females and 85 (42.5%) were males. The majority (51%) of the patients belong to the age group of 55–74 years. Among the ESBLs positive specimens, the majority 74.5% (n = 149) identified organisms were E. coli followed by Klebsiella spp.17.5% (n = 35), Enterobacteriaceae 7% (n = 14) and only1% (n = 2) isolate of Proteus spp. Mecillinam (87.92%) and Nitrofurantoin (83.2%) showed higher effectiveness against E. coli. Nitrofurantoin showed the highest effectiveness against Klebsiella spp. (40%), other Enterobacteriaceae spp. (100%). Proteus spp. showed 100% effectiveness and resistance respectively against Ciprofloxacin, Cotrimoxazole and Nitrofurantoin. Conclusion: The most predominant ESBLs producing uro-pathogen was the E. coli in the study setting and E. coli had higher sensitivity rate against Mecillinam. Among currently used oral antibiotics Nitrofurantoin was the best choice for UTIs caused by ESBL producers. [ABSTRACT FROM AUTHOR]

Background: Pauci‐immune glomerulonephritis (GN) due to antineutrophil cytoplasmic antibody (ANCA)‐associated vasculitis (AAV) is a common cause of crescentic GN. Despite advances in treatment, rates of mortality and progression to end‐stage kidney disease remain high. Renal involvement is diagnosed by histological examination of kidney tissue. Serum ANCAs play a significant role in AAV; however, the value of serum ANCA quantification to predict renal involvement is not well‐established. Aim: We aimed to evaluate the diagnostic accuracy of serum ANCA titres in diagnosing AAV with renal involvement. Methods: We conducted a retrospective study of consecutive native kidney biopsies reported at our centre from 2016 to 2021. We included all adults who had both a kidney biopsy and ANCA serology. ANCA serology was tested using indirect immunofluorescence with reporting of titres. Antibodies to proteinase 3 and myeloperoxidase were measured using a chemiluminescent immunoassay. Results: Eight hundred and forty‐eight native kidney biopsies were reported during the study period. Five hundred and seven cases were included. The biopsy prevalence of pauci‐immune GN in paired samples was 41/507 (8.1%). Most of the cohort had haematuria (66.6%), proteinuria (93.4%) and/or acute kidney injury (65.0%). A positive ANCA at any titre demonstrated a sensitivity of 97.6% and a specificity of 71.2% for a diagnosis of pauci‐immune GN. The area under the curve for the receiver operator characteristic was 0.93 (95% confidence interval [CI]: 0.89–0.97). A cutoff ANCA titre of 1:160 provided the optimum balance between a sensitivity of 75.6% (95% CI: 59.7%–87.6%) and a specificity of 94.0% (95% CI: 91.6%–96.0%). Conclusions: ANCA titres are highly predictive of pauci‐immune GN in the appropriate context. While serum ANCA quantitation may not replace renal biopsy, reporting will assist in the decision to start treatment early for patients with organ or life‐threatening disease. [ABSTRACT FROM AUTHOR]

Sleep disorders are prevalent neurological conditions linked to neurocognitive impairments. Understanding the neuroplasticity changes in the hippocampus, which plays a central role in regulating neurocognitive function, is crucial in the context of sleep disorders. However, research on neurodegenerative disorders and the influence of sleep disorders on hippocampal neuroplasticity remains largely unclear. Therefore, this review aims to highlight the latest advancements regarding hippocampal neuroplasticity and functional changes during sleep disorders, drawing insights from clinical and preclinical research involving sleep-deprived animal models. These articles were gathered through comprehensive literature searches across databases, including Google Scholar, PubMed, Web of Science, and Scopus. Maternal sleep deprivation has been observed to cause neurocognitive impairment in offspring, along with changes in protein expression levels associated with neuroplasticity. Similarly, sleep deprivation in adult mice has been shown to affect several cognitive functions and fear extinction without influencing the acquisition of fear conditioning. While mechanistic research on neurocognitive dysfunction induced by maternal and adult sleep deprivation is limited, it suggests the involvement of several signaling pathways, including neurotrophic factors, synaptic proteins, and inflammatory molecules, which are triggered by sleep deprivation. Further studies are needed to clarify the mechanistic pathways underlying hippocampal dysfunction and synaptic alterations associated with sleep disturbances. [ABSTRACT FROM AUTHOR]

Solvents are essential for chemical synthesis and material preparation; however, traditional solvents face challenges in meeting safety and sustainability standards. Consequently, there is a growing focus on environmentally friendly solvent systems for polymeric materials, with deep eutectic solvents (DESs) standing out as promising alternatives owing to their eco-friendly attributes. In this review, we summarize studies on this novel solvent system in a broad range of applications, where DESs play multiple roles in polymer synthesis and corresponding material fabrication. In detail, we focus on the utilization of DESs in the synthesis of green polymers and related functional polymeric materials ranging from soft materials such as gels and nanofibers to membranes, films, and complex architectures generated from various techniques such as 3D printing, electrospinning, and self-assembly, together with their properties. Moreover, we discuss the green credentials through sustainability analysis and life-cycle assessment of DES-based polymeric materials. We believe this review will inspire more investigation into the use of DESs for functional green polymeric materials towards a sustainable future. [ABSTRACT FROM AUTHOR]

The effects of CFRP wrapping length and the beam curvature on the ultimate strength of carbon fibre reinforced polymer (CFRP)-strengthened in-plane curved steel circular hollow sections (SCHS) were studied by means of an experimental study. A total of 16 beams having four curvatures 0 mm, 2000 mm, 4000 mm and 6000 mm, which were strengthened with three CFRP lengths 500 mm, 750 mm and 1000 mm, were tested under three-point bending. Failure loads, failure mechanisms, strain variations and load-deflection responses were observed. Four types of failure modes were observed in CFRP-strengthened vertically curved SCHS depending on the CFRP wrapping length. The best performance was noted in strengthened beams when the ratio between CFRP wrapping length and beam span is 0.625. [ABSTRACT FROM AUTHOR]

In this paper, we introduce a new method based on a library of chemical reactions for constructing a system of ordinary differential equations from stochastic simulations arising from an agent-based model. The advantage of this approach is that this library respects any coupling between systems components, whereas the SINDy algorithm (introduced by Brunton et al.) treats the individual components as decoupled from one another. Another advantage of our approach is that we can use a non-negative least squares algorithm to find the non-negative rate constants in a very robust, stable and simple manner. We illustrate our ideas on an agent-based model of tumour growth on a 2D lattice. [ABSTRACT FROM AUTHOR]

Background: Antimicrobial overprescription is common for lower respiratory tract infections (LRTI), as viral and bacterial infections generally present with similar clinical features. Overprescription is associated with downstream antimicrobial resistance. This study aims to identify the prevalence and predictors of antibiotic prescription among patients hospitalized with viral LRTI. Methods: A prospective cohort study was conducted among patients aged ≥1 year hospitalized with viral LRTI in a tertiary care hospital in Southern Province, Sri Lanka from 2018–2021. Demographic, clinical, and laboratory data were recorded. Nasopharyngeal and blood samples were collected for multiplex polymerase chain reaction testing for 21 respiratory pathogens and procalcitonin (PCT) detection, respectively. Demographic and clinical features associated with antibiotic prescription were identified using Chi Square and t-tests; significant variables (p<0.05) were further included in multivariable logistic regression models. The potential impact of biomarker testing on antibiotic prescription was simulated using standard c-reactive protein (CRP) and PCT cut-offs. Results: Of 1217 patients enrolled, 438 (36.0%) had ≥1 respiratory virus detected, with 48.4% of these patients being male and 30.8% children. Influenza A (39.3%) and human rhinovirus/ enterovirus (28.3%) were most commonly detected. A total of 114 (84.4%) children and 266 (87.8%) adults with respiratory viruses were treated with antibiotics. Among children, neutrophil percentage (median 63.6% vs 47.6%, p = 0.04) was positively associated with antibiotic prescription. Among adults, headache (60.6% vs 35.1%, p = 0.003), crepitations/crackles (55.3% vs 21.6%, p<0.001), rhonchi/wheezing (42.9% vs 18.9%, p = 0.005), and chest x-ray opacities (27.4% vs 8.1%, p = 0.01) were associated with antibiotic prescription. Access to CRP and procalcitonin test results could have potentially decreased inappropriate antibiotic prescription in this study by 89.5% and 83.3%, respectively. Conclusions: High proportions of viral detection and antibiotic prescription were observed among a large inpatient cohort with LRTI. Increased access to point-of-care biomarker testing may improve antimicrobial prescription. [ABSTRACT FROM AUTHOR]

We report results from a systematic analysis of thermal expansion of plasma-exposed tungsten based on molecular-dynamics simulations using models of tungsten with distributions of helium (He) bubbles in the tungsten matrix. We distinguish between two approaches of filling the bubbles with He, where the amount of He in the bubble can or cannot vary with temperature. In the former case, the thermal expansion coefficient decreases monotonically with the porosity and He content of the tungsten matrix, while in the latter case, the thermal expansivity increases monotonically with increasing porosity and He content. The latter condition, where the He content in the bubble is determined at the implantation temperature and remains constant with varying temperature in the tungsten matrix, is consistent with He species transport in tungsten used as a plasma-facing component (PFC) in nuclear fusion reactors and implies the development of biaxial compressive thermal strains in the PFC material that contribute to accelerating the growth of a nanostructure on PFC tungsten surfaces. Our analysis advances the fundamental understanding of thermal expansion in PFC tungsten and contributes to the development of a thermophysical property database for properly incorporating effects of realistic heat loads into modeling the dynamical response of PFC tungsten under fusion reactor operating conditions. [ABSTRACT FROM AUTHOR]

Cardiac arrest is a sudden loss of heart function with serious consequences. In developing countries, healthcare professionals use clinical documentation to track patient information. These data are used to predict the development of cardiac arrest. We published a dataset through open access to advance the research domain. While using this dataset, our work revolved around generating and utilizing synthetic data by harnessing the potential of synthetic data vaults. We conducted a series of experiments by employing state-of-the-art machine-learning techniques. These experiments aimed to assess the performance of our developed predictive model in identifying the likelihood of developing cardiac arrest. This approach was effective in identifying the risk of cardiac arrest in in-patients, even in the absence of electronic medical recording systems. The study evaluated 112 patients who had been transferred from the emergency treatment unit to the cardiac medical ward. The developed model achieved 96% accuracy in predicting the risk of developing cardiac arrest. In conclusion, our study showcased the potential of leveraging clinical documentation and synthetic data to create robust predictive models for cardiac arrest. The outcome of this effort could provide valuable insights and tools for healthcare professionals to preemptively address this critical medical condition. [ABSTRACT FROM AUTHOR]

In this cross-sectional descriptive study conducted in the Ratnapura district, Sri Lanka, we assessed the affordability of oral pediatric anti-infective medicines (OPAIMs). Using a modified WHO/HAI medicinal price methodology, we examined the availability, median price ratios (MPRs), mean percentage difference, and affordability of the standard treatment of the originator brand (OB) and lowest-priced generic (LPG) OPAIMs in 30 private and 2 state-owned pharmacies. The study revealed disparities in availability, with only 50% of private pharmacies offering all 11 medicinal drugs in their generic form. The MPRs of OPAIMs for OB and LPG varied, with three drugs exceeding the financially acceptable MPR of 2 (albendazole, amoxicillin, and erythromycin). The standard treatment with LPGs costs between 0.17 and 0.85 and between 0.06 and 0.28 days' wages for the lowest daily salary of the private sector and unskilled public employees, respectively. We identified erythromycin and albendazole as having less than 50% availability in their generic form in private pharmacies. To address these findings, we recommend frequent pricing revisions based on exchange rates and associated costs, coupled with the establishment of a transparent scientific criterion to subsidize essential medicines deemed "unaffordable." Failure to implement such measures amidst economic crises may adversely impact financial access to essential medications. [ABSTRACT FROM AUTHOR]

Fire spread scenarios associated with concealed cavity spaces have been relatively less discussed. The variation in studies with respect to geometry, influential parameters, and protection strategies has been an obstacle to deriving more generalized solutions in terms of cavity fire in buildings. A systematic literature review was conducted following the PRISMA method to identify the conclusive fire behaviour, safety risks, and protection strategies to enable future researchers to address cavity fire scenarios effectively, avoiding catastrophic disasters. This study identified that relative to open-fire scenarios, cavity fires could result in up to 10 times higher flame spread, up to 14 times higher heat exposure, and temperature conditions 13 times higher. Increased toxicity and smoke velocity are also found with cavity fires. Fire protection strategies and their efficiency were identified for a range of cavity geometries. Altogether, cavity spaces, especially narrow ones, cannot be neglected during fire safety, and proper risk identification is required to ensure the safety of the buildings and the occupants in a fire scenario. [ABSTRACT FROM AUTHOR]

Gaussian coherent state continuous variable quantum key distribution has gained interest owing to its security and compatibility with classical coherent optical fibre networks. For successful system deployment it is necessary to implement practical high speed systems which distil keys efficiently. Here, we demonstrate a Gaussian coherent state continuous variable quantum key distribution system at a 50 MHz symbol rate. Unlike most demonstrations to date which measure excess noise and infer key rates from this, we record signals in real time and distil keys. We also demonstrate, for the first time, slice reconciliation with optimised guard bands to maximise achievable secret key rates. Using this optimisation with multilevel slicing, a record 5 Mb/s secret key rate after a transmission distance of 25 km is achieved. This is a significant improvement on the 3 Mb/s secret key rate which is achieved with single level optimised slice reconciliation. [ABSTRACT FROM AUTHOR]

Objective: Data from South Asia indicate that for 15%–20% of suicide attempts, pesticides are purchased from shops; otherwise, pesticides are obtained from an individual's house or nearby environment. We aimed to investigate the difference between individuals who directly purchase pesticides from shops for suicide attempts and suicide deaths versus those related to accessing the pesticides from an individual's house or nearby environment. Methods: We conducted two comparative studies in rural Sri Lanka: (1) non‐fatal shop cases (n = 50) were survivors of self‐poisoning with pesticides who ingested the pesticides after purchasing them from a shop; non‐fatal domestic cases (n = 192) were survivors who accessed pesticides from their house or nearby environment. (2) fatal shop cases (n = 50) were individuals who died after ingesting pesticides they purchased for the act; fatal domestic cases (n = 102) were patients who died after ingesting pesticides they accessed at house or nearby environment. Logistic regression analysis was used to assess the characteristics which distinguished between the shop and domestic cases. Results: Data indicate that 20.7% and 32.9% of individuals who used pesticides for suicide attempts and suicide deaths had purchased them from shops, respectively. Being a non‐farmer was the main distinguishing characteristic of shop cases: adjusted odds ratios (AOR) 8.9, 95% confidence intervals (CI) 3.2–24.4 for non‐fatal shop cases, and AOR 4.0, 95% CI 1.5–10.6 for fatal shop cases. Non‐fatal shop cases also had higher suicide intent (AOR 3.0, CI 1.0–8.9), and ingesting an insecticide (AOR 4.8, CI 1.8–1.0–8.9) than non‐fatal domestic cases. Conclusion: A high suicide intent of individuals who purchase pesticides for the event explains the high proportion of such fatal cases. Such high suicide intent makes the prevention implications difficult to spell out for those individuals who purchase pesticides for self‐poisoning. However, our findings are valuable for clinicians to assess pesticide poisoning cases in hospitals. [ABSTRACT FROM AUTHOR]

Gastrochilus flabelliformis (Blatt. & McCann) C.J.Saldanha is added to the orchid flora of Sri Lanka from the lowland intermediate zone and G. obliquus (Lindl.) Kuntze is omitted from the list. A detailed taxonomic description, color plates, and line drawings with habitat ecology and distribution are provided for the former. Taxonomic notes on the identity of true Gastrochilus dasypogon (Sm.) Kuntze are also discussed in this paper, resulting in the merger of G. acutifolius (≡S. acutifolium), G. denticulatus (≡S. denticulatum), and G. dentatus (≡S. dentatum) under the synonymy of the former. [ABSTRACT FROM AUTHOR]

The apparel and textile industry is the backbone of the Sri Lankan economy, contributing significantly to the country's gross domestic product (GDP). The coronavirus (COVID-19) pandemic, which also triggered the ongoing economic crisis in Sri Lanka, has a profound effect on the organizational performance of apparel sector firms in Sri Lanka. In this context, the study examines the impact of multi-dimensional corporate sustainability practices on organizational performance in the said sector. The study employed the partial least squares structural equation modelling (PLS-SEM) technique for analysing and testing the hypothesis of the study while using Smart PLS 4.0 software as the analysis tool. Relevant data were collected through a questionnaire from 300 apparel firms registered with the Board of Investment of Sri Lanka (BOI). The study results indicated that "economic vigour," "ethical practices," and "social equity" have a significant impact on organizational performance, while "corporate governance" and "environmental performance" have an insignificant impact. Unique discoveries from this study would be useful to prosper organizational performance and formulate novel sustainable future strategies not limited to the garment industry even during harsh economic conditions. [ABSTRACT FROM AUTHOR]

Mental stress is found to be strongly connected with human cognition and wellbeing. As the complexities of human life increase, the effects of mental stress have impacted human health and cognitive performance across the globe. This highlights the need for effective non-invasive stress detection methods. In this work, we introduce a novel, artificial spiking neural network model called Online Neuroplasticity Spiking Neural Network (O-NSNN) that utilizes a repertoire of learning concepts inspired by the brain to classify mental stress using Electroencephalogram (EEG) data. These models are personalized and tested on EEG data recorded during sessions in which participants listen to different types of audio comments designed to induce acute stress. Our O-NSNN models learn on the fly producing an average accuracy of 90.76% (σ = 2.09) when classifying EEG signals of brain states associated with these audio comments. The brain-inspired nature of the individual models makes them robust and efficient and has the potential to be integrated into wearable technology. Furthermore, this article presents an exploratory analysis of trained O-NSNNs to discover links between perceived and acute mental stress. The O-NSNN algorithm proved to be better for personalized stress recognition in terms of accuracy, efficiency, and model interpretability. [ABSTRACT FROM AUTHOR]

Background: Caesarean section (CS) is associated with numerous complications that lead to the delayed return to functional activities that have a negative influence on the post-natal quality of life (QOL). It is evident that providing regular evidence-based physiotherapy training and education prior to elective CS helps to enhance the post-natal QOL by improving physical, mental, social, and general well-being. The purpose of this study was to examine the effectiveness of physiotherapy training and education prior to elective CS on post-natal QOL. Methods: This single-blind parallel randomized controlled study was carried out at De Soysa Hospital for Women (DSHW), Colombo. The study enrolled 54 women who were scheduled to undergo elective CS. The intervention group (n = 27) of women received physiotherapy training and education, while the control group (n = 27) received standard nursing care. In addition to the primary outcome measures, post-natal QOL was measured. The results were examined using descriptive statistics and the independent samples t-test in IBM SPSS 20. Results: The intervention group showed a higher post-natal QOL for the domains of physical function, role limitation due to physical health, energy/fatigue, and pain than the control group (p < 0.05). Conclusion: Physiotherapy training and education prior to elective CS play a pivotal role in improving the physical health-related domains of QOL following CS. Trial Registration: The Sri Lanka Clinical Trials Registry (https://www.slctr.lk). Registration number: SLCTR/2019/029-APPL/2019/028; Registration date: 6th of September 2019. [ABSTRACT FROM AUTHOR]

We report a systematic energetic analysis of helium-related defect interactions that mediate helium (He) segregation on surfaces of plasma-exposed tungsten at different levels of He ion implantation. We focus on high He fluences that increase the He content in the plasma-exposed material well beyond the dilute limit of He concentration and employ atomic configurations generated by large-scale molecular dynamics simulations of He-implanted tungsten. We perform systematic molecular statics computations of cluster–defect interaction energetics in the highly defect-rich near-surface region of plasma-exposed tungsten for small mobile helium clusters as a function of the clusters’ distances from the surface. In this region, mobile clusters are also subjected to the stress fields generated by defects such as helium bubbles and other clusters, which govern cluster–defect interactions in addition to the cluster–surface interaction. Based on systematic investigation of individual cluster–defect interactions, we develop a mathematical framework to describe the interaction energy landscapes consisting of elastic interaction potential perturbations to finite-width square-well potentials, where the potential well accounts for cluster trapping by the defect at close range and subsequent coalescence and the perturbation potential is parameterized according to elastic inclusion theory. Superposition of all the relevant interaction potentials provides a comprehensive description of the interaction energy landscape that would be experienced by a small mobile cluster along its migration path toward the plasma-exposed surface at high He fluences. Such descriptions are particularly important for developing atomistically informed, hierarchical multi-scale models of helium cluster dynamics in plasma-facing materials. [ABSTRACT FROM AUTHOR]

Recent advancements in space technology and reduced launching cost led companies, defence and government organisations to turn their attention to low Earth orbit (LEO) and very low Earth orbit (VLEO) satellites, for they offer significant advantages over other types of spacecraft and present an attractive solution for observation, communication and other tasks. However, keeping satellites in LEO and VLEO presents a unique set of challenges, in addition to those typically associated with exposure to space environment such as damage from space debris, thermal fluctuations, radiation and thermal management in vacuum. The structural and functional elements of LEO and especially VLEO satellites are significantly affected by residual atmosphere and, in particular, atomic oxygen (AO). At VLEO, the remaining atmosphere is dense enough to create significant drag and quicky de-orbit satellites; thus, thrusters are needed to keep them on a stable orbit. Atomic oxygen-induced material erosion is another key challenge to overcome during the design phase of LEO and VLEO spacecraft. This review covered the corrosion interactions between the satellites and the low orbit environment, and how it can be minimised through the use of carbon-based nanomaterials and their composites. The review also discussed key mechanisms and challenges underpinning material design and fabrication, and it outlined the current research in this area. [ABSTRACT FROM AUTHOR]

Making internet-of-things (IoT)-based applications context-aware demands large amounts of raw data to be collected, interpreted, stored, and reused or repurposed if needed from many domains and applications. Context is transient but interpreted data can be distinguished from IoT data in many aspects. Managing context in cache is a novel area of research that has been given very little attention. Performance metric-driven adaptive context caching (ACOCA) can have a profound impact on the performance and cost efficiency of context-management platforms (CMPs) when responding to context queries in realtime. Our paper proposes an ACOCA mechanism to maximize both the cost and performance efficiency of a CMP in near realtime. Our novel mechanism encompasses the entire context-management life cycle. This, in turn, distinctively addresses the problems of efficiently selecting context for caching and managing the additional costs of context management in the cache. We demonstrate that our mechanism results in long-term efficiencies for the CMP that have not been observed in any previous study. The mechanism employs a novel, scalable, and selective context-caching agent implemented using the twin delayed deep deterministic policy gradient method. It further incorporates an adaptive context-refresh switching policy, a time-aware eviction policy, and a latent caching decision management policy. We point out in our findings that the additional complexity of adaptation introduced to the CMP through ACOCA is significantly justified, considering the cost and performance gains achieved. Our algorithm is evaluated using a real-world inspired heterogeneous context-query load and a data set based on parking-related traffic in Melbourne, Australia. This paper presents and benchmarks the proposed scheme against traditional and context-aware caching policies. We demonstrate that ACOCA outperforms the benchmarks in both cost and performance efficiency, i.e., up to 68.6%, 84.7%, and 67% more cost efficient compared to traditional data caching policies to cache context, redirector mode, and context-aware adaptive data caching under real-world-like circumstances. [ABSTRACT FROM AUTHOR]

A study was conducted to test the validity of four different mathematical models imbedded in CropWat software, which is widely used to estimate effective rainfall. The model values were statistically compared with the actual effective rainfall values measured by a field infiltrometer experiment. The study was conducted in a sugarcane land–use system under the tropical climate of Sevanagala, Sri Lanka, during the 2018 to 2019 growing season. The measured effective rainfall in the Sevanagala sugarcane growing area during the 12-month period was 1,068 mm , around 70% of the total rainfall of 1,517 mm. It was also found that 9% of the rainfall received at the top of the canopy was lost due to canopy interception. The results demonstrated the applicability of the mathematical models embedded in CropWat software to estimate the effective rainfall for sugarcane land–use systems in Sevanagala. Furthermore, the empirical regression formula embedded in CropWat software appeared to be the best-fit model for the estimation of effective rainfall because it had the highest index of agreement value, with an accuracy greater than 96%. The current findings can be used to accurately estimate the effective rainfall, which is the actual contribution of rainfall to be used by the sugarcane crop. The results obtains through this process can facilitate managers to prepare plans to reduce irrigation supply without affecting potential crop yields. Furthermore, findings will be useful to technical staff working in the fields of irrigation and water management. The information will be equally helpful to national-level regulatory agencies to prepare feasibility studies and plan future irrigation projects within the country. Irrigation agronomists will also benefit from the paper content because it will help them to handle farm-level operations and irrigation cost-reduction programs. Furthermore, the contents of this article will also be of interest to students and researchers working on hydrological modeling, crop simulation modeling, drought assessment, and empowering sustainable agriculture programs. [ABSTRACT FROM AUTHOR]

Background: This study examined changes in population-level co-use of cannabis and alcohol before and 12 months after nonmedical cannabis legalization in Canada, relative to the United States that had previously legalized and not legalized (US legal and illegal states, respectively). Methods: Data are from waves 1 and 2 of the International Cannabis Policy Study, collected in 2018 (before) and 2019 (12 months after legalization in Canada). Respondents aged 16–65 years from Canada (n = 25,313) and US legal (n = 25,189) and US illegal (n = 19,626) states completed an online survey. Changes in co-use between 2018 and 2019 in US legal and illegal states compared to those in Canada were assessed using multinomial logistic regression. Results: Descriptive analyses show increases in cannabis use and monthly or more frequent (MMF) co-use between 2018 and 2019 in all jurisdictions. Compared to no MMF use of cannabis or alcohol, there was no evidence suggesting differences in changes in MMF co-use in US legal or illegal states relative to Canada. However, respondents in US legal states had 33% higher odds of MMF alcohol-only use (OR = 1.33, 99% CI: 1.12, 1.57) compared to no MMF use relative to Canada. Conclusions: Increases in co-use were observed between 2018 and 2019 in all jurisdictions regardless of the legal status of cannabis. These shifts were largely due to increases in cannabis use across the population, including those that use alcohol, and may indicate changing societal norms toward cannabis generally. As the cannabis legalization transition in Canada matures, evaluation over the longer term will improve understanding of the influence of cannabis liberalization on co-use. [ABSTRACT FROM AUTHOR]

Mica, a commonly occurring mineral, has significant potential for various applications due to its unique structure and properties. However, due to its non-Van Der Waals bonded structure, it is difficult to exfoliate mica into ultrathin nanosheets. In this work, we report a rapid solvothermal microwave synthesis of 2D mica with short reaction time and energy conservation. The resulting exfoliated 2D mica nanosheets (eMica nanosheets) were characterized by various techniques, and their ability to capture CO2 was tested by thermogravimetric analysis (TGA). Our results showed an 87% increase in CO2 adsorption capacity with eMica nanosheets compared to conventional mica. Further characterization by Fourier-transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS), as well as first-principles calculations, showed that the high specific surface area and deposited K2CO3 layer contribute to the increased CO2 adsorption on the mica nanosheets. These results speak to the potential of high-quality eMica nanosheets and efficient synthesis processes to open new avenues for new physical properties of 2D materials and the development of CO2 capture technologies. [ABSTRACT FROM AUTHOR]

This research captures the complex interaction between land use and transportation in an urban context. Even though several land use–transport interaction models at both micro and macro levels are available, there is a necessity to address the intermediate-level model with acceptable accuracy and without intensive data requirement. Further, consideration of road networks and land use in the traffic analysis zone demarcation is a significant requirement to enhance accuracy in estimating passenger flow distribution. This research provides a methodology to identify modified traffic analysis zones (MTAZs) based on traffic flow distribution and to redistribute macrolevel trip origins and destinations into MTAZs considering the road network and land-use character of the area. The core objective of the research is to develop a methodology to demonstrate the relationship between trip production/attraction and land use, to propose a method to redistribute macrolevel origin-destination data into MTAZs based on the identified relationship and to propose a method to assign estimated traffic flows to the road links. The methodology intends to guide the land use and transportation decision making. The methodology is developed based on both spatial and statistical analysis utilizing software and tools, such as ArcMAP, SPSS, and MS Excel. A case study covering the Colombo Municipal Council area, which is located within Colombo District, Western Province, Sri Lanka, is presented to demonstrate the application of the proposed methodology. According to the validation results, the proposed methodology has increased the accuracy of passenger flow estimation compared with the administrative zone-based conventional passenger flow estimation methodologies. [ABSTRACT FROM AUTHOR]

The present study was conducted to understand the changes in gonads and hematological parameters in climbing perch (Anabas testudineus) exposed to synthetic estrogen and progestogen [mixture of ethinylestradiol (EE2) and desogestrel (DES)]. Climbing perch were exposed to four different concentrations of EE2/DES mixtures, viz. 0 ng of EE2 and DES/L (T0), 3 ng EE2 and 15 ng DES/L (T3), 30 ng EE2 and 150 ng DES/L (T30), and 300 ng EE2 and 1500 ng DES/L (T300) for 60 days. On days 45 and 60, samples were taken to assess changes in somatic indexes, gonad histology, and hematological parameters. The gonadosomatic index (GSI) increased in both females and males with increasing concentrations of estrogen mixtures except for T30 females, which was the lowest among all\four treatments. The hepatosomatic index (HSI) was observed to be increased in males as estrogen content increased. However, compared to fish at T0, HSI in female individuals did not vary in T30 fish, where the value was the highest among all the treatments. On day 45, histological observations showed no feminization or intersexuality but several germ-cell deformities in the ovary (adhesion, degenerated oocyte wall, degenerated granulose layer, increased interfollicular space, atretic follicle, and cytoplasmic clumping) and testes (increased interstitial area, focal loss of spermatocyte, dilation of the lumen, breakage of tubular epithelium, and elongated seminiferous tubule) were observed in fish exposed to EE2 and DES. Fish reared at T30 had lower RBC count, hemoglobin (Hb), glucose, and hematocrit levels. On day 60, fish reared at T30 had the highest Hb content compared to fish raised in other treatment conditions. WBC was progressively higher with increasing EE2/DES concentrations. Significant erythrocyte cytoplasmic abnormalities and erythrocyte nuclear abnormalities were observed in fish exposed to higher EE2/DES concentrations. The present study provides insights into the adverse impacts of synthetic estrogens sourced from human contraceptive pills on fish physiology. [ABSTRACT FROM AUTHOR]

Visual impairment is occasionally observed in multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Although uveitis and optic neuritis have been reported in MS and EAE, the precise mechanisms underlying the pathogenesis of these visual impairments remain poorly understood. This study aims to identify differentially expressed genes (DEGs) in the retinas of mice with EAE to identify genes that may be implicated in EAE-induced visual impairment. Fourteen adult mice were injected with myelin oligodendrocyte glycoprotein35–55 to induce the EAE model. Transcriptomes of retinas with EAE were analyzed by RNA-sequencing. Gene expression analysis revealed 347 DEGs in the retinas of mice with EAE: 345 were upregulated, and 2 were downregulated (adjusted p-value < 0.05 and absolute log2 fold change > 1). Gene ontology (GO) analysis showed that the upregulated genes in the retinas of mice with EAE were primarily related to immune responses, responses to external biotic stimuli, defense responses, and leukocyte-mediated immunity in the GO biological process. The expression of six upregulated hub genes (c1qb, ctss, itgam, itgb2, syk, and tyrobp) from the STRING analysis and the two significantly downregulated DEGs (hapln1 and ndst4) were validated by reverse transcription-quantitative polymerase chain reaction. In addition, gene set enrichment analysis showed that the negatively enriched gene sets in EAE-affected retinas were associated with the neuronal system and phototransduction cascade. This study provides novel molecular evidence for visual impairments in EAE and indicates directions for further research to elucidate the mechanisms of these visual impairments in MS. [ABSTRACT FROM AUTHOR]

Background: End-of-life care involves a multitude of functions delivered by a team of healthcare professionals. Family caregivers get involved in every aspect of the palliative care journey. Meeting the needs of ethnically diverse patients can be a daunting task for Western-trained healthcare professionals. Family and professional caregivers need to have a mutual understanding of perspectives and expectations to integrate family caregivers into end-of-life care. The South Asian population in Canada is fast growing, and very little is known about their understanding and expectations of end-of-life care. Methods: The purpose is to provide research-based knowledge on discordances and concordances of encounters and perceptions of end-of-life care delivery between South Asian family caregivers and palliative care health professionals. Individual interviews were conducted among seven palliative care professionals, in a tertiary care center, and seven South Asian family caregivers who have provided care, in the same inpatient center, for the same period. The constant comparison, a component of the grounded theory approach, was employed to compare the two types of caregivers' perspectives that emerged in the qualitative data. Findings: The family caregivers were divided in their perception based on death denial and acceptance. The findings weaved the discordances and concordances of meaning assigned to palliative care to the three themes that emerged: the role of the family caregiver, communication needs and challenges, and barriers to the family caregiver participation in decision-making. The discordance between professionals and family caregivers arose in the death-denial group and concorded with the death-accepted group. The findings revealed a consequence of the survival optimistic bias, as creating dissatisfaction toward the end-of-life care delivery system when the palliative care professionals prognosticate imminent end-of-life. Conclusion: The family caregivers' interactions and encounters were shaped by their acceptance or denial of the death of their family member in care. Gaining conceptual clarity on the meaning of palliative care and providing education on the process of end-of-life care delivery are crucial to integrating ethnically diverse family caregivers into the decision-making process. [ABSTRACT FROM AUTHOR]

The growing need of utilizing unstructured knowledge embedded in open-domain natural language text into machine-processable forms requires the induction of hardly extracted structured knowledge into knowledge bases which makes the Semantic Web vision a reality. In this context, ontologies, and ontological knowledge (triples) plays a vital role. This research introduces two novel concepts named Directed Collocation (DC) and Joined Directed Collocation (JDC) along with a methodical application of them to infer new ontological knowledge. Introduced Quality-Threshold-Value (QTV) parameter improves the quality of the inferred ontological knowledge. Having set a moderate value (3) for QTV, this approach inferred 95,491 new ontological knowledge from 43,100 triples of open domain Sri Lankan English news corpus. Indeed, the outcome was approximately doubled in size as the source corpus. Some inferred ontological knowledge was identical with the original corpus content, which evidences the accuracy of this approach. The remaining were validated using inter-rater agreement method (high reliability) and out of which around 56% were estimated as effective. The inferred outcome which is in the triple format may use in any knowledge base. The proposed approach is domain independent. Thus, helps to construct/extend ontologies for any domain with the help of less or no human specialists. [ABSTRACT FROM AUTHOR]

Fungal involvement in the biodeterioration of low‐density polyethylene (LDPE) has received great attention in recent years. Among diverse groups of fungi, endolichenic fungi (ELF) are adapted to thrive in resource‐limited conditions. The present study was designed to investigate the potential of mangrove‐associated ELF, in the biodeterioration of LDPE and to quantify key‐depolymerizing enzymes. A total of 31 ELF species, isolated from 22 lichens of mangrove ecosystems in Negombo lagoon, Sri Lanka were identified using DNA barcoding techniques. ELF were inoculated into a mineral salt medium, containing LDPE strips and incubated at 28 ± 2°C, for 21 days, under laboratory conditions. After incubation, biodeterioration was monitored based on percent reductions in weights and tensile properties, increments in the degree of water absorption, changes in peaks of infrared spectra and surface erosions using scanning electron microscopy. Out of 31 species, Chaetomium globosum, Daldinia eschscholtzii, Neofusicoccum occulatum, Phanerochaete chrysosporium, Schizophyllum commune and Xylaria feejeensis showed significant changes. Production of depolymerizing enzymes by these species was assayed qualitatively using plate‐based methods and quantitatively by mass‐level enzyme production. Among them, Phanerochaete chrysosporium showed the highest enzyme activities as (9·69 ± 0·04) × 10−3, (1·96 ± 0·01) × 10−3, (5·73 ± 0·03) × 10−3, (0·88 ± 0·01), (0·64 ± 0·06), (1·43 ± 0·01) U ml−1 for laccase, lignin peroxidase, manganese peroxidase, amylase, lipase and esterase, respectively. [ABSTRACT FROM AUTHOR]

Experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS), approximates the key histopathological, clinical, and immunological features of MS. Hippocampal dysfunction in MS and EAE causes varying degrees of cognitive and emotional impairments and synaptic abnormalities. However, the molecular alterations underlying hippocampal dysfunctions in MS and EAE are still under investigation. The purpose of this study was to identify differentially expressed genes (DEGs) in the hippocampus of mice with EAE in order to ascertain potential genes associated with hippocampal dysfunction. Gene expression in the hippocampus was analyzed by RNA-sequencing and validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Gene expression analysis revealed 1202 DEGs; 1023 were upregulated and 179 were downregulated in the hippocampus of mice with EAE (p-value < 0.05 and fold change >1.5). Gene ontology (GO) analysis showed that the upregulated genes in the hippocampi of mice with EAE were associated with immune system processes, defense responses, immune responses, and regulation of immune responses, whereas the downregulated genes were related to learning or memory, behavior, and nervous system processes in the GO biological process. The expressions of hub genes from the search tool for the retrieval of interacting genes/proteins (STRING) analysis were validated by RT-qPCR. Additionally, gene set enrichment analysis showed that the upregulated genes in the hippocampus were associated with inflammatory responses: interferon-γ responses, allograft rejection, interferon-α responses, IL6_JAK_STAT3 signaling, inflammatory responses, complement, IL2_STAT5 signaling, TNF-α signaling via NF-κB, and apoptosis, whereas the downregulated genes were related to synaptic plasticity, dendritic development, and development of dendritic spine. This study characterized the transcriptome pattern in the hippocampi of mice with EAE and signaling pathways underpinning hippocampal dysfunction. However, further investigation is needed to determine the applicability of these findings from this rodent model to patients with MS. Collectively, these results indicate directions for further research to understand the mechanisms behind hippocampal dysfunction in EAE. [ABSTRACT FROM AUTHOR]

Histone deacetylase (HDAC) is an enzyme that deacetylates the histone protein by removing the acetyl group from the lysine residues. The overexpression of the HDAC enzyme alters the gene expressions and causes cancer development in the human body. The inhibition of HDAC is an excellent therapeutic way in current cancer therapy. In this regard, various inhibitors were selected, and the inhibitory potential of these inhibitors was examined by molecular dynamics (MD) simulation followed by trajectory analysis and binding energy calculations. The selected clinical trial II and III phase inhibitors are TSA (trichostatin-A), TFMK (trifluoromethyl-ketone-9,9,9-trifluoro-8-oxo-N-phenylnonanamide), AKA (alpha-ketoamide-N-cyclohexyl-N-methyl-2-oxononanediamide), ITF2357 (givinostat), MS275 (entinostat), CI994 (tacedinaline), and SAHA (suberoylanilide hydroxamic acid). This computational study examines the atomic level description of the drug binding site on the HDLP enzyme and investigates the interaction of the HDAC inhibitors with the amino acid residues attached to the active site of the histone deacetylase-like protein (HDLP). Root-mean-square deviation, radius of gyration, hydrogen bond analysis, MM-PBSA, linear interaction energy (LIE), and semi-LIE calculations have revealed that the HDLP enzyme is more stabilized when bound to TSA, ITF2357, and reference inhibitor SAHA. It was observed that the hydroxamic acid family inhibitors have more potent in inhibiting the HDLP enzyme than the benzamide and ketone families. The inhibitory efficacy of TSA and ITF2357 is much similar to that of SAHA. Therefore, these HDAC inhibitors have the potential to be used in future clinical practices for cancer-related treatments. The knowledge gathered from this study could also lead to discovering new HDAC inhibitors for clinical research. [ABSTRACT FROM AUTHOR]

Purpose: Parkinson disease (PD) is a progressive neurodegenerative disorder in which dopaminergic (DAergic) systems are destroyed (particularly in the nigrostriatal system), causing both motor and nonmotor symptoms. Hippocampal neuroplasticity is altered in PD animal models, resulting in nonmotor dysfunctions. However, little is known about the precise mechanism underlying the hippocampal dysfunctions in PD. Methods: Striatal 6-hydroxydopamine (6-OHDA) infusions were performed unilaterally in adult Sprague Dawley rats. Both motor and nonmotor symptoms alongside the expression of tyrosine hydroxylase (TH) in the substantia nigra and striatum were confirmed in 6-OHDA-lesioned rats. The neuronal architecture in the hippocampus was analyzed by Golgi staining. Results: During the 7-8 weeks after infusion, the 6-OHDA-lesioned rats exhibited motor and nonmotor dysfunctions (especially anxiety/depression-like behaviors). Rats with unilateral 6-OHDA infusion displayed reduced TH+ immunoreactivity in the ipsilateral nigrostriatal pathway of the brain. Golgi staining revealed that striatal 6-OHDA infusion significantly decreased the dendritic complexity (i.e., number of crossing dendrites, total dendritic length, and branch points) in the ipsilateral hippocampal conus ammonis 1 (CA1) apical/basal and dentate gyrus (DG) subregions. Additionally, the dendritic spine density and morphology were significantly altered in the CA1 apical/basal and DG subregions following striatal 6-OHDA infusion. However, alteration of microglial and astrocytic distributions did not occur in the hippocampus following striatal 6-OHDA infusion. Conclusions: The present study provides anatomical evidence that the structural plasticity in the hippocampus is altered in the late phase following striatal 6-OHDA infusion in rats, possibly as a result of the prolonged suppression of the DAergic system, and independent of neuroinflammation. [ABSTRACT FROM AUTHOR]

Epigenetics regulates gene expression through post-translational modification of protein complexes accompanying DNA. The most significant modification in histone protein is deacetylation, regulated by HDAC enzyme. This study focuses on the HDAC inhibitors' effect on the HDLP enzyme's stability. Ab initio quantum mechanics (QM), Quantum mechanics/molecular mechanics (QM/MM), and Molecular dynamics (MD) analyses were executed to investigate the changes that occur in the HDLP enzyme due to inhibitor binding. LBH589, PCI24781, PXD101, LAQ824, SB939, and RAS2410 were selected as inhibitors, and SAHA was used as a reference inhibitor. The crystal structure of the HDLP enzyme was obtained from the Protein Data Bank, and the inhibitor structures were optimized using G09W. AutoDock-Vina was used to perform docking, and the resultant complex was used to perform MD simulation. The QM/MM two-layer binding energy studies revealed that the stabilities of HDLP-LBH589 and HDLP-PCI24781 are higher than the other studied complexes. Also, it was found that the pKa values of the inhibitors are correlated with their binding energy. Further, the trajectory analysis results showed that the complexes of HDLP with LBH589, PCI24781, and SAHA are comparatively more stable. Therefore, it is concluded that LBH589 and PCI24781 are excellent candidates to be studied as HDLP inhibitors. [ABSTRACT FROM AUTHOR]

Pathogenic bacteria utilize specialized macromolecular secretion systems to transport virulence factors across membrane(s) and manipulate their infected host. To date, 11 secretion systems have been identified, including the type IX secretion system (T9SS) associated with human, avian and farmed‐fish diseases. As a bacterial secretion system, the T9SS also facilitates gliding motility and the degradation of different macromolecules by the secretion of metabolic enzymes in nonpathogenic bacteria. PorX is a highly conserved protein that regulates the transcription of essential T9SS components and additionally mediates the function of T9SS via direct interaction with PorL, the rotary motor protein of the T9SS. PorX is also a member of a two‐component system regulatory cascade, where it serves as the response regulator that relays a signal transduced from a conserved sensor histidine kinase, PorY, to a designated sigma factor. Here, the recombinant expression and purification of PorX homologous proteins from the pathogenic bacterium Porphyromonas gingivalis and the nonpathogenic bacterium Flavobacterium johnsoniae are reported. A bioinformatical characterization of the different domains comprising the PorX protein is also provided, and the crystallization and X‐ray analysis of PorX from F. johnsoniae are reported. [ABSTRACT FROM AUTHOR]

Background: Pertussis is an infectious disease of the respiratory tract with a changing epidemiology. An increasing incidence has been found in the adult population with recurrent infections possibly related to changes in the current vaccine. Is there an association between pertussis infection, refractory cough and atypical gastro-oesophageal reflux (GORD)? Does this magnify and compound respiratory complications? Methods: Observational study which compares post-pertussis (n=103) with non-pertussis patients (n=105) with established GORD. Patients were assessed for laryngopharyngeal reflux and aspiration of refluxate by a novel scintigraphic study. Results: Both groups showed severe GORD in association with high rates of laryngopharyngeal reflux (LPR) and pulmonary aspiration and lung disease. High rates of hiatus hernia and clinical diagnosis of "atypical" asthma showed correlations with pulmonary aspiration. Conclusions: A high level of new onset LPR and lung aspiration has been shown in patients with chronic cough after recent pertussis infection by a novel scintigraphic technique with fused hybrid x-ray computed tomography (SPECT/CT). [ABSTRACT FROM AUTHOR]

Background: With overpopulation contributing to the depletion of planetary resources, the high rates of unintended pregnancies in India are a cause for concern. Despite the free supply of contraception options within India's national family planning initiatives, women are generally offered hormonal options as temporary spacing methods. However, female condoms, a much neglected but potent woman initiated, non-hormonal multipurpose prevention device, are yet to be considered for inclusion in India's contraceptive cafeteria. Thus, we aim to examine the place of female condoms among the contraceptive options, by analysing the perceptions of key stakeholders regarding its advantages and disadvantages, along with their opinions on how female condoms should be promoted.Methods: We used purposive sampling to recruit and interview potential users and dispensers of the female condom. The interview participants visited or worked at family planning clinics in Pune at Smt. Kashibai Navale Medical College and General Hospital (SKNMC-GH), its urban and rural outreach clinics, and at Saheli (a non-governmental organisation for female sex workers). We conducted semi-structured interviews and coded our data inductively.Results: We interviewed 5 rural women, 20 urban women (including 10 female sex workers), 5 male partners of female sex workers, and 5 family planning healthcare providers. Nearly half (12/25) of the women we interviewed, said that they were eager to use female condoms in the future. Many participants perceived female condoms to be an instrument to empower women to be in control of their sexual and reproductive lives (15/35), and that it provided user comfort and confidence (4/35). Their perceived disadvantages are that they are relatively more expensive (6/35), users have limited experience (9/35), and women who buy or use them may be stigmatised and feel embarrassed (4/35). Yet, nearly three-quarters of potential users (21/30) and most healthcare providers (4/5), were confident that female condoms could become popular following extensive promotional campaigns, interventions to improve availability and access, and initiatives to enhance the knowledge of female users.Conclusions: Female condoms have garnered support from both users and dispensers and have the potential to be widely adopted in India if family planning initiatives which increase awareness, knowledge, and access are systematically undertaken as with other contraceptive options. [ABSTRACT FROM AUTHOR]

Increase in the variety of development in urban context has made it more complicated and complex for the users of public spaces. Absence of sufficient information to read the surrounding causes psychological anxiousness leading to perceived danger or discomfort for the urbanites. Consequently, perceived safety and comfort of the users is distinctively low in urban contexts, creating neglected and underused spaces. Complexity is one of the information processing variables as per Kaplan and Kaplan's informational model which helps users to comprehend the surrounding environment. The streetscape plays a vital role in the daily movement patterns within the urban cities and is the transition boundary between the public and private realms. Visual complexity of these streets is a result of different configurations of elements within the urban areas. This research is conducted to ascertain the relationship between visual complexity levels of the streets with the perceived safety and comfort of the users. Shannon Diversity Index (SDI) and Fractal dimension analysis were conducted with 48 SVIs (Street View Images) selected within 1km radius of Colpetty junction, Colombo Sri Lanka covering all the possible compositions found within the context. The visual index data extraction had identified ten major components within the selected 48 SVIs. 78 subjective ranking responses for perceived safety, comfort (preference) and perceived complexity were collected from snowball sampling. Findings of the study revealed that perceived safety levels and preference scores for the SVIs are related to the Shannon Diversity Index calculation in an inverted 'U' shape where the highest and lowest SDI values are related with low preference scores and low safety levels. The SVIs with medium SDI values are perceived as the safest and most preferred by the users of urban streets of Colombo Sri Lanka. The SDI and fractal dimension values were significantly correlated with the perceived complexity scores of the users. The results of this study can be accommodated in the planning and designing of urban streetscapes of tropical climates for sustainable and friendly urban expansions. [ABSTRACT FROM AUTHOR]

Chitosan is a well-studied biomaterial which has been widely used for environmental applications as an efficient natural polymer for the adsorption and removal of metal ions. Owing to its unique properties, chitosan shows good metal-binding behavior toward several different metal ions such as Cu2+, Zn2+, Cd2+, Ni2+, Co2+, and Ca2+. Chemical modifications with the introduction of functional groups have been carried out extensively and thereby producing various chitosan derivatives to increase the selectivity and adsorption capacity toward metal ions. The present work focuses on two such monofunctional derivatives, namely, carboxymethyl chitosan (CMC) and ethylenediaminetetraacetic acid chitosan (EDTA-CS) which have been recognized as excellent adsorbents for metal removal. The main objective of this study was to synthesize a new bifunctional chitosan derivative, namely, ethylenediaminetetraacetic acid–carboxymethyl chitosan (EDTA-CMC) by attaching both carboxymethyl and EDTA functional groups on the polymer backbone and thereby enhancing its metal-binding properties. The bifunctional derivative synthesis was conducted by combining the procedures of synthesis of CMC and EDTA-CS. Newly synthesized EDTA-CMC derivative was characterized by Fourier-transform infrared (FT-IR) spectroscopy, scanning electron microscope analysis, and thermogravimetric analysis. Adsorption properties of EDTA-CMC were investigated with Cu2+ ions which produced an adsorption capacity of 111.90 mg g−1 for 1000.0 mg/L and 12.20 mg g−1 for 10.00 mg/L Cu2+ solutions. The preliminary results revealed that EDTA-CMC is an effective adsorbent than CMC to remove Cu2+ in aqueous samples. The effects of pH, initial concentration, and mass of the adsorbent in the adsorption process were studied. Under the optimized parameters of an adsorbent dosage of 10.00 mg and pH 5.5, a comparable maximum adsorption capacity up to 112.44 mg g−1 was achieved with a 150.00 mg/L of Cu2+ solution. Furthermore, EDTA-CMC showed good adsorption performance even after five cycles of regeneration. [ABSTRACT FROM AUTHOR]

The provision of hallway healthcare is a growing concern within contemporary healthcare systems. Hallway healthcare or hallway medicine is defined as the use of unconventional spaces, such as hallways, to provide patient care in the acute care setting. Negative effects associated with the prevalence of hallway healthcare may hinder the healing trajectory of vulnerable and ageing populations. Nurses have an intimate role in the provision of care and can offer valuable insight for the advocacy of high quality and safe patient care. Moreover, hallway healthcare is associated with the development of suboptimal working conditions that have resulted in negative impacts on the nursing profession. The authors seek to better understand the occurrence of this phenomenon by exploring the development of healthcare policies in relation to the neoliberal tenets of, individualism, free market via deregulation and privatization, and decentralization. This article provides an analysis of the historical evolution of hallway healthcare and neoliberalism. Furthermore, the authors aim to explore and demonstrate how the COVID‐19 pandemic has shed light on the inefficiency of neoliberalism ideologies for healthcare. Based on the analysis, the authors shall provide suggestions for nurses and stakeholders to enact meaningful change in an international context. [ABSTRACT FROM AUTHOR]

The aim of this study was to characterise pulmonary aspiration of refluxate in patients with gastroesophageal reflux disease (GORD) and laryngopharyngeal reflux (LPR) by continuous pulse oximetry (SpO2) during the supine phase of a scintigraphic reflux study. Variables assessed for significance included age, hiatus hernia, frequency, amplitude of reflux and clearance of reflux from the oesophagus/pharynx. The patients included in this study had established GORD and LPR by clinical history. All patients underwent fused three‐ dimensional scintigraphic/ X‐ray computed tomography (CT) and simultaneous continuous pulse oximetry when supine for 30 minutes. A total of 265 patients (40.4% M, 59.6% F) were studied. Mean age of aspirators was 57.0 years and non‐aspirators was 53.5 years. Seven patients had baseline oxygen saturation <95%, with 6/7 showing aspiration by scintigraphy. The remainder had mean baseline saturation of 97.7%. Continuous SpO2 monitoring showed a significant fall in pulmonary aspirators after 20 min of supine acquisition with significant variability. Analysis revealed a cyclic event every 1.5 min in aspirators only. Panel regression analysis showed a significant effect of age, hiatus hernia, pulse rate and reflux frequency on the fall in SpO2. Pulmonary aspiration in patients with LPR and GORD is characterised by acute oxygen desaturation. Variables affecting oxygen desaturation were age, hiatus hernia, pulse rate and reflux frequency. A cyclic event was observed every 1.5 min in aspirators and may be due to reflex homeostatic mechanism attempting to correct perceived hypoxia. [ABSTRACT FROM AUTHOR]

While perovskite solar cell (PSC) efficiencies are soaring at a laboratory scale, these are most commonly achieved with evaporated gold electrodes, which would present a significant expense in large‐scale production. This can be remedied through the use of significantly cheaper carbon electrodes that, in contrast to metals, also do not migrate through the device. To this end, the present work investigates simple‐to‐prepare aluminum‐supported carbon electrodes derived from commercially available, inexpensive materials that can be applied onto various hole‐transporting materials and enable photovoltaic performances on par with those provided by gold electrodes. Successful integration of the new carbon‐based electrode into flexible devices produced by a roll‐to‐roll printing technology by both pressing and lamination is demonstrated. However, temperature cycling durability tests reveal that the use of carbon electrodes based on commercial pastes is hindered by incompatibility of adhesive additives with the key components of the PSCs under heating. Resolving this issue, tailor‐made graphite electrodes devoid of damaging additives are introduced, which improve the PSC stability under temperature cycling test protocol to the level provided by benchmark gold electrodes. The study highlights current challenges in developing laminated carbon electrodes in PSCs and proposes strategies toward the resolution thereof. [ABSTRACT FROM AUTHOR]

Ingestion of organophosphate (OP) pesticides is a common method of self-harm in developing countries. Apart from acute cholinergic effects, limited evidence implicates OP poisoning in long-term neurocognitive deficits. However, prospective neurophysiological evidence of long-term deficits is scarce in humans. We aimed to determine long-term cognitive changes of acute OP pesticide self-poisoning in a prospective follow-up study, using event-related potentials (ERPs), an electroencephalographic index of cognitive functioning. We recruited 203 patients (147 men) hospitalised with OP pesticide ingestion (OP group; all had significant erythrocyte cholinesterase inhibition) and 50 patients (23 men) with paracetamol overdose (control group) as a means of self-harm. We recorded their ERPs and behavioural performance in a selective attention task at three post-exposure time points: on discharge from hospital (around 14 days post-ingestion), 6 weeks and 6 months post-ingestion. We compared the reaction time and ERP components of the two groups at each time point, adjusting for sex, age, education and comorbid depression in multiple regression models. OP group had significantly slower reaction times than the control group on discharge and at 6 weeks, but not at 6 months. On discharge, the OP group also showed significantly prolonged latency of the parietal P3b component, signifying delayed attentional processing. P3b amplitudes were also significantly smaller in the OP group on discharge and at 6 months. Within the OP group, greater clinical severity of poisoning was associated with smaller P3b amplitudes. Early pre-attentive cortical processing (as indexed by N1 ERP component) showed no significant intergroup differences. Acute OP pesticide poisoning was associated with impaired behavioural and neurophysiological indices of selective attention. These deficits outlast the cholinergic phase of intoxication. The neurobehavioral impairment disappears over months, but neurophysiological deficits seem to last even after 6 months. This impairment could potentially compromise the performance and safety of patients for months following clinical recovery. [ABSTRACT FROM AUTHOR]

Bioassay-guided fractionation of the ethyl acetate extract of Phanerochaete sordida, an endolichenic fungus (ELF) isolated from the host lichen Bactrospora myriadea, collected from Negombo lagoon, Sri Lanka, led to the isolation of a bioactive compound. Following the identification of the fungus using morphological and DNA barcoding techniques, the pure compound was isolated using column chromatography, preparative TLC, and semipreparative HPLC. The structure elucidation was carried out using IR, HR-ESI-MS and 1H, 13C & 2D NMR spectroscopic methods. The in vitro bioassays conducted revealed that compound 1 has a high antioxidant activity with ABTS•+ (IC50 58.91 ± 1.35 μ M), moderate anti-inflammatory activity (IC50 254.79 ± 1.41 μ M), comparable antibacterial activity against the oral-bacterial strain Streptococcus mutans (MIC 898.79 μM and MLC 1797.58 μM), moderate tyrosinase inhibition (IC50 1713.69 ± 8.65 μ M), and moderate cytotoxicity against oral cancer (IC50 13.65 ± 0.02 μ M), in comparison with respective positive controls. The in silico experiments conducted for tyrosinase inhibition and cytotoxicity using Schrödinger revealed results in line with the in vitro results, thus confirming the bioactivities. The molecule also satisfies the key features of drug likeliness according to pharmacokinetic studies. [ABSTRACT FROM AUTHOR]