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The article focuses on a study which aims at analyzing the validation and calibration of the E-Region Wind Interferometer (ERWIN II). It informs that the ERWIN II combines imaging capabilities of a charge-couple device (CCD) detector and is associated with Michelson interferometry and describes the methodology of the study in which a data analysis algorithm is used to apply the imaging capabilities of the CCD detector.

Summary: Patient engagement in clinical guidelines development is essential. The results of a self-administered online survey identified themes important to people living with osteoporosis and will inform the development of Osteoporosis Canada clinical guidelines recommendations. Introduction: Patient engagement is essential in the development of high-quality and relevant guidelines for osteoporosis management. Osteoporosis Canada (OC) is updating its national clinical practice guidelines in collaboration with people living with osteoporosis in the process. Methods: Using electronic mail, we contacted 6937 members of the Canadian Osteoporosis Patient Network (COPN) to provide input on the selection of relevant content, outcomes, and research questions via a self-administered online survey. Close-ended questions were analyzed using descriptive statistics, and conventional content analysis was conducted for open-ended questions. Results: A total of 1108 individuals completed the survey (97% women, 86% stated they lived with osteoporosis). Most participants considered it critical to have recommendations on physical activity and exercise (74%), fall prevention (69%), nutrition (68%), and initial bone mineral density testing (67%). In addition to preventing fractures, over 75% of respondents stated that consideration of preserving quality of life and ability to perform daily activities, preventing admission to long-term care and fracture-related death, and avoiding serious harms from medications were essential outcomes to consider in evaluating the evidence. In terms of selection of research questions, seven themes emerged from the content analysis including pharmacotherapy, screening and monitoring, diet and supplements, education, exercise, alternative therapies, and pain management. Conclusions: Patients emphasized that autonomy, mobility, and quality of life are highly valued outcomes and must be integral to practice guideline development. As expected, guidance on pharmacotherapy, screening and monitoring, and fracture prevention were priorities identified to be included in osteoporosis management guidelines. [ABSTRACT FROM AUTHOR]

This study compared sclerostin's response to impact versus no-impact high-intensity interval exercise in young men and examined the association between exercise-induced changes in sclerostin and markers of bone turnover and oxidative stress. Twenty healthy men (22.3 ± 2.3 years) performed two high-intensity interval exercise trials (crossover design); running on treadmill and cycling on cycle ergometer. Trials consisted of eight 1 min running or cycling intervals at ≥ 90% of maximal heart rate, separated by 1 min passive recovery intervals. Blood samples were collected at rest (pre-exercise), and 5 min, 1 h, 24 h, and 48 h following each trial. Serum levels of sclerostin, cross-linked telopeptide of type I collagen (CTXI), procollagen type I amino-terminal propeptide (PINP), thiobarbituric acid reactive substances (TBARS), and protein carbonyls (PC) were measured. There was no significant time or exercise mode effect for PINP and PC. A significant time effect was found for sclerostin, CTXI, and TBARS with no significant exercise mode effect and no significant time-by-mode interaction. Sclerostin increased from pre- to 5 min post-exercise (47%, p < 0.05) and returned to baseline within 1 h following the exercise. CTXI increased from pre- to 5 min post-exercise (28%, p < 0.05), then gradually returned to baseline by 48 h. TBARS did not increase significantly from pre- to 5 min post-exercise but significantly decreased from 5 min to 48 h post-exercise. There were no significant correlations between exercise-induced changes in sclerostin and any other marker. In young men, sclerostin's response to high-intensity interval exercise is independent of impact and is not related to changes in bone turnover and oxidative stress markers. [ABSTRACT FROM AUTHOR]

This study examined potential exercise-induced changes in sclerostin and in bone turnover markers in young women following two modes of high intensity interval exercise that involve impact (running) or no-impact (cycling). Healthy, recreationally active, females (n=20; 22.5±2.7 years) performed two exercise trials in random order: high intensity interval running (HIIR) on a treadmill and high intensity interval cycling (HIIC) on a cycle ergometer. Trials consisted of eight 1 min running or cycling intervals at ≥90% of maximal heart rate, separated by 1 min passive recovery intervals. Blood samples were collected at rest (pre-exercise) and 5 min, 1h, 24h, and 48h following each exercise trial. Serum was analyzed for sclerostin, cross linked telopeptide of type I collagen (CTXI), and procollagen type I amino-terminal propeptide (PINP). A significant time effect was found for sclerostin, which increased from pre-exercise to 5 min after exercise in both trials (100.2 to 131.6 pg/ml in HIIR; 102.3 to 135.8 pg/ml in HIIC, p<0.001) and returned to baseline levels by 1h, with no difference between exercise modes and no exercise mode-by-time interaction. CTXI did not significantly change following either trial. PINP showed an overall time effect following HIIR, but none of the post hoc pairwise comparisons were statistically significant. In young women, a single bout of high intensity exercise induces an increase in serum sclerostin, irrespective of exercise mode (impact versus no-impact), but this response is not accompanied by a response in either bone formation or resorption markers. [ABSTRACT FROM AUTHOR]

New Findings What is the central question of this study? The aim was to determine whether mitochondrial protein content of perilipin 3 (PLIN3) and perilipin 5 (PLIN5) is increased following endurance training and whether mitochondrial PLIN5 protein is increased to a greater extent in endurance-trained rats when compared with sedentary rats following acute contraction., What is the main finding and its importance? Mitochondrial PLIN3 but not PLIN5 protein was increased in endurance-trained compared with sedentary rats, suggesting a mitochondrial role for PLIN3 due to chronic exercise. Contrary to our hypothesis, acute mitochondrial PLIN5 protein was similar in both sedentary and endurance-trained rats., Endurance training results in an increased association between skeletal muscle lipid droplets and mitochondria. This association is likely to be important for the expected increase in intramuscular fatty acid oxidation that occurs with endurance training. The perilipin family of lipid droplet proteins, PLIN(2-5), are thought to play a role in skeletal muscle lipolysis. Recently, results from our laboratory demonstrated that skeletal muscle mitochondria contain PLIN3 and PLIN5 protein. Furthermore, 30 min of stimulated contraction induces an increased mitochondrial PLIN5 content. To determine whether mitochondrial content of PLIN3 and PLIN5 is altered with endurance training, Sprague-Dawley rats were randomized into sedentary or endurance-trained groups for 8 weeks of treadmill running followed by an acute (30 min) sciatic nerve stimulation to induce lipolysis. Mitochondrial PLIN3 protein was ∼1.5-fold higher in red gastrocnemius of endurance-trained rats compared with sedentary animals, with no change in mitochondrial PLIN5 protein. In addition, there was an increase in plantaris intramuscular lipid storage. Acute electrically stimulated contraction in red gastrocnemius from sedentary and endurance-trained rats resulted in a similar increase of mitochondrial PLIN5 between these two groups, with no net change in PLIN3 in either group. Plantaris intramuscular lipid content decreased to a similar extent in sedentary and endurance-trained rats. These results suggest that while total mitochondrial PLIN5 content is not altered by endurance training, PLIN5 does have an acute role in the mitochondrial fraction during muscle contraction. Conversely, mitochondrial PLIN3 does not change acutely with muscle contraction, but PLIN3 content was increased following endurance training, indicating a role in chronic adaptations of skeletal muscle. [ABSTRACT FROM AUTHOR]

Operation of a Meteor Wind Radar (MWR) at Eureka, Ellesmere Island (80° N, 86° W) began in February 2006; this is the location of the Polar Environmental and Atmospheric Research Laboratory (PEARL), operated by the "Canadian Network for the Detection of Atmospheric Change" (CANDAC). The first 36 months of wind data (82- 97 km) are here combined with contemporaneous winds from the Meteor Wind Radar at Adventdalen, Svalbard (78° N, 16° E), to provide the first evidence for substantial interannual variability (IAV) of longitudinally spaced observations of mean/background winds and waves at such High Arctic latitudes. The influences of "Sudden Stratospheric Warmings" (SSW) are also apparent. Monthly meridional (north-south, NS) 3-year means for each location/radar demonstrate that winds (82-97 km) differ significantly between Canada and Norway, with winterequinox values generally northward over Eureka and southward over Svalbard. Using January 2008 as case study, these oppositely directed meridional winds are related to mean positions of the Arctic mesospheric vortex. The vortex is from the Canadian Middle Atmosphere Model, with its Data Assimilation System (CMAM-DAS). The characteristics of "Sudden stratospheric Warmings" SSW in each of the three winters are noted, as well as their uniquely distinctive shortterm mesospheric wind disturbances. Comparisons of the mean winds over 36 months at 78 and 80_ N, with those within CMAM-DAS, are featured. E.g. for 2007, while both monthly mean EW and NS winds from CMAM/radar are quite similar over Eureka (82-88 km), the modeled autumn-winter NS winds over Svalbard (73-88 km) differ significantly from observations. The latter are southward, and the modeled winds over Svalbard are predominately northward. The mean positions of the winter polar vortex are related to these differences. [ABSTRACT FROM AUTHOR]

Operation of a Meteor Radar (MWR) at Eureka, Ellesmere Island (80° N, 86° W) began in February 2006: this is the location of the Polar Environmental and Atmospheric Research Laboratory (PEARL), operated by the "Canadian Network for the Detection of Atmospheric Change" (CANDAC). The first 36 months of tidal wind data (82-97 km) are here combined with contemporaneous tides from the Meteor Radar (MWR) at Adventdalen, Svalbard (78° N, 16° E), to provide the first significant evidence for interannual variability (IAV) of the High Arctic's diurnal and semidiurnal migrating (MT) and non-migrating tides (NMT). The three-year monthly means for both diurnal (DT) and semi-diurnal (SDT) winds demonstrate significantly different amplitudes and phases at Eureka and Svalbard. Typically the summer-maximizing DT is much larger (∼24ms-1 at 97 km) at Eureka, while the Svalbard tide (5-24ms-1 at 97 km)) is almost linear (north-south) rather than circular. Interannual variations are smallest in the summer and autumn months. The High Arctic SDT has maxima centred on August/September, followed in size by the winter features; and is much larger at Svalbard (24ms-1 at 97 km, versus 14-18ms-1 in central Canada). Depending on the location, the IAV are largest in spring/winter (Eureka) and summer/autumn (Svalbard). Fitting of wave-numbers for the migrating and nonmigrating tides (MT, NMT) determines dominant tides for each month and height. Existence of NMT is consis- tent with nonlinear interactions between migrating tides and (quasi) stationary planetary wave (SPW) S =1 (SPW1). For the diurnal oscillation, NMT s = 0 for the east-west (EW) wind component dominates (largest tide) in the late autumn and winter (November-February); and s =+2 is frequently seen in the north-south (NS) wind component for the same months. The semi-diurnal oscillation's NMT s =+1 dominates from March to June/July. There are patches of s =+3 and +1, in the late fall-winter. These wave numbers are also consistent with SPW1-MT interactions. Comparisons for 2007 of the observed DT and SDT at 78- 80° N, with those within the Canadian Middle Atmosphere Model Data Assimilation System CMAM-DAS, are a major feature of this paper. The diurnal tides for the two locations have important similarities as observed and modeled, with seasonal maxima in the mesosphere from April to October, and similar phases with long/evanescent wavelengths. However, differences are also significant: observed Eureka amplitudes are generally larger than the model; and at Svalbard the modeled tide is classically circular, rather than anomalous. For the semi-diurnal tide, the amplitudes and phases differ markedly between Eureka and Svalbard for both MWR-radar data and CMAM-DAS data. The seasonal variations from observed and modeled archives also differ at each location. Tidal NMT-amplitudes and wave-numbers for the model differ substantially from observations. [ABSTRACT FROM AUTHOR]