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Cardiac markers in human serum with concentrations less than 0.1 ng/mL were analyzed by use of a guidedmode resonance (GMR) biosensor. Cardiac troponin I (cTnI), creatine kinase MB (CK-MB), and myoglobin (MYO) were monitored in the serum of both patients and healthy controls. Dose-response curves ranging from 0.05 to 10 ng/mLforclnl, from 0.1 to 10 ng/mL for CK-MB, and from 0.03 to 1.7 pg/mL for MYO were obtained. The limits of detection (LOD) for cTnI, CK-MB, and MYO were less than 0.05, 0.1, and 35 μg/mL, respectively. Analysis time was 30 mm, which is short enough to meet clinical requirements. Antibody immobilization and the hydrophilic properties of the guided-mode resonance filter (GMRF) surface were investigated by X-ray photoelectron spectroscopy (XPS) and by monitoring the peak wavelength shift and water contact angle (CA). Both assays used to evaluate the surface density of the immobilized antibodies, a sandwich enzyme-linked immunosothent assay (EUSA) and a sandwich immunogold assay, showed that the antibodies were successfully immobilized and sufficiently aligned to detect the low concentration of biomarkers. Our results show that the GMR biosensor will be veiy useful in developing low-cost portable biosensors that can screen for cardiac diseases. [ABSTRACT FROM AUTHOR]

An antibody immobilization was investigated using a self-assembled monolayer (SAM) over the highly refractive coatings with a SiO2, TiO2, or Si3N4substrate. The immobilization was characterized by analyzing the hydrophilic properties of hydroxyl (OH) groups on surface coatings with contact angle (CA) measurements to enhance protein immobilization. The hydroxyl (OH) group was formed in greater amounts as the oxygen plasma exposure time was increased, which resulted in a large enhancement in antibody immobilization. It indicated that hydroxyl (OH) group formation is critical for developing a label-free optical transducer with a high sensitivity. [ABSTRACT FROM AUTHOR]

Gold nanoparticles exhibited strong surface plasmon absorption and couplings between neighboring particles within bioactivated self-assembly modified their optical properties. Colorimetric analysis on the optical modification of surface plasmon resoanance (SPR) shift and flocculation parameter functionalized bioinspired gold assembly for biophotonic application. The physical origin of bioinspired gold aggregation-induced shifting, decreasing, or broadening of the plasmon absorption spectra could be explained in terms of dynamic depolarization, collisional damping, and shadowing effects. [ABSTRACT FROM AUTHOR]

The article discusses the result of nodakenin, a significant natural resource and anti-allergic and anti-inflammatory medicinal material on atopic dermatitis-like skin lesions. It states the investigation of inhibitory effect of nodakenin on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD)-like skin lesions in imprinting control region (ICR) mice. An overview on the study involving the animal care conducted in the Yonsei University at Wonju, South Korea is also presented.

Anionic sulfate (SO4−)-functionalized polystyrene (PS) nanoparticles were prepared by the thermal decomposion of potassium persulfate (KPS) in the presence of sodium tetraborate via emulsion polymerization. The presence of a SO4−group at a solid/liquid interface of a particle surface was confirmed by a zeta potential value of −40.6 mV as well as the shifting of S 2p spectra toward a lower-binding-energy region around 162.7 eV (2p3/2) and 164.4 eV (2p1/2) in X-ray photoelectron spectroscopy (XPS) analysis. The electrostatic attraction between positively charged antibodies of human immunoglobulin G (hIgG) and cardiac troponin I (cTnI) and negatively charged particle surfaces was accomplished. The atomic force microscopy (AFM) measurement and bicinchoninic acid (BCA) assay results show binding structure between hIgG and antibodies of hIgG (anti-hIgG) with a gradual increase in particle diameter to 152.6 nm (bare), 170.2 nm (hIgG), and 178.9 nm (hIgG/anti-hIgG). Surface coverage densities of 331.4 ng/cm2(hIgG) and 320.3 ng/cm2(cTnI) and the binding capacity of hIgG to HyLite-750-labeled Fab-specific anti-hIgG (∼81.2%) indicate that the majority of hIgG was immobilized with a Y-shaped orientation. The sandwich immunoassay results provide the evidence that the immunological activity of cTnI on the PS nanoparticle surface was retained because the binding activity of the cTnI-PS nanoparticle/cTnI (antigen)/detection cTnI-antibody reaction showed a 5-fold higher activity than that of the cTnI-PS nanoparticle/human serum albumin (HSA)/detection cTnI antibody used as a negative control. [ABSTRACT FROM AUTHOR]