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The interaction between proteins and solid surfaces can influence their conformation and therefore also their activity and affinity. These interactions are highly specific for the respective combination of proteins and solids. Consequently, it is desirable to investigate the conformation of proteins on technical surfaces, ideally at single molecule level, and to correlate the results with their activity. This is in particular true for biosensors where the conformation-dependent target affinity of an immobilized receptor determines the sensitivity of the sensor. Here, we investigate for the first time the immobilization and orientation of antibodies (Abs) photoactivated by a photonic immobilization technique (PIT), which has previously demonstrated to enhance binding capabilities of antibody receptors. The photoactivated immunoglobulins are immobilized on ultrasmooth template stripped gold films and investigated by atomic force microscopy (AFM) at the level of individual molecules. The observed protein orientations are compared with results of nonactivated antibodies adsorbed on similar gold films and mica reference samples. We find that the behavior of Abs is similar for mica and gold when the protein are not treated (physisorption), whereas smaller contact area and larger heights are measured when Abs are treated (PIT). This is explained by assuming that the activated antibodies tend to be more upright compared with nonirradiated ones, thereby providing a better exposure of the binding sites. This finding matches the observed enhancement of Abs binding efficiency when PIT is used to functionalize gold surface of QCM-based biosensors. [ABSTRACT FROM AUTHOR]

Hydrophobins (HFBs) are peculiar proteins which self-assemble at hydrophilic-hydrophobic interfaces into amphipathic membranes, and some of them (class I HFB) are able to form much more stable amyloid-like layers. This feature makes them suitable for many purposes, particularly when stable surface functionalization is required, also in view of their versatility in binding different kinds of molecules. For instance, it has been shown that Vmh2 from Pleurotus ostreatus (a class I HFB) is able to bind molecules like glucose, thus offering the perspective of using Vmh2 as a surface functionalization tool in bio-hybrid devices. In this paper a quantitative analysis of glucose interaction with the Vmh2 layer is reported; in particular, it is shown that Vmh2 layer swells by almost doubling its thickness as a result of glucose diffusion and each Vmh2 monomer is able to bind approximately 30 glucose molecules. These results have been achieved by self-assembling multi-layers of Vmh2 on a gold substrate and, subsequently, measuring both the mass of the bound glucose and the thickness of the resulting layer through two different and complementary techniques: quartz crystal-microbalance and ellipsometry. The data provided by the two techniques are in a satisfactory agreement and offer a plausible description of the mechanisms underlying the interaction of glucose with Vmh2 layer. This facile and versatile coating is of interest for biomedical applications of gold surfaces and particles. [ABSTRACT FROM AUTHOR]

We report an application of femtosecond laser ablation to improve the sensitivity of biosensors based on a quartz crystal microbalance device. The nanoparticles produced by irradiating a gold target with 527-nm, 300-fs laser pulses, in high vacuum, are directly deposited on the quartz crystal microbalance electrode. Different gold electrodes are fabricated by varying the deposition time, thus addressing how the nanoparticles surface coverage influences the sensor response. The modified biosensor is tested by weighting immobilized IgG antibody from goat and its analyte (IgG from mouse), and the results are compared with a standard electrode. A substantial increase of biosensor sensitivity is achieved, thus demonstrating that femtosecond laser ablation and deposition is a viable physical method to improve the biosensor sensitivity by means of nanostructured electrodes. [ABSTRACT FROM AUTHOR]

The public has become increasingly interested in the welfare of food animals, but the food animal industries possess few mechanisms for public engagement. Here we present results from a web-based forum designed to allow stakeholders to share views on controversial issues in dairying. In response to the question "Should dairy calves be separated from the cow within the first few hours after birth?" participants were able to indicate "yes," "no," or "neutral" and either write a reason in support of their view or select reasons provided by other participants. Four independent groups of participants were recruited (a total of 163 people); 31% said they had no involvement in the dairy industry; the remaining 69% (with some involvement in the industry) were students or teachers (33%), animal advocates (13%), producers (11%), veterinarians (9%) and other dairy industry professionals (3%). Overall, little consensus existed among participants across groups; 44% chose "yes," 48% "no," and 9% "neutral." Responses varied with demographics, with opposition to early separation higher among females, animal advocates, and those with no involvement with the dairy industry. A fifth group was recruited at a dairy industry conference (an additional 28 participants); 46% chose "yes," 32% "no," and 21% "neutral." Across all 5 groups, opponents and supporters often referenced similar issues in the reasons they provided. Opponents of early separation contended that it is emotionally stressful for the calf and cow, it compromises calf and cow health, it is unnatural, and the industry can and should accommodate cow-calf pairs. In contrast, supporters of early separation reasoned that emotional distress is minimized by separating before bonds develop, that it promotes calf and cow health, and that the industry is limited in its ability to accommodate cow-calf pairs. These results illustrate the potential of web-based forums to identify areas of agreement and conflict among stakeholders, providing a basis for the development of practices that address shared concerns. Key words: animal welfare, producer attitude, maternal filial bond, public engagement [ABSTRACT FROM AUTHOR]

Deterioration of footpad and hock condition and increased stress are important welfare concerns in high-density broiler production. It has been argued that environmental enrichment may help address these issues. We hypothesized that increasing environmental complexity by providing access to barrier perches across a range of densities should improve footpad and hock health, reduce leg fluctuating asymmetry (FA), and mitigate fear in broilers without compromising bird performance. In this study, 2,088 one-day-old broiler chicks were randomly assigned to one of the following barrier and density treatment combinations over 4 rep- lications: simple barrier, complex barrier, or no barrier (control) and low (8 birds/m2), moderate (13 birds/ m2), or high (18 birds/m2) density. In wk 7, data were collected on average tibia width and length, FA of tibia width and tibia length, footpad and hock lesions, tonic immobility, feed conversion, final BW, and mortalities. Broilers at higher densities had more severe footpacl (P < 0.0001) and hock lesions (P < 0.0001). Their tibias were also longer (P < 0.0001) and less symmetric in length (P < 0.05). The tibias of birds in complex bar- rier pens were more symmetric in length compared with controls (P < 0.05). Barrier treatment had no effect on hock lesions, although there was a trend for simple bar- riers to reduce the severity of footpad lesions compared with the control treatment (P 0.089). Birds kept in simple barrier pens were more susceptible to tonic im- mobility induction (P < 0.05). Final BW, feed conver- sion, and mortalities were neither affected by stock- ing density nor by barrier inclusion. In conclusion, this study shows a negative effect of high density on broiler footpad health and FA. Although barrier perches did not appear to reduce fearfulness, the improvement in footpad health suggests that simple barriers may pro- vide key welfare benefits to broiler chickens. [ABSTRACT FROM AUTHOR]

ABSTRACT Apatite fission track ages of 20 samples collected from turbidite successions deposited in foreland basins adjacent to the Northern Apennines range between ∼3 and ∼10 Ma. The youngest fission track ages are concentrated in a NW–SE elongated belt, which approximately runs through the centre of the study area, while gradually increasing ages are distributed towards the south-western and north-eastern borders. Integration of apatite fission track data and published vitrinite reflectance values indicate this region of the Apennines experienced continuous but variable exhumation starting from ∼14 Ma. The extent of exhumation and uplift range between 5 and 6 km at the south-western and north-eastern borders of the study area, and ∼7 km in the central part. Exhumation was driven mainly by erosion, with minor faulting in response to structural readjustment related to differential exhumation. Regional exhumation and erosion are interpreted as the result of isostatic rebound following crustal thickening in the lower part of the orogen. [ABSTRACT FROM AUTHOR]

Aim: To analyze the outcomes of component separation techniques (CST) to treat incisional hernias (IH) in a large multicenter cohort of patients. Methods: All IH repair using CST, registered in EVEREG from July 2012 to December 2019, were included. Data on the pre-operative patient characteristics and comorbidities, IH characteristics, surgical technique, complications, and recurrence were collected. Outcomes between anterior (ACS) and posterior component separation (PCS) techniques were compared. Risk factors for complications and recurrences were analyzed. Results: During the study period, 1536 patients underwent CST (45.5% females) with a median age of 64.0 years and median body mass index (BMI) of 29.7 kg/m2. ACS was the most common technique (77.7%). Overall complications were frequent in both ACS and PCS techniques (36.5%), with a higher frequency of wound infection (10.6% vs. 7.0%; P = 0.05) and skin necrosis (4.4% vs. 0.1%; P < 0.0001) with the ACS technique. Main factors leading to major complications were mesh explant (OR 1.72; P = 0.001), previous repair (OR 0.75; P = 0.038), morbid obesity (OR 0.67; P = 0.015), ASA grade (OR 0.62; P < 0.0001), COPD (OR 0.52; P < 0.0001), and longitudinal diameter larger than 10 cm (OR 0.58; P = 0.001). After a minimum follow-up of 6 months (median 15 months; N = 590), 59 (10.0%) recurrences were diagnosed. Operations performed in a non-specialized unit were significantly associated with recurrences (HR 4.903, CI 1.64–14.65; P = 0.004). Conclusion: CST is a complex procedure with a high rate of complications. Both ACS and PCS techniques have similar complication and recurrence rates. Operations performed in a specialized unit have better outcomes. [ABSTRACT FROM AUTHOR]

Dear Editor, We thank Kumar et al. for their comments and we would like to answer all their observations to clarify the study data results and the design of our study [[1]]. Previous mesh position and relation with component separation technique (CST) are not known in our data since we registered the patients' clinical data: presence of previous hernia repair and presence of previous mesh. However, we strongly believe that our results help to understand outcomes of components separation techniques for hernia repair in our country. [Extracted from the article]

By relying on the photonic immobilization technique of antibodies onto surfaces, we realized portable biosensors for light molecules based on the use of quartz crystal microbalances, given the linear dependence of the method on the laser pulse intensity. Here, we compare the quality of the anchoring method when using nanosecond (260 nm, 25 mJ/pulse, 5 ns, 10 Hz rep. rate) and femtosecond (258 nm, 25 μJ/pulse, 150 fs, 10 kHz rep. rate) laser source, delivering the same energy to the sample with the same average power. As a reference, we also tethered untreated antibodies by means of the passive adsorption. The results are striking: When the antibodies are irradiated with the femtosecond pulses, the deposition on the gold plate is much more ordered than in the other two cases. The effects of UV pulses irradiation onto the antibodies are also analyzed by measuring absorption and fluorescence and suggest the occurrence of remarkable degradation when nanosecond pulses are used likely induced by a larger thermal coupling. In view of the high average power required to activate the antibodies for the achievement of the photonic immobilization technique, we conclude that femtosecond rather than nanosecond laser pulses have to be used. [ABSTRACT FROM AUTHOR]

Purpose: The small bites (SB) technique for closure of elective midline laparotomies (EMLs) and a prophylactic mesh (PM) in high-risk patients are suggested by the guidelines to prevent incisional hernias (IHs) and fascial dehiscence (FD). Our aim was to implement a protocol combining both the techniques and to analyze its outcomes. Methods: Prospective data of all EMLs were collected for 2 years. Results were analyzed at 1 month and during follow-up. The incidence of HI and FD was compared by groups (M = Mesh vs. S = suture) and by subgroups depending on using SB. Results: A lower number of FD appeared in the M group (OR 0.0692; 95% CI 0.008–0.56; P = 0.01) in 197 operations. After a mean follow-up of 29.23 months (N = 163; min. 6 months), with a lower frequency of IH in M group (OR 0.769; 95% CI 0.65–0.91; P < 0.0001). (33) The observed differences persisted after a propensity matching score: FD (OR 0.355; 95% CI 0.255–0.494; P < 0.0001) and IH (OR 0.394; 95% CI 0.24–0.61; P < 0.0001). On comparing suturing techniques by subgroups, both mesh subgroups had better outcomes. PM was the main factor related to the reduction of IH (HR 11.794; 95% CI 4.29–32.39; P < 0.0001). Conclusion: Following the protocol using PM and SB showed a lower rate of FD and HI. A PM is safe and effective for the prevention of both HI and FD after MLE, regardless of the closure technique used. [ABSTRACT FROM AUTHOR]

Abstract: Glucose oxidase (GOD) immobilized into sol–gel matrices was studied by using Micro-Attenuated Total Reflection Fourier Transform Infrared (micro-ATR FT-IR) spectroscopy in order to characterize enzyme distribution and secondary structure in systems with valuable potentialities in amperometric and optical biosensing. Spectra were acquired in the 4000–600cm−1 frequency region and the analysis of specific fingerprints in the FT-IR spectra evidenced that the enzyme was actually immobilized in the matrix. The enzyme spatial distribution was obtained by examining the amide I and amide II band region of spectra from defined sample positions. The deconvolution of the amide I band in terms of lorentzian functions provided information on the secondary structure of the immobilized GOD. By this approach a macroscopic preservation of GOD activity upon immobilization was evidenced along with the existence of some matrix sites with locally inactivated GOD. To our knowledge this is the first example of point-by-point characterization of conformational changes of immobilized enzyme by means of micro-ATR infrared spectroscopy, thus confirming that this technique can be usefully employed for a non- or minimally-invasive detailed micro-characterization of catalytic supports in order to improve their functionality. [Copyright &y& Elsevier]

Apatite fission-track analyses along a W–E-orientated transect across northern Corsica indicate an important episode of crustal exhumation in late early Miocene time. Samples taken from the Alpine orogenic wedge, from the adjacent foreland basin and from the crystalline basement complex flooring the basin are completely reset. This implies that a ≥ 2.0–2.3-km-thick crustal section made of thrust sheets and/or autochthonous foreland deposits has been removed by erosion since early Miocene time. A geometric projection of this lost cover towards the west indicates that all of northern Corsica was covered either by Alpine nappes or middle Eocene foreland deposits. Fission-track ages are the same across the main boundary fault system separating the Alpine orogenic wedge and the foreland, indicating the absence of significant differential vertical displacement between upper and lower plates during Neogene unroofing. [ABSTRACT FROM AUTHOR]

Coordination cages obtained upon complexation of pyridyl functionalized porphyrins by Ag+ disassemble when overtaking a 1 : 2 stoichiometric amount of silver salt. An excess of Ag+ then leads to unusual chemical processes, here analyzed in detail, which permanently transform the monomeric porphyrins. The observed processes, discussed with reference to model compounds devoid of polyether substituted pyridyl residues, evidence a peculiar reactivity for meso 2,6-dimethylphenyl substituted porphyrins. [ABSTRACT FROM AUTHOR]

Solid [CuI(piperazine)0.5]∞, characterized by a structure with an infinite double chain of CuI, presents an unexpected dual luminescence. The short copper–copper distances allow the existence of both cluster-centered and 1-D delocalized electronic transitions, as emerged from theoretical calculations. Beyond the more common cluster-centered emission a higher energy band, which differs in lifetime and in temperature dependence, is observed. [ABSTRACT FROM AUTHOR]

Highlights: [•] We model initial wave generation on Titan’s hydrocarbon lakes and seas. [•] The effects of gravity, density, viscosity, and surface tension are quantified. [•] The threshold wind (at 10m) for wave generation in liquid ethane on Titan is 0.6m/s. [•] A two-scale model is constructed to predict radar backscatter of wind-waves on Titan. [•] Winds may exceed the min. threshold for exciting waves during Titan’s Spring/Summer. [ABSTRACT FROM AUTHOR]

The objective of this study was to infer soil moisture variability from a combination of passive microwave and infrared satellite observations. The proposed approach is mainly based on the concept of apparent thermal inertia (ATI) and makes use of the daily gradient in brightness temperature from MODIS AQUA to infer soil moisture at moderate spatial resolution. Soil moisture retrievals from optical polar orbiting satellites are affected by discontinuities due to the presence of clouds and spurious fluctuations because of low temporal sampling, which is not sufficient for a reliable daily cycle sampling. To mitigate these limitations, we propose using soil moisture temporal trend derived from passive microwave based product, namely the NASA AMSR-E soil moisture product, to filter estimates from MODIS observations. Passive microwave-based soil moisture products exhibit less fluctuation because of their coarse resolution and lower sensitivity to atmosphere. They can therefore be considered as natural "low pass filters" thus reducing the effect of noise in the infrared based estimates. A sensitivity test was conducted to identify to determine the contribution of various factors to the inferred soil moisture from ATI and the error that they may introduce in the estimates. The ATI-based approach was then applied to qualitatively describe the spatial distribution of soil moisture. The algorithm was validated over two different test areas in Italy and France where reference measurements are available. For the test site in Italy, the obtained ATI values were clustered around four different values corresponding to different levels of wetness. The determined four classes of soil moisture (low, medium, medium-high, and high) were compared to available in situ observations. An agreement with in situ observations of 81% was obtained. In densely vegetated areas, only three classes of soil moisture were instead distinguishable. The obtained agreement between observed and inferred soil moisture values was 88%. Also, in the second study area in France, where vegetation is more dominant, only three classes of soil moisture were determined with a lower agreement of 73%. In addition, the ATI trends are in agreement with thermal inertia values determined from physics-based formulation. This study showed that a combination of infrared and passive microwave observation may lead to a better mapping of soil moisture at the regional scale. [ABSTRACT FROM AUTHOR]

Abstract: The potential of Raman spectroscopy with excitation in the visible as a tool for quantitative determination of single components in food industry products was investigated by focusing the attention on glucose content in commercial sport drinks. At this aim, micro-Raman spectra in the 600–1600cm−1 wavenumber shift region of four sport drinks were recorded, showing well defined and separated vibrational fingerprints of the various contained sugars (glucose, fructose and sucrose). By profiting of the spectral separation of some peculiar peaks, glucose content was quantified by using a multivariate statistical analysis based on the interval Partial Least Square (iPLS) approach. The iPLS model needed for data analysis procedure was built by using glucose aqueous solutions at known sugar concentrations as calibration data. This model was then applied to sport drink spectra and gave predicted glucose concentrations in good agreement with the values obtained by using a biochemical assay. These results represent a significant step towards the development of a fast and simple method for the on-line glucose quantification in products of food and beverage industry. [Copyright &y& Elsevier]

Penetrating neck trauma and retained foreign body cause severe injuries. To manage these lesions, the priority is to assess the airway, circulation and neurological status; angiography, Doppler ultrasonography and CT should complement these evaluations. We report the first case of removal of foreign body prevertebral space made at the General hospital Dr. Manuel Gea González through a transoral endoscopic approach, which is valid and less morbid than the external cervical approach. [ABSTRACT FROM AUTHOR]

Abstract: We use Global Positioning System (GPS) velocities and dislocation modeling to investigate the rate and nature of interseismic strain accumulation in the area affected by the 1908 Mw 7.1 Messina earthquake (southern Italy) within the framework of the complex central Mediterranean microplate kinematics. Our data confirm a change in the velocity trends between Sicily and Calabria, moving from NNW-ward to NE-ward with respect to Eurasia, and detail a fan-like pattern across the Messina Straits where maximum extensional strain rates are ~65 nanostrains/yr. Extension normal to the coast of northern Sicily is consistent with the presence of SW–NE trending normal faults. Half-space dislocation models of the GPS velocities are used to infer the slip-rates and geometric fault parameters of the fault zone that ruptured in the Messina earthquake. The inversion, and the bootstrap analysis of model uncertainties, finds optimal values of 3.5 +2.0 −1.3 and 1.6 +0.3 −0.2 mm/yr for the dip–slip and strike–slip components, respectively, along a 30 +1.1 −0.7° SE-ward dipping normal fault, locked above 7.6 +4.6 −2.9 km depth. By developing a regional elastic block model that accounts for both crustal block rotations and strain loading at block-bounding faults, and adopting two different competing models for the Ionian–Calabria convergence rates, we show that the measured velocity gradient across the Messina Straits may be significantly affected by the elastic strain contribution from other nearby faults. In particular, when considering the contribution of the possibly locked Calabrian subduction interface onto the observed velocity gradients in NE-Sicily and western Calabria, we find that this longer wavelength signal can be presently super-imposed on the observed velocity gradients in NE-Sicily and Calabria. The inferred slip-rate on the Messina Fault is significantly impacted by elastic strain from the subduction thrust. By varying the locking of the subduction thrust fault, in fact, the Messina Fault slip-rate varies from 0 to 9mm/yr. [Copyright &y& Elsevier]

The European Alps are a mountain belt that is characterized by a series of discrete orogenic events, which have long been recognized. Despite the inherent episodic nature of orogenic evolution, the Alps have been continuously exhumed, mainly by erosion, but also by normal faulting. Since continental collision started in the late Eocene/Early Oligocene evidence for ongoing erosional exhumation has been preserved in synorogenic sediments that accumulated in basins adjacent to the pro- and retro-side of this double-vergent mountain belt. This long-term erosion record can be used to determine exhumation rates. Lag-times calculated from fission-track (FT) ages of detrital zircon from synorogenic sediments are fairly constant for the European Alps since the Oligocene–Late Miocene. Although the fast exhuming areas were unroofed at rates of 0.4–0.7 km Myr−1, the overall average exhumation rate is between 0.2 and 0.3 km Myr−1 on a regional scale. The detrital and bedrock zircon FT data of the Alps do not detect the increase in erosion rates since the Pliocene over the past ∼5 Myr, as shown elsewhere. This increase cannot be detected yet with the detrital zircon FT method because not enough rock has been removed to widely expose zircons with Pliocene or younger cooling ages in the Alps. Long term (30 Myr) exhumation rates appear to have been approximately constant when averaged over a sliding time window of about 8 Myr, or depth window of 5 to 10 km (ZFT closure depths); shorter-term fluctuations are not identified using this method. [ABSTRACT FROM AUTHOR]

Numerous studies suggest that silicon (Si) supplementation is beneficial for mineral metabolism and bone health. Mineral balance studies have not been performed in horses to determine how these supplements affect absorption of other minerals. The purpose of these studies was to investigate the effects of two different Si supplements on mineral absorption and retention in horses. Eight geldings were randomly placed in one of two groups: control (CO) or supplemental Si, which was provided by one of two supplements. The first, sodium aluminium silicate (SA), contains a bioavailable form of Si and is high in aluminium (Al). The second supplement contains oligomeric orthosilicic acid (OSA). All horses received textured feed and ad libitum access to hay. Supplemented horses received either 200 g of SA or 28.6 ml of OSA per day. Following a 10-day adaptation period, the horses underwent a 3-day total collection. Blood samples were taken on days 0 and 13. The two balance studies were conducted 4 months apart to reduce carryover effects. Intakes of Al and Si were greater with SA supplementation (p < 0.05). Sodium aluminium silicate increased faecal and urinary Si excretion (p < 0.05). Calcium retention and apparent digestion were increased by SA (p < 0.05). It also maintained plasma Si compared with the CO which tended to have a decrease in plasma Si (p = 0.08). Supplemental OSA increased retention of Ca and B (p < 0.05) and apparent digestion of B (p < 0.01). Orthosilicic acid tended to increase Si retention (p = 0.054), apparent digestion (p < 0.065), and also increased plasma Si. Both supplements were able to alter Ca retention and B metabolism, however, only OSA was able to alter Si retention, digestibility and plasma concentration. Orthosilicic acid, an Si supplement without substantial Al, appears to be a viable option for Si supplementation as it increased Si retention and digestibility. [ABSTRACT FROM AUTHOR]