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Background: Ficus carica is an edible fruit, belonging to the Moraceae family, rarely described as cause of food allergy. We describe the first case of fig allergy that occurred as a cross-reactivity between fig and Derp 1. Case presentation: We present a case of a 10-years-old-girl, with a history of no-seasonal mild intermittent rhinitis, who experienced an immediate reaction after ingestion of a fresh fig. Skin prick tests (SPT) with commercial extracts of food, airborne allergens, latex and panallergens (profilin, PR-10 and lipid transfer protein) were performed. SPT revealed a sensitization only for dermatophagoides farina and dermatophagoides pteronyssinus which was then confirmed with by specific IgE assay (UniCAP, Phadia, Uppsala, Sweden). We also carried out a positive SPT with a commercial fig allergen (Lofarma, Milan, Italy) and prick-by-prick (PBP) both with skin and pulp of green raw and cooked fig. Fig specific serum IgE levels were 1.08 U/ml and specific IgE for rDer p1 was 16.20 U/ml (total serum IgE = 377 U/ml). In contrast specific IgE levels for latex, LTP, profilin, PR-10 and pollen allergens were negative. Conclusion: The ficin, the major fig allergen, belongs to cysteine protease family like Der p 1. The symptoms presented by our patient could be related to a cross reactivity between these two proteins which present a structural homology. [ABSTRACT FROM AUTHOR]

Understanding the mechanisms of shallow magma storage (less than ~10‐km depth) is crucial to defining volcanic plumbing systems and their effect on volcano unrest. Sills are common structures to emplace shallow magma. Many factors may control sill emplacement, but their relative importance has not been evaluated so far. To define a hierarchy among a selection of the proposed factors, we performed analogue models by injecting water at constant flux (magma analogue) within gelatin (crust analogue), investigating the effects of: interface strength and rigidity contrast between layers, density layering, ratio of layer thickness, compressive stresses, magma flow rate, and buoyancy pressure. Our results show that a strong rigidity contrast (i.e., a stiff layer overlying a weak one) and a higher buoyancy pressure are necessary and sufficient conditions for sill emplacement. Low rigidity contrasts require other contributions (weak interface and compressive stress) to develop sills; however, without rigidity contrast sill formation is always inhibited. These experiments suggest that sill emplacement is primarily controlled by rigidity contrasts and magma buoyancy pressure; the second‐order parameters are lateral compression and weak interfaces; the third‐order parameters are the ratio of layer thicknesses, magma flow rate, and density layering. These results are in agreement with field observations in Iceland, Tenerife, Utah, and La Réunion, supporting our proposed hierarchy. Key Points: Understanding what controls a dike to sill rotation is important to improve our knowledge on magma storage in the upper crustWe have defined a hierarchy among selected parameters controlling sill emplacement to understanding shallow magma transferAnalogue models show that high rigidity contrast and magma buoyancy pressure are necessary and sufficient conditions for sill emplacement [ABSTRACT FROM AUTHOR]

Abstract: Volcanic eruptions are usually fed by dikes. Understanding how crustal inhomogeneities and topographic loads control the direction (lateral/vertical) and extent (propagation/arrest) of dikes is crucial to forecast the opening of a vent. Many factors, including buoyancy, crustal layering, and topography, may control the vertical or lateral propagation of a dike. To define a hierarchy between these factors, we have conducted analogue models, injecting water (magma analogue) within gelatin (crust analogue). We investigate the effect of crustal layering (both rigidity and density layering), topography, magma inflow rate, and the density ratio between host rock and magma. Based on the experimental observations and scaling considerations, we suggest that rigidity layering (a stiffer layer overlying a weaker one) and topographic gradient favor predominantly lateral dike propagation; inflow rate, density layering, and density ratio play a subordinate role. Conversely, a softer layer overlying a stiffer one favors vertical propagation. Our results highlight the higher efficiency of a stiff layer in driving lateral dike propagation and/or inhibiting vertical propagation with respect to the Level of Neutral Buoyancy proposed by previous studies. [ABSTRACT FROM AUTHOR]

Junctional adhesion molecules (JAMs) are a family of adhesion molecules localized at the tight junction of polarized cells and on the cell surface of leukocytes. The last 20 years of research in this field has shown that several members of the family play an important role in the regulation of cell polarity, endothelium permeability and leukocytes migration. They mediate these pleiotropic functions through a multitude of homophilic and heterophilic interactions with intrafamily and extrafamily partners. In this article, we review the current status of the JAM family and highlight their functional role in tight junction dynamics and leukocyte transmigration. [ABSTRACT FROM AUTHOR]

Atherosclerosis is a complex chronic inflammatory and metabolic disease that involves the collaboration of several cellular components of the immune system and results in thickening of the arterial wall. Atherosclerosis is also the primary cause of coronary artery and cerebrovascular diseases. A multitude of immune cell subsets, soluble molecules such as chemokines and cytokines, and circulating lipids play pivotal roles in atherosclerosis development. In this review, we highlight the role of the immune system in the course of atherosclerotic disease development and discuss the mechanisms involved. [ABSTRACT FROM AUTHOR]

The low-density lipoprotein receptor-related protein 1 ( LRP1) is an ubiquitously expressed endocytic receptor that, among its several functions, is involved in the catabolism of coagulation factor VIII ( FVIII) and in the regulation of its plasma concentrations. Although LRP1/ CD91 polymorphisms have been associated with increased FVIII levels and a consequent thrombotic risk, no data are available on LRP1/ CD91 expression in patients with inherited FVIII deficiency. With the aim of elucidating this issue, 45 consecutive patients with haemophilia A ( HA) (18 severe, 5 moderate and 22 mild HA) were enrolled in this cross-sectional, single-centre survey. The LRP1/ CD91 mean fluorescence intensity ( MFI) in monocytes from HA patients was significantly higher than that detected in 90 healthy blood donors (105 vs. 67, P < 0.001). This over-expression was independent of hepatitis C virus infection status and varied according to the severity of the haemophilia, being higher in patients with more severe FVIII deficiency. In conclusion, our study documents for the first time that LRP1/ CD91 is over-expressed on monocytes from HA patients, with the intensity of expression varying according to the severity of the FVIII deficiency. Further studies are needed to assess the clinical implications of these findings. [ABSTRACT FROM AUTHOR]

The author examines the role of reactive oxygen species (ROS)-producing enzyme, Nox4, in tumor progression and angiogenesis. The author explains the process of angiogenesis, the process in which tumors become hypoxic and the composition of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complexes and how they affect tumor progression of slow-growing tumors.

Common fragile sites (CFSs) are regions of chromosomal break that may play a role in oncogenesis. The most frequent alteration occurs at FRA3B, within the FHIT gene, at chromosomal region 3p14. We studied a series of breast carcinomas for break of a CFS at 6q26, FRA6E, and its associated gene PARK2, using fluorescence in situ hybridization on tissue microarrays (TMA). We found break of PARK2 in 6% of cases. We studied the PARK2-encoded protein Parkin by using immunohistochemistry on the same TMA. Loss of Parkin was found in 13% of samples but was not correlated with PARK2 break. PARK2 break but not Parkin expression was correlated with prognosis. Alteration of PARK2/FRA6E may cause haplo-insufficiency of one or several telomeric potential tumor suppressor genes (TSG). The AF-6/MLLT4 gene, telomeric of PARK2, encodes the Afadin scaffold protein, which is essential for epithelial integrity. Loss of Afadin was found in 14.5% of cases, and 36% of these cases showed PARK2 break. Loss of Afadin had prognostic impact, suggesting that AF-6 may be a TSG. Loss of Afadin was correlated with loss of FHIT expression, suggesting fragility of FRA6E and FRA3B in a certain proportion of breast tumors.Oncogene (2007) 26, 298–307. doi:10.1038/sj.onc.1209772; published online 3 July 2006 [ABSTRACT FROM AUTHOR]

Background Mesenchymal stem cells (MSC) are multipotent progenitors retaining the capability to undergo multilineage differentiation, mostly towards all the mesodermal cellular lineages. MSC growing under standard conditions are composed of two main subpopulations with a characteristic distribution in the morphologic flow cytometric scatter: RS (recycling stem) cells (small, agranular) and m (mature) MSC (large, moderately granular cells). Methods MSC obtained from BM of healthy donors and expanded in culture were characterized by evaluating both the expression of conventional markers and differentiation potential. We used CFSE, a lipophilic dye that is taken up by cell membranes, to investigate separately the proliferative activity of RS cells and mMSC subsets. Results With flow cytometric analysis, RS cells and mMSC showed nearly the same immunophenotypic pattern, even if a significantly smaller percentage of RS cells expressed some of the classic mesenchymal Ag. The RS cell fraction was confirmed to have a higher proliferative potential and such a feature was particularly evident under certain culture conditions. Discussion CFSE has been shown as a reliable method for studying the proliferative activity of MSC subpopulations identified by flow cytometric analysis. The acquisition parameter strategy is crucial for the accuracy of the analysis [ABSTRACT FROM AUTHOR]

Magmatic necks are commonly found in volcanic areas, and they often exhibit a homogeneous structure with a cylindrical shape and a diameter of up to several hundreds of metres. Their massive and uniform structure poses a space problem for their emplacement in the brittle crust. Here, we use field data and analogue models to investigate how necks may emplace at shallow levels. Field analysis focuses on characterising the geometric, structural and magmatic features of two necks outcropping in the eroded portions of Mt. Etna, Italy. These are homogeneous and massive intrusive bodies, related to a single episode of emplacement at 400-600 m below the paleosurface. We further investigated their possible emplacement mechanism through analogue models, injecting vegetable oil within (a) a flat sand pack and (b) a sand cone. Dikes form with both configurations, erupting to the surface through vents. However, dikes injected within the cone are characterised by a larger thickening at shallow levels, in correspondence with the vent, where a neck-like structure forms. This suggests that the gravitational load imposed by a volcanic edifice provides the most suitable conditions for the development of magmatic neck, as the downslope shear stresses enhance the deformation of the cone slope during shallow dike emplacement promoting shallow dilation and thickening of the dike. Therefore, topography should be a further factor enhancing the development of necks, in addition to those mechanisms previously proposed. Our results are consistent with natural examples of feeder dikes thickening towards the surface and dikes transitioning to necks, supporting the reliability of the proposed conceptual model. [ABSTRACT FROM AUTHOR]

Anisotropy of Magnetic Susceptibility (AMS) is a valid tool to investigate magma flow direction within dikes. However, geometrically inverse magnetic fabric characterized by maximum magnetic susceptibility axis (kmax) perpendicular to the dike wall may complicate the interpretation of flow trajectories. To better understand the nature of this fabric, we present a multiscale study on 19 dikes (383 samples) in the Miocene Alftafjordur volcanic system (Iceland), where 80% of the samples show a geometrically inverse magnetic fabric. We carried out (1) AMS measurements at different magnetic fields and temperatures, along with Anisotropy of Anhysteretic Remanent Magnetization (AARM) analysis; (2) hysteresis loops and FORC diagrams; (3) thin section analysis; (4) structural fieldwork. A variable Ti‐content (0.1 < x < 0.6, Fe3‐xTixO4) titanomagnetite is the main magnetic carrier, and the contribution of the paramagnetic elongated crystals to the magnetic fabric is negligible. Single domain is not the prevailing domain state of the magnetic particles, suggesting that its occurrence cannot be the main cause for the inverse fabric. AMS analysis at different fields and temperatures along with AARM allow us to exclude any mineral phase change of the titanomagnetite across the dike. Nevertheless, kmax is parallel to a diffuse horizontal column‐like fracture pattern perpendicularly oriented with respect to the dike strike. This suggests that the Ti‐magnetite mineral orientation during dike cooling was affected by the fracture network progressively developing columnar basalts. This study demonstrates that the interpretation of AMS data on old and deep volcanic bodies is not straightforward and observations at different scales are required. Key Points: Our analyses about the magnetic fabric of the Alftafjordur dike swarm revealed that most of the samples show an inverse magnetic fabricThe origin of the inverse magnetic fabric is associated with the development of horizontal fractures during the cooling stage of dikesIt is not straightforward to infer the magma flow direction on deep and old exhumed dikes by using standard low‐field AMS measurements only [ABSTRACT FROM AUTHOR]

Introduction. Male breast cancer (MBC) is a rare disease, whose main risk factor is genetic vulnerability. Despite care of men with MBC is modeled on care of women, men's experiences with the disease and concerns related to the status of genetic mutation carrier are unique. So far, little is known concerning the psychological impact in BRCA1/2 testing, especially with regard to specific subset of individuals, such as male subjects and the elderly. Methods. We assessed self-reported anxiety and depression levels in 26 male subjects presenting at Unit of Breast Surgery in Breast Unit of AOUI Verona (MBC patients, n = 7; high-risk unaffected subjects, n = 19). We specifically examined the scores obtained by these subjects in the HADS questionnaire administered before and 6 months after the genetic testing for BRCA gene mutations. Results. Among the 17 unaffected men tested, 7 (41%) received a positive test (either BRCA1 or BRCA2 pathogenic variant) and 10 (59%) a negative test. Of the 9 MBC patients tested, only one subject received a positive test result. No significant differences were observed in mean scores, mean change from baseline to follow-up, either for those with T+ or T− test results. Discussion. Genetic testing for BRCA1/2 mutation was not associated in our sample with increased level of psychological distress as measured with HADS in a short-term evaluation. [ABSTRACT FROM AUTHOR]

In recent years, the temperature of processed olives in many olive-growing areas was often close to 30 °C, due to the global warming and an early harvesting period. Consequently, the new trends in the extraction process have to include the opportunity to cool the olives or olive paste before processing to obtain high quality EVOO. A tubular thermal exchanger was used for a rapid cooling treatment (CT) of olive paste after crushing. The results did not show a significant difference in the oil yield or any modifications in the legal parameters. The cooling process determined a significant improvement of phenolic compounds in all the three Italian cultivar EVOOs analyzed, whereas the volatile compounds showed a variability largely affected by the genetic origin of the olives with C 6 aldehydes that seem to be more stable than C 6 alcohols and esters. [ABSTRACT FROM AUTHOR]

The potential of a phenolic extract (PE) from olive vegetation water (OVW) to limit the negative effects of frying was tested after adding it at different concentrations to a refined olive oil (RO). Its efficacy was also compared to ROs containing butylated hydroxytoluene (BHT) and an extra virgin olive oil (EVOO) with a high polyphenol content. Analyses of the oils collected after 30 min, 1, 2, 4, 6, 8, 10 and 12 h of frying at 180 °C, demonstrated that degradation of the polyphenols was proportional to the original content; at a concentration of at least 400 mg/kg of polyphenols, PE was able to reduce oxidation of the tocopherols and the emission of low-molecular-weight aldehydes better than BHT and with similar results to the EVOO. In addition, secoiridoid oxidative compounds were examined by high-performance liquid chromatography/triple quadrupole tandem mass spectrometry with electrospray ionisation. [ABSTRACT FROM AUTHOR]

Abstract: Functional milk beverages (FMB100 and FMB200) fortified with phenolic compounds (100 and 200mg/l) extracted from olive vegetable water, and fermented with γ-amino butyric acid (GABA)-producing (Lactobacillus plantarum C48) and autochthonous human gastro-intestinal (Lactobacillus paracasei 15N) lactic acid bacteria were manufactured. A milk beverage (MB), without addition of phenolic compounds, was used as the control. Except for a longer latency phase of FMB200, the three beverages showed an almost similar kinetic of acidification, consumption of lactose and synthesis of lactic acid. Apart from the beverage, Lb. plantarum C48 showed a decrease of ca. Log 2.52–2.24cfu/ml during storage. The cell density of functional Lb. paracasei 15N remained always above the value of Log 8.0cfu/ml. During fermentation, the total concentration of free amino acids markedly increased without significant (P >0.05) differences between beverages. The concentration of GABA increased during fermentation and further storage (63.0±0.6–67.0±2.1mg/l) without significant (P >0.05) differences between beverages. After fermentation, FMB100 and FMB200 showed the same phenolic composition of the phenol extract from olive vegetable water but a different ratio between 3,4-DHPEA and 3,4-DHPEA-EDA. During storage, the concentrations of 3,4-DHPEA-EDA, p-HPEA and verbascoside of both FMB100 and FMB200 decreased. Only the concentration of 3,4-DHPEA increased. As shown by SPME-GC-MS analysis, diactetyl, acetoin and, especially, acetaldehyde were the main volatile compounds found. The concentration of phenolic compounds does not interfere with the volatile composition. Sensory analyses based on triangle and paired comparison tests showed that phenolic compounds at the concentrations of 100 or 200mg/l were suitable for addition to functional milk beverages. [Copyright &y& Elsevier]

Hydrophilic phenols are the most abundant natural antioxidants of virgin olive oil (VOO), in which, however, tocopherols and carotenes are also present. The prevalent classes of hydrophilic phenols found in VOO are phenolic alcohols and acids, flavonoids, lignans and secoiridoids. Among these substances the last two classes include the most concentrate phenols of VOO. Secoiridoids, like aglycone derivatives of oleuropein, demethyloleuropein and ligstroside, are present in olive fruit as most abundant VOO phenolic antioxidants. Several important biological properties (antioxidant, anti-inflammatory, chemopreventive and anti-cancer) and the characteristic pungent and bitter tasty properties have been attributed to VOO phenols. Relationships between polyphenols activities and their chemical structures are discussed in this paper. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Systemic sclerosis (SSc) is a disorder characterized by vascular damage and fibrosis of the skin and internal organs. Despite marked tissue hypoxia, there is no evidence of compensatory angiogenesis. The ability of mesenchymal stem cells (MSCs) to differentiate into endothelial cells was recently demonstrated. The aim of this study was to determine whether impaired differentiation of MSCs into endothelial cells in SSc might contribute to disease pathogenesis by decreasing endothelial repair. METHODS: MSCs obtained from 7 SSc patients and 15 healthy controls were characterized. The number of colony-forming unit-fibroblastoid colonies was determined. After culture in endothelial-specific medium, the endothelial-like MSC (EL-MSC) phenotype was assessed according to the surface expression of vascular endothelial growth factor receptors (VEGFRs). Senescence, chemoinvasion, and capillary morphogenesis studies were also performed. RESULTS: MSCs from SSc patients displayed the same phenotype and clonogenic activity as those from controls. In SSc MSCs, a decreased percentage of VEGFR-2+, CXCR4+, VEGFR-2+/CXCR4+ cells and early senescence was detected. After culturing, SSc EL-MSCs showed increased expression of VEGFR-1, VEGFR-2, and CXCR4, did not express CD31 or annexin V, and showed significantly decreased migration after specific stimuli. Moreover, the addition of VEGF and stromal cell-derived factor 1 to cultured SSc EL-MSCs increased their angiogenic potential less than that in controls. CONCLUSION: Our data strongly suggest that endothelial repair may be affected in SSc. The possibility that endothelial progenitor cells could be used to increase vessel growth in chronic ischemic tissues may open up new avenues in the treatment of vascular damage caused by SSc. [ABSTRACT FROM AUTHOR]

The article focuses on study which determined whether impaired differentiation of mesenchymal stem cells (MSCs) into endothelial cells in systemic sclerosis (SSc) might contribute to disease pathogenesis by decreasing endothelial repair. The study found that MSCs from SSc patients displayed the same phenotype and clonogenic activity as those from controls and the addition of vascular endothelial growth factor (VEGF) and stromal cell-derived factor 1 to cultured SSc EL-MSCs increased their angiogenic potential less than that in controls.

Vanin-1 is an epithelial ectoenzyme with pantetheinase activity and generating the amino-thiol cysteamine through the metabolism of pantothenic acid (vitamin B5). Here we show that Vanin-1-/- mice, which lack cysteamine in tissues, exhibit resistance to oxidative injury induced by whole-body γ-irradiation or paraquat. This protection is correlated with reduced apoptosis and inflammation and is reversed by treating mutant animals with cystamine. The better tolerance of the Vanin-1-/- mice is associated with an enhanced gamma-glutamylcysteine synthetase activity in liver, probably due to the absence of cysteamine and leading to elevated stores of glutathione (GSH), the most potent cellular antioxidant. Consequently, Vanin-1-/- mice maintain a more reducing environment in tissue after exposure to irradiation. In normal mice, we found a stress-induced biphasic expression of Vanin-1 regulated via antioxidant response elements in its promoter region. This process should finely tune the redox environment and thus change an early inflammatory process into a late tissue repair process. We propose Vanin-1 as a key molecule to regulate the GSH-dependent response to oxidative injury in tissue at the epithelial level. Therefore, Vanin/pantetheinase inhibitors could be useful for treatment of damage due to irradiation and pro-oxidant inducers. [ABSTRACT FROM AUTHOR]