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Aflatoxin constitutes a significant concern for food and feed safety, posing detrimental health risks to both animals and humans. This study aimed to examine the prevalence and concentration of aflatoxins in maize feed, total mixed ration, and wheat bran collected from specialized dairy farms and local markets in three major urban centers in eastern Ethiopia. A total of 180 feed samples were collected from September 2021 to January 2022 in Chiro town, Dire Dawa city, and Harar city. These samples underwent thorough extraction and immunoaffinity clean-up before aflatoxin analysis using HPLC/FLD. The results revealed that AFB1, AFB2, AFG1, AFG2, and TAF contamination was detected in 72.2%, 66.1%, 71.1%, 68.7%, and 82.8% of the feed samples, respectively. The corresponding mean levels of each aflatoxin were 28.15 ± 3.50, 3.3 ± 0.40, 19.87 ± 1.87, 2.7 ± 0.32, and 54.01 ± 4.72 µg/kg, respectively. The occurrence and levels of aflatoxin varied across different study sites and feed types. Notably, feeds from Dire Dawa city exhibited significantly higher mean levels of AFB1 (43.98 ± 5.3 µg/kg), AFB2 (5.69 ± 0.6 µg/kg), AFG1 (32.25 ± 2.7 µg/kg), and AFG2 (5.01 ± 0.5 µg/kg) than feeds from other urban centers did. Additionally, a significantly higher occurrence of AFB1 (29.4%) and AFG1 (28.3%) was detected in feed from Dire Dawa city. Similarly, the total mixed ration (TMR) displayed significantly higher levels of AFB1 (50.67 ± 5.2 µg/kg), AFB2 (4.74 ± 0.6 µg/kg), AFG1 (32.87 ± 2.6 µg/kg), and AFG2 (3.86 ± 0.5 µg/kg) compared to the other feed types. Moreover, a significantly higher occurrence of AFB1 (30.7%) and AFG1 (28.7%) was detected in the TMR. Furthermore, a moderate correlation was observed between the count of aflatoxigenic Aspergillus species and the levels of TAF in the feed samples. Overall, this study underscores the widespread presence of aflatoxin contamination in dairy feeds in eastern Ethiopia, highlighting the urgent need for stringent monitoring and mitigation measures to ensure food and feed safety, as well as public health. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. There is an increasing consumer desire for pasture-derived dairy products, as outdoor pasture-based feeding systems are perceived as a natural environment for animals. Despite this, the number of grazing animals globally has declined as a result of the higher milk yields achieved by indoor TMR feeding systems, in addition to the changing climatic conditions and lower grazing knowledge and infrastructure. This has led to the development of pasture-fed standards, stipulating the necessity of pasture and its minimum requirements as the primary feed source for products advertising such claims, with various requirements depending on the region for which it was produced. This work investigates the differences in the composition and techno-functional properties of butters produced from high, medium and no pasture allowance diets during early, mid, and late lactation. Butters were produced using milks collected from 3 feeding systems: outdoor pasture grazing (high pasture allowance); indoor TMR (no pasture allowance); and a partial mixed ration (medium pasture allowance) system, which involved outdoor pasture grazing during the day and indoor TMR feeding at night. Butters were manufactured during early, mid, and late lactation. Creams derived from TMR feeding systems exhibited the highest milk fat globule size. The fatty acid profiles of butters also differed significantly as a function of diet and could be readily discriminated by partial least squares analysis. The most important fatty acids in such an analysis, as indicated by their highest variable importance projection scores, were CLA C18:2 cis -9, trans -11 (rumenic acid), C16:1n-7 trans (trans -palmitoleic acid), C18:1 trans (elaidic acid), C18:3n-3 (α-linolenic acid), and C18:2n-6 (linoleic acid). Increasing pasture allowances resulted in reduced crystallization temperatures and hardness of butters and concurrently increasing the "yellow" color. Yellow color was strongly correlated with Raman peaks commonly associated with carotenoids. The milk fat globule size of cream decreased with advancing stage of lactation and churning time of cream was lowest in early lactation. Differences in the fatty acid and triglyceride contents of butter as a result of lactation and dietary effects demonstrated significant correlations with the hardness, rheological, melting, and crystallization profiles of the butters. This work highlighted the improved nutritional profile and functional properties of butter with increasing dietary pasture allowance, primarily as a result of increasing proportions of unsaturated fatty acids. Biomarkers of pasture feeding (response in milk proportionate to the pasture allowance) associated with the pasture-fed status of butters were also identified as a result of the significant changes in the fatty acid profile with increasing pasture allowance. This was achieved through the use of 3 authentic feeding systems with varying pasture allowances, commonly operated by farmers around the world and conducted across 3 stages of lactation. [Display omitted] [ABSTRACT FROM AUTHOR]

The present study was conducted to investigate the efficacy of different feed additives in TMR on in vitro total gas production and methane production. Total mixed ration was prepared by mixing of bajra straw, maize hay and concentrate in the ratio of 30:30:40. Feed additives included in TMR on DM basis were Monensin 30 mg/kg (T1), cellulase (10,00,000 IU/g) 0.5% (T2), xylanase (1,50,000 IU/g) 0.5% (T3), chitosan 1% (T4), sodium bicarbonate 1% (T5), magnesium oxide 1% (T6), combination of sodium bicarbonate + magnesium oxide 0.5% each (T7) and combination of cellulase + xylanase 0.5% each (T8). Rumen liquor was collected from two adult Surti goats each 2 h post-feeding of TMR with above additives. Result revealed that in vitro total gas production (mL/200 mg) was significantly (p<0.01) increased in TMRs supplemented with cellulase (28.39±0.51), xylanase (27.33±0.54) and its combination (32.92±0.62) as well as chitosan (26.82±0.99) as compared to control (22.08±0.56). In vitro methane production (%) was significantly (p<0.01) increased in TMRs supplemented with magnesium oxide (18.15±1.02) and its combination with sodium bicarbonate (17.91±0.15) as compared to control (16.59±0.19). Whereas, in vitro methane production (mL/200 mg & mL/100 mg) was significantly (p<0.05) increased in TMR supplemented with combination of cellulase and xylanase (5.13±0.13 & 2.56±0.06) as compared to control (3.66±0.05 & 1.83±0.02). While, ratios of in vitro methane production to fermentation parameters were significantly (p<0.01) improved in combination of cellulase and xylanase as compared to control. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate effects of forage inclusion and sources on performance, metabolism, and feeding behavior of dairy calves. Forty-eight Holstein calves were blocked and randomly assigned to 1 of 4 dietary treatments according to sex and BW at 28 d of life to determine the effects of feeding forage sources (ensiled and dry), with different quality on performance, metabolites, and behavior. Treatments consisted of a no-forage coarsely ground starter (CON); or total mixed ration (TMR) containing 7.5% on a dry matter (DM) basis of Tifton hay of either medium quality (MH) or low quality (LH); or 10% on a DM basis of corn silage (CS). During the first 28 d of life, all calves received 3 L of whole milk twice daily, a commercial pelleted starter and no forage, and water ad libitum. After that, the solid diet was changed to the respective dietary treatments. Calves were gradually weaned from 52 to 56 d of age, and followed for 14 d postweaning. Individual solid feed and milk intakes were recorded daily, and BW and metabolic indicators of intermediate metabolism were recorded weekly. Behavior was recorded, and the analysis was conducted on wk 7 (preweaning) and 10 (postweaning). Solid feed intake increased at wk 7 and 8 when MH, LH, and CS were included in TMR; the same results were observed postweaning. The diets did not affect the average daily gain and BW, but the feed efficiency increased with the CON diet. The β-hydroxybutyrate concentration was greater in calves receiving TMR-containing forage than CON diet. Furthermore, calves supplemented with forage had a greater rumination time. In conclusion, all forage sources included in the TMR showed feed intake and behavior benefits, reinforcing the need for fiber from forage in pre- and postweaning diets. [ABSTRACT FROM AUTHOR]

Simple Summary: High-producing dairy cows face an increased risk of subacute ruminal acidosis, which negatively affects the general health, feed intake, and the quantity and quality of milk produced. The aim of this study was to investigate the effect of calcareous marine algae (Lithotamium calcareum)-based rumen content buffer included in total mixed ration, fed to 34 high-producing, peak lactation Holstein dairy cows (group A, n = 17; group B, n = 17). It was hypothesized that through a rumen content buffering effect, buffer would improve feeding behavior, feed intake, rumen function, and performance. Differences between the experimental and control total mixed ration in most parameters under study (feed intake, rumen content acidity, feces composition, milk yield, and milk composition) were statistically insignificant. However, statistically significant differences were noted in the length of eating and chewing epizodes (feeding time; rumination time) between groups. Feed intake was in line with this, although the difference was only numerical and statistically insignificant. This may simply mean that the buffer effects takes some time to show but persists after the buffer withdrawal. One of the reasons for not achieving a significant improvement in other parameters might be that the cows were fed a typical, well-balanced ration that did not induce acidosis. The aim of the study was to investigate the effect of calcareous marine algae (Lithotamium calcareum)-based rumen content buffer (CMA) included in concentrated feed within total mixed ration (TMR), fed to 34 peak lactation (87–144 days in milk) Holstein dairy cows, randomized into two groups (group A, n = 17; group B, n = 17), wearing collars with accelerometers, and housed a in barn with automatic feed-weigh troughs. During the first phase P1, group A received TMR with CMA (TMR-E) and group B was fed TMR without the buffer (TMR-C). For P2, the treatments in the groups were exchanged. Feed intake, feeding time (FT), rumination time (RT), milk yield, milk composition, and rumen pH were measured by barn technologies, and rumen fluid and feces composition were analyzed in the laboratory. Differences between the TMR-E and TMR-C in most parameters under study were statistically insignificant, except overall FT and RT, which differed significantly between the groups. Group A, feeding at P1 by TMR-E, exhibited higher FT and RT than Group B (202 min/cow/day vs. 184 min/cow/day, and 486 min/cow/day vs. 428 min/cow/day, respectively). The RT significantly increased after switching from TMR-C to TMR-E. This implies that the buffer effect is delayed and persists after the withdrawal. In the group of cows that received control TMR without buffer in the first phase, RT and milk protein content increased significantly in the first week after the addition of buffer. [ABSTRACT FROM AUTHOR]

This study investigates the behavioral activities of grower Beetal kids fed in a conventional method-control group manner or given total mixed ration (TMR) (i.e. hay-based - T1 and green fodder-based - T2). Twenty-four weaned Beetal kids were enrolled and randomly distributed into 3 groups based on their body weight, sex, and age. Behavioral observations were recorded 30 min after giving experimental ration to the kids using the scan sampling method for 1 h, which inferred time spent eating normally, eating with forelegs on the cage, and eating with forelegs on the feeders; standing idle and urination were significantly higher in the control group than in the T1 and T2 groups (p < 0.01). Agonistic activities like bunting were numerically higher for Beetal kids fed with hay-based TMR, followed by the T2 and control groups (not significantly affected). Grooming using other inanimate objects in the pen (p < 0.01) and pawing the ground (p < 0.05) were found to be significantly higher in the T1 group than in the other groups. Animals in the hay-based TMR-fed group also spent significantly (p < 0.01) more time ruminating while standing compared to the control and T2 groups. During normal sitting activity, the variations were statistically higher (p < 0.05) for the green fodder-based TMR group than the other groups. The haybased TMR-fed group consumed the offered feed with more relish, ate more quickly, and showed agonistic activities among mates in their cages or pens. [ABSTRACT FROM AUTHOR]

Simple Summary: Preweaning nutrition plays a crucial role in the growth of dairy calves, determining their future productivity and overall health. Researchers are still studying the most appropriate dietary strategies in the preweaning and early post-weaning periods. In particular, the effect of different levels of forage inclusion in the diet of young cattle is not yet clear. Our study evaluated the effects of starter restriction and ad libitum inclusion of the total mixed ration on intake, growth performance, rumination time, and health status of Holstein dairy calves during preweaning and in the immediate post-weaning period. The results obtained from the present study indicate that providing TMR (high fiber content, with or without concentrate restriction) does not impair growth performance and health of calves, and at the same time it leads to a greater time spent for ruminating when calves are weaned. The latter indicates a better rumen development and likely a better rumen fermentation efficiency in post-weaning. The aim of the current study was to evaluate the effect of the starter restriction and of the ad libitum TMR (total mixed ration) inclusion on intake, growth performance, rumination time (RT), and health condition of Holstein dairy calves during weaning. We randomly assigned thirty female Holstein calves (with an average weight of 38.5 ± 1.96 kg at birth) to one of three treatments. From 21 days of age, the calves were fed one of three treatments as follows: a control diet (CTR) with an ad libitum calf starter but without TMR; Treatment 1 diet (TRT1) with both an ad libitum calf starter and ad libitum TMR; Treatment 2 diet (TRT2) with ad libitum TMR and a restricted amount of a calf starter (50% of the intake recorder in the control group day by day). Calves in the TRT2 group, between 56 and 63 days of age, had a lower body weight (80.1; 79.5; 75.6 kg for the CTR, TRT1, and TRT2 groups, respectively) compared with CTR and TRT1 calves. This outcome is ascribed to the average daily gain (0.759; 0.913; 0.508 kg/day for the CTR, TRT1, and TRT2 groups, respectively), resulting also in TRT2 being lower than CTR or TRT1 calves. The inclusion of ad libitum TMR increased the rumination time, especially after weaning (15.28 min/h, 18.38 min/h, and 18.95 min/h for the CTR, TRT1, and TRT2 groups, respectively). Concerning the rumen metabolism and inflammometabolic response, overall, no differences were observed between the three dietary treatments. In conclusion, the results indicated that a TMR could partially replace a calf starter in weaning dairy calves, since neither growth performance nor health status were impaired. In addition, providing TMR (with or without concentrate restriction) led to a better rumen development and likely a better rumen fermentation efficiency in post-weaning. [ABSTRACT FROM AUTHOR]

Simple Summary: Livestock farming is experiencing growing levels of automation due to the high number of repetitive tasks requiring little skill and high workloads. Automatic feeding systems for ruminants represent a valuable option for farmers to reduce their daily workload and increase animal welfare and production quality. The farmers' perspective matched the outcome of the studies on the benefits of automatic feeding system adoption (lower energy requirements, lower feed waste, and increased animal welfare). However, such benefits only exist if thorough economic and structural planning considers all of the farming site's features to exploit the feeding robots' flexibility at its best. We suggest that automatic feeding systems may be fundamental to the involvement of younger generations in animal farming and increasing the inclusivity of such an activity with the subsequent fostering of female entrepreneurship, always complying with good feeding practices. Automation reduces the impact of farming on climate change and helps farmers adapt to its financial impact. Automatic feeding systems (AFSs) increase the ruminant's feeding precision and ease operators' workload. Such systems exist on a spectrum, requiring varied levels of operator support and installation complexity. A recent survey on farmers pointed out that those already running an AFS and those willing to buy one appreciate its ease of use, the resulting animal welfare, and the resulting overall benefits (increased production, farm profitability, and reduced feed waste). Whether technologically complex or not, studies have confirmed the benefits that farmers perceive to be underlying the remarkable reduction in the environmental impact of feeding operations (AFSs are electrically powered), the increase in animal welfare resulting from reduced conflicts around accessing the feed bunk, and the constant availability of fresh feed. However, their introduction should follow accurate and holistic structural and economic planning for existing and newly built facilities. The availability of public subsidies plays an essential role in pushing farmers to adopt the most modern digital technologies, whose benefits may even increase when farmers couple them with interconnected sensors to monitor animals' physiological states. [ABSTRACT FROM AUTHOR]

The objective of this study was to examine the impact of increasing proportions of grazed pasture in the diet on the composition, quality, and functionality of bovine milk across a full lactation. Fifty-four spring-calving cows were randomly assigned to 1 of 3 groups (n = 18), blocked on the basis of mean calving date (February 15, 2020 ± 0.8 d), pre-experimental daily milk yield (24.70 ± 3.70 kg), milk solids yield (2.30 ± 0.27 kg), lactation number (3.10 ± 0.13), and economic breeding index (182 ± 19). Raw milk samples were obtained weekly from each group between March and November 2020. Group 1 (GRS) consumed perennial ryegrass and was supplemented with 5% concentrates (dry matter basis); group 2 was maintained indoors and consumed a total mixed ration (TMR) diet consisting of maize silage, grass silage, and concentrates; and group 3 consumed a partial mixed ration diet (PMR), rotating between perennial ryegrass during the day and indoor TMR feeding at night. Raw milk samples consisted of a pooled morning and evening milking and were analyzed for gross composition, free amino acids, fatty acid composition, heat coagulation time, color, fat globule size, and pH. The TMR milks had a significantly higher total solids, lactose, protein, and whey protein as a proportion of protein content compared with both GRS and PMR milks. The GRS milks demonstrated a significantly lower somatic cell count (SCC), but a significantly higher pH and b*-value than both TMR and PMR milks. The PMR milks exhibited significantly lower total solids and fat content, but also demonstrated significantly higher SCC and total free amino acid content compared with GRS and TMR. Partial least squares discriminant analysis of fatty acid profiles displayed a distinct separation between GRS and TMR samples, while PMR displayed an overlap between both GRS and TMR groupings. Variable importance in projection analysis identified conjugated linoleic acid cis -9, trans -11, C18:2n-6 cis , C18:3n-3, C11:0, and C18:2n-6 trans as the largest contributors to the variation between the diets. Milk fats derived from GRS diets exhibited the highest proportion of unsaturated fats and higher unsaturation, health-promoting, and desaturase indices. The lowest proportions of saturated fats and the lowest atherogenic index were also exhibited by GRS-derived milk fats. This work highlights the positive influence of grass-fed milk for human consumption through its more nutritionally beneficial fatty acid profile, despite the highest milk solid percentages derived from TMR feeding systems. Furthermore, this study demonstrates the proportional response of previously highlighted biomarkers of pasture feeding to the proportion of pasture in the cow's diet. [Display omitted] [ABSTRACT FROM AUTHOR]

Simple Summary: As the price of imported corn rises due to environmental issues and the growing demand for bio-ethanol production, animal nutritionists are actively seeking alternative energy sources to corn. While previous research has shown that rice grain provides a comparable nutritional value to corn, it is noted that most of the studies on rice grain as a starch source for ruminants have been conducted on dairy cattle. The objective of this study is to assess the effects of total mixed ration (TMR) containing rice grains on the growth performance, blood metabolites, rumen fermentation, and rumen microbial community in fattening Hanwoo steers. Our results demonstrated that rice grain had no adverse effects on the growth performance of Hanwoo steers, rumen fermentation characteristics, and blood metabolites. In conclusion, this study suggests that utilizing rice grains as the primary starch source in TMR may serve as an alternative option for fattening Hanwoo steers. This study aimed to assess the influence of rice grain in the total mixed ration (TMR) on the growth performance, blood metabolites, rumen fermentation, and rumen microbial community of fattening Hanwoo steers. Two experimental diets were prepared: (i) a TMR containing 33% dry matter (DM) corn grains (Corn TMR) and (ii) a TMR containing 33% DM rice grains (Rice TMR). Twenty-two Hanwoo steers (body weight [BW], 498 ± 32 kg; months, 17 ± 0.5) were distributed into two treatment groups in a completely randomized block design according to BW. The Rice TMR group had a higher final BW and DM intake (DMI) compared to those in the Corn TMR group (p < 0.01). However, no difference was observed in the average daily gain (ADG) and feed conversion ratio (FCR) between the two treatments. For the rumen fermentation parameters, the molar portion of butyrate in the Rice TMR was higher than in the Corn TMR (p < 0.01). Streptococcus bovis tended to be higher in the Rice TMR (p = 0.09). The results of this study suggest that using rice grain as the primary starch source in TMRs may be an alternative option for fattening Hanwoo steers. [ABSTRACT FROM AUTHOR]

Simple Summary: Fermented total mixed ration (FTMR) is a method used to ferment feed under anaerobic conditions in a tightly sealed container, with the potential to enhance nutrient utilization and extend the shelf life of feed. We found that the loss of aerobic stability may be associated with the dominant yeast species found in FTMR; however, there is no discernible relationship between yeast counts and the loss of aerobic stability. However, the replacement of soybean meal (SBM) with cottonseed meal (CSM) and rapeseed meal (RSM) in total mixed rations (TMRs) by fermented TMR has an effect on the degradation of anti-nutritional factors. In order to explore the effects of fermentation and aerobic exposure to FTMR on nutrients and anti-nutritional factors, this study aimed to ascertain the impact of B. clausii and S. cariocanus inoculations on TMR fermentation quality, aerobic stability, anti-nutritional factors, and in situ rumen-degradation characteristics' variables. We found that TMRs fermented with inoculations of B. clausii and S. cariocanus improved the fermentation quality and nutrient composition, decreased the anti-nutritional factor content, and, thus, achieved detoxification. Meanwhile, the effective disappearance of nutrients in the rumen was increased. The purpose of this experiment was to evaluate changes in fermentation quality, chemical composition, aerobic stability, anti-nutritional factors, and in situ disappearance characteristics of various protein-based total mixed rations. Soybean meal (control, non-fermented), fermented cottonseed meal (F-CSM), and fermented rapeseed meal (F-RSM) group were used to prepare the TMRs with corn, whole-plant corn silage, corn stalks, wheat bran, and premix. The test groups were inoculated at 50% moisture with Bacillus clausii and Saccharomyces cariocanus and stored aerobically for 60 h. The nylon-bag method was used to measure and study the rumen's nutrient degradation. The pH of all TMRs after 48 h of air exposure was below 4.8, whereas that of the F-CSM and control and F-RSM groups increased to 5.0 and >7.0, respectively. After 8 h of aerobic exposure, the temperatures of all groups significantly increased, and 56 h later, they were 2 °C higher than the surrounding air. The lactic acid concentration in the F-CSM and F-RSM groups increased after 12 h of aerobic exposure and then decreased. The acetic acid concentrations in the fermented groups decreased significantly with the increasing air-exposure time. The yeast population of the TMRs increased to more than 8.0 log10 CFU/g before 72 h of air exposure, followed by a decrease in the population (5.0 log10 CFU/g). After fermentation, the free gossypol (FG) concentration in F-CSM decreased by half and did not change significantly during the air-exposure period. Fermentation with probiotics also reduced the F-RSM's glucosinolate concentration, resulting in a more than 50% detoxification rate. Compared with the F-CSM and F-RSM groups, the effective degradation rates of nutrients in the control group were the lowest, and the dry matter (DM), crude protein (CP), natural detergent fiber (NDF), and acid detergent fiber (ADF) all degraded effectively at rates of 28.4%, 34.5%, 27.8%, and 22.8%, respectively. Fermentation with B. clausii and S. cariocanus could improve the fermentation quality and nutrient composition, decrease the anti-nutritional factor, and increase nutrient degradation of the TMR with cottonseed meal or rapeseed meal as the main protein source, thus achieving detoxification. [ABSTRACT FROM AUTHOR]

Aiming at the problems of low quality of milk production and low feeding efficiency caused by uneven stirring of components in total mixed ration (TMR), a vertical screw stirring device for feeding dairy goat was designed. In this study, based on the material properties of TMR for dairy goats, the pitch (S), bottom inclination angle (Z), and rotational speed of the vertical screw (n) and the sidewall angle (β) and loading rate (L) of the mixing chamber were simulated and analysed by the discrete element method, the optimal parameter combination for the system were determined. Furthermore, a testbed of the stirring device was built in accordance with simulation analysis conclusions, verifying the working performance of the device. Results demonstrated that when S = 300 mm, Z = 70°, β = 105°, n = 60 r/min, L = 70 %, the CV of mixing uniformity is 12.95 % and mixing power is 1.35 kW. A test showed that the CV of mixing uniformity was 13.03 %, and that of mixing power was 1.41 kW. These results are basically consistent with the simulation analysis results. The proposed results provide evidence for the design of feeding devices for dairy goats. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effects of the inclusion of whole-plant corn silage (WPCS) in a starter or total mixed ration (TMR) on growth, blood metabolites, ruminal fermentation, and microbial community in preweaning dairy calves. A total of 45 healthy dairy calves were blocked by date of birth and randomly assigned to 1 of 3 treatments: 100% calf starter (CONS), a mix of 85% calf starter and 15% WPCS [dry matter (DM) basis; CSCS], or 100% WPCS-based lactation TMR (CTMR). Pasteurized normal milk was fed to all the animals under the same regimen. The experiment ran from when the calves were 2 d old to weaning at 63 d. Milk and feed intakes were recorded daily. Growth performance data and blood samples were collected on wk 3, 5, 7, and 9 of the experiment. Rumen fluid was sampled at 40 and 60 d. The 3 treatments had different particle size fractions. The CSCS group had greater medium fraction (<19 mm, >8 mm) and particles retained on 8-mm sieves than the other 2 groups, whereas the CTMR group had the greatest long (>19 mm) and fine (<4 mm) fractions and physically effective neutral detergent fiber (NDF) on 8- and 4-mm sieves, but had the smallest short fraction (<8 mm, >4 mm) and particles retained on 4-mm sieves. The 24-h in vitro digestibility of DM, crude protein (CP), NDF, and acid detergent fiber (ADF) were decreased in order by the CONS, CSCS, and CTMR groups. Compared with the CONS group, the digestibility of ether extract (EE) was lower in the CSCS and CTMR groups, whereas the digestibility of starch was similar among treatments. During the experimental period, the DM, CP, and metabolizable energy intakes from milk, solid feed, and total feed were not affected by treatments. The NDF, ADF, and EE intakes and potentially digestible intakes were greater in the CTMR group than in the other 2 groups. With the exception that body barrel was greater for calves fed CSCS, growth parameters and blood metabolites were similar among treatments. Compared with the CSCS group, the CTMR group had greater rumen pH and total volatile fatty acids, propionate, and isovalerate concentrations, but a lower acetate:propionate ratio. The CTMR group had greater relative abundances of some cellulolytic bacteria (Rikenellaceae RC9 gut group, Christensenellaceae R7, Ruminococcaceae NK4A214, Ruminococcaceae UCG, Ruminococcus , and Erysipelotrichaceae UCG) in the rumen, which may be beneficial for the early acquisition of specific adult-associated microorganisms. In summary, a WPCS-based lactation TMR, but not the WPCS-included starter, had the potential to be an alternative starter in preweaning calves without having significant adverse effects. These findings provide theoretical and practical implications for the rational application of TMR in the early life of dairy calves. [ABSTRACT FROM AUTHOR]

Simple Summary: An investigation of poor aerobic stability in high-moisture total mixed rations (TMRs) for livestock feed was conducted in this study. TMR quality factors were discovered, and prospective approaches to increase its fermentation properties and overall stability were investigated. Using infrared thermography (IRT) measurements, it was suggested that dairy cow feeding methods can be optimized in the field by identifying portions with a higher center temperature and maximum temperature difference values. According to the findings, there is a significant potential for IRT to be used in feed management and preservation processes in the future, resulting in higher levels of productivity for livestock. A major objective of this study is to identify factors influencing the quality of high-moisture total mixed rations (TMRs) for livestock feed and explore possible manipulations that can enhance their fermentation characteristics and stability in order to address the problem of poor aerobic stability. Therefore, the current study utilized infrared thermography (IRT) to assess the aerobic stability of water-added TMRs in the feed bunker. By manipulating the moisture content of freshly prepared TMRs at four different levels through water addition and subjecting it to storage at two consistent temperatures, significant correlations between IRT values (center temperature (CT) and maximum temperature difference (MTD)) and key parameters such as lactic acid bacteria, water-soluble carbohydrates, and TMR pH were established. The first and second principal components together accounted for 44.3% of the variation, with the first component's load influenced by IRT parameters, fermentation characteristics, and air exposure times, while the second component's load was influenced by dry matter content and lactic acid concentration. The results of these studies indicate the possibility that feeding methods can be optimized by identifying portions with higher CT or MTD data using IRT measurements just before feeding dairy cows in the field. As a result, increasing the use of IRT in feed management and preservation processes is projected to have a positive impact on animal productivity in the future. [ABSTRACT FROM AUTHOR]

Simple Summary: Pasture-based systems have advantages compared to confined systems, such as a higher proportion of beneficial milk fatty acids for their consumers. However, in mixed systems (grazing + total mixed ration), cows are more exposed to external climatic conditions. Due to climate change (high temperature or heavy rains), cows spend more time in confinement facilities where supplementation is offered, mainly in intensive mixed systems (high stocking rate). Therefore, the conditions of the facility during their confinement acquire great importance. To our knowledge, no studies have evaluated how the different facility conditions during confinement in a mixed system affect the fatty acid profiles in milk and cheese. The objective of this study was to compare the fatty acid profiles between mixed systems with different conditions during confinement (compost-bedded pack barns vs. outdoor soil-bedded pen) and mixed systems and confinement systems (100% total mixed ration) in a compost-bedded pack barns. In conclusion, the compost-bedded pack barns during confinement in a mixed system ensued a better milk quality (a higher percentage of Omega 3 (n-3) and C18:3 in the milk) compared to the outdoor soil-bedded pen. However, the fatty acid profiles in the milk, pooled milk (MilkP), and cheese were affected to a greater extent by the feeding management than by controlling the environment during the confinement. The diet of dairy cows influences the fatty acid (FA) profiles of their milk and cheese, but how these are affected by different conditions during confinement in a mixed system (MS:grazing + total mixed ration:TMR) is not known. The aim of this study was to compare the FAs of the milk and cheese from MS in a compost-bedded pack barns (CB-GRZ) versus an outdoor soil-bedded pen (OD-GRZ) during confinement, and with a confinement system (100%TMR) in a compost-bedded pack barns (CB-TMR). Individual milk samples (n = 12 cows/group), cheese, and pooled milk (MilkP) samples were collected. The saturated FA percentages in the milk and the omega 6/omega 3 ratio in the MilkP and cheese were greater for the CB-TMR (p < 0.0001), while the unsaturated and monounsaturated FA percentages in the milk were lower for the CB-TMR than the MS (p < 0.001). The milk n-3, C18:3, and conjugated linoleic acid percentages were lower for the CB-TMR than the MS (p < 0.001). The milk n-3 and C18:3 were higher for the CB-GRZ than the OD-GRZ (p < 0.01), but no differences were observed between the MS in the MilkP and cheese. In conclusion, CB-GRZ cows during confinement produced better quality milk compared to OD-GRZ cows. However, the FA profiles of the milk, MilkP, and cheese were affected to a greater extent by the feeding management than by the conditions during confinement. [ABSTRACT FROM AUTHOR]

Simple Summary: Methane and volatile fatty acids are important products of rumen fermentation in dairy cows. Quantitative research on them is of great significance for environmental protection and animal production. The aim of this study was to develop a prediction model using the stacking ensemble learning method and predict the production of rumen fermentation products based on the nutrient level of the diet. The results show that the stacking model has good performance in terms of prediction accuracy. The model proposed in this study can be used as a guideline to optimize diet compositions and improve feeding efficiency. Volatile fatty acids (VFAs) and methane are the main products of rumen fermentation. Quantitative studies of rumen fermentation parameters can be performed using in vitro techniques and machine learning methods. The currently proposed models suffer from poor generalization ability due to the small number of samples. In this study, a prediction model for rumen fermentation parameters (methane, acetic acid (AA), and propionic acid (PA)) of dairy cows is established using the stacking ensemble learning method and in vitro techniques. Four factors related to the nutrient level of total mixed rations (TMRs) are selected as inputs to the model: neutral detergent fiber (NDF), acid detergent fiber (ADF), crude protein (CP), and dry matter (DM). The comparison of the prediction results of the stacking model and base learners shows that the stacking ensemble learning method has better prediction results for rumen methane (coefficient of determination ( R 2 ) = 0.928, root mean square error (RMSE) = 0.968 mL/g), AA ( R 2 = 0.888, RMSE = 1.975 mmol/L) and PA ( R 2 = 0.924, RMSE = 0.74 mmol/L). And the stacking model simulates the variation of methane and VFAs in relation to the dietary fiber content. To demonstrate the robustness of the model in the case of small samples, an independent validation experiment was conducted. The stacking model successfully simulated the transition of rumen fermentation type and the change of methane content under different concentrate-to-forage (C:F) ratios of TMR. These results suggest that the rumen fermentation parameter prediction model can be used as a decision-making basis for the optimization of dairy cow diet compositions, rapid screening of methane emission reduction, feed beneficial to dairy cow health, and improvement of feed utilization. [ABSTRACT FROM AUTHOR]

The work aimed to evaluate the effect of different levels of soy sauce residue (SSR) replacement on chemical compositions, fermentation quality and in vitro digestibility of total mixed ration (TMR) silages. The experiment followed a completely randomised design, and four treatments were designed according to different mixing ration of SSR, Napier grass and concentrate (based on fresh weight): (1) 65% Napier grass + 35% concentrate (control); (2)15% SSR + 55% Napier grass + 30% concentrate (SS15); (3) 25% SSR + 50% Napier grass + 25% concentrate (SS25); (4) 35% SSR + 45% Napier grass + 20% concentrate (SS35). TMR was ensiled in a laboratory silo (10 L) after mixing. All silos were opened after 60 days of ensiling and then were sampled for chemical compositions, fermentation quality and in vitro digestibility analysis. All TMR silages were preserved well, as indicated by the low butyric acid (BA; <1.60 g/kg dry matter), ammonia nitrogen (NH3-N; <50.0 g/kg total nitrogen) contents, and the high V-Score value (>80.0). However, SS25 and SS35 significantly (P < 0.05) decreased the BA, NH3-N, ethanol contents and yeast counts, and significantly (P < 0.05) increased the V-Score value compared with the control. SS35 significantly (P < 0.05) increased 72 h cumulative gas production (GP72), in vitro dry matter digestibility (IVDMD) and crude protein digestibility (IVCPD) compared with control and SS15. There was no significant (P > 0.05) difference in vitro neutral detergent fibre digestibility (IVNDFD) among all TMR silages. In conclusion, SS35 replacement was recommended for TMR silage making. Ensiling is an effective method to utilise soy sauce residue. Soy sauce residue replacement had no unfavourable effect on the fermentation quality of the total mixed ration. 35% soy sauce residue replacement improved the in vitro digestibility of total mixed ration silages. [ABSTRACT FROM AUTHOR]

Chemical additives have been widely used to restrain the growth of undesirable microorganisms, and they were ideal additives to well preserve silages. The work aimed at evaluating the effects of four chemical additives on fermentation quality, aerobic stability and in vitro ruminal digestibility of the fermented total mixed ration (FTMR). The TMR was treated with: (1) no additive (control); (2) sodium diacetate; (3) calcium propionate; (4) sodium benzoate (SB); (5) potassium sorbate (PS) on a fresh weight (FW) basis. After 60 days of ensiling, the silos were opened and sampled to determine fermentation quality, in vitro rumen parameters, and then the 60-day FTMR were subjected to a 9-day aerobic stability test. Chemical additives significantly (p < 0.05) increased dry matter, crude protein, and water-soluble carbohydrates contents, and decreased ammonia nitrogen, ethanol contents, aerobic bacteria and yeast counts after 60 days of ensiling. During aerobic exposure, chemical additives obviously improved aerobic stability as indicated by higher lactic acid, water-soluble carbohydrates contents and lactic acid bacteria counts, and lower pH, ethanol, ammonia nitrogen contents, aerobic bacteria and yeast counts. Treatments with SB and PS had higher (p < 0.05) lactic acid content, and lower (p < 0.05) pH, ammonia nitrogen content, aerobic bacteria and yeast counts than other FTMR. After 72 h of incubation, chemical additives significantly (p < 0.05) increased cumulative gas production, in vitro digestibility of dry matter and crude protein. The SB and PS-treated FTMR had higher (p < 0.05) cumulative gas production and in vitro dry matter digestibility than other FTMR. These results indicated that SB and PS were more effective to improve aerobic stability and in vitro ruminal parameters, and they were recommended as optimal additives for FTMR. Four chemical additives were served as additives for fermentation total mixed ration Chemical additives markedly improved fermentation quality, aerobic stability and in vitro ruminal parameters Sodium benzoate and potassium sorbate were optimal for improving aerobic stability and in vitro parameters [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the predictive ability of NIRS for the estimation of the chemical composition and organic matter digestibility (OMD) of total mixed rations (TMR) for dairy cows. Moreover, herein, empirical equations based on chemical parameters were developed for calculating the OMD. Samples were collected from Galician dairy farms and were scanned in duplicate a Foss NIRSystem 6500 monochromator (1100-2500 nm). The predictive ability of NIRS models was evaluated according to the coefficient of determination in external validation ( r 2). High r 2 (equal to or higher than 0.90) was shown in predicting chemical composition, however, the estimation of OMD was acceptable ( r 2 = 0.83). The ability of the NIRS models for estimating the chemical composition was considered excellent, with the ratio performance deviation of external validation ( R P D) higher than 3.0, allowing for quantitative predictions. The estimation of the OMD value by NIRS was considered acceptable ( R P D = 2.5), and led to reduction in the standard error of external validation, in comparison of the best empirical model based on the chemical composition of samples (from ± 2.06% to ± 1.89%). Therefore, this study demonstrated that the NIRS is an effective technology for the rapid and precise nutritional evaluation of TMR in Galicia. [ABSTRACT FROM AUTHOR]

This study aims to explore the presence of mycotoxins in total mixed rations (TMR) employed for feeding dairy cattle and their potential carry-over to milk. A total of 87 TRM samples were collected in farms from the north of Portugal from 2019 to 2022. A method based on a QuEChERS extraction followed by UHPLC-MS/MS detection was employed for sample analysis. The method was in-house validated in terms of matrix effect, linearity, sensitivity, accuracy and precision. The most frequently detected regulated toxins were fumonisins, with a positivity rate of 74%, while deoxynivalenol and zearalenone were found in approximately 35% of the samples. Among the emerging toxins, beauvericin and enniatins exhibited the highest detection rates. In addition, milk samples were collected from 21 farms, providing insights into the carry-over of these toxins and roquefortine to milk, with an estimated rate ranging between 2% and 10%. • A total of 87 TRM samples were collected from 2019 to 2022 • An UHPLC-MS/MS detection method was employed for sample analysis • Roquefortine, beauvericin and enniatins are carried-over to milk [ABSTRACT FROM AUTHOR]

The aim of the present study was to evaluate the effects of replacing barley grain with heated potato slice wastes (HPSW) or French fries' wastes (FFW) on dry matter intake and production performance of lactating Holstein dairy cows. Twenty-four Holstein dairy cows (body weight: 639 ± 20.3 kg, parity: 3.0 ± 0.21, days in milk: 123 ± 11.7, raw milk yield: 41.5 ± 2.86 kg/d) were randomly assigned to 3 treatments: 1) Control: diet containing 204 g/kg of dry matter (DM) barley grain as the main source of non-forage carbohydrates, 2) HPSW: diet containing 104 g/kg DM barley grain and 100 g/kg DM HPSW, 3) FFW: diet containing 104 g/kg DM barley grain and 100 g/kg DM FFW. The results showed that the frying process of potato increased the fatty acid content from 4.9 to 198 g/kg DM. The replacement of barley grain with HPSW or FFW decreased dry matter intake significantly (P=0.02) and values were 24.6, 23.3, and 23.8 kg/d for control, HPSW and FFW respectively. Also, results revealed higher energy corrected milk yield for HPSW and FFW (42.9 and 43.9 kg/d respectively) than control (40.6 kg/d). When compared to control, feeding HPSW and FFW enhanced feed efficiencies (P<0.01) and the values were 1.61, 1.75, and 1.77 for control, HPSW and FFW respectively. As barley grain was replaced with potato co-products, the proportion of nitrogen intake excreted in the urine decreased (P<0.01), with values of 312, 327, and 380 g/kg for HPSW, FFW and control respectively. Also, milk urea nitrogen and the ratio of urine nitrogen to intake nitrogen were lower for HPSW and FFW than in the control, indicating a more efficient utilization of dietary crude protein by dairy cows in treatments containing potato co-products. The inclusion of fried and cooked potato co-products increased (P<0.01) the physically effective neutral detergent fiber of diets measured at zero time from 122 for control to 181 and 172 g/kg DM for HPSW and FFW respectively. Consequently, rumination time (min/day) increased from 417 for control to 527 and 555 for HPSW and FFW respectively, which confirmed the positive effects of heated potato processing co-products on dietary physical properties. In conclusion, the results of the current study showed that replacement of barley grain with fried and cooked potato co-products improved diet physical properties and production performance of Holstein lactating dairy cows. • Agro-food by-products can be used in the diet of lactating dairy cows. • Fried and cooked potato co-products are classified as high-energy and low-fiber feed. • Heated potato co-products improve milk production and feed efficiency in dairy cows. • Fried and cooked potato co-products increase the diets particle size and rumination time. [ABSTRACT FROM AUTHOR]

Context. Oil palm frond (OPF) is abundantly available throughout Southeast Asia and is a good source of forage for feedlot cattle, particularly during the dry and monsoon seasons when other forage options are limited. However, the use of OPF in ruminants feed is constrained by its complex fibrous structure and low digestibility. Aims. The aim of this study was to investigate the impact of supplementation with bypass fat on growth, meat quality and economic returns in smallholder feedlot systems where Napier grass is replaced with OPF. Methods. Sixteen Brahman · Charolais crossbred steers, 23 2.0 months old and with initial bodyweight of 425 59.9 kg (mean s.e.), were randomly allocated in a 2 · 2 factorial randomised complete-block design experiment with the following dietary treatments: (i) fresh chopped Napier grass-based total mixed ration (TMR; Napier grass-fat), (ii) Napier grass-based TMR + 5% bypass fat (Napier grass+fat), (iii) OPF-based TMR (OPF-fat) and (iv) OPF-based TMR + 5% bypass fat (OPF+fat). Feed intake, digestibility of the diets and average daily gain were measured. The cattle were slaughtered to determine carcass dressing percentage and meat quality. Costs and return of fattening cattle were estimated. Key results. Despite higher intake, cattle fed OPF-fat had a lower bodyweight gain than did cattle fed grass-based diets due to lower digestibility. Bypass-fat supplementation increased the bodyweigh of cattle fed OPF but not of cattle fed Napier grass. Fat supplementation enhanced colour, backfat thickness, and fat content of meat in both the Napier grass- and OPF-based diets. However, replacing Napier grass with OPF reduced the net profit of smallholder feedlot systems, even with fat supplementation. Conclusion. While fat supplementation increased liveweight gain and enhanced some aspects of meat quality, the increased feeding cost reduced net profit. Therefore, supplementation of OPF with bypass fat is not recommended for smallholder feedlots in developing countries. Implications. Appropriate technology to reduce the feeding cost of OPF needs to be developed to make it an economically viable option for smallholder farmers. [ABSTRACT FROM AUTHOR]

Simple Summary: Livestock accounts for an estimated 80% of total agricultural greenhouse gas emissions, making abatement of greenhouse gas emissions from livestock a high-priority challenge facing animal nutritionists. Mitigating greenhouse gases in ruminants without reducing animal production is desirable both as a strategy to reduce global greenhouse gas emissions and as a way of improving dietary feed efficiency. The inclusion of feed additives in the diets of ruminants can reduce energy losses as methane, which typically reduces animal performance and contributes to greenhouse gas emissions. The present study evaluated the abatement potential of nine essential oil blends to mitigate greenhouse gas emissions. The inclusion of the blends resulted in a reduction in greenhouse gas emissions and in vitro apparent dry matter digestibility with higher values noted for the control treatment. A similar trend was noted for in vitro truly dry matter digestibility with higher values noted in the control treatment. The efficiency of microbial production was greater for the blends. The inclusion of the blends affected the total and molar proportion of volatile fatty acid concentrations. Overall, inclusion of the blends modified the rumen function resulting in improved efficiency of microbial production. The current study evaluated nine essential oil blends (EOBs) for their effects on ruminal in vitro dry matter digestibility (IVDMD), efficiency of microbial production, total short-chain fatty acid concentration (SCFA), total gas, and greenhouse gas (GHG) emissions using two dietary substrates (high forage and high concentrate). The study was arranged as a 2 × 2 × 9 + 1 factorial design to evaluate the effects of the nine EOBs on the two dietary substrates at two time points (6 and 24 h). The inclusion levels of the EOBs were 0 µL (control) and 100 µL with three laboratory replicates. Substrate × EOBs × time interactions were not significant (p > 0.05) for total gas and greenhouse gas emissions. The inclusion of EOBs in the diets resulted in a reduction (p < 0.001) in GHG emissions, except for EOB1 and EOB8 in the high concentrate diet at 6 h and for EOB8 in the high forage diet at 24 h of incubation. Diet type had no effect on apparent IVDMD (IVADMD) whereas the inclusion of EOBs reduced (p < 0.05) IVADMD with higher values noted for the control treatment. The efficiency of microbial production was greater (p < 0.001) for EOB treatments except for EOB1 inclusion in the high forage diet. The inclusion of EOBs affected (p < 0.001) the total and molar proportion of volatile fatty acid concentrations. Overall, the inclusion of the EOBs modified the rumen function resulting in improved efficiency of microbial production. Both the apparent and truly degraded DM was reduced in the EOB treatments. The inclusion of EOBs also resulted in reduced GHG emissions in both diets, except for EOB8 in the high forage diet which was slightly higher than the control treatment. [ABSTRACT FROM AUTHOR]

Aims: To obtain insights into how bacterial and fungal microbiota and fermentation products composition are affected by storage temperature for TMR silage, which can be manufactured year‐round. Methods and results: TMR silage was stored at 10°C, 25°C, ambient temperature (AT; 20–35°C) and 40°C. Lactic acid production was delayed when stored at 10°C, and acid production stagnated after 2 weeks when stored at 40°C. The patterns of acetic acid and ethanol production were inversely related, with ethanol production promoted at 10°C and 25°C and acetic acid production promoted at AT and 40°C. The bacterial diversity was reduced in TMR silage with high lactic acid and acetic acid content, and the fungal diversity was reduced in TMR silage with high ethanol content. Conclusions: The intensity of lactic acid production was accounted for by the high abundance of Lactobacillus, and its stagnated production at a substantially high storage temperature was related to an increased abundance of Bacillus. The enhanced production of acetic acid or ethanol can be explained by differences in the fungal microbiota. Significance and Impact of the Study: The integrated analysis of bacterial and fungal microbiota can provide in‐depth insights into the impact of storage temperature on TMR silage fermentation. [ABSTRACT FROM AUTHOR]

This case report investigated the outbreak of aflatoxicosis in a dairy herd in Pakistan, which resulted in 30 abortions of 40 confirmed (75%) pregnant cows in a period of 35 days and in 18.8% depression of farm average milk production for the entire herd. The analysis of the concentrate feed of the total mixed ration (TMR), using enzyme-linked immunosorbent assay (ELISA) procedures from two different local laboratories, indicated concentrations of 60 μg/kg dry matter (DM) of aflatoxin B1 (AFB1) and 100 μg/kg DM of total aflatoxins (AFs: sum of B1, B2, G1 and G2). Subsequently, a confirmatory analysis with a more sensitive and validated multi-metabolite liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was performed. This analysis detected a concentration of total AFs in the TMR of 166 μg/kg DM ± 3.5 (AFB1:134, AFB2:17.4 and AFM1:14.9 μg/kg DM). The concentrate feed (55% of the TMR DM) was confirmed as a source of contamination, presenting a concentration >29 times higher than the EU-maximum limit value (5.68 μg/kg DM). Additionally, the multi-mycotoxin analysis evidenced the co-occurrence of 81 other toxic and potentially toxic fungal metabolites in the fed TMR. After replacing the contaminated concentrate feed with feedstuffs of the same formulation but from a new charge of ingredients, the abortion episodes ceased, and milk production increased significantly. In conclusion, the data of this case report suggest that AFs may be associated with pregnancy losses in dairy cattle and milk production depression. From the public health perspective, the data also indicate the need for a more careful examination of dairy animal feed in Pakistan. Since the high concentration of AFB1 detected in feed and considering the literature-reported transfer rates (1–6%) of this toxin to AFM1 (carcinogen for humans) in milk, the milk produced during the outbreak period is expected to be contaminated with AFM1, which raises public health concerns. [Display omitted] • Aflatoxicosis outbreak in a Pakistani dairy herd led to a 75% abortion rate and an 18.8% decrease in milk yield. • The concentrate feed was identified as the primary source of AFB1, exceeding EU limits by 29 times. • Multitarget LC-MS/MS was employed to accurately quantify mycotoxins' concentrations in the feed. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to determine the appropriate supplementation level of lactic acid bacteria (LAB; Lactobacillus plantarum and Bacillus clausii), yeast (Saccharomyces cariocanus and Wickerhamomyces anomalus) for degrading free gossypol and glucosinolate in the fermented total mixed ration (TMR) containing cottonseed meal (CSM) or rapeseed meal (RSM), to improve the utilization efficiency of these protein sources. Methods: For LAB, L. plantarum or B. clausii was inoculated at 1.0×108, 1.0×109, 1.0×1010, and 1.0×1011 colony-forming unit (CFU)/kg dry matter (DM), respectively. For yeast, S. cariocanus or W. anomalus was inoculated at 5×106, 5×107, 5×108, and 5×109 CFU/kg DM, respectively. The TMR had 50% moisture and was incubated at 30°C for 48 h. After fermentation, the chemical compositions, and the contents of free gossypol and glucosinolate were determined. Results: The results showed that the concentration of free gossypol content was reduced (p<0.05), while that of the crude protein content was increased (p<0.05) in the TMR containing CSM inoculated by B. clausii (1×109 CFU/kg DM) or S. cariocanus (5×109 CFU/kg DM). Similarly, the content of glucosinolate was lowered (p<0.05) and the crude protein content was increased (p<0.05) in TMR containing RSM inoculated with B. clausii (1×1010 CFU/kg DM) or S. cariocanus (5×109 CFU/g DM). Conclusion: This study confirmed that inclusion of B. clausii with 1.0×109 or 1.0×1010 CFU/kg DM, or S. cariocanus (5×109 CFU/kg DM) to TMR containing CSM/RSM improved the nutritional value and decreased the contents of anti-nutritional factors. [ABSTRACT FROM AUTHOR]

The study was to screen the best probiotic combination suitable for the production of fermented total mixed rations for mutton sheep. In the study, the effect of eight strain combinations (Saccharomyces cerevisiae + Bacillus subtilis + Lactobacillus delbrueckii subsp. lactis + Aspergillus niger + Aspergillus oryzae, Candida utilis + Bacillus subtilis + Lactobacillus delbrueckii subsp. lactis + Aspergillus niger + Aspergillus oryzae, Saccharomyces cerevisiae + Bacillus subtilis Bacillus + Bifidobacterium bifidum + Aspergillus niger + Aspergillus oryzae, Candida utilis + Bacillus subtilis + Bifidobacterium bifidum + Aspergillus niger + Aspergillus oryzae, Saccharomyces cerevisiae + Candida utilis + Bacillus subtilis + Lactobacillus delbrueckii subsp. lactis + Aspergillus niger + Aspergillus oryzae, Saccharomyces cerevisiae + Candida utilis + Bacillus subtilis + Bifidobacterium bifidum + Aspergillus niger + Aspergillus oryzae, Saccharomyces cerevisiae + Aspergillus niger + Aspergillus oryzae, Saccharomyces cerevisiae) with seven beneficial bacteria (Saccharomyces cerevisiae, Candida utilis, Bifidobacterium bifidum, Bacillus subtilis, Lactobacillus delbrueckii subsp. lactis, Aspergillus niger and Aspergillus oryzae) on nutritional value and mycotoxins of fermented total mixed diet for mutton sheep were studied by conventional nutrient composition analysis and ELISA methods. In the study, the addition amount of Saccharomyces cerevisiae and Candida utilis in feed was 0.86%, and the addition amount of other strains was 0.43%. The control group was TMR directly mixed without any beneficial bacteria. The fermentation time was 8 d. The results showed that compared with control group, the combination of multiple strains could significantly increase the true protein content in FTMR and decrease the neutral detergent fiber content (P<0.05), and group E had the best effect. Compared with control group, multiple strains combination could significantly reduce the content of aflatoxin and emitoxin in FTMR (P<0.05), the most significant decrease of aflatoxin content was in group E (P<0.05), and the most significant decrease of emitoxin content was in groups H and G (P<0.05). The experiment indicates that the combination of different strains can effectively improve the feed quality of FTMR and reduce the content of mycotoxins. The best effect is the E group. [ABSTRACT FROM AUTHOR]

An experiment was conducted to study the effect of supplementation of thermostable yeast in total mixed ration on rumen fermentation parameters by in vitro gas production technique in crossbred cows. Saccharomyces cerevisiae was supplemented in the total mixed ration at various doses viz., 0 (control), 0.25 × 106, 0.5 × 106, 1 × 106, 2 × 106 and 4 × 106 CFU. The in vitro true dry matter degradability, in vitro true organic matter degradability, total gas production, metabolisable energy, volatile fatty acid production and microbial biomass production increased proportionally to the dose of S. cerevisiae. Methane production was significantly lowered by supplementation. Results revealed that the S. cerevisiae supplementation can modify ruminal fermentation and were dose dependent. [ABSTRACT FROM AUTHOR]

One of the major issues confronting the dairy industry is the efficient evaluation of the quality of feed, milk and dairy products. Over the years, the use of rapid analytical methods in the dairy industry has become imperative. This is because of the documented evidence of adulteration, microbial contamination and the influence of feed on the quality of milk and dairy products. Because of the delays involved in the use of wet chemistry methods during the evaluation of these products, rapid analytical techniques such as near-infrared spectroscopy (NIRS) has gained prominence and proven to be an efficient tool, providing instant results. The technique is rapid, nondestructive, precise and cost-effective, compared with other laboratory techniques. Handheld NIRS devices are easily used on the farm to perform quality control measures on an incoming feed from suppliers, during feed preparation, milking and processing of cheese, butter and yoghurt. This ensures that quality feed, milk and other dairy products are obtained. This review considers research articles published in reputable journals which explored the possible application of NIRS in the dairy industry. Emphasis was on what quality parameters were easily measured with NIRS, and the limitations in some instances. [ABSTRACT FROM AUTHOR]

The objective of the work is to evaluate the effects of four chemical additives on fermentation quality, aerobic stability, and in vitro ruminal digestibility of total mixed ration (TMR) silage. TMR containing 15% spent mushroom substrate, 25% soybean sauce residue, 45% napiergrass (Pennisetum purpureum (L.) Schum.), and 15% concentrate was ensiled with the following: (1) no additives (control), (2) potassium sorbate (PS, 0.1%), (3) sodium benzoate (SB, 0.1%), (4) sodium diacetate (SDA, 0.5%), and (5) calcium propionate (CAP, 0.5%) based on fresh weight. All silos (10 L) were opened for fermentation quality, in vitro ruminal digestibility analysis after 60 days of ensiling, and then subjected to aerobic stability test for 9 days. All TMR silages were well-conserved, as indicated by low pH, butyric acid, and ammonia nitrogen contents. During aerobic exposure, SDA was more stable with higher (p < 0.05) lactic acid and acetic acid contents and lower (p < 0.05) yeast counts than other TMR silages. In addition, SDA significantly (p < 0.05) increased cumulate gas production and in vitro dry matter digestibility compared with the control. Overall, SDA is recommended as additives to improve fermentation quality, in vitro ruminal digestibility, and aerobic stability of TMR silage prepared with local food by-products. [ABSTRACT FROM AUTHOR]

The aim of this study is to investigate the effects of total mixed ration (Control, C), fermented total mixed ration (Control Fermented, CF) and fermented fattening feed prepared with by-products (By-product Fermented, BF) on fattening performance in Brangus male bulls. In the research, a total of 114 Brangus fattening bulls were used and divided into 3 main groups and 5 subgroups were formed under each main group. All bulls were weighed at the beginning of fattening and then monthly, dry matter consumption, daily live weight gains and feed conversion ratios were calculated. There was no statistical difference between the groups in the initial and final body weights of the animals (P>0.05). The average daily gain of the bulls were determined in the band of approximately 1.50 kg and no statistical difference was found between the groups (P>0.05). On the other hand, dry matter intake was determined as 10.68, 11.66 and 10.64 kg/day in the C, CF and BF groups, respectively, and a trend was found between the groups (P=0.08). Likewise, feed conversion ratios were determined as 6.93, 7.52 and 7.09 in the CK, CF and BF groups, respectively, and a trend was found between the groups (P=0.08). On the other hand, bulls fed with BF group; The group fed with K can reach the same body weight on the same days and are fed 2.81 TL cheaper per animal per day. It can be concluded that if by-products are fermented and given to Brangus beef cattle, there is no difference in fattening performance compared to other groups, but it provides great economic benefits. [ABSTRACT FROM AUTHOR]

Introduction: This review addresses metabolic diversities after grain feeding of cattle using artificial total mixed ration (TMR), in place of pasture-based feeding. Objectives: To determine how grain feeding impairs the deuterium-depleting functions of the anaplerotic mitochondrial matrix during milk and meat production. Methods: Based on published data we herein evaluate how grain-fed animals essentially follow a branched-chain amino acid and odd-chain fatty acid-based reductive carboxylation-dependent feedstock, which is also one of the mitochondrial deuterium-accumulating dysfunctions in human cancer. Results: It is now evident that food-based intracellular deuterium exchange reactions, especially that of glycogenic substrate oxidation, are significant sources of deuterium-enriched (2H; D) metabolic water with a significant impact on animal and human health. The burning of high deuterium nutritional dairy products into metabolic water upon oxidation in the human body may contribute to similar metabolic conditions and diseases as described in state-of-the-art articles for cows. Grain feeding also limits oxygen delivery to mitochondria for efficient deuterium-depleted metabolic water production by glyphosate herbicide exposure used in genetically modified crops of TMR constituents. Conclusion: Developments in medical metabolomics, biochemistry and deutenomics, which is the science of biological deuterium fractionation and discrimination warrant urgent critical reviews in order to control the epidemiological scale of population diseases such as diabetes, obesity and cancer by a thorough understanding of how the compromised metabolic health of grain-fed dairy cows impacts human consumers. [ABSTRACT FROM AUTHOR]

Sixty Hanwoo steers of average weight and average age 6.5 months were raised until the age of 18 months (growing period, G) and from the age of 19 months to 30.85-31.52 months (fattening period, F). They were separately fed concentrate and forage (C) or total mixed ration (TMR, T) in the growing and fattening periods. The treatments were GCFC (Concentrate feeding in the growing and fattening periods), GCFT (Concentrate feeding in the growing period and TMR feeding in the fattening period), GTFC (TMR feeding in the growing period and concentrate feeding in the fattening period) and GTFT (TMR feeding in the growing and fattening periods) in a completely randomized design. Dry matter intake (p < 0.001) in the growing period was higher in the GCFC and GCFT groups than in the other groups, whereas DMI of TMR feeding (GCFT and GTFT) during fattening period increased by 3.3% (p < 0.001). The longissimus dorsi muscle area (LMA, 94.20 vs. 96.73 cm²) and back fat thickness (BFT 17.33 vs. 16.93 mm) were not significantly affected during fattening period among different treatments. Marbling score, which substantially affects meat quality, the GCFT and GTFT groups in the fattening period presented higher than the GTFC and GCFC groups. The incidence of C grade of meat yield was 33.3% with GCFC and GTFC) and 40.0% with GCFT and GTFT). The incidence of grade 1+ or higher meat quality was higher with (GCFT, 86.6%; GTFT, 73.3%) than (GCFC, 46.7%; GTFC, 66.6%) in the fattening period. In conclusion, feeding TMR to beef cattle has been shown to have a positive effect on productivity. Therefore, it is expected that TMR can help to improve the competitiveness of beef cattle farms. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the effects of feeding untreated or urea-treated ground corncob and supplementing with cassava leaf meal (CLM) in a total mixed ration on growth, feed intake, nutrient digestibility, nitrogen (N) utilization and rumen fermentation of sheep. Five diet treatments with five replications consisted of untreated corncob + concentrate (CC); urea-treated corncob + concentrate (UCC); and CC and UCC supplemented with CLM and designated as CC+CLM and UCC+CLM, respectively. A diet formulated with a mixture of elephant grass and concentrate was used as control (EG). There were no significant differences in dry matter intake (DMI) between treatments. The average DMI was 4.17% bodyweight. Average daily gain (ADG) of sheep fed the treatment diets was between 146.3 and 176.2 g/h/day, and was higher than EG (89.1 g/head/day). Thus, the treatments improved feed conversion ratio (FCR). Nitrogen retention was lowest for EG and highest for UCC and UCC+CLM. The addition of CLM had no effect on growth, feed intake, nutrient digestibility, nitrogen utilization and rumen fermentation characteristics. It was concluded that corncob could be used as a replacement for elephant grass in postweaning diets for sheep. [ABSTRACT FROM AUTHOR]

Cobalt lactate is a highly soluble ruminal Co source. Prior research evaluating higher Co feeding rates demonstrated increased ruminal fiber digestion. Feeding high-forage (>70%) rations to late-lactation dairy cows to enhance income over feed cost could potentially benefit from higher ruminal-soluble Co inclusion rates to enhance ruminal fiber and nutrient digestibility. Twenty-four late-lactation [238 ± 68.8 d in milk (DIM) and 36.4 ± 5.4 kg/d milk] Holstein dairy cows (10 primiparous and 14 multiparous) were blocked by milk yield, DIM, and parity, and randomly assigned to 1 of 2 treatments. Treatments were (1) control, CoCO 3 total mixed ration (TMR) containing 12.5 mg/cow per day of cobalt (cobalt carbonate), and (2) cobalt lactate (Co-LAC), same CoCO 3 TMR but containing an additional 50 mg/cow per day of Co, via a 1% cobalt lactate product (CoMax, Ralco Inc.). The basal TMR was 70% forage [60% alfalfa baleage and 40% corn silage on a dry matter (DM) basis] and 30% of the respective experimental grain mix on a DM basis. Cows were fed the CoCO 3 TMR during the 7-d covariate period, followed by 4 wk of data collection when CoCO 3 and Co-LAC TMR were fed. Milk production (26.2 and 25.8 kg/d for CoCO 3 and Co-LAC, respectively) and dry matter intake (DMI; 22.9 and 23.1 kg/d) were similar for cows fed both Co treatments. Production of milk fat (1.02 and 1.09 kg/d), milk protein (0.87 and 0.91 kg/d), and lactose (1.17 and 1.26 kg/d) were similar for cows fed both treatments. Body weights (684 and 674 kg) were similar for cows fed both treatments. Rumen ammonia concentrations (15.8 and 12.3 mg/dL) were lower for cows fed Co-LAC compared with cows fed CoCO 3 (15.8 mg/dL). Ruminal molar acetate concentrations (59.5 and 61.1%) were greater for cows fed Co-LAC compared with cows fed CoCO 3. Feeding additional Co as cobalt lactate in the TMR did not enhance lactational performance (milk production, composition, DMI, or body weight changes) when fed a high-forage TMR but altered ruminal fermentation with decreased ruminal ammonia and increased ruminal acetate concentrations. [ABSTRACT FROM AUTHOR]

Correlating volatile compounds with the sensory attributes of whole milk powder (WMP) is fundamental for appreciating the effect of lipid oxidation (LO) on sensory perception. LO compounds can adversely affect the sensory perception of WMP by imparting rancid, metallic, and painty notes. Whole milk powders derived from milk produced by cows maintained on a pasture diet (grass and grass-clover mix) versus a nonpasture diet [total mixed ration (TMR); concentrates and silage] were stored at room temperature 21°C (ambient storage) and 37°C (accelerated storage) and analyzed for volatile compounds and sensory attributes every 2 mo for a total of 6 mo. Thirteen volatile compounds originating from LO were chosen to track the volatile profile of the WMP during storage. Color, composition, total fatty acid, and free fatty acid profiling were also carried out. Significant variations in the concentrations of 14 fatty acids were observed in WMP based on diet. Concentrations of free fatty acids increased in all sample types during storage. Similar trends in sensory attributes were observed with an increase in painty attributes, corresponding to an increase in hexanal. Buttery/toffee attributes were found to be more closely correlated with TMR WMP. Those WMP derived from pasture diets were found to be more susceptible to LO from a volatile perspective, particularly in relation to aldehyde development, which is likely due to increased concentrations of conjugated linoleic acid and α-linolenic acid found in these samples. [ABSTRACT FROM AUTHOR]

Objective: Our recent findings confirmed the effectiveness of sodium metabisulfite (SMB) in controlling the growth of undesirable microorganisms in fruit and vegetable discards (FVD); however, lactic acid bacteria (LAB) are susceptible to its antibacterial effects. Two series of experiments were conducted to enable the survivability of LAB during silage fermentation of a total mixed ration (TMR) containing SMB-treated FVD. Methods: In Exp. 1, the objective was to isolate a strain of LAB tolerable to the toxic effect of SMB. In Exp. 2, the SMB load was minimized through its partial replacement with a chemical mixture (CM) based on sodium benzoate (57%), potassium sorbate (29%), and sodium nitrite (14%). FVD was treated with SMB + CM (2 g each/kg biomass) and added to the TMR at varying levels (0%, 10%, or 20%), with or without KU18 inoculation. Results: The KU18 was screened as a presumptive LAB strain showing superior tolerance to SMB in broth medium, and was identified at the molecular level using 16S rRNA gene sequence analysis as Lactobacillus plantarum. Inoculation of KU18 in TMR containing SMB was not successful for the LAB development, biomass acidification, and organoleptic properties of the resultant silage. In Exp. 2, based on the effectiveness and economic considerations, an equal proportion of SMB and CM (2 g each/kg FVD) was selected as the optimal loads for the subsequent silage fermentation experiment. Slight differences were determined in LAB development, biomass acidification, and sensorial characteristics among the experimental silages, suggesting the low toxicity of the preservatives on LAB growth. Conclusion: Although KU18 strain was not able to efficiently develop in silage mass containing SMB-treated FVD, the partial substitution of SMB load with the CM effectively alleviated the toxic effect of SMB and allowed LAB development during the fermentation of SMB + CM-treated FVD in TMR. [ABSTRACT FROM AUTHOR]

The present study was undertaken to evaluate the effect of isonitrogenous diets varying in concentrate-to-forage ratio on enteric methane (CH4) emission, nutrient utilization and growth performance in crossbred goat kids. Fifteen Alpine and Beetal crossbred (AxB) kids (3–4 months age) were divided into three groups based on average body weight and treatments were assigned to the three groups randomly. The first group (GP-I) received a total mixed ration (TMR) containing concentrate mixture and berseem fodder in the ratio of 70:30. The TMR of the second (GP-II) and third (GP-III) groups consisted of concentrate mixture and berseem fodder in the ratio of 50:50 and 20:80, respectively. The results of 90 days feeding experiment revealed that the intake of dry matter (DM), crude protein (CP) and total digestible nutrients (TDN) was significantly higher (p < 0.05) in GP-I than GP-II and GP-III. Digestibility coefficients of DM and CP were unaffected by the treatments, however neutral detergent fibre (NDF) and acid detergent fibre (ADF) digestibility increased significantly in GP-II and GP-III compared to GP-I. The emission of enteric CH4 reduced (p < 0.05) by 34.18% in GP-I in contrast to GP-III. Average daily gain (ADG) was significantly higher (p < 0.05) in GP-I (115.99 g/day) compared to GP-II (80.94 g/day) and GP-III (77.42 g/day), however the feed efficiency remained unaltered. It was concluded that increased level of concentrate in TMR reduced methane emission and impacted the growth performance positively in crossbred goat kids. [ABSTRACT FROM AUTHOR]

The milk production of high producing European dairy cattle breeds in tropical environment is not in satisfaction level. In general, cows develop negative energy balance at the early lactation resulting poor production performances. The aim of the study was to assess the effect of total mixed ration feeding systems (TMR) over conventional feeding system to increase the production performances of cows in early lactation. Napier (Pennisetum purpureum) based conventional feeding system (CFS) versus two different types of total mixed rations as TMR1 (with the same ingredients and same quantity in CFS) and TMR2 (16% dry matter of napier in TMR1 was replaced with legume and non-legume) were studied on production performances of 15 Holstein Friesian cows in early lactation (n=5). The highest average daily milk yield was observed in TMR2 fed cows (17.88±3.03 L/d) while the lowest was observed in CFS fed cows (12.23±0.88 L/d, P<0.0001). The percentage of average daily weight gain was differed (P=0.01) among the treatments (CFS=1.53%, TMR1=2.79%, and TMR2=4.32%). The body condition score of the cows fed with TMR1 and TMR2 was increased by 0.1 and 0.25, respectively at the end of the experimental period whereas it was unchanged in the cows fed with CFS. Results revealed that feeding of TMR for Holstein Friesian cows in early lactation is a better way to achieve increased milk production with a better body condition in the tropics. TMR consisted of legume and non-legume fodder was more efficient. [ABSTRACT FROM AUTHOR]

Objective: This study aimed to determine the effects of different roughages in total mixed ration (TMR) inoculated with or without coculture of Lactobacillus acidophilus (L. acidophilus) and Bacillus subtilis (B. subtilis) on in vitro rumen fermentation and microbial population. Methods: Three TMRs formulations composed of different forages were used and each TMR was grouped into two treatments: non-fermented TMR and fermented TMR (F-TMR) (inoculated with coculture of L. acidophilus and B. subtilis). After fermentation, the fermentation, chemical and microbial profile of the TMRs were determined. The treatments were used for in vitro rumen fermentation to determine total gas production, pH, ammonia- nitrogen (NH3-N), and volatile fatty acids (VFA). Microbial populations were determined by quantitative real-time polymerase chain reaction (PCR). All data were analyzed as a 3x2 factorial arrangement design using the MIXED procedure of Statistical Analysis Systems. Results: Changes in the fermentation (pH, lactate, acetate, propionate, and NH3-N) and chemical composition (moisture, crude protein, crude fiber, and ash) were observed. For in vitro rumen fermentation, lower rumen pH, higher acetate, propionate, and total VFA content were observed in the F-TMR group after 24 h incubation (p<0.05). F-TMR group had higher acetate concentration compared with the non-fermented group. Total VFA was highest (p<0.05) in F-TMR containing combined forage of domestic and imported source (F-CF) and F-TMR containing Italian ryegrass silage and corn silage (F-IRS-CS) than that of TMR diet containing oat, timothy, and alfalfa hay. The microbial population was not affected by the different TMR diets. Conclusion: The use of Italian ryegrass silage and corn silage, as well as the inoculation of coculture of L. acidophilus and B. subtilis, in the TMR caused changes in the pH, lactate and acetate concentrations, and chemical composition of experimental diets. In addition, F-TMR composed with Italian ryegrass silage and corn silage altered ruminal pH and VFA concentrations during in vitro rumen fermentation experiment. [ABSTRACT FROM AUTHOR]

Palm kernel meal (PKM) is a byproduct produced after oil extraction from palm kernels. It is a valuable source of fiber, energy and protein which can reduce the cost of total mixed ration (TMR). This study was conducted to assess the growth performance, carcass characteristics and meat quality of lambs fed TMR pellet with PKM. A total of 32, three-month-old male lambs were randomly distributed into two diets (PTMR1= 0; PTMR2= 200 kg PKM/ton TMR). Results of the study showed that the production performance and the characteristics, composition and fat content of carcasses, as well as meat quality traits were not affected by PTMR2. The relative weight of the loin and tail and cohesiveness were lower while the index of myofibril fragmentation was higher when compared to PTMR1. The pH and color of the carcass and meat components were not significantly different except for hindquarter color (Lightness) and the ultimate meat pH in PTMR2. The pH value and tissue color (Redness) of rumen were higher when the lambs were fed with PTMR2 compared to PTMR1. The study indicates that 20% PKM addition to TMR may be a safe strategy for lamb feeding with no negative impact on growth performance, carcass characteristics and meat quality of lambs. [ABSTRACT FROM AUTHOR]

The aim of this study was to develop near-infrared spectroscopy (NIRS) prediction models for the estimation of chemical components and the fibre undegradable fractions (uNDF) of hay-based total mixed rations (TMR). A total of 205 TMR samples were used for the study. All the chemical components were measured using standard AOAC reference methods and expressed as percentages of dry matter (DM). Prediction models were developed using both cross- and independent validation and different mathematical treatments applied on spectral data. The best spectral treatment was chosen based on the method which simultaneously achieved the lowest root mean square error and the highest explained variance in cross-validation. The coefficient of determination in external validation (R2P) was the greatest for starch prediction model (R2P = 0.84), followed by acid detergent fibre (ADF; R2P = 0.79), and amylase-treated ash-corrected NDF with addition of sodium sulphite (aNDFom) and crude protein prediction models (CP; R2P = 0.73). The concordance correlation coefficient (CCC) in validation ranged from 0.66 (ash prediction model) to 0.92 (starch prediction model), indicating substantial to accurate models' predictive ability. This study indicated that NIRS can be a screening method for the prediction of CP, Starch, aNDFom, ADF, acid detergent lignin (ADL), uNDF and Ash. The use of TMR utilised in various herds provided high variability for the NIRS calibration dataset, implying that the developed NIRS pre-diction models could be applicable to TMR collected from herds located in the Parmigiano Reggiano cheese production area. NIRS can be successfully employed to determine quickly and at cost-effective different compositional and digestibility traits in hay-based TMR. TMR analysis predicted by NIRS can support nutritionists in the formulation of diets containing a proper nutrient profile to sustain physiological, metabolic, and immunological processes. The use of NIR technology for TMR analysis can allow frequent monitoring of rations and increasingly timely corrections, maximising cows' diet utilisation and conversion of the ingested feed. [ABSTRACT FROM AUTHOR]

This study was aimed to analysis the effects of roughage sources and oil sources in a total mixed ration (TMR) on voluntary feed intake, digestibility and blood chemistry. The study was conducted using four crossbred Thai Native X American Brahman cattle, aged 1.5 years old and with a live weight of 177±34 kg, which were trialled by using a 2x2 factorial in 4×4 Latin square design. All cattle received TMR diets supplemented with bagasse and palm oil, bagasse and sunflower oil, rice straw and palm oil, rice straw and sunflower oil. Each period of feeding lasted for 21 days. During the experimental periods, all cattle were fed ad libitum and with a water supply. The results revealed that roughage sources and oil sources did not affect voluntary feed intake, digestibility and blood chemistry. It was concluded from this experiment that using bagasse or rice straw as the roughage sources with palm oil and sunflower oil mixed in the TMR were suitable for use in beef cattle diets, especially using bagasse with palm oil because of the lowest price (0.28 USD/kg) when compared to the other treatments. [ABSTRACT FROM AUTHOR]

The objective was to determine the effects of converting calves from a component-fed ration to a total mixed ration (TMR) at 8, 10, or 12 wk of age on intake, growth, and nutrient digestibility. Holstein calves (n = 40) were randomly assigned to 1 of 4 groups (no TMR, TMR conversion at 8, 10 or 12 wk; T0, T8, T10, and T12, respectively). Calves were weaned at 6 wk of age, housed individually, and studied from 7 to 14 wk of age. Rations, consisting of a 20% crude protein texturized starter and grass hay, were offered ad libitum as separate components or as a TMR with 85% starter and 15% grass hay on a dry matter (DM) basis. Intakes and body weights (BW) were measured weekly. Component intake for TMR was calculated from the proportion of grass hay and starter contained in the TMR. Fecal grab samples were collected every 9 h over 3 d for a total of 8 samples that formed a composite at 9, 11, and 13 wk of age from the same 4 calves per group. Rumen fluid samples were collected via esophageal tube at −1, 0, 3, and 7 d relative to conversion from component to TMR. Feed and feces were evaluated for DM, neutral detergent fiber, acid detergent fiber, and acid detergent lignin (internal flow marker) to estimate digestibility. Average daily gain and final BW tended to be least for T8. Empty BW gain was significantly less for T8 than for T0 but not different from T10 or T12 (T0 = 1.07, T8 = 0.93, T10 = 1.02, T12 = 1.04 kg/d). Hip growth tended to be least for T8 and resulted in lower final hip width (T0 = 25.9, T8 = 25.2, T10 = 25.6, T12 = 25.8 cm). Intake tended to be reduced for calves converted to TMR earlier. Throughout the study, calves fed TMR ate more hay and less starter than component-fed calves. Metabolizable energy intake was less for T8 versus T0 but not different from T10 or T12 (T0 = 8.46, T8 = 7.55, T10 = 8.01, T12 = 8.23 Mcal/d). We observed no differences in feed efficiency for the duration of the study. Differences in DM digestibility were not observed, but fiber digestibility was greater for calves fed TMR at 9, 11, and 13 wk of age. Conversion to TMR increased rumen pH. These results indicate that TMR conversion increased hay consumption and subsequently decreased starter and total DM intake. This led to reduced weight and structural growth; however, calves that were converted to TMR as early as 8 wk still achieved adequate growth. The increase in rumen pH and subsequent increase in fiber digestibility allowed for calves to be converted to a 15% grass hay TMR as early as 8 wk and still achieve desirable growth goals. [ABSTRACT FROM AUTHOR]

To improve the utilization efficiency of total mixed ration (TMR) on Tibetan plateau, the effects of different additives on fermentation quality and aerobic stability of the ensiled TMR prepared with local feed resources were studied. A total of 150 experimental silos were prepared in a completely randomized design to evaluate the following treatments: (a) control; (b) Lactobacillus buchneri; (c) acetic acid; (d) propionic acid; (e) 1,2-propanediol; and (f) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and in vitro parameters analysis, and then subjected to an aerobic stability test for 14 days. The acetic acid, 1,2-propanediol and 1-propanol treatments increased (p < .05) pH and acetic acid content, and lowered (p < .05) the lactic acid production in comparison to control. There were no statistically significant differences in in vitro digestibility parameters among the treatments. Treatments of acetic acid, 1,2-propanediol and 1-propanol substantially improved the aerobic stability of the ensiled TMR, as indicated by almost unchanged pH and lactic acid contents throughout the aerobic exposure test. These results indicated that acetic acid, 1,2-propanediol and 1-propanol had no adverse effect on in vitro digestibility and could be effective additives for enhancing the aerobic stability of ensiled TMR prepared on Tibetan plateau. [ABSTRACT FROM AUTHOR]

To effectively utilize total mixed ration (TMR) prepared with locally available feed resources, we studied the nutritional value and milk production in Jersey dairy cattle fed a local general diet (LGD) and fermented TMR in Mozambique. Ten head of Jersey dairy cattle with 337 ± 19.8 kg body weight, aged 3-4 years in mid location were used in this study. The LGD diet was designed following the general feeding method of local smallholding farms; it contained native grass, Napier grass, wheat bran, and mineral-vitamin mix. Fermented TMR was prepared using Napier grass, corn bran, wheat bran, formula feed, and mineral-vitamin mix. Fermented TMR was preserved as good quality, with a relatively low pH and high lactic acid content. Compared to LGD, fermented TMR significantly improved the dry matter intake and digestibility in dairy cattle. Milk yield was significantly higher in dairy cattle fed with TMR than in those fed with LGD, by 3.75 L/d; milk quality was not significantly different between treatments. The results confirmed that LGD had a low dry matter intake and milk yield, and fermented TMR prepared with local feed resources can attain good quality and improve milk yield in dairy cattle in Mozambique. [ABSTRACT FROM AUTHOR]

This study examined the effect of dietary factors on compositional and functional properties of whole milk powder (WMP) produced from bovine milk. Raw milk samples were obtained from 3 groups of 18 Holstein Friesian spring-calving cows randomly assigned to diets based on perennial ryegrass (GRS), perennial ryegrass/white clover sward (CLV), and total mixed ration (TMR). Raw milks obtained in late lactation were subsequently standardized for fat, heat-treated (90°C for 30 s), evaporated, and homogenized before spray drying. The WMP produced from each diet were analyzed to determine differences in color, particle size distribution, heat coagulation time, yogurt gelation, texture profile, and protein profile due to each diet. Significant differences in heat coagulation time were observed between the CLV and TMR samples, whereas color values were significantly different between GRS and TMR samples. No significant differences in gross composition, protein profile, or whey protein nitrogen index were found between the 3 WMP samples. Average D 90 values (the particle size at which 90% of the particles were smaller than the specified size) for fat globules were significantly lower in the TMR sample compared with the GRS and CLV samples. Yogurts produced from GRS- and CLV-derived WMP had significantly higher elastic moduli (G′) than those produced from TMR-derived WMP. Similarly, texture profile analysis revealed significantly higher firmness values in yogurt samples derived from CLV compared with TMR samples. Our data characterize the effect of these diets on the composition and functional properties of fat-standardized WMP, suggesting better yogurt functionality and thermal stability in WMP derived from pasture-based bovine diets. [ABSTRACT FROM AUTHOR]

Objectives of the experiment were to determine effects of dietary hay concentration in a dry total mixed ration and its carryover effects on intake, growth performance, faecal score, and feed efficiency of weaned dairy calves. Eighteen Friesian x Jersey weaned calves (n = 6 calves/treatment) were randomly assigned to three rhodes grass hay treatments (RG13, RG26, and RG39). The experimental diets were rhodes grass hay-based total mixed rations containing 13%, 26%, and 39% chopped hay on a DM basis. The experiment had two phases of four weeks each. In phase 1 (weeks 1-4), weaned calves were fed RG13, RG26, or RG39. Then, in phase 2 (weeks 5-8), all calves were shifted to a maize silage-based diet. All the diets were iso-nitrogenous and were fed ad libitum. Calves were housed in individual pens and had free access to water and feed. Average daily gain and daily dry matter intake were analysed as repeated measures, whereas bodyweight and feed efficiency were analysed using one-way ANOVA. In phases 1 and 2 dry matter intakes were similar. Growth rate decreased linearly with increasing concentration of hay in phase 1. Overall, daily dry matter intake, average daily gain, change in body condition score and structural measurements were not affected by dietary treatments. However, overall feed efficiency was improved for calves fed RG26 compared with RG13 and RG39. Thus, feeding a moderate level of hay had positive impacts on the transition to a silage-based TMR. [ABSTRACT FROM AUTHOR]

Low-heat skim milk powder (LHSMP) was manufactured on 3 separate occasions in mid lactation (ML, July 4–20) and late lactation (LL, September 27 to October 7) from bulk milk of 3 spring-calving dairy herds on different feeding systems: grazing on perennial ryegrass (Lolium perenne L.) pasture (GRO), grazing on perennial ryegrass and white clover (Trifolium repens L.) pasture (GRC), and housed indoors and offered total mixed ration (TMR). The resultant powders (GRO-SMP, GRC-SMP, and TMR-SMP) were evaluated for composition and color and for the compositional, physicochemical, and processing characteristics of the reconstituted skim milk (RSM) prepared by dispersing the powders to 10% (wt/wt) in water. Feeding system significantly affected the contents of protein and lactose, the elemental composition, and the color of the LHSMP, as well as the rennet gelation properties of the RSM. The GRO and GRC powders had a higher protein content; lower levels of lactose, iodine, and selenium; and a more yellow-green color (lower a* and higher b* color coordinates) than TMR powder. On reconstitution, the GRO-RSM had higher concentrations of protein, casein, and ionic calcium, and lower concentrations of lactose and nonprotein nitrogen (% of total N). It also produced rennet gels with a higher storage modulus (G′) than the corresponding TMR-RSM. These effects were observed over the combined ML and LL period but varied somewhat during the separate ML and LL periods. Otherwise, feeding system had little or no effect on proportions of individual caseins, concentration of serum casein, casein micelle size, casein hydration, heat coagulation time, or ethanol stability of the RSM at pH 6.2 to 7.2, or on the water-holding capacity, viscosity, and flow behavior of stirred yogurt prepared by starter-induced acidification of RSM. The differences in the functionality of the LHSMP may be of greater or lesser importance depending on the application and the conditions applied during the processing of the RSM. [ABSTRACT FROM AUTHOR]

The purpose of this study is to examine the effects of body condition score (BCS) on nutritional diseases and milk yield in dairy cattle. Fifty-nine randomly selected Holstein Friesian cattle, lactation numbers 3-6, were used in this study from a total of 350 dairy cattle in the establishment. All of the cows in the study were fed with the same total mixed ration (TMR), and BSC was determined by inspection and palpation in the first 5 days after calving. The cows were separated into two groups according to their BCS score as BCS ≤ 3 and BCS ≤ 3, and Group 1 contained 36 cows (BCS ≤ 3), while Group 2 contained 23 cows (BCS ≤ 3). One-month, 3-month, and 305-day milk yields and nutritional diseases as observed until postpartum day 35 have been determined in this study. Moreover, TMR analysis has been performed. As a result, the 1-month, 3-month, and 305-day milk yields were determined in Group 1 (BCS ≤ 3) and Group 2 (BCS ≤ 3), and no difference was determined between the groups (P > 0.05). [ABSTRACT FROM AUTHOR]