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Background: Microcirculation is the flow of blood through the smallest vessels in the circulatory system. Capillaries respond to various pathologies much earlier than arteries and veins, the changes which indicate that the disease is already developing. Careful monitoring of the functioning of the capillary system often allows the detection of in vivo disorders at an early stage. Aims: The aim of the study was to evaluate the microcirculation within acne lesions. A noninvasive method of semi‐quantitative blood perfusion measurement based on laser speckle contrast analysis (LASCA) and a quantitative assessment of the acne severity using the hyperspectral imaging method supported by image analysis and processing methods were used. Patients/Method: The acne lesion perfusion was compared to the healthy skin perfusion of the control group. The reflectance in the range from 400 to 1000 nm was also compared for acne lesions and healthy skin. A dedicated version of the algorithm based on the gray‐level co‐occurrence matrix was used to compare differences between acne lesions and healthy skin. Results: Blood perfusion in acne lesions is on average 117% higher than in healthy skin. The reflectance of acne lesions was lower in the range of 400–600 nm compared to the healthy skin. A statistically significant decrease in the reflectance of acne lesions was demonstrated for wavelengths: 434, 549, and 588 nm. Conclusions: Increased blood perfusion in acne lesions compared to healthy skin suggests increased chemotaxis of inflammatory cells and wound healing. Decrease points in skin reflectance resemble the absorption peaks for hemoglobin and coproporphyrin III produced by Cutibacterium acnes. Thus, the proposed methods may serve not only for the early detection of acne lesions, before they manifest themselves clinically for the naked eye, but may also be a tool for quantifying the effectiveness of acne treatment. [ABSTRACT FROM AUTHOR]

Background: Solar radiation is responsible for changes in the structure of human hair, the damages include proteins (65%–95%), lipids, and melanin. The aim was to examine the effectiveness of sunscreen in hair cosmetics and whether hair color affects it. Materials and Methods: The study included nine women, divided according to hair color to three groups: light, dark, and gray hair. The 410‐Solar reflectometer was used in five time points. The hair was divided into three strands, one product applied to each. Results: Dark hair showed the highest absorption of radiation in all wavelength ranges, the reflectance before products application was significantly higher than the hair reflectance immediately after application. The effect of sunscreens on light hair reflectance was found at wavelengths 400 and 720 nm and between 1000 and 2500 nm, the reflectance before application was significantly higher than the reflectance after. The use of products on gray hair did not have a significant effect on hair reflectance at wavelengths 400–1100 nm, the effect of sunscreens on the gray hair reflectance was observed in the UV and infrared range, the reflectance before application was significantly higher than immediately after. Conclusions: The results showed that the 410‐Solar reflectometer is useful to assess the effectiveness of hair sunscreens. All three tested hair products do not show the expected protection properties. Dark hair showed the highest absorption of radiation in all wavelength ranges, suggesting that dark hair should be more protected against radiation than light and gray hair. [ABSTRACT FROM AUTHOR]

Background: The mechanism of intense pulsed light action on the skin is based on selective photothermolysis. The light delivered to the tissue is scattered and absorbed by chromophores that absorb a beam of radiation of a specific length. The skin reflectance changes depending on the physiological state of the tissue, as shown by the hyperspectral camera. The aim of the study was to assess the hyperspectral reflectance of acne skin before and after intense pulsed light (IPL) therapy and to compare it with the reflectance of healthy skin. Materials and methods: The study involved 27 volunteers with diagnosed moderate acne. The control group consisted of 20 people without acne lesions. All acne volunteers underwent a series of four treatments using IPL at weekly intervals. The volunteers with acne lesions were photographed before the series of treatments and a week after the 4th treatment. Results: Acne skin shows lower reflectance than healthy skin. Acne skin after IPL therapy is characterized by a higher reflectance compared to acne skin before the therapy and resembles the reflectance of the skin of the control group. A statistically significant difference was found between the acne skin before the treatments and the skin of the control group. Conclusions: The effect of IPL therapy on acne skin is the increase of its reflectance by reducing the number of chromophores, which brings it closer to the reflectance value of healthy skin. Hyperspectral imaging allows for: the evaluation of the treated skin at each stage, a precise selection of the light wavelength depending on the problem, and therefore, for optimizing the number of irradiations and increasing the safety of the therapy. [ABSTRACT FROM AUTHOR]

Background: There is no method that can guarantee effective, quick, and noninvasive removal of tattoo dyes. Laser methods are considered to be the method of choice. In this study, an attempt was made to determine the in vitro spectral characteristics of selected dyes used in permanent makeup and tattoos and to analyze the obtained parameters in terms of laser treatments optimization. Materials and methods: Hyperspectral analysis was performed to determine the spectral characteristics of the dye on the entire surface of the slide. Seven dyes used in permanent makeup and tattoos were analyzed in vitro. The maximum reflectance and the wavelength for a given dye were determined for the maximum reflectance in the studied wavelength range: 400–1000 nm. The optical properties of the dyes were determined based on visible light imaging using camera. Results: The maximum radiation reflectance ranges from 634 to 732 nm for the tested dyes. Visually very similar colors may differ significantly in the wavelength for which the maximum absorption of the radiation occurs. White and yellow dyes are characterized by the highest reflectance value. The black dye is characterized by the lowest reflectance coefficient. Low reflectance of black dye results in more safe and effective removal treatments. Conclusion: The homogeneity of radiation absorption can be identified using methods of analysis and processing of images in visible light. Optimization of the wavelength of which the maximum absorption/reflectance of radiation occurs may allow us to increase the effectiveness of laser treatments for removing permanent makeup and tattoos. [ABSTRACT FROM AUTHOR]

Background: In the treatment of acne, skin parameters such as sebum secretion, hydration, and the content of hemoglobin and melanin are very important. The values of these parameters at the appropriate level show the maintenance of good functionality of the epidermal barrier and the impact of a given treatment procedure on the condition and physiology of the epidermis. Aims: The aim of the study was to evaluate the effectiveness of anti‐acne therapy with IPL by volunteers, as well as to quantify parameters such as sebum secretion, hydration level, and the content of melanin and hemoglobin in the skin. Patients/Methods: The study involved 27 volunteers with moderate acne. A series of 4 treatments was performed with intense pulse light at weekly intervals. The secretion of sebum, skin hydration, and the content of melanin and hemoglobin was measured. Results: A decrease in sebum secretion in the forehead area after light treatments was observed, the level of hydration remained the same in all areas examined, as did the levels of melanin and hemoglobin. The volunteers were asked about the satisfaction with the effects of the treatment: 100% answered that they were satisfied with the effects of the treatment to a degree of 7.8 ± 1.5. Conclusions: During anti‐acne therapy, therapists usually focus only on reducing acne lesions, forgetting that proper care has a large impact on the success of dermatological therapies. It is very important to know the influence of treatment procedures on the parameters of the skin in order to choose the right care for the best treatment effect. [ABSTRACT FROM AUTHOR]

Topical and oral antibiotic therapy is also a popular method of treatment. The effectiveness of this method is limited by the increasing resistance of bacteria to antibiotics. Over the decades since the introduction of antibiotics to treat acne, the resistance levels of bacteria have changed. This defense mechanism is developed evolutionarily. Modifications of antibiotic receptor sites, alteration of drug influx/efflux, or enzymatic degradation are common mechanisms used by bacteria to initiate and strengthen internal antibiotic resistance. The basic chromophores used in light therapy are hemoglobin, melanin, water bound to proteins, and porphyrins. Hemoglobin absorbs light mainly at 580 nm, while melanin absorbs the entire visible spectral range (400–750 nm). Porphyrins are aromatic compounds, classified as photosensitizing substances, intensively absorbing blue light, and to a lesser extent in long visible bands, such as orange and red light. Using IPL makes it possible to cover the maximum light absorption of porphyrins and hemoglobin, therefore it can be an effective tool in the treatment of inflammatory lesions in acne vulgaris. In view of the effectiveness of light therapy and its effect even on antibiotic‐resistant bacteria, it is worth considering the possibility of using light therapy instead of antibiotic therapy. Due to the increasing resistance of bacteria to antimicrobials, they should be used with caution and as a last resort. The high‐energy light treatment act only locally (unlike with antibiotics taken orally) and on a chromophore, such as melanin, hemoglobin, or porphyrins. [ABSTRACT FROM AUTHOR]

BACKGROUND: Hallux valgus, one of the most common foot disorders, contributes to the formation of pain and changes foot biomechanics. OBJECTIVE: To assess the impact of Kinesiology Taping (KT) on foot loading during gait in patients with hallux valgus. METHODS: Forty feet with hallux valgus were examined. Patients wore the KT for a month and the parameters of the foot during gait on a baropodometric platform were measured three times: before taping, immediately after application of taping and after one month's use. RESULTS: The taping had a statistically significant effect on dynamic foot measurements. The maximum and mean foot load (p < 0.001), foot surface (p < 0.001), ratio of forefoot to hindfoot load (p < 0.01) and the proportions of the lateral and medial foot loading (p < 0.05) all changed. During gait cycle, taping significantly increased the load and surface at the first metatarsal head (p < 0.001) while there was a decrease around the second to fifth (p < 0.001) metatarsal heads. CONCLUSIONS: Using KT to correct a hallux valgus is a procedure that has an impact on the dynamic parameters of the foot during gait. The use of this method could become an alternative to surgical treatment for those patients, who have any contraindication for surgery. [ABSTRACT FROM AUTHOR]

Summary: In recent years, popularity of radiofrequency (RF) has increased significantly. They are characterized by a low risk of complications and relatively high effectiveness. RF use high‐frequency currents causing oscillating motion of ions resulting in temperature rise stimulating skin regeneration processes. The aim of this work was the thermographic evaluation of the skin exposed to RF of different intensity. The dynamic thermal imaging was used to study the temperature of the skin exposed to RF. The research was carried out in two locations with different adipose tissue content: abdomen (ROI1) and forearm (ROI2). In the ROI1 area, RF was applied at nominal power range from 250 to 1750 W, while in ROI2 area: from 250 to 1000 W. The obtained thermographic data were fitted to exponential functions. A clear dependence of obtained thermokinetic parameters with the anatomical location of exposure to RF was demonstrated. Thicker layer of adipose tissue directly under the skin resulted in obtaining higher maximum temperatures of the skin surface during the procedure (maximum obtained temperature equaled 40.8°C). The temperature of the skin under the head of the device does not translate to subjective patient experiences. In anatomic locations filled with less adipose tissue mass, tolerance to RF is much lower. The dynamics of skin temperature changes, after the RF treatment, can be described by means of a single exponential function where the key parameter is the time constant t1 defining the dynamics of skin temperature changes. The depth of the RF influence is slightly correlated with the RF power. [ABSTRACT FROM AUTHOR]

Introduction: Patient compliance with care recommendations is closely related to the role of cosmetics in delaying or preventing the side effects of medications used to treat acne. Methods: A total of 33 volunteers with symptoms of acne vulgaris were qualified for the study. The skin parameters were measured before the experiment, after the 4 and at the 8 weeks of use. The measurements were subject to moisturizing the skin and secretion of sebum and skin color. A skin acoustic parameters using high‐frequency ultrasound (33 MHz) was also performed. The recorded images were used to determine the epidermal entrance echo thickness and echo density of dermis. Results: The results show a change in the value of skin parameters during the 8‐week application of a specific skin care procedure. The secretion of sebum did not change significantly, there was a decrease in hydration, and the level of melanin and hemoglobin remained at the same level. The thickness of the epidermis entrance echo in the ultrasound image was thinned. Conclusion: For many mild/moderate acne patients, cosmetics proven to be effective and well tested in clinical trials can offer a real benefit in reducing both inflammation, sebum production, melanin, and irritation, but should also be moisturizing. [ABSTRACT FROM AUTHOR]

Abstract The practice of drug counterfeiting is an important challenge due to the extremely rapid growth rate of this disturbing trend and its immense potential harmfulness. According to WHO even 10% of counterfeit medicines can be exact copies of genuine medicines: they have exactly the same quantitative and qualitative composition in terms of both API and excipients. Thus, the identification of such drugs using chemical analysis methods can be very difficult or even impossible. The aim of this study was to verify the effectiveness of using computed microtomography in the identification of counterfeit medicines. The General Electric v|tome|x s microtomography system was used in the study. The recorded microtomographic scans were subjected to analysis and image processing. The following parameters of image analysis and processing were identified: mean brightness, homogeneity, contrast, quadratic tree decomposition. The original and falsified 100-mg Viagra® tablets (Pfizer) were compared. 8 original Viagra® tablets (hereinafter referred to as T) and 8 falsified tablets (hereinafter referred to as F1-F8) were tested. The range of variation for the genuine medicines against fake products was: brightness: 90.9–117.1 vs 33.8, 50.1, homogeneity: 0.84–0.92 vs 0.94–1.01 and quadratic tree decomposition for the 1 × 1 mask: 55768–58792 vs 0–439. The proposed method of microtomographic image analysis and processing enables to identify solid dosage forms, including those that are an accurate chemical copy, with high sensitivity and specificity, 94.5% and 97%, respectively. The advantage of the μCT method is its high efficiency and speed, whereas the disadvantages include the possibility of using only solid dosage forms and high equipment costs. Graphical abstract fx1 Highlights • μCT enables to identify counterfeit medicines with high sensitivity and specificity. • Brightness, homogeneity and QTDECOMP differentiate fake/original medicines. • μCT enables to identify chemical copies of original medicines. • Disadvantage of μCT: costs of μCT scanner; only for solid dosage forms. [ABSTRACT FROM AUTHOR]