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1. Flow cytometric analysis of benthic prokaryotes attached to sediment particles

Author

Amalfitano, Stefano and Puddu, Stefano Fazi, Alberto

Subjects
*PROKARYOTES, *BENTHOS, *NUCLEIC acid analysis, *MICROBIAL ecology, *RIVER sediments, *RIVER channels, *FLOW cytometry, *CELL membranes
Abstract

Abstract: Appropriate detachment treatments are required to analyze prokaryotes associated with streambed sediments by flow cytometry. Using our previously optimized protocol, two groups of cells exhibiting different nucleic acid contents were easily detectable. However, the Nucleic Acid Double Staining assay proved that detachment procedures negatively affect the cell membrane integrity. [Copyright &y& Elsevier]

Published
2009
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