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Motivated by the image reconstruction in spatiotemporal imaging, we introduce a concept named diffeomorphic optimal transportation (DOT), which combines the Wasserstein distance with Benamouâ€"Brenier formula in optimal transportation and the flow of diffeomorphisms in large deformation diffeomorphic metric mapping. Using DOT, we propose a new variational model for joint image reconstruction and motion estimation, which is suitable for spatiotemporal imaging involving mass-preserving large diffeomorphic deformations. We further get its equivalent PDE-constrained optimal control formulation. The proposed model is easy-to-implement and solved by an alternating gradient descent algorithm, which is compared against existing alternatives theoretically and numerically. The performance is validated by several numerical experiments in spatiotemporal tomography, where the projection data is time-dependent sparse and/or high-noise. Moreover, we present several extensions based on DOT. Under appropriate conditions, the proposed algorithm can be adapted as a new algorithm to solve the models using quadratic Wasserstein distance. [ABSTRACT FROM AUTHOR]

Highlights • The method with photocaged probes for imaging was efficient and spatiotemporal. • The two probes NDPPI and NNDPPI could be applied in cells and human tissues with satisfactory results. • This strategy can be used in other modified reagents for controlling biological function with light. Abstract Two compounds with nitrobenzene-derived caging groups were designed and synthesized for cells and tissues imaging. After 350 nm irradiation, the two reagents can realize highly efficient fluorescence turn on with large Stokes shift. As for the two probes can bare out a common functional group of aldehyde group under UV irradiation, it may be used for detection of cysteine, DNA methylation in a spatiotemporal method with selectivity. The idea of design and synthesis might be used in other modified reagents for controlling biological function with light. [ABSTRACT FROM AUTHOR]

Abstract: Diffuse acoustic or seismic waves are highly sensitive to detect changes of mechanical properties in heterogeneous geological materials. In particular, thanks to acoustoelasticity, we can quantify stress changes by tracking acoustic or seismic relative velocity changes in the material at test. In this paper, we report on a small‐scale laboratory application of an innovative time‐lapse tomography technique named Locadiff to image spatiotemporal mechanical changes on a granite sample under biaxial loading, using diffuse waves at ultrasonic frequencies (300 kHz to 900 kHz). We demonstrate the ability of the method to image reversible stress evolution and deformation process, together with the development of reversible and irreversible localized microdamage in the specimen at an early stage. Using full‐field infrared thermography, we visualize stress‐induced temperature changes and validate stress images obtained from diffuse ultrasound. We demonstrate that the inversion with a good resolution can be achieved with only a limited number of receivers distributed around a single source, all located at the free surface of the specimen. This small‐scale experiment is a proof of concept for frictional earthquake‐like failure (e.g., stick‐slip) research at laboratory scale as well as large‐scale seismic applications, potentially including active fault monitoring. [ABSTRACT FROM AUTHOR]

Okadaic acid (OA) has attracted considerable attention in the fields of human health and public safety because it can cause severe virulence and is widely distributed. Therefore, it is of immediate significance to explore a novel method for highly sensitive, low-consumption, efficient, and convenient OA detection. In this study, a triple-helix molecular switch (THMS)-based light-addressable potentiometric aptasensor (LAPA) is proposed for the highly sensitive and selective label-free detection and spatiotemporal imaging of OA. In this method, a THMS containing an OA aptamer served as the recognition element, and a DNA microarray including a light-addressable potentiometric sensor was designed as the transducer. Meanwhile, poly (allylamine hydrochloride) was modified on the chip surface to enhance DNA absorption and signal detection. A detection platform was constructed to measure signals and scan spatiotemporal images. The realized biosensing system enabled the multichannel detection of OA in a linear range of 0.05–100 nM with low sample consumption, a detection limit of 0.03 nM, and a detection time of 27 min/channel and spatiotemporal imaging analysis with a resolution of 400 µm2/pixel and frame duration of 3.2 min/frame. Considering the technical developing of aptamer-based THMS and micro/nano biosensor, the proposed method will provide a promising approach for label-free detection and spatiotemporal analysis of OA with high sensitivity, specificity, and detection efficiency; low sample dosage; and simple operation. • The THMS-based LAPA realizes unlabeled detection and spatiotemporal analysis of OA. • The THMS-based aptamer improves the sensitivity and selectivity of biosensor. • The LAPS implements multi-channel detection and spatiotemporal imaging. • The PAH modification improves the output signal quality of the transducer. • The self-built platform scans the weak photocurrent automatically and efficiently. [ABSTRACT FROM AUTHOR]

In this study, we successfully determined spatiotemporal distribution of curcumin in mice via simple and fast fluorescence detection of native curcumin and stabilized curcumin. We used 2-aminoethyl diphenyl borate (DPBA) as a stabilizer of curcumin, which binds to curcumin and enhances its aqueous stability. After intravenous injection, curcumin and DPBA–curcumin complexes showed similar fluorescence intensities in the brain, pancreas, lungs, and kidneys at 15 min. However, stabilized DPBA–curcumin complexes exhibited much stronger fluorescent signals at metabolically active sites such as liver tissues than native curcumin. After incubation for 1–3 h, native curcumin showed significantly rapid reduction of fluorescent signals, compared to DPBA–curcumin complexes, probably due to degradation and reduction. In addition, complicate extraction procedures inhibited precise fluorescent monitoring of unstable curcumin, which result in different biodistribution of curcumin before and after extraction. Direct fluorescent monitoring could allow evaluation of in vivo distribution and fate of curcumin, which could be also applied to diverse natural polyphenols with fluorescent signals. [ABSTRACT FROM AUTHOR]