1. Morpho-structural properties of ZnSe, TiO2-ZnSe materials and enzymatic activity of their bioinorganic hybrids with lysozyme.
- Author
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Anastasescu, C., Gifu, I.C., Negrila, C., Socoteanu, R., Atkinson, I., Calderon-Moreno, J.M., Munteanu, C., Plavan, G., Strungaru, S.A., Cheatham, B., Malaroiu, A.-V., Teodorescu, V.S., Anastasescu, M., Zaharescu, M., Balint, I., and Lazarescu, V.
- Subjects
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LYSOZYMES , *ZINC selenide , *ENZYMES , *SURFACE properties , *ZINC catalysts - Abstract
[Display omitted] • Micro-sized ZnSe and TiO 2 -ZnSe spheres were obtained by hydrothermal method. • TiO 2 addition induces structural and physical–chemical changes to zinc selenide material. • ZnSe and TiO 2 -ZnSe surface properties were investigated before and after lysozyme adsorption. • TiO 2 -ZnSe proved higher lysozyme loading capacity than bare ZnSe. • The hydrolytic activity of Lys/ZnSe and Lys/TiO 2 -ZnSe is twofold higher than for free lysozyme. Structural and functional changes induced by TiO 2 addition to spherical zinc selenide together with lysozyme adsorption lead to valuable bioinorganic catalysts with enhanced activity. Spherical zinc selenide and its composite with TiO 2 were obtained by hydrothermal method and subsequently modified by lysozyme adsorption. Morphological, structural and functional characteristics of zinc selenide based materials (ZnSe, TiO 2 -ZnSe) and their hybrids with lysozyme (Lys/ZnSe, Lys/TiO 2 -ZnSe) were investigated. TiO 2 addition to ZnSe results in significant changes of surface composition, electrochemical behavior and lysozyme retention capacity. The lysozyme modified ZnSe and TiO 2 -ZnSe surfaces doubles the biocatalysts efficiency for 4-Methylumbelliferyl β-D-N,N′,N″-triacetylchitotrioside hydrolysis reaction compared to free lysozyme. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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