Search

Nickel (Ni) is an essential micronutrient known for improving the growth and yield of various crops at low concentrations. However, the nickel’s adequate information on shallots is insufficient. This study found out the effect of Ni fertilization with four concentrations (0.0, 0.025, 0.1, and 0.4 mg L -1 derived from NiSO4.6H2O) on plant and bulb weight, nutrient solution usage, and bulb nickel and nitrate content of two shallot cultivars (Lokananta and Sanren F1) using hydroponic condition, arranged in a split-plot design under field conditions. Increased Ni concentration (0.4 mg L -1 ) negatively affected plant height, total plant weight, bulb weight per plant, and total nutrient solution usage, while it increased the bulb nickel and leaf carotenoid contents. The Ni concentration (0.025 mg L -1 ) can be safer for shallot plant absorption with optimum growth and yield. Shallot cultivar Sanren F1 provides considerably better results in terms of yield components than cultivar Lokananta. Bulb weight per plant appeared positively correlated with the number of leaves, bulbs, total bulb diameter, and total volume of nutrient solution used per plant. The total bulb diameter per plant showed a better direct effect on the shallot bulb yield at various Ni levels. [ABSTRACT FROM AUTHOR]

A field experiment was conducted on Chromolaena odorata dominated pasture with the objectives of evaluating efficacy of integrated of mechanical and cultural control on weed suppression and determining botanical composition of plant species after treatment started. The treatments were, T1 slashing of Chromolaena every month, T2 digging up of Chromolanea, and exposed to dry (mulching), T3 digging up of Chromolaena followed by burning, T4 digging up of Chromolaena followed by burning and sowing with Centrosema pubescens and T5 digging up of Chromolaena followed by burning and planting with Brachiaria decumbens. Results of experiment showed that under drought conditions, digging up Chromolaena was very effective in suppressing regrowth of Chromolaena but it was not effective to other weeds. Among treatments, digging out of Chromolaena followed by burning and planting with Brachiaria decumbens was the most effective and slashing of Chromolaena every month was the least effective in suppressing weeds. Botanical composition was shifted with treatments. Stachytarpheta jamaicensis and Chromolaena were the dominant species in monthly slashed Chromolaena plots, Stachytarpheta and Calopogonium muconoides were the dominant species in mulched plots while Mimosa pudica, Brachiaria and Centrosema were the dominant species in burnt plots. [ABSTRACT FROM AUTHOR]

The betaherpesvirus human herpesvirus 6 (HHV-6) has two variants. The U83 gene product of strain HST is a chemoattractant for monocytes. Here, we describe U83 gene variations that accumulated in variants A and B. A gene-variation hot spot was examined in 36 different strains and one donor DNA sample. U83 gene variations accumulated in variant A and in reactivated variant B after transplantation. None of the variant-A viruses encoded the signal peptide found in the B variant. U83 gene sequencing suggested that the variant A and B groups were separate, and that the variant B viruses could be further divided into the HST-Z29 type and another type with a shorter signal peptide. In a eukaryotic expression system, the HST-Z29 type of U83 gene product was secreted into the medium, a frame-shifted HST-Z29 type was partially secreted, and the variant-A type and a first-methionine knockout of the HST-Z29 type were not secreted. [ABSTRACT FROM AUTHOR]

World Health Organization (WHO) placed ESBL producing Klebsiella pneumoniae in the Priority Pathogen List as one of the first priority that pose great threat to human health. Therefore we intend to discover the ESBL producing K.pneumoniae with both phenotype and genotype characteristics. [Extracted from the article]

The surveillance of multidrug-resistant (MDR) H58 typhoid is highly important, especially in endemic areas. MDR strain detection is needed by using a simple PCR technique that only uses a pair of primers. This is conducted considering the detection of Salmonella Typhi strains that have been carried out so far are only using antimicrobial sensitivity tests to determine microbial resistance phenotypically and to determine genotypically using complex molecular techniques. We aimed to analyse the existence of Salmonella Typhi MDR H58 in patients with typhoid fever in Makassar, Indonesia. A total of 367 blood samples of typhoid fever patients were collected from April 2018 until April 2019. The blood sample was cultured, then confirmed via simple PCR. All of the confirmed samples were tested for susceptibility against antibiotics and molecularly analysed for MDR H58 existence using a simple PCR technique. We found 7% (27/367) of the samples to be positive by both blood culture and PCR. All 27 isolates were found to be sensitive to sulfamethoxazole/trimethoprim. The lowest drug sensitivities were to amoxicillin, at one (3.7%) of 27 isolates, and ampicillin, at 13 (48.1%) of 27 isolates. Salmonella Typhi H58 PCR results showed that one (3.7%) of 27 isolates carried a positive fragment of 993 bp that led to the H58 strain, since the deletion flanks this fragment. The isolate was also found to be resistant to amoxicillin and fluoroquinolone according to a sensitivity test. Further molecular analysis needs to be conducted to examine the single isolate that carried the 933 bp fragment. [ABSTRACT FROM AUTHOR]