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The mental health of children in public care has received considerable attention in recent years. There are significant differences in the prevalence rates compared to children living at home and not in public care and these are added to by other adverse lifestyle issues. Considerable attention has been focused on the importance of stable and secure placements supported by access to a range of services including education, health and mental health. Identifying and classifying mental health needs proves to be challenging as the child’s genetic inheritance, pre- and post-birth experiences, including maltreatment, interact with the uncertainty and delays commonly associated with child protection processes, action in the legal system and the difficulty in establishing secure and permanent placements. Clinical need is also related to the age of the child and the reasons they came into care and the length of time they remain. A case illustrating many of these issues, publicly reported in the court’s judgement, is used as the basis for exploring the complexity for the identified child in understanding and making sense of their experiences as placement plans are made for them. These are used to explore the difficulties in establishing clinical protocols such as those recently published in the USA. Particular emphasis is given to the issues faced by children and their carers in establishing new family relationships which address the child’s inheritance from their family of origin especially where these are traumatic and conflicted in origin. [ABSTRACT FROM PUBLISHER]

Attachment theory and therapy have established themselves as major constructs in foster care and adoption practice. The depth of the explanatory power of attachment theory and research have done much to both identify the source of developmental difficulties in adopted and fostered children, and of the significant changes that can be made when these children are placed with `autonomously' classified carers. However, the power of the concept has also led to a proliferation of ideas, particularly therapeutic models that can, in some cases, stray a long way from the core of what is effective, safe and ethical clinical practice. Holding therapy is one of these models. The British Association for Adoption and Fostering developed a position statement on this practice, setting it within a broader framework of assessment and intervention. But like the model, the development of the position statement became controversial in its development. The article charts some of the critical issues and identifies the very significant dilemmas for parents and carers caught up in the maelstrom of challenging behaviours and disturbing feelings. The conclusion turns to one of the key components of an attachment-based idea: The critical importance of finding the space to think and to feel what the child is communicating without resorting to dangerous retaliation. [ABSTRACT FROM AUTHOR]

A wheat germin gene, with oxalate oxidase (OxO) activity, can be used as a sensitive reporter gene in both monocot and dicot transformations. Detection of H2O2 generated from OxO oxidation of oxalate provides simple, rapid detection of gene expression. Inexpensive substrates are required for both assays. OxO activity, could be detected histochemically in minutes, without chlorophyll clearing procedures. This assay was used to optimize transformation procedures and to track stable transgene expression in breeding populations over many generations. A simple spectrophotometric quantitative enzyme activity assay was used to select lines with various levels of transgene expression and to monitor transgene silencing phenomena. The quantitative OxO assay can also be used as an internal DNA delivery standard with a second reporter gene used in gene expression studies. The simplicity of the assay is ideal for screening large populations to identify primary transgenics, for monitoring transgene segregation in large populations in field studies and for assessing stability of transgene expression over numerous generations. [ABSTRACT FROM AUTHOR]

The present study aimed to explore the impact of either being separated or adopted with sibling(s) on adolescent adoptees' emotional and behavioral development. Through a mixed method design, the study aimed to explore whether sibling placement had an effect on adolescents' internalizing and externalizing problems, as well as on the way they express and regulate their emotions in conflictual situations. Findings revealed that, although both groups of adoptees developed adaptive strategies to cope with challenging events, those placed with siblings were in general faring better than those placed alone. Being placed alone was, indeed, associated with clinical scores on the self-reported Youth Self Report (YSR) Total Problems' scale and to the greater use of maladaptive emotion regulation strategies. This highlights the significance of maintaining siblings' relationships when co-placement is not possible. [ABSTRACT FROM AUTHOR]

A highly efficient method of regenerating fertile, phenotypically normal plants from shoot apex cultures of T. aestivum was developed. The hypodermal layer (L2) of the vegetative apex containing germ line precursor cells could be located with bright field microscopy and targeted for microinjection. Fluorescently labelled dextrans were used as markers to develop a microinjection procedure which did not disrupt nuclear or cytoplasmic structure. This procedure was used to inject plasmid DNA into L2 cells. Capillary microinjection did not shear the plasmid DNA and delivery of DNA was confirmed by polymerase chain reaction analysis of DNA isolated from injected apices. The significance of these findings in relation to the development of cereal transformation systems will be discussed. [ABSTRACT FROM AUTHOR]

In vitro responses can be influenced by the developmental status of the donor plant tissue. The effects of the donor plant photoperiod and the developmental stage of the plant on organogenesis of petiole explants of Begonia × hiemalis cv. Schwabenland Pink are reported. Long day plants had progressively more branches, total stem length, leaf area, and branch and shoot mass. In short days, flowering was earlier and a greater proportion of dry weight accumulation was allocated to reproductive structures. Similar explant responses were obtained from all developmental stages until flowering was well advanced in short days and then the regenerative capacity diminished. Primary measurements of donor leaves (length, area and weight) at the time of explant removal were not well correlated with adventitious bud production. Loss of regeneration was not determined by tissue or plant age but was associated with the progressive development of flowers. Thus the donor plant photoperiod only indirectly affected bud production. Organogenesis decreased with duration of short days but increased in long days and thus both the duration of the multiplication phase and the intensity of the in vitro response was enhanced by maintaining donor plants in long days. [ABSTRACT FROM AUTHOR]

Editorial. Introduces articles featured in the July 2003 issue of 'Clinical Child Psychology and Psychiatry.'

Attachment theory and research are drawn upon in many applied settings, including family courts, but misunderstandings are widespread and sometimes result in misapplications. The aim of this consensus statement is, therefore, to enhance understanding, counter misinformation, and steer family-court utilisation of attachment theory in a supportive, evidence-based direction, especially with regard to child protection and child custody decision-making. The article is divided into two parts. In the first, we address problems related to the use of attachment theory and research in family courts, and discuss reasons for these problems. To this end, we examine family court applications of attachment theory in the current context of the best-interest-of-the-child standard, discuss misunderstandings regarding attachment theory, and identify factors that have hindered accurate implementation. In the second part, we provide recommendations for the application of attachment theory and research. To this end, we set out three attachment principles: the child's need for familiar, non-abusive caregivers; the value of continuity of good-enough care; and the benefits of networks of attachment relationships. We also discuss the suitability of assessments of attachment quality and caregiving behaviour to inform family court decision-making. We conclude that assessments of caregiver behaviour should take center stage. Although there is dissensus among us regarding the use of assessments of attachment quality to inform child custody and child-protection decisions, such assessments are currently most suitable for targeting and directing supportive interventions. Finally, we provide directions to guide future interdisciplinary research collaboration. [ABSTRACT FROM AUTHOR]

Attachment theory and research are drawn upon in many applied settings, including family courts, but misunderstandings are widespread and sometimes result in misapplications. The aim of this consensus statement is, therefore, to enhance understanding, counter misinformation, and steer family-court utilisation of attachment theory in a supportive, evidence-based direction, especially with regard to child protection and child custody decision-making. This article is divided into two parts. In the first part, we address problems related to the use of attachment theory and research in family courts, and discuss reasons for these problems. To this end, we examine family court applications of attachment theory in the current context of the best-interest-of-the-child standard, discuss misunderstandings regarding attachment theory, and identify factors that have hindered accurate implementation. In the second part, we provide recommendations for the application of attachment theory and research. To this end, we set out three attachment principles: the child's need for familiar, non-abusive caregivers; the value of continuity of good-enough care; and the benefits of networks of attachment relationships. We also discuss the suitability of assessments of attachment quality and caregiving behaviour to inform family court decision-making. We conclude that assessments of caregiver behaviour should take center stage. Although there is dissensus among us regarding the use of assessments of attachment quality to inform child custody and child-protection decisions, such assessments are currently most suitable for targeting and directing supportive interventions. Finally, we provide directions to guide future interdisciplinary research collaboration. [ABSTRACT FROM AUTHOR]

Pretty good yield (PGY) is a sustainable fish yield corresponding to obtaining no less than a specified large percentage of the maximum sustainable yield (MSY). We investigated 19 European fish stocks to test the hypothesis that the 95% PGY yield range is inherently precautionary with respect to impairing recruitment. An FMSY range was calculated for each stock as the range of fishing mortalities (F) that lead to an average catch of at least 95% of MSY in long-term simulations. Further, a precautionary reference point for each stock (FP.05) was defined as the F resulting in a 5% probability of the spawning-stock biomass falling below an agreed biomass limit below which recruitment is impaired (Blim) in long-term simulations. For the majority of the stocks analysed, the upper bound of the FMSY range exceeded the estimated FP.05. However, larger fish species had higher precautionary limits to fishing mortality, and species with larger asymptotic length were less likely to have FMSY ranges impairing recruitment. Our study shows that fishing at FMSY generally is precautionary with respect to impairing recruitment for highly exploited teleost species in northern European waters, whereas the upper part of the range providing 95% of MSY is not necessarily precautionary for small- and medium-sized teleosts. [ABSTRACT FROM AUTHOR]

Background While studies of ex-orphanage care show adverse effects on development, the longer-term impact on mid-life psychosocial functioning and physical health has not been established. Methods Orphanage records provided baseline data on a sample of 100 Hong Kong Chinese girls who were subsequently adopted into the UK. A mid-life follow-up using standardised questionnaires and face-to-face interviews assessed current circumstances, life satisfaction and mental and physical health outcomes. Comparisons were made with age-matched UK-born adopted and nonadopted women. Results Half the group spent between 1 and 2 years in orphanages, average age at adoptive placement was 23 months and 72% participated in the follow-up. Despite this poor early start, mid-life outcomes were commensurate with the comparison groups in terms of mental and physical health measures. Serious psychiatric and social difficulties were largely absent. Although the timing and extent of exposure to orphanage care did not influence outcome, participants' reports of poorer quality adoptive family experience and a negative view of their adoption were significantly associated with poorer mental health outcomes (difference in means = 0.76, 95% CI 1.33-0.19, p = .01; difference in means = 1.2, 95% CI 0.68-1.73, p = .01, respectively). Conclusions Moderately depriving orphanage care did not predict enduring adverse consequences in mid-life but subsequent poor adoption experience was associated with outcome. [ABSTRACT FROM AUTHOR]

Cold-induced inhibition of CO2 assimilation in maize (Zea mays L.) is associated with a persistent depression of the photochemical efficiency of PSII. However, very limited information is available on PSI photochemistry and PSI-dependent electron flow in cold-stressed maize. The extent of the absorbance change (ΔA820) used for in vivo quantitative estimation of photooxidizable P700+ indicated a 32% lower steady-state oxidation level of the PSI reaction center P700 (P700+) in cold-stressed compared with control maize leaves. This was accompanied by a 2-fold faster re-reduction rate of P700+ in the dark, indicating a higher capacity for cyclic electron flow (CEF) around PSI in cold-stressed maize leaves. Furthermore, the increased PSI-dependent CEF(s) was associated with a much higher stromal electron pool size and 56% lower capacity for state transitions compared with control plants. To examine NADP(H) dehydrogenase (NDH)- and ferredoxin:plastoquinone oxidoreductase (FQR)-dependent CEF in vivo, the post-illumination transient increase of Fo′ was measured in the presence of electron transport inhibitors. The results indicate that under optimal growth conditions the relatively low CEF in the maize mesophyll cells is mostly due to the NDH-dependent pathway. However, the increased CEF in cold-stressed plants appears to originate from the up-regulated FQR pathway. The physiological role of PSI down-regulation, the increased capacity for CEF and the shift of preferred CEF mode in modulating the photosynthetic electron fluxes and distribution of excitation light energy in maize plants under cold stress conditions are discussed. [ABSTRACT FROM PUBLISHER]

Cytochrome f from the psychrophile Chlamydomonas raudensis UWO 241 has a lower thermostability of its c-type heme and an apparent molecular mass that is 7 kDa lower than that of the model mesophilic green alga Chlamydomonas reinhardtii. We combined chloroplast transformation, site-directed mutagensis, and the creation of chimeric fusion constructs to assess the contribution of specific domains and (or) amino acids residues to the structure, stability, and accumulation of cytochrome f, as well as its function in photosynthetic intersystem electron transport. We demonstrate that differences in the amino acid sequence of the small domain and specific charged amino acids in the large domain of cytochrome f alter the physical properties of this protein but do not affect either the thermostability of the c-type heme, the apparent half-life of cytochrome f in the presence of the chloroplastic protein synthesis inhibitor chloramphenicol, or the capacity for photosynthetic intersystem electron transport, measured as e–/P700. However, pulse-labeling with [14C]acetate, combined with immunoblotting, indicated that the negative autoregulation of cytochrome f accumulation observed in mesophilic C. reinhardtii transformed with chimeric constructs from the psychrophile was likely the result of the defective association of the chimeric forms of cytochrome f with the other subunits of the cytochrome b6/f complex native to the C. reinhardtii wild type. These results are discussed in terms of the unique fatty acid composition of the thylakoid membranes of C. raudensis UWO 241 adapted to cold environments. [ABSTRACT FROM AUTHOR]