16 results on '"Shen, Manli"'
Search Results
2. Microwave hydrothermal-assisted preparation of novel spinel-NiFe2O4/natural mineral composites as microwave catalysts for degradation of aquatic organic pollutants.
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Shen, Manli, Fu, Lu, Tang, Jianhua, Liu, Mingyu, Song, Youtao, Tian, Fangyuan, Zhao, Zhigang, Zhang, Zhaohong, and Dionysiou, Dionysios D.
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NICKEL catalysts , *CATALYTIC activity , *MICROWAVES , *CHEMICAL decomposition , *HYDROTHERMAL synthesis , *SULFONATES - Abstract
In this study, novel spinel-NiFe 2 O 4 /natural mineral (sepiolite, diatomite and kaolinite) composites were developed using microwave (MW) hydrothermal method, and applied in MW-induced catalytic degradation (NiFe 2 O 4 /natural mineral/MW) of organic pollutants such as sodium dodecyl benzene sulfonate (SDBS), azo fuchsine (AF), methyl parathion (MP), and crystal violet (CVL) in solution. Catalytic activities of three NiFe 2 O 4 /natural mineral composites were compared. The effects of material synthesis process parameters such as molar ratios of NiFe 2 O 4 and natural mineral, and pH of precursor solutions for synthesizing catalysts, and degradation parameters such as MW irradiation time and catalyst reuse cycles were also investigated. The principle on NiFe 2 O 4 /natural mineral/MW degradation was provided. The results reveal that organic pollutants in wastewater can be removed completely using NiFe 2 O 4 /natural mineral/MW within minutes. NiFe 2 O 4 /sepiolite shows higher catalytic activity than the others. The calculated degradation rate constants are 1.865, 0.672, 0.472, and 0.329 min −1 for SDBS, AF, MP, and CVL, respectively, using NiFe 2 O 4 /sepiolite/MW system. The performance of NiFe 2 O 4 /natural mineral can be maintained for three reuse cycles. Active species OH, O 2 − , and h + play main roles in NiFe 2 O 4 /sepiolite/MW degradation. Hence, NiFe 2 O 4 /sepiolite/MW technology with rapid and cost-effective degradation, magnetic separation, and no secondary pollution, demonstrates to be promising in treating organic contaminants in wastewater. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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3. Antibacterial and immunoregulatory activity of an antimicrobial peptide hepcidin in loach (Misgurnus anguillicaudatus).
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Jin, Zeyu, Shen, Manli, Wang, Liuen, Wang, Cui, Gao, Miaomiao, Yu, Guoying, Chang, Zhongjie, and Zhang, Xiaowen
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ANTIMICROBIAL peptides , *ANTIBACTERIAL agents , *BACTERIAL cell membranes , *ANTI-infective agents , *PEPTIDES , *HEPCIDIN , *PEPTIDE antibiotics , *ANIMAL feeds - Abstract
Antimicrobial peptides (AMPs) are members of humoral immunity and particpate in resisting microbial invasion. In this study, an AMP gene hepcidin was obtained from the oriental loach Misgurnus anguillicaudatus and named Ma-Hep. This Ma-Hep encodes a peptide of 90 amino acids, with a predicted active peptide segment (Ma-sHep) of 25 amino acids at C terminus. Stimulation by a bacterial pathogen Aeromonas hydrophila resulted in significant up-regulation of Ma-Hep transcripts in loach midgut, head kidney, and gill. Ma-Hep and Ma-sHep proteins were expressed in Pichia pastoris and their antibacterial activity was examined. Results showed that Ma-sHep possessed stronger antibacterial activity against various Gram-positive and Gram-negative bacteria, compared to Ma-Hep. Scanning electron microscopy showed that Ma-sHep might kill bacteria by destroying bacterial cell membranes. Moreover, we found that Ma-sHep had an inhibitory effect on blood cell apoptosis induced by A. hydrophila and facilitated the bacterial phagocytosis and clearance in loach. Histopathological analysis indicated Ma-sHep could protect liver and gut of loach from bacterial infection. Ma-sHep has high thermal stability and PH stability, which is conducive to further feed addition. Feed supplemented with Ma-sHep expressing yeast improved the intestinal flora of loach by increasing the dominant bacteria and decreasing the harmful bacteria. Feed supplemented with Ma-sHep expressing yeast also regulated the expression of inflammatory related factors in various tissues of loach and reduced the mortality of loach upon bacterial infection. These findings show that the antibacterial peptide Ma-sHep is involved in the antibacterial defense of loach and can be used as a candidate for new antimicrobial agents in aquaculture. • The Hepcidin from Misgurnus anguillicaudatus was cloned and identified. • Ma-sHep possessed strong antibacterial activity against various bacteria. • Ma-sHep had an inhibitory effect on blood cell apoptosis induced by A. hydrophila. • Ma-sHep could protect loach tissues from bacterial infection. • Feed supplemented with Ma-sHep expressing yeast enhance immune capacity of loach. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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4. Impact of aluminum exposure on oxidative stress, intestinal changes and immune responses in red swamp crayfish (Procambarus clarkii).
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Zhang, Xiaowen, Shen, Manli, Wang, Cui, Gao, Miaomiao, Wang, Liuen, Jin, Zeyu, and Xia, Xiaohua
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- 2023
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5. Accumulation of polyethylene microplastics induces oxidative stress, microbiome dysbiosis and immunoregulation in crayfish.
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Zhang, Xiaowen, Jin, Zeyu, Shen, Manli, Chang, Zhongjie, Yu, Guoying, Wang, Lan, and Xia, Xiaohua
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CRAYFISH , *MICROPLASTICS , *PROCAMBARUS clarkii , *OXIDATIVE stress , *POLYETHYLENE , *DYSBIOSIS - Abstract
Microplastics have become a worldwide pollutant, widely discovered in soil, air and aquatic environment. Microplastics have been found in habitats where crayfish (Procambarus clarkii) cultivated, but the impact of microplastics on crayfish remains unclear. In this study, after 21-day dietary exposure, polyethylene (PE) particles were found to accumulate in intestine, hepatopancreas, gills and hemolymph of crayfish. Furthermore, PE particles can still be detected in these tissues after a 7-day depuration in clean water. PE retained in these tissues caused oxidative stress responses, as indicated by the change of oxidative-stress-related index, such as the increase of H 2 O 2 level and SOD activity. PE exposure also caused hemocytic encapsulation in crayfish hepatopancreas and increase of mucus secretion in intestine. Moreover, PE exposure affected the microbiota balance in crayfish, by reducing the total microbiota abundance and altering the proportions of many bacterial families. Interestingly, results showed that PE exposure led to of lower numbers of hemocytes and declination of phenoloxidase activity. Finally, PE exposure induced the expression of immune-related genes, including transcription factors and antimicrobial peptides. Taken these together, we conclude that PE microplastics exert considerable toxic effects on crayfish and are a potential threat to crayfish aquaculture and consumption. This study provides basic toxicological data toward quantifying and illuminating the impact of PE microplastics on freshwater animals. [Display omitted] • Polystyrene microplastics accumulated in several tissues of crayfish and caused histopathological changes. • Polystyrene microplastics induced oxidative stress and immune responses in crayfish. • Polystyrene microplastics induced gut microbiota dysbiosis in crayfish. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Clustering-local-unique-enriched-signals (CLUES) promotes identification of novel regulators of ES cell self-renewal and pluripotency.
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Wu, Chao, Jiao, Yang, Shen, Manli, Pan, Chen, Cheng, Guo, Jia, Danmei, Zhu, Jing, Zhang, Long, Zheng, Min, and Jia, Junling
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AUTOPOIESIS , *TRANSCRIPTION factors , *HISTONES , *SIGNAL processing , *CYTOLOGY - Abstract
Background: Key regulators of developmental processes can be prioritized through integrated analysis of ChIP-Seq data of master transcriptional factors (TFs) such as Nanog and Oct4, active histone modifications (HMs) such as H3K4me3 and H3K27ac, and repressive HMs such as H3K27me3. Recent studies show that broad enrichment signals such as super-enhancers and broad H3K4me3 enrichment signals play more dominant roles than short enrichment signals of the master TFs and H3K4me3 in epigenetic regulatory mechanism. Besides the broad enrichment signals, up to ten thousands of short enrichment signals of these TFs and HMs exist in genome. Prioritization of these broad enrichment signals from ChIP-Seq data is a prerequisite for such integrated analysis. Results: Here, we present a method named Clustering-Local-Unique-Enriched-Signals (CLUES), which uses an adaptive-size-windows strategy to identify enriched regions (ERs) and cluster them into broad enrichment signals. Tested on 62 ENCODE ChIP-Seq datasets of Ctcf and Nrsf, CLUES performs equally well as MACS2 regarding prioritization of ERs with the TF’s motif. Tested on 165 ENCODE ChIP-Seq datasets of H3K4me3, H3K27me3, and H3K36me3, CLUES performs better than existing algorithms on prioritizing broad enrichment signals implicating cell functions influenced by epigenetic regulatory mechanism in cells. Most importantly, CLUES helps to confirm several novel regulators of mouse ES cell self-renewal and pluripotency through integrated analysis of prioritized broad enrichment signals of H3K4me3, H3K27me3, Nanog and Oct4 with the support of a CRISPR/Cas9 negative selection genetic screen. Conclusions: CLUES holds promise for prioritizing broad enrichment signals from ChIP-Seq data. The download site for CLUES is . [ABSTRACT FROM AUTHOR]
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- 2018
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7. SNORD116 and SNORD115 change expression of multiple genes and modify each other's activity.
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Falaleeva, Marina, Surface, Justin, Shen, Manli, de la Grange, Pierre, and Stamm, Stefan
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GENE expression , *GENETIC code , *NUCLEAR proteins , *RNA , *OBESITY , *SEROTONIN receptors - Abstract
The loss of two gene clusters encoding small nucleolar RNAs, SNORD115 and SNORD116 contribute to Prader–Willi syndrome (PWS), the most common syndromic form of obesity in humans. SNORD115 and SNORD116 are considered to be orphan C/D box snoRNAs (SNORDs) as they do not target rRNAs or snRNAs. SNORD115 exhibits sequence complementarity towards the serotonin receptor 2C , but SNORD116 shows no extended complementarities to known RNAs. To identify molecular targets, we performed genome-wide array analysis after overexpressing SNORD115 and SNORD116 in HEK 293T cells, either alone or together. We found that SNORD116 changes the expression of over 200 genes. SNORD116 mainly changed mRNA expression levels. Surprisingly, we found that SNORD115 changes SNORD116's influence on gene expression. In similar experiments, we compared gene expression in post-mortem hypothalamus between individuals with PWS and aged-matched controls. The synopsis of these experiments resulted in 23 genes whose expression levels were influenced by SNORD116 . Together our results indicate that SNORD115 and SNORD116 influence expression levels of multiple genes and modify each other activity. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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8. Investigation on rapid degradation of sodium dodecyl benzene sulfonate (SDBS) under microwave irradiation in the presence of modified activated carbon powder with ferreous sulfate
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Zhang, Zhaohong, Deng, Yingqiao, Shen, Manli, Han, Wenming, Chen, Zhonglin, Xu, Danping, and Ji, Xiaotong
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CHEMICAL decomposition , *SULFONATES , *MICROWAVES , *ACTIVATED carbon , *POWDER metallurgy , *SULFATES , *ULTRAVIOLET spectroscopy , *INTERMEDIATES (Chemistry) - Abstract
Abstract: The modification of activated carbon powder (ACP) with ferreous sulfate and the degradation of sodium dodecyl benzene sulfonate (SDBS) under microwave irradiation combined with the modified ACP were studied in this work. The research results showed that the catalytic activity of the modified ACP depended on the concentration and acidity of ferreous sulfate solution as well as immersing time. Also, the influences of irradiation time, initial concentration and acidity of SDBS solution and addition amount of modified ACP on the degradation were assessed. UV–vis spectra, FT-IR spectra, ionic chromatography, HPLC and TOC technologies were brought to bear in assessing the modification and the degradation processes. For modified ACP, under given conditions such as 100mg/L SDBS solution, 1.20g/L catalyst dose and pH=6.0, a high degradation ratio (75.5%) was obtained for 25mL solution within 90s microwave irradiation, while it was only 59.59% for unmodified ACP. Furthermore, the degradation ratios could reach 100% by the appropriate increase of addition dose (e.g. 2.80g/L) of modified ACP or the extension of irradiation time (e.g. 230s). Whereas, for unmodified ACP, the corresponding degradation ratios were only 88.71% or 83.54%, respectively. Hence, it can be concluded that the method adopting microwave irradiation combined with the modified ACP reveals many advantages of rapid degradation rate, low cost, no residual intermediates and no secondary pollution in actual application. [Copyright &y& Elsevier]
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- 2009
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9. Correction: Clustering-local-unique-enriched-signals (CLUES) promotes identification of novel regulators of ES cell self-renewal and pluripotency.
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Wu, Chao, Jiao, Yang, Shen, Manli, Pan, Chen, Cheng, Guo, Jia, Danmei, Zhu, Jing, Zhang, Long, Zheng, Min, and Jia, Junling
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AUTOPOIESIS , *PLURIPOTENT stem cells - Published
- 2018
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10. Preparation of N,F-codoped TiO2 nanoparticles by three different methods and comparison of visible-light photocatalytic performances.
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Guo, Qing, Zhang, Zhaohong, Ma, Xiping, Jing, Kui, Shen, Manli, Yu, Ning, Tang, Jianhua, and Dionysiou, Dionysios D.
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WASTEWATER treatment , *TITANIUM dioxide nanoparticles , *PHOTOCATALYSIS , *SOL-gel processes , *NITROGEN , *FLUORINE - Abstract
In this work, three types of N,F-codoped TiO 2 nanoparticles (N,F-TiO 2 ) were synthesized using modified sol-gel methods, and applied in degradation of acid red B (AR B), as a target pollutant in solution. The extents of the degradation of acid red B were investigated to compare the photocatalytic activities of prepared three photocatalysts. The results show that anatase N,F-TiO 2 nanoparticles can be obtained using the three methods with significantly enhanced photocatalytic degradation extents. The degradation rate under visible light alone is very slow, and the N,F-TiO 2 (1) prepared by different adding sequence of nitrogen and fluorine sources, exhibits higher degradation effect. Also, crystallite sizes and dispersion of particles, adding sequence of nitrogen and fluorine sources, doped dose, and treatment temperature have significant effects on the photocatalytic activity. Moreover, more OH radicals are generated under visible light, in the presence of N,F-TiO 2 (1) than those in the presence of N,F-TiO 2 (2) prepared by double sol method, and N,F-TiO 2 (3) prepared by direct method. Therefore, this work suggests that preparation of N,F-TiO 2 (1) nanoparticles using hydrofluoric acid as fluorine sources and ammonia water as nitrogen sources is promising, and N,F-TiO 2 (1) is a promising photocatalyst in applications dealing with treatment of dye wastewater using solar light. [ABSTRACT FROM AUTHOR]
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- 2017
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11. Microwave-induced carbon nanotubes catalytic degradation of organic pollutants in aqueous solution.
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Chen, Jing, Xue, Shuang, Song, Youtao, Shen, Manli, Zhang, Zhaohong, Yuan, Tianxin, Tian, Fangyuan, and Dionysiou, Dionysios D.
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MICROWAVES , *CARBON nanotubes , *CATALYTIC activity , *CHEMICAL decomposition , *POLLUTANTS , *AQUEOUS solutions , *IRRADIATION - Abstract
In this study, a new catalytic degradation technology using microwave induced carbon nanotubes (MW/CNTs) was proposed and applied in the treatment of organic pollutants in aqueous solution. The catalytic activity of three CNTs of 10–20 nm, 20–40 nm, and 40–60 nm diameters were compared. The results showed that organic pollutants such as methyl orange (MO), methyl parathion (MP), sodium dodecyl benzene sulfonate (SDBS), bisphenol A (BPA), and methylene blue (MB) in aqueous solution could be degraded effectively and rapidly in MW/CNTs system. CNTs with diameter of 10–20 nm exhibited the highest catalytic activity of the three CNTs under MW irradiation. Further, complete degradation was obtained using 10–20 nm CNTs within 7.0 min irradiation when 25 mL MO solution (25 mg/L), 1.2 g/L catalyst dose, 450 W, 2450 MHz, and pH = 6.0 were applied. The rate constants (k) for the degradation of SDBS, MB, MP, MO and BPA using 10–20 nm CNTs/MW system were 0.726, 0.679, 0.463, 0.334 and 0.168 min −1 , respectively. Therefore, this technology may have potential application for the treatment of targeted organic pollutants in wastewaters. [ABSTRACT FROM AUTHOR]
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- 2016
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12. Spectroscopic study on interaction between three cationic surfactants with different alkyl chain lengths and DNA.
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Guo, Lili, Zhang, Zhaohong, Qiao, Heng, Liu, Miao, Shen, Manli, Yuan, Tianxin, Chen, Jing, and Dionysiou, Dionysios D.
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MOLECULAR interactions , *CATIONIC surfactants , *CHAIN length (Chemistry) , *DNA analysis , *AMMONIUM bromide , *FLUORESCENCE spectroscopy - Abstract
In this study, the interaction between cationic surfactants with different alkyl chain lengths, such as hexyltrimethyl ammonium bromide (HTAB), dodecyltrimethyl ammonium bromide (DTAB) and cetyltrimethyl ammonium bromide (CTAB), and DNA was investigated by UV–vis spectroscopy, fluorescence spectroscopy and viscosity techniques. The results showed that these three cationic surfactants with different hydrocarbon chain lengths could all interact with DNA. Their binding modes were estimated and their interaction strength was compared. In addition, the effects of the surfactant, NaCl and phosphate ion concentrations on the interaction were reviewed. It is wished that this work would provide some valuable references to investigate the influence of cationic surfactants with different alkyl chain lengths on DNA. [ABSTRACT FROM AUTHOR]
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- 2015
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13. Valproic Acid Causes Proteasomal Degradation of DICER and Influences miRNA Expression.
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Zhang, Zhaiyi, Convertini, Paolo, Shen, Manli, Xu, Xiu, Lemoine, Frédéric, de la Grange, Pierre, Andres, Douglas A., and Stamm, Stefan
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MICRORNA , *GENE expression , *RIBONUCLEASES , *TREATMENT of epilepsy , *THERAPEUTICS , *BIPOLAR disorder , *PROTEASOME inhibitors , *VALPROIC acid - Abstract
Valproic acid (VPA) is a commonly used drug to treat epilepsy and bipolar disorders. Known properties of VPA are inhibitions of histone deacetylases and activation of extracellular signal regulated kinases (ERK), which cannot fully explain VPA’s clinical features. We found that VPA induces the proteasomal degradation of DICER, a key protein in the generation of micro RNAs. Unexpectedly, the concentration of several micro RNAs increases after VPA treatment, which is caused by the upregulation of their hosting genes prior to DICER degradation. The data suggest that a loss of DICER protein and changes in micro RNA concentration contributes to the clinical properties of VPA. VPA can be used experimentally to down regulate DICER protein levels, which likely reflects a natural regulation of DICER. [ABSTRACT FROM AUTHOR]
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- 2013
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14. Function of alternative splicing
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Kelemen, Olga, Convertini, Paolo, Zhang, Zhaiyi, Wen, Yuan, Shen, Manli, Falaleeva, Marina, and Stamm, Stefan
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ALTERNATIVE RNA splicing , *RNA polymerases , *MESSENGER RNA , *CELL proliferation , *GENETIC regulation , *FUNCTION (Biology) - Abstract
Abstract: Almost all polymerase II transcripts undergo alternative pre-mRNA splicing. Here, we review the functions of alternative splicing events that have been experimentally determined. The overall function of alternative splicing is to increase the diversity of mRNAs expressed from the genome. Alternative splicing changes proteins encoded by mRNAs, which has profound functional effects. Experimental analysis of these protein isoforms showed that alternative splicing regulates binding between proteins, between proteins and nucleic acids as well as between proteins and membranes. Alternative splicing regulates the localization of proteins, their enzymatic properties and their interaction with ligands. In most cases, changes caused by individual splicing isoforms are small. However, cells typically coordinate numerous changes in ‘splicing programs’, which can have strong effects on cell proliferation, cell survival and properties of the nervous system. Due to its widespread usage and molecular versatility, alternative splicing emerges as a central element in gene regulation that interferes with almost every biological function analyzed. [Copyright &y& Elsevier]
- Published
- 2013
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15. Steered molecular dynamics simulation of the binding of the β2 and β3 regions in domain-swapped human cystatin C dimer.
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He, Jianwei, Xu, Linan, Zhang, Shuo, Guan, Jing, Shen, Manli, Li, Hui, and Song, Youtao
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CYSTATINS , *PROTEIN binding , *MOLECULAR dynamics , *SIMULATION methods & models , *CRYSTAL structure , *DIMERS , *ELECTROSTATICS - Abstract
The crystal structure of the human cystatin C (hCC) dimer revealed that a stable twofold-symmetric dimer was formed via 3D domain swapping. Domain swapping with the need for near-complete unfolding has been proposed as a possible route for amyloid fibril initiation. Thus, the interesting interactions that occur between the two molecules may be important for the further aggregation of the protein. In this work, we performed steered molecular dynamics (SMD) simulations to investigate the dissociation of the β2 and β3 strands in the hCC dimer. The energy changes observed during the SMD simulations showed that electrostatic interactions were the dominant interactions involved in stabilizing the two parts of the dimer during the early stages of SMD simulation, whereas van der Waals (VDW) interactions and electrostatic interactions were equally matched during the latter stages. Furthermore, our data indicated that the two parts of the dimer are stabilized by intermolecular hydrogen bonds among the residues Arg51 (β2), Gln48 (β2), Asp65 (β3), and Glu67 (β3), salt bridges among the residues Arg53 (β2), Arg51 (β2), and Asp65 (β3), and VDW interactions among the residues Gln48 (β2), Arg51 (β2), Glu67 (β3), Asp65 (β3), Phe63 (β3), and Asn61 (β3). The residues Gln48 (β2), Arg51 (β2), Asp65 (β3) and Glu67 (β3) appear to be crucial, as they play important roles in both electrostatic and VDW interactions. Thus, the present study determined the key residues involved in the stabilization of the domain-swapped dimer structure, and also provided molecular-level insights into the dissociation process of the hCC dimer. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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16. Improving methane productivity of waste activated sludge by ultrasound and alkali pretreatment in microbial electrolysis cell and anaerobic digestion coupled system.
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Bao, Hongxu, Yang, Hua, Zhang, Hao, Liu, Yichen, Su, Hongzhi, and Shen, Manli
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ANAEROBIC digestion , *METHANE as fuel , *MICROBIAL cells , *METHANE , *ALKALIES , *BACTERIAL cells - Abstract
In order to enhance the productivity of methane from the waste activated sludge (WAS), a coupled system of microbial electrolysis cell (MEC) and anaerobic digestion (AD) was proposed. In this study, alkali, ultrasound-alkali, high-temperature coupled microaeration (TM) were applied as pretreatment methods to disintegrate the WAS flocs and break bacterial cell. After ultrasound-alkali pretreatment, the maximum accumulated concentration of VFAs and SCOD increased by 6.4 and 13.8 times compared with the initial concentration, which were 2.8 and 2.6 times of alkali pretreatment, and 2.1 and 2.1 times of TM pretreatment. Then, the pretreated sludge was transferred into MEC-AD coupled reactors and control group of AD reactors. The results showed that, methane production rate was enhanced to 0.15 m3 CH 4 /m3 reactor/d in the coupled reactors, which was improved by 3 times compared with control AD (0.05 m3 CH 4 /m3 reactor/d). The methane yield of MEC-AD coupled reactors achieved 808 ± 8 mL, which were increased by 97.0% ± 1.85% compared to control AD (410 mL). Using MEC can promote the rate of organics degradation and methane yield. The MEC-AD coupled system realized a good performance on the treatment of WAS and improved the efficiency of methane production. Image 1 • A feasibility of combing the ultrasound and alkali pretreatment enhanced organics release. • A significant enhancement was obtained with the coupling reactor. • The organic degradation has been enhanced with aid of MEC. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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