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2 results on '"Ramírez, Heidi Giselle Rodríguez"'

1. Using a quick dye for the diagnosis of microsporidiosis.

Author

Tovar, Luis Edgar Rodríguez, Ramírez, Heidi Giselle Rodríguez, Nava, Yazel Valdez, Garza, Alicia Magdalena Nevárez, and Romero, Rafael Ramírez

Subjects
*MICROSPORIDIOSIS, *DYES & dyeing, *PARAFFIN wax, *TOLUIDINE blue, *COST effectiveness, *DIAGNOSIS, RABBIT diseases
Abstract

Cystic-like structures similar to xenomas containing ovoid or piriform organisms were observed in brain and kidney of a one-month-old rabbit. Different dyes such as H&E, PAS, toluidine blue and trichrome blue were tested. Trichrome blue yielded the best results in terms of specificity, resolution and confirmation of microsporidia spores. This dye is easily adaptable to paraffin-embedded samples by not requiring additional equipment; it is rapid, cheap and could be used as a confirmatory technique for other microsporidiosis. [ABSTRACT FROM AUTHOR]

Published
2011

2. Empleo de una tinción rápida para el diagnóstico de microsporidiosis.

Author

Tovar, Luis Edgar Rodríguez, Ramírez, Heidi Giselle Rodríguez, Nava, Yazel Valdez, Garza, Alicia Magdalena Nevárez, and Romero, Rafael Ramírez

Subjects
*MICROSPORIDIOSIS, *DYES & dyeing, *TOLUIDINE blue, *PARAFFIN wax, *COST effectiveness, *DIAGNOSIS, RABBIT diseases
Abstract

Se observaron estructuras quísticas similares a xenomas con organismos ovoides o piriformes en encéfalo y riñón de un conejo de un mes de edad. Se probaron diferentes tinciones, como HE, PAS, azul de toluidina y azul de tricromo. El azul de tricromo proporcionó los mejores resultados en cuanto a especificidad, resolución y confirmación de esporas de microsporidios. Este colorante es fácilmente adaptable a cortes desparafinados por no requerir de equipo adicional; es rápido, económico y podría utilizarse como técnica confirmatoria para otras microsporidiosis. [ABSTRACT FROM AUTHOR]

Published
2011

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