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Aims: Microbial family identification of 16S rDNA sequences by applying a strategy based on algorithms for data compression. Methods and Results: Perl scripts were developed to analyse similarities in microbial sequences, based on a gzip data compression technique. For each bacterial family ( n = 196) a 16S rRNA reference file was constructed to compare new queries looking at compression performance. An online user-friendly bioinformatics tool was built up to attribute a bacterial family to a 16S rRNA sequence. It was successfully applied to recognize different bacterial families, including Legionellaceae, Bacillaceae, Enterobacteriaceae, Acetobacteriaceae and Rhizobiaceae. The percentage of positive identifications is higher than 95% for fragments over 450 bp. Conclusions: A new bioinformatics approach has been developed to assign a taxonomic classification to a 16SrDNA sequence. An online tool provides quick and easy sequence attribution. The general principle can be applied to other genes of taxonomic interest. Significance and Impact of the Study: Availability of simple bioinformatics tools can support the development of molecular-based analysis and classification of bacteria, especially for environmental or uncultured strains. [ABSTRACT FROM AUTHOR]

Since the beginning of the COVID-19 pandemic, a decrease in physical activity (PA) related to home confinement has been reported worldwide. However, some individuals were able to engage in physical activities at home. Thus, in a perspective of public health, it may be useful to analyse the available evidence regarding PA adopted during home restrictions, in order to identify possible strategies to help people stay active even during emergency situations. The aim of this review was to analyse how healthy individuals spontaneously exercised at home in the course of the pandemic, in order to detect possible factors associated with this behaviour. A systematic review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The protocol was registered in PROSPERO, an international prospective register of systematic reviews, with the registration number CRD42023394673. A literature search was conducted in PubMed, Scopus, and Web of Science. Cross-sectional studies published in English from the inception of each database to February 06th 2023 and focused on healthy individuals practicing spontaneous PA/exercise at home during the pandemic were considered eligible. The quality assessment was performed using the adapted Newcastle–Ottawa Quality Assessment Scale. Bibliographic information, sample size, study paricipant/population with age, gender, ethnicity, socioeconomic status, education, smart workers or not, anthropometric parameters, characteristics of at home exercises, athletic status and sedentariness, associated health-related effects, and main findings were synthetised. From 504 articles, 19 were included. Notwithstanding the differences in the studies examined, the majority of them reported that previous PA level was associated with exercise in such challenging conditions. Furthermore, technologies aimed at supporting exercise were shown to be a useful resource. Being habitually active and using digital supports may be associated with a positive attitude towards exercise at home during isolation. This suggests that in emergency situations, exercise should be promoted, also through digital media, especially among those groups who are usually less engaged in PA. Further analyses of longitudinal studies are needed to confirm these findings. [ABSTRACT FROM AUTHOR]

Abstract: The identification of rapid methods for the surveillance of recreational waters and aquatic environments is necessary to provide adequate levels of health safety and quality standards. Molecular techniques have been proposed in recent years as a valuable alternative to traditional microbiological cultural methods, having numerous advantages (speed, specificity and sensibility). We have previously described a method for the detection of Staphylococcus aureus in recreational waters by a molecular enrichment approach, based on a two steps PCR performed on 23S rDNA. The approach has been now expanded to other microbiological indicators, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli. This methodological extension to other prokaryotic genomes has required a partial primer re-design and protocol optimization, in order to improve the assay. The usage of native not recombinant Taq-polymerases for detection of E. coli eliminated the background probably due to traces of the bacterial genome in many commercially available reagents. The obtained results clearly underline the feasibility of a fast and integrated molecular approach to quantify the standard biological indicators for assessment of safety and quality in recreational waters. [Copyright &y& Elsevier]

Body piercing is defined as the penetration of jewellery into openings made in different body areas. In the last decades, it has become increasingly common in the general population. The aim is to analyse the available literature about complications from body piercing for contributing to raise the awareness towards this issue and to plan and perform appropriate prevention interventions. This is a systematic review. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines were used to perform this systematic review; the protocol was registered with PROSPERO [CRD42020177972]. PubMed, Scopus and Web of Science were searched from database inception to April 2020, and articles in English language reporting a proven association between piercings and health risks were included, and their quality were assessed by adequate quality assessment tools. A total of 4748 studies were found, and after duplicates removal and screening, 84 articles were included. Studies dealing with microbiological effects reported these complications both locally and at distant sites as a result of the spreading of the primary site infection: mastitis, endocarditis, glomerulonephritis, cephalic tetanus, viral hepatitis, HIV, cerebellar brain abscess and toxic shock syndrome. In addition, bleeding, gingival recession, dental injuries, contact dermatitis, granulomatous dermatitis, keloid, fibroma and basal cell carcinoma were reported. Health authorities should promote educational campaigns to spread the knowledge on health risks related to piercings. Besides, piercers are often not adequately aware of all the possible adverse effects due to piercings because their professional training differs among countries. It is therefore advisable to provide periodical upgrade of their education in this field. [ABSTRACT FROM AUTHOR]

The identification of vaginal fluids is crucial in forensic science. Several molecular protocols based on PCR amplification of mfDNA (microflora DNA) specific for vaginal bacteria are now available. Unfortunately mfDNA extraction and PCR reactions require manual optimization of several steps. The aim of present study was the verification of a partial automatization of vaginal fluids identification through two instruments widely diffused in forensic laboratories: EZ1 Advanced robot and Rotor Gene Q 5Plex HRM. Moreover, taking advantage of 5-plex thermocycler technology, the ForFluid kit performances were improved by expanding the mfDNA characterization panel with a new bacterial target for vaginal fluids and with an internal positive control (IPC) to monitor PCR inhibition. Results underlined the feasibility of a semi-automated extraction of mfDNA using a BioRobot and demonstrated the analytical improvements of the kit. [ABSTRACT FROM AUTHOR]

The identification of the source of a specific soil sample is a crucial step in forensic investigations. Rapid advances in next generation sequencing (NGS) technology and the strong reduction of the cost of sequencing have recently opened new perspectives. In the present work a metabarcoding approach has been successfully applied to forensic and environmental soil samples, allowing the accurate and sensitive analysis of microflora (mfDNA), plants, metazoa, and protozoa DNA. The identification of the biological component by DNA metabarcoding is a strong element for the discrimination of samples geologically very similar but coming for distinct environments. [ABSTRACT FROM AUTHOR]

Abstract: The swimming pool legislation is extremely various and uneven. This diversity is related to the different approaches of individual health authorities, attributable to the different social, economic and cultural conditions of each country. In Italy the regulation about sanitary aspects for the construction, maintenance and control of swimming pools refers to the Agreement of January 16, 2003 among the Ministry of Health, the Regions and the Autonomous Provinces of Trento and Bolzano. The Agreement is not a jurisprudential act, but has the character of a document approved by constitutional organs of the same level, aimed to promote the harmonization of respective legislations or the attainment of unitary positions. The controls and the related samples will be collected by Local Health Authority in compliance with criteria established by each region on the basis of specific control plans and supervision and in a manner and frequency that take into account the type of existing facilities inside the specific territorial areas, with particular attention to the critical points highlighted in the protocols of management and the self-prepared safety plans. In this regard, the working Group of Movement Sciences for the Health (GMSH) of the Italian Society of Preventive Medicine and Hygiene (SItI), proposes a checklist to complete during inspections routinely conducted by Local Health Authorities in swimming facilities. The aim is to have a single tool that can homogenize supervisory activities, procedures and types of controls in different realities, both locally and nationally. [Copyright &y& Elsevier]

The analysis of human genetic variability can lead to the comprehension of medical issues and to the development of personalized therapeutic protocols. Single nucleotide polymorphisms, are the most common type of human genetic variation and have been associated to disease development and phenotype forecasting. The recent technologies for DNA sequencing and bioinformatic analysis are now giving the opportunity to develop new diagnostic and prevention approaches also through health promotion protocols. The genetic data management is at the same time underlining technical limitations and old ethical issues. [Copyright &y& Elsevier]

Abstract: Hydrogen sulfide (H2S) is a molecule dissolved in many thermal spring waters at variable concentration. The H2S effects of thermal waters treatments have long been studied, for dermatological and clinical treatments, but its role in recreational waters was never investigated. The use of sulfur spring waters in pools raises concerns related to disinfection by oxidants. The aim of this work is to evaluate the survival rate of microbial species in waters with different titers of H2S. Four selected thermal waters collected from Italian springs, belonging to different chemical categories, have been tested in comparison to Tyrrhenian sea water and natural mineral bottled water. Results show inhibition properties on bacterial proliferation that seem related to H2S concentrations. To further asses this phenomenon H2S was added to thermal and natural mineral waters. The results strongly support a bactericidal role of H2S in thermal spring waters used for recreational purposes. These observations open up new perspectives for a disinfectant role of H2S in pool treatment and management. [Copyright &y& Elsevier]

Bioaerosols associated with wastewater treatment process may represent a health risk for occupationally exposed personnel. To evaluate microbial contamination in plant workers, we compared oral cavity isolates against isolates collected from aerosol surrounding the aeration basin. Typing was performed by metabolic profile and arbitrarily primed-polymerase chain reaction. The latter is more sensitive and rapid than conventional tests. After comparison, isolates from the air samples were not related to those obtained from the exposed workers. This molecular approach can support bioaerosol risk evaluation. [Copyright &y& Elsevier]

Background: In the last decades, body art practices such as tattooing and piercing have known an increasing popularity, mainly in young people. These procedures are associated with infectious and non-infectious health risks. The aim of this multicenter study was to evaluate the knowledge of health risks related to body art procedures among undergraduate students from ten Italian universities. Methods: A cross-sectional study was performed on undergraduate students attending universities in northern, central and southern Italy. An anonymous questionnaire was administered to the participants for collecting their socio-demographic characteristics and for assessing their knowledge about health risks associated with tattoos and piercing. Results: A total of 2985 students (mean age 23.15 3.99, 73.9% females) completed the questionnaire. 775 (25.9%) of them were from northern Italy, 906 (30.3%) from the Centre and 1324 (44.4%) from the South. More than 90% of the sample were aware that tattoo/piercing procedures can cause health problems. However, the mean values of correct answers about the health risks associated to body art practices were low (5.38 2.39 on a total of 11 correct answers for tattooing and 5.93 3.12 on a total of 14 for piercing). Higher knowledge of the health risks related to tattooing was found among those attending university since more than four years (p < 0.001), life science courses (p < 0.001) and residing/living in the university area (p = 0.023). Those attending North/Centre universities (p < 0.001), since more than 4 years (p < 0.001), life science courses (p < 0.001), and those with graduated father (p = 0.013) had better knowledge of the health risks related to piercing. Conclusions: These results show a lack of knowledge about health risks related to body art practices in the sample. Educational interventions on this issue targeted to youth are needed in Italy. Key messages: Undergraduates do not show a good knowledge of health risks related to tattooing and piercing. Educational interventions are needed to raise youth awareness and knowledge of health risks of body art. [ABSTRACT FROM AUTHOR]