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Objective: To explore the effect of abdominal acupuncture combined with artificial cycle on endometrial receptivity after intrauterine adhesion surgery. Method: 40 patients with intrauterine adhesions who were treated in our hospital from January 2018 to August 2018 were randomly divided into experimental group and control group with 20 cases in each group. The control group was treated with artificial cycle, and the experimental group was treated with abdominal acupuncture on this basis. Ultrasound Salle scores were compared between the two groups, and the overall effect of the two groups was evaluated. Result: Before treatment, the Salle score of the experimental group was (10.43 ± 1.77) points, while that of the control group was (10.38 ± 1.82) points, with no significant difference (P > 0.05); after treatment, the Salle score of the experimental group was (17.7 ± 1.87) points, and that of the control group was (13.05 ± 1.95) points. There was significant difference between the two groups (P < 0.05). The total effective rate was 90.00% (18/20) in the experimental group and 60.00% (12/20) in the control group. There was a significant difference between the two groups (P < 0.05). Conclusions: TCM abdominal acupuncture combined with artificial cycle can effectively improve endometrial receptivity after uterine adhesion surgery; thus, it is worthy of clinical application. [ABSTRACT FROM AUTHOR]

The replication of coronaviruses, including severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory syndrome coronavirus (MERSCoV), and the recently emerged severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is closely associated with the endoplasmic reticulum (ER) of infected cells. The unfolded protein response (UPR), which is mediated by ER stress (ERS), is a typical outcome in coronavirus-infected cells and is closely associated with the characteristics of coronaviruses. However, the interaction between virus-induced ERS and coronavirus replication is poorly understood. Here, we demonstrate that infection with the betacoronavirus porcine hemagglutinating encephalomyelitis virus (PHEV) induced ERS and triggered all three branches of the UPR signaling pathway both in vitro and in vivo. In addition, ERS suppressed PHEV replication in mouse neuro-2a (N2a) cells primarily by activating the protein kinase R-like ER kinase (PERK)-eukaryotic initiation factor 2α (eIF2α) axis of the UPR. Moreover, another eIF2a phosphorylation kinase, interferon (IFN)-induced double-stranded RNA-dependent protein kinase (PKR), was also activated and acted cooperatively with PERK to decrease PHEV replication. Furthermore, we demonstrate that the PERK/PKR-eIF2a pathways negatively regulated PHEV replication by attenuating global protein translation. Phosphorylated eIF2a also promoted the formation of stress granules (SGs), which in turn repressed PHEV replication. In summary, our study presents a vital aspect of the host innate response to invading pathogens and reveals attractive host targets (e.g., PERK, PKR, and eIF2a) for antiviral drugs. [ABSTRACT FROM AUTHOR]