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This study is aimed to (1) develop an augmented reality-based immune system module for the eleven grade students of high school to be a proper learning media for them; (2) Identify the impact of using the augmented reality-based immune system module on the eleven grade students' learning performance. This study is classified as Research and Development study using ADDIE (Analysis, Design, Development, Implementation, Evaluation) model but limited only until the development stage. The subject of this study were two materials experts, two media experts, two biology teachers, and the students of XI MIPA Public Senior High School 1 Mejobo Kudus. The data were collected using assessment instruments of materials experts, media experts, biology teachers, and students and also pretest and post-test question sheets. The results of the assessments were analyzed qualitatively and quantitatively using SPPS. The results show that (1) the augmented reality-based immune system module is stated as a proper media to be used in the teaching and learning process. It was based on the assessments from the materials experts with the average value of 48.5 categorized as good, the media experts with an average value of 43.5 categorized as good, and from the biology teachers with the average value of 46 categorized as good; (2) the Augmented reality-based immune system module has an impact on improving the students' learning performance on the topic of the immune system. [ABSTRACT FROM AUTHOR]