1. Patterning of AntibodiesUsing Flexographic Printing.
- Author
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Phillips, Christopher O., Govindarajan, Sridhar, Hamblyn, Simon M., Conlan, R. Steven, Gethin, David T., and Claypole, Tim C.
- Subjects
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IMMUNOGLOBULINS , *PLASTIC films , *FLEXOGRAPHY , *POLYVINYL alcohol , *ADHESION , *SURFACE coatings , *NITROCELLULOSE , *BIOMATERIALS , *BICARBONATE ions , *ENZYMES - Abstract
Antibodies were patterned onto flexible plastic filmsusing theflexographic printing process. An ink formulation was developed usinghigh molecular weight polyvinyl alcohol in carbonateâbicarbonatebuffer. In order to aid both antibody adhesion and the quality ofdefinition in the printed features, a nitrocellulose coating was developedthat was capable of being discretely patterned, thus increasing thesignal-to-noise ratio of an antibody array. Printing antibody featuressuch as dots, squares, text, and fine lines were reproduced effectively.Furthermore, this process could be easily adapted for printing ofother biological materials, including, but not limited to, enzymes,DNA, proteins, aptamers, and cells. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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