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Summary: Multiple shoots were induced from leaf explants of Lycopersicon esculentum cultivar MicroTom, within 20–25d, on MS medium supplemented with 8.9μM benzylaminopurine (BAP)+1.14μM indole-3-acetic acid (IAA). For rooting, elongated microshoots were excised and transferred onto MS medium supplemented with 4.9μM indole-3-butyric acid (IBA). Well-developed roots and flower raceme were obtained on d 7 and 13, respectively, upon transfer of the microshoots onto rooting medium. The flowers self-fertilized in vitro and produced mature fruits in additional 15–17d of culture. [Copyright &y& Elsevier]