1. A simple method for long-term storage of Acanthamoeba species.
- Author
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Diana Axelsson-Olsson, Patrik Ellström, and Jonas Waldenström
- Subjects
- *
ACANTHAMOEBA , *MICROBIAL cryopreservation , *AMOEBIDA , *SURVIVAL analysis (Biometry) , *PARASITOLOGICAL research - Abstract
Abstract We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone–yeast extract–glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27°C, and does not require co-culture with bacteria or any special equipment. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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