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1. Flow cytometric enumeration of bacterial in the coral surface mucus layer.

Author

Bettarel, Yvan, Thanh, Mai Chi, Patrice, Got, Antoinette, Adingra, Nadège, Kouadio-Ngbesso, Bui, Van Ngoc, and Thierry, Bouvier

Subjects
*FLOW cytometry, *FLUORESCENT dyes, *SCLERACTINIA, *CORALS, *MUCUS
Abstract

The direct counts of bacteria inhabiting coral mucus were performed by flow cytometry testing four fluorescent dyes (SYBR®Green I, HCS, TOPRO®3, SYTO®62) with three different scleractinian species. Results obtained with SYTO62 were the most reliable based on the comparison with standardized epifluorescence counts and the resolution of cytograms. [ABSTRACT FROM AUTHOR]

Published
2016
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