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Gels combined with honey might generate new possibilities of textures in food development. This work explores the structural and functional properties of gelatin (5 g/100 g), pectin (1 g/100 g), and carrageenan (1 g/100 g) gels with different content of honey (0–50 g/100 g). Honey decreased the transparency of gels and made them more yellow‐greenish; all of them were firm and uniform, especially at the highest honey content. The water holding capacity increased (63.30–97.90 g/100 g) and moisture content, water activity (0.987–0.884) and syneresis (36.03–1.30 g/100 g) decreased with the addition of honey. This ingredient modified mainly the textural parameters of gelatin (Hardness: 0.82–1.35 N) and carrageenan gels (Hardness: 2.46–2.81 N), whereas only the adhesiveness and the liquid like‐behavior were increased in the pectin gels. Honey increased the solid behavior of gelatin gels (G': 54.64–173.37 Pa) but did not modify the rheological parameters of the carrageenan ones. Honey also had a smoothing effect on the microstructure of gels as observed in the scanning electron microscopy micrographs. This effect was also confirmed by the results of the gray level co‐occurrence matrix and fractal model's analysis (fractal dimension: 1.797–1.527; lacunarity: 1.687–0.322). The principal component and cluster analysis classified samples by the hydrocolloid used, except the gelatin gel with the highest content of honey, which was differentiated as a separate group. Honey modified the texture, rheology, and microstructure of gels indicating that it is possible to generate new products to be used in other food matrices as texturizers. [ABSTRACT FROM AUTHOR]

Simple Summary: Cardiovascular diseases represent the leading cause of mortality and morbidity worldwide, and age is an important contributing factor. Preclinical models are crucial for exploring and understanding the physiology of the heart and age-related changes, as well as cardiac pathological aspects. In line with this, the Octodon degus (O. degus) is a diurnal rodent that has been claimed as an interesting model for aging research. However, several aspects of this experimental model are not fully characterized yet. In this work, we evaluated the cardiac electric activity by electrocardiogram recording in the aging process, considering differences between males and females. We provide the normal ranges for the heart rate, duration and voltage of the ECG waves and intervals, as well as electrical axis deviation. Our results demonstrate that both age and sex influence the cardiac function and the incidence of arrhythmias. Therefore, this research indicates that this rodent model could be useful for cardiovascular research, including impacts of aging and biological sex. Cardiovascular diseases represent the leading cause of mortality and morbidity worldwide, and age is an important risk factor. Preclinical models provide supportive evidence toward age-related cardiac changes, as well as allow for the study of pathological aspects of the disease. In the present work, we evaluated the electrocardiogram (ECG) recording in the O. degus during the aging process in both females and males. Taking into account the age and sex, our study provides the normal ranges for the heart rate, duration and voltage of the ECG waves and intervals, as well as electrical axis deviation. We found that the QRS complex duration and QTc significantly increased with age, whereas the heart rate significantly decreased. On the other hand, the P wave, PR and QTc segments durations, S wave voltage and electrical axis were found to be significantly different between males and females. The heart rhythm was also altered in aged animals, resulting in an increased incidence of arrhythmias, especially in males. Based on these results, we suggest that this rodent model could be useful for cardiovascular research, including impacts of aging and biological sex. [ABSTRACT FROM AUTHOR]

The role of women human rights defenders in Colombia is fundamental. The conditions of socioeconomic inequality, gender-based inequalities and discrimination, and the armed conflict have been complex scenarios in which women's leadership in the defence of human rights and peace have played a leading role. The creation of diverse collective processes and social networks at the local and national levels have been crucial strategies in the work of women defenders, their advocacy, and their forms of resistance. Their impact is undeniable. However, the work of women human rights defenders in Colombia has not been easy and has been frequently threatened. Hundreds of women human rights defenders have left the country as exiles over the past three decades or have been forcibly displaced within the country to protect their lives, many have suffered direct attacks, and some of them have been killed. The forms and impacts of violence against them have differential characteristics with respect to other defenders, which reveal the presence of gender stereotypes. In addition, indigenous, peasant, and lesbian, bisexual and trans women (LBT women) defenders face aggravated risks. Paradoxically, their visibility in the defence of peace has had very high costs for them after the signing of the Peace Accord between government and FARC-EP guerrillas in 2016. Women here are at a differential risk not only because they support the Peace Accord implementation in contexts where there are sectors that oppose it, but because they have gone beyond the low-key behaviour that is still expected for women in the political arena in many places of Colombia. Despite all this, they continue to lead the defence of human rights and their political advocacy has led to the creation of specific protection programmes for them by the Colombian state, the implementation of which is currently a challenge. [ABSTRACT FROM AUTHOR]

This document is an editorial published in the journal Frontiers in Insect Science. It discusses the current status of honey bee genetic and breeding programs, highlighting the progress made and future perspectives. The editorial emphasizes the importance of honey bees in food production and biodiversity, as well as the challenges they face due to agriculture intensification, climate change, and diseases. The article also mentions the value of honey bee products and the interest of geneticists and breeders in their genetic and behavioral attributes. The editorial concludes by highlighting the potential benefits of incorporating genetic knowledge into honey bee breeding programs, particularly in relation to Varroa resistance and the reduction of synthetic acaricides. However, it notes that these advancements are not yet widely applied in commercial breeding programs. [Extracted from the article]

Severe Acute Respiratory Syndrome (SARS) is the serious condition of coronavirus (COVID-19) caused by SARS-COV-2 which causes respiratory infections in humans, and whose transmission is given mainly through the contact. this virus enters into the host cell due to the spike protein (S) interaction with the angiotensin-converting enzyme 2 (ACE2), which is not only present in the respiratory tract but also monocytes, macrophages, endothelial cells, the heart, and gastrointestinal tract. The increase in INF stops viral replication and activates the adaptive immune response. The infection's clinic manifestations often occur in the respiratory tract; however, other systems like the hematopoietic may be affected. Altered cell counts, mainly leukocytes and platelets, are seen on the blood count. Lymphopenia and neutrophilia are associated with severe disease; thrombocytopenia is present in a heterogeneous way in the infection. Among the disease's complications are the Disseminated Intravascular Coagulation (DIC) that results when monocytes and endothelial cells are activated because of the release of cytokines, causing endothelial damage, with the synthesis of the tissue factor, tissue factor discharge, platelet activation, and the von Willebrand factor release, generating a hyperfibrinolytic condition especially in the infection's late-stage. Laboratory tests such as D-dimer (D-D), Fibrinogen Degradation Products (FDP), Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT) among others, are essential to the diagnosis, monitoring, and prognosis of the disease. [ABSTRACT FROM AUTHOR]

Abstract Long-time low-temperature (LTLT) cooking of meat is known to produce a tender product. The current work tested the hypothesis that LTLT cooking for periods of up to 24 h at 60 °C reduces the contribution of intramuscular connective tissue to cooked meat toughness. Tensile tests on perimysium excised after cooking showed that its strength diminished with cooking time, although not as markedly as the Warner-Bratzler peak force measure of toughness. A gradually increasing susceptibility to trypsin digestion with increasing heating time demonstrated that there was a slow and gradual increase in the proportion of denatured collagen in the perimysium. Differential scanning calorimetry on perimysium excised after cooking showed an endothermic peak representing the denaturation of the collagen not already denatured on cooking. With increasing cooking time, the energy per milligram of collagen necessary to denature this remaining fraction increased. These results support the hypothesis that there is both an easily destabilized and more resistant fractions of the collagen in intramuscular connective tissue. Highlights • Tensile tests show perimysium in beef semitendinosus muscle is weakened by long-time low-temperature cooking. • Warner-Bratzler shear force measures of toughness partly reflect changes in connective tissue strength. • Denaturation of perimysial collagen at 60 °C is only partially complete after 24 h. • Energy to denature remaining native perimysial collagen increases with time at 60 °C [ABSTRACT FROM AUTHOR]

Objectives: To compare the information provided by the websites of several Colombian and foreign universities, in reference to their strategic platform on training, professional and occupational profiles of the clinical specialist in periodontics. Materials and Methods: Comparative documentary research by intentionally reviewing the websites among six universities, three at a national level (Colombia): CES University, Pontificia Universidad Javeriana (PUJ), also private institutions and one public institution: National University of Colombia (UN). At an international level, universities recognized by different rankings and that had periodontics programs as postgraduate training: University of Chile, University of Sao Paulo, and University of Michigan in Ann Arbor; the information was condensed into a matrix with pre-structured and emerging categories. Results: All institutions declare institution's mission and a program mission; some of them do not declare the three profiles consulted. Regarding the mission of the periodontics programs, two national universities and one international university do not report it. When the training objectives are required, the documentation refers to the object of study and the fields of intervention. The areas of knowledge are clear, however, the techniques, methods and procedures during training are poorly informed. The competency category is the least enunciate. Conclusions: Comparative research allows the interested parties to be informed on little-explored topics, with the aim of establishing similarities or differences and relationships that could guide international academic mobility. [ABSTRACT FROM AUTHOR]

The tomato (Solanum lycopersicum L.) is the most economically important vegetable worldwide and one of the most consumed vegetable in the world. It's wild relatives are native of various habitats ranging from Ecuador, Peru, and Chile and have been employed to generate varieties adapted to specific biotic and abiotic factors worldwide. In order to evaluate the morphological and genetic variation in the germplasm collection at the National University of Loja (UNL) in Ecuador, four wild species were selected: Solanum pimpinellifolium, Solanum neorickii, Solanum habrochaites, Solanum lycopersicum var. cerasiforme. With the morphological variables (20 quantitative, 20 qualitative) phenotypic differences in vegetative components and related to flower and fruit were detected. Only S. habrochaites was differentiated based on these variables. The diversity and genetic structure of the species were evaluated with 17 microsatellite loci. In spite of none of the variability indexes showed statistically significant differences due to the large variance presented, the species S. neorickii exhibited the lowest genetic variability values. The individual genetic distances, the number of groups genetically structured and the genetic differentiation (FST) were congruent and revealed four groups corresponding to each species tested. [ABSTRACT FROM AUTHOR]

In Colombia even exist telecommunication public enterprises, which compete with global companies that arrived to the country as a consequence of privatization and liberalization policies, applied more than two decades ago. The new operation rules give clear advantages to private actors, who have gotten dominance, and make the state companies to get abilities to be able to adjust themselves to new technological and institutional conditions. etb and une, public companies chosen as study object, have taken in a competitive behavior, at the same time that they continue making their social function; this motivates them to get better management practices, in order to give services, nowadays nationwide. But the great investments that demand the permanent technology evolution, force to make public-private alliances, by capitalization and fusion methods, which put the continuity of their roll as tools of ict public policy at risk. [ABSTRACT FROM AUTHOR]

The repercussions of the pandemic caused by the SARS-CoV-2 coronavirus over recent years have posed an unprecedented challenge for the whole of society, affecting the well-being of everyone. Among all the variables affected in relation to well-being, Anxiety, Emotional Intelligence, and Effective Personality (Self-Esteem, Academic Self-Realisation, Resolute Self-Efficacy, Social Self-Realisation) have been highlighted. The aim of this study is to assess the evolution of those variables across three temporal phases: pre-pandemic, during the pandemic, and up until the end of the study in April 2022. A study was conducted during these temporal phases with three cohorts from Spanish Universities. The cohorts were formed of people assessed for Anxiety (660 pre-pandemic, 460 during the pandemic, and 311 at the end of the study), Emotional Intelligence (355 pre-pandemic, 91 during the pandemic, 311 at the end of the study), and Effective Personality (708 pre-pandemic, 174 in 2018, 311 at the end of the study). Anxiety was assessed with the State–Trait Anxiety Inventory, Emotional Intelligence with the Trait Meta-Mood Scale and TMMS-24, and Effective Personality with the Cuestionario Personalidad Eficaz-Universidad (the Effective Personality Questionnaire-University). The results showed a rise in the state of anxiety during COVID-19, with a subsequent reduction two years into the pandemic; however, anxiety rates remained higher than before the pandemic. Emotional intelligence increased in the emotional attention factor, but diminished as regards both clarity and regulation. Effective Personality was at lower levels for all factors (Self-Esteem, Academic Self-Realisation, Resolute Self-Efficacy, Social Self-Realisation). The main conclusion was the need for assistance with the dimensions under study, in order to improve the well-being of university students after the serious effects caused by COVID-19. [ABSTRACT FROM AUTHOR]

Recent biological invasions offer 'natural' laboratories to understand the genetics and ecology of adaptation, hybridization, and range limits. One of the most impressive and well-documented biological invasions of the 20th century began in 1957 when Apis mellifera scutellata honey bees swarmed out of managed experimental colonies in Brazil. This newly-imported subspecies, native to southern and eastern Africa, both hybridized with and out-competed previously-introduced European honey bee subspecies. Populations of scutellata-European hybrid honey bees rapidly expanded and spread across much of the Americas in less than 50 years. We use broad geographic sampling and whole genome sequencing of over 300 bees to map the distribution of scutellata ancestry where the northern and southern invasions have presently stalled, forming replicated hybrid zones with European bee populations in California and Argentina. California is much farther from Brazil, yet these hybrid zones occur at very similar latitudes, consistent with the invasion having reached a climate barrier. At these range limits, we observe genome-wide clines for scutellata ancestry, and parallel clines for wing length that span hundreds of kilometers, supporting a smooth transition from climates favoring scutellata-European hybrid bees to climates where they cannot survive winter. We find no large effect loci maintaining exceptionally steep ancestry transitions. Instead, we find most individual loci have concordant ancestry clines across South America, with a build-up of somewhat steeper clines in regions of the genome with low recombination rates, consistent with many loci of small effect contributing to climate-associated fitness trade-offs. Additionally, we find no substantial reductions in genetic diversity associated with rapid expansions nor complete dropout of scutellata ancestry at any individual loci on either continent, which suggests that the competitive fitness advantage of scutellata ancestry at lower latitudes has a polygenic basis and that scutellata-European hybrid bees maintained large population sizes during their invasion. To test for parallel selection across continents, we develop a null model that accounts for drift in ancestry frequencies during the rapid expansion. We identify several peaks within a larger genomic region where selection has pushed scutellata ancestry to high frequency hundreds of kilometers past the present cline centers in both North and South America and that may underlie high-fitness traits driving the invasion. Author summary: Crop pollination around the world relies on native and introduced honey bee populations, which vary in their behaviors and climatic ranges. Scutellata-European hybrid honey bees (also known as 'Africanized' honey bees) have been some of the most ecologically successful; originating in a 1950s experimental breeding program in Brazil, they rapidly came to dominate across most of the Americas. As a recent genetic mixture of multiple imported Apis mellifera subspecies, scutellata-European hybrid honey bees have a patchwork of ancestry across their genomes, which we leverage to identify loci with an excess of scutellata or European ancestry due to selection. We additionally use the natural replication in this invasion to compare outcomes between North and South America (California and Argentina). We identify several genomic regions with exceptionally high scutellata ancestry across continents and that may underlie favored scutellata-European hybrid honey bee traits (e.g. Varroa mite resistance). We find evidence that a climatic barrier has dramatically slowed the invasion at similar latitudes on both continents. At the current range limits, scutellata ancestry decreases over hundreds of kilometers, creating many bee populations with intermediate scutellata ancestry proportions that can be used to map the genetic basis of segregating traits (here, wing length) and call into question the biological basis for binary 'Africanized' vs. European bee classifications. [ABSTRACT FROM AUTHOR]

Objective: To describe the experiences of older adults around forced displacement due to the Colombian armed conflict. Methods: Interpretive-comprehensive study, with a hermeneutical approach; several types of sampling were carried out. The participants were 12 people aged over 60 years, who reported having being displaced and who participated in the SABE Colombia Survey. The data were encoded using the Atlas.ti software. A process of condensation of central analytical, support and emerging categories was made. Results: The displacement generated by the armed conflict has been decisive in the current life conditions of the participants. They know that they are survivors of someone else's violence; there is dislocation, loss of territory, de-anchoring, lack of protection and insecurity. To the stigma of old age, it is added being displaced and being strangers in a place where they don't belong. They live the violent uprooting of their lands and the confusion of their identity; they found themselves in a foreign scene where they were the unusual and the strangers; from receiving threats, they passed to be labeled as 'threatening'. This forced displacement stems from violence, but also from fear, and it marks the trajectory of life for older people who experience a prolonged struggle for survival in often hostile environments, living "permanently" displaced. Conclusion: When there is displacement, older people are not only shed of their land and their home, but also from their cosmos and their vital referents; in addition, it changes their life trajectory and their place in the world. Interventions should be designed based on specific particular and contextual analyses. [ABSTRACT FROM AUTHOR]

Abstract The neural crest (NC) is a multipotent migratory embryonic population that is formed during late gastrulation and gives rise to a wide array of derivatives, including cells from the peripheral nervous system (PNS), the craniofacial bones and cartilages, peripheral glial cells, and melanocyte cells, among others. In this work we analyzed the role of the Hedgehog signaling pathway effector gli2 in Xenopus NC. We provide evidence that the gli2 gene is expressed in the prospective, premigratory and migratory NC. The use of a specific morpholino against gli2 and the pharmacological specific inhibitor GANT61 in different experimental approaches allowed us to determine that gli2 is required for the induction and specification of NC cells as a transcriptional activator. Moreover, gli2 also acts by reducing apoptosis in the NC without affecting its cell proliferation status. We also demonstrated that gli2 is required cell-autonomously for NC migration, and for the formation of NC derivatives such as the craniofacial cartilages, melanocytes and the cranial ganglia. Altogether, our results showed that gli2 is a key transcriptional activator to accomplish the proper specification and development of Xenopus NC cells. [ABSTRACT FROM AUTHOR]

Objective. To determine the prevalence and risk factors for oral high-risk human papillomavirus (HR-HPV) infection in human immunodeficiency virus (HIV)-infected men. Materials and methods. Consecutive male outpatients with HIV-infection were enrolled. Demographic and behavioral risk data were obtained. Anal swabs and oral rinses were tested for HR-HPV DNA. Oral, pharyngeal and video laryngoscopy examinations were performed for detection of lesions. Results. The prevalence of HR-HPV oral infection was 9.3% (subtypes other than HR HPV 16/18 predominated). The prevalence of anal HR-HPV infection was 75.7%. The risk factors for oral infection with HR-HPV were tonsillectomy (OR=13.12) and years from HIV diagnosis (OR=1.17). Conclusions. Tonsillectomy and years from HIV diagnosis were associated with oral HPV infection. No association was found between oral and anal HR-HPV infections. This is the first study reporting the prevalence and risk factors for oral HR-HPV infection in Mexican HIV-infected population. [ABSTRACT FROM AUTHOR]

Current protocols of anthracycline-induced cardiomyopathy in rabbits present with high premature mortality and nephrotoxicity, thus rendering them unsuitable for studies requiring long-term functional evaluation of myocardial function (e.g., stem cell therapy). We compared two previously described protocols to an in-house developed protocol in three groups: Group DOX2 received doxorubicin 2 mg/kg/week (8 weeks); Group DAU3 received daunorubicin 3 mg/kg/week (10 weeks); and Group DAU4 received daunorubicin 4 mg/kg/week (6 weeks). A cohort of rabbits received saline (control). Results of blood tests, cardiac troponin I, echocardiography, and histopathology were analysed. Whilst DOX2 and DAU3 rabbits showed high premature mortality (50% and 33%, resp.), DAU4 rabbits showed 7.6% premature mortality. None of DOX2 rabbits developed overt dilated cardiomyopathy; 66% of DAU3 rabbits developed overt dilated cardiomyopathy and quickly progressed to severe congestive heart failure. Interestingly, 92% of DAU4 rabbits showed overt dilated cardiomyopathy and 67% developed congestive heart failure exhibiting stable disease. DOX2 and DAU3 rabbits showed alterations of renal function, with DAU3 also exhibiting hepatic function compromise. Thus, a shortened protocol of anthracycline-induced cardiomyopathy as in DAU4 group results in high incidence of overt dilated cardiomyopathy, which insidiously progressed to congestive heart failure, associated to reduced systemic compromise and very low premature mortality. [ABSTRACT FROM AUTHOR]

Grooming behavior confers resistance to honey bees against Varroa destructor, being of interest to social immunity studies and breeding programs. The objective of this study was to characterize at the individual level the grooming behavior of mite-resistant (R) and susceptible (S) A. mellifera stocks from Argentina. Assays were performed in experimental arenas by applying two treatments to nurse bees: (1) placing a V. destructor mite on the bee’s thorax and (2) touching the bee with a paintbrush. Grooming reactions were recorded on bees from both stocks at the ages of 6, 10, and 14 days after emergence. R bees exhibited lower time of first response against the mite, performed more cleaning attempts, and used all their legs with a higher probability compared to S bees. The same pattern was evident when younger and older bees from the R stock were compared. The results demonstrate that bee age and genetic origin are critical factors of grooming behavior in honey bees. [ABSTRACT FROM AUTHOR]

Highlights: [•] Physicochemical and sensory parameters of honeys were studied. [•] Differences were found in colour, conductivity and sucrose among different regions. [•] The cluster analysis showed a grouping pattern in relation to the geographic region. [•] The honeys analysed comply with national and international regulations. [Copyright &y& Elsevier]

This study was designed to investigate the persistence of lipodystrophy (LD)-related social distress and isolation in HIV-infected patients in the current era, according to confirmatory dual energy X-ray absorptiometry (DEXA) measurements. Cross-sectional interview data were collected from 168 HIV-positive adult patients taking more than 2 years of antiretroviral therapy (133 cases with LD diagnosed a mean of 7.2 years before; 35 without LD, controls). Mean time of HIV infection was 16.2 years (2.1–27.3), and the mean time of exposure to highly active antiretroviral therapy of 11.7 years (2.1–21.1). The presence and severity of LD, confirmed by DEXA measurements, correlated with social isolation through a validated scale, including avoidance of social relationships, sex, work, or sport activities. In comparison with control patients, social distress was observed for patients having moderate body changes. The significant correlation between LD and social isolation was irrespective of age, CD4+ count, HIV RNA level, AIDS diagnosis, time of HIV infection, anxiety, or depressive symptoms. These results confirm that patient assessment of LD is correlated with whole-body DEXA scan, and they highlight the role of LD as an independent cause of social isolation even after years of the diagnosis. [ABSTRACT FROM PUBLISHER]

Background To determine the incidence and significance of acute kidney injury (AKI) after initiating highly active antiretroviral therapy (HAART). Methods A prospective cohort study of 271 consecutively treated HIV-infected patients, initiating first (75) or sequential HAART (196) from January 2008 to June 2011. AKI was diagnosed according to the Risk, Injury, Failure, Loss of kidney function, End-stage renal disease (RIFLE)/Acute Kidney Injury Network (AKIN) criteria, and the risk of progression to chronic kidney disease (CKD) was evaluated. Results A greater estimated glomerular filtration rate (eGFR) decrease after 1 year was observed for patients initiating a tenofovir disoproxil fumarate (TDF)-based regimen (−6.45 versus +0.98 mL/min/1.73 m2 when compared with patients without TDF; P < 0.01), both in the case of the first (−8.5 versus −2.27; P = 0.04) or successive regimens (−5.3 versus + 1.18 mL/min/1.73 m2; P < 0.01). AKI, as defined, was observed in 10% (28 cases, 6.98 episodes/100 patients-year), mostly stage I (27 cases), in a median time of 6 (3–16.5) months. Four cases (14%), having a worse baseline renal function progressed to CKD, whereas four recovered completely. In the multivariate analysis, AKI was associated with the concomitant use of cotrimoxazole prophylaxis and to low CD4+ count. CKD was diagnosed in 2% (six cases) of patients. Therefore, the overall rate of HAART-associated renal disorders was 11% (30 cases, 7.46 episodes/100 patients-year (95% confidence interval, 6.09–8.83). Conclusions The initiation of a tenofovir-based regimen is followed by a significant decline in eGFR, although it could be misinterpreted by the concomitant use of cotrimoxazole. A substantial proportion of patients develop AKI, but only a minority progress to CKD. Patients initiating HAART and developing AKI should be carefully monitored for progression of renal disease. [ABSTRACT FROM AUTHOR]

Objective  This work aimed to evaluate the factors associated with Streptococcus pneumoniae nasopharyngeal colonization and antimicrobial susceptibility among pediatric outpatients in southwestern Colombia, 2019. Methods  A cross-sectional study was performed using survey-based interviews and the collection of nasopharyngeal-swab specimens for microbiological characterization and antimicrobial susceptibility testing. Logistic regression analyses were performed for factors associated with nasopharyngeal carriage. Results  A total of 452 children under the age of 5 years were examined in which 41.8% carried S. pneumoniae. Higher pneumococcal carriage frequencies were observed among participants aged <2 years and in individuals belonging to indigenous communities, which were lacking established pneumococcal-conjugated vaccine-10 immunization schemes. Additionally, children attending childcare institutions were also highly colonized by pneumococci. S. pneumoniae showed 57.7% nonsusceptibility to benzyl-penicillin (meningitis-cut); 45.5% intermediate-sensitivity to benzyl-penicillin (oral-cut) and 21.7% to cefotaxime; and resistance to erythromycin (40.7%), tetracycline (36.0%), trimethoprim/sulfamethoxazole (24.9%), clindamycin (24.3%), and ceftriaxone (27.0%). Conclusion  The 41.8% of participants carrying S. pneumoniae show a scenario with the presence of multidrug and extensively drug-resistant strains, which constitutes important reservoirs of bacterial transmission by children aged <5 years in Colombia, leading to an onset of pneumococcal diseases. Hence, there is an urgent need to expand conjugate pneumococcal immunization in the community and ensure compliance with established immunization schedules. [ABSTRACT FROM AUTHOR]

Varroa destructor Anderson & Trueman (Acari: Varroidae) is one of the major contributors to the significant losses of western honey bee, Apis mellifera L. (Hymenoptera: Apidae), colonies worldwide. Hygienic behavior, in which individual workers detect, uncap, and remove unhealthy brood, is a type of social immunity that reduces pathogen and parasite loads in the colony. Previous evidence suggests that hygienic worker bees identify diseased brood through olfactory cues. The aims of the present work were (1) to study the hygienic behavior of worker bees toward V. destructor‐infested cells at different stages of brood development, (2) to explore changes in brood volatile profiles associated with the progression of mite infestation, and (3) to analyze the role of specific volatile compounds in triggering the hygienic behavior. Results showed that the removal rate of infested brood changed along its development and the progression of mite reproduction. Two compounds, ethyl hexanoate and α‐pinene, were present in volatile collections from mite‐infested pupae but absent from uninfested pupae. Field bioassays showed that these volatiles are relevant to elicit the hygienic behavior. A third compound, β‐ocimene, was present in infested and uninfested brood but its abundance pattern varied according to the infestation status throughout brood development. Specifically, for uninfested brood, the abundance of β‐ocimene showed a reduction in black‐eyed pupae, whereas for infested brood, its abundance decreased drastically in light‐pink‐eyed pupae and remained constant in black‐eyed pupae. Our results revealed that olfactory signals associated with V. destructor infestation change as the reproductive cycle of V. destructor progresses inside the cell. These changes can be mimicked to some extent by adding specific volatile compounds to the cell to induce hygienic removal. These findings shed light on the chemical basis of hygienic behavior against V. destructor and could facilitate the development of improved hygienic selection tools to breed mite‐resistant honey bee colonies. [ABSTRACT FROM AUTHOR]

A longitudinal study was carried out in order to evaluate Nosema spp. spore counts in two key moments of the year (late autumn and spring) in honeybee colonies from five eco-regions of Argentina. Three hundred and sixty colonies were randomly selected and the infestation level of Nosema spp. and Varroa destructor was assessed in autumn and spring of 2015. In addition, a questionnaire concerning all management practices applied in the colonies was answered by the beekeepers. Generalized linear mixed effect models were performed to determine the relationship between risk factors associated with Nosema spp. spore counts in each eco-region. Data were scanned to identify colonies cluster with a higher probability of achieving high values of Nosema spp. abundance for each sampling period. Spatial distribution of colonies with a higher risk of achieving high Nosema spp. abundance seems to be explained by geographical conditions associated to climatic conditions and some management practices implemented in each eco-region. [ABSTRACT FROM AUTHOR]

Hypertrophic cardiomyopathy (HCM) is characterized by an abnormal increase in myocardial mass that affects cardiac structure and function. HCM is the most common inherited cardiovascular disease in humans (0.2%) and the most common cardiovascular disease in cats (14.7%). Feline HCM phenotype is very similar to the phenotype found in humans, but the time frame for the development of the disease is significantly shorter. Similar therapeutic agents are used in its treatment and it has the same complications, such as heart failure, thromboembolism and sudden cardiac death. In contrast to humans, in whom thousands of genetic variants have been identified, genetic studies in cats have been limited to fragment analysis of two sarcomeric genes identifying two variants in MYBPC3 and one in MYH7. Two of these variants have also been associated with human disease. The high prevalence of the reported variants in non‐affected cats hinders the assumption of their pathogenicity in heterozygotes. An in‐depth review of the literature about genetic studies on feline HCM in comparison with the same disease in humans is presented here. The close similarity in the phenotype and genotype between cats and humans makes the cat an excellent model for the pathophysiological study of the disease and future therapeutic agents. [ABSTRACT FROM AUTHOR]

During a reproductive cycle, not all daughter mites of Varroa destructor mate and thus leave the brood cells as virgins. Here, we show that virgin mites are present within both the phoretic (10%) and reproductive (8%) mite population. Most (n = 29 of n = 33) of these encountered virgins laid unfertilized (= male) eggs, and some (n = 10) mated later on with their own son. These findings were verified by tests with artificially reared virgin mites. Obviously, mating is not a prerequisite for Varroa reproduction. However, due to the small number of reproductive cycles, the contribution of virgins to the Varroa population is regarded as low. This study also confirms conclusively that sex of V. destructor is determined via arrhenotokous parthenogenesis and not—as previously assumed—via pseudo-arrhenotoky. Furthermore, reproductive parameters of naturally invaded and artificially introduced Varroa females were compared, and artificial infestation was reconfirmed as a suitable method. [ABSTRACT FROM AUTHOR]

Varroa destructor, a parasitic mite of the western honey bee, Apis mellifera L., is a serious threat to colonies and beekeeping worldwide. Population genetics studies of the mite have provided information on two mitochondrial haplotypes infecting honey bee colonies, named K and J (after Korea and Japan, respectively, where they were originally identified). On the American continent, the K haplotype is much more prevalent, with the J haplotype only detected in some areas of Brazil. The aims of the present study were to assess the genetic diversity of V. destructor populations in the major beekeeping region of Argentina and to evaluate the presence of heteroplasmy at the nucleotide level. Phoretic mites were collected from managed A. mellifera colonies in ten localities, and four mitochondrial DNA (mtDNA) regions (COXI, ND4, ND4L, and ND5) were analyzed. Based on cytochrome oxidase subunit I (COXI) sequencing, exclusively the K haplotype of V. destructor was detected. Furthermore, two sub-haplotypes (KArg-N1 and KArg-N2) were identified from a variation in ND4 sequences and the frequency of these sub-haplotypes was found to significantly correlate with geographical latitude. The occurrence of site heteroplasmy was also evident for this gene. Therefore, ND4 appears to be a sensitive marker for detecting genetic variability in mite populations. Site heteroplasmy emerges as a phenomenon that could be relatively frequent in V. destructor. [ABSTRACT FROM AUTHOR]

The European honeybee, Apis mellifera L. (Hymenoptera: Apidae), is considered as a main contributor to pollination of important crops and to honey production. Originally, beekeeping in Argentina was performed in an extended area covering the north and central region of the country and involving A. mellifera of European origin. Later, honeybees of African origin entered South America through Brazil and hybridized with European genetic resources, giving rise to Africanized populations that are characterized by a more aggressive behavior among other unfavorable traits. In this study, a genetic characterization of 396 honeybee colonies from the most important apicultural region of Argentina is presented in order to provide an updated description of population structure and genetic diversity of commercial and feral colonies. Diversity was analyzed using mitochondrial (COI‐COII region) and nuclear (eight microsatellites) markers. Three European (M4, C1, C2J) and three African (A1, A4, A30) haplotypes were detected. European haplotypes were mostly found in commercial apiaries, whereas African haplotypes were detected at high frequencies in feral colonies. Microsatellite data were analyzed to estimate population genetic variability at the province level and to evaluate genetic admixture. A high level of hybridization between Africanized and European honeybees was detected with a significant latitudinal cline from north to south. Extensive population admixture resulted in the definition of four clusters that included both feral and commercial colonies and that are explained not only by geographical distribution and degree of Africanization but also by human influence through beekeeping activities. Genetic characterization of 396 Argentinean honeybee colonies provides an updated description of population structure and diversity of commercial and feral colonies. European haplotypes were mostly found in commercial apiaries, whereas African haplotypes were often detected in feral colonies. Microsatellite data indicated a high level of hybridization between Africanized and European honeybees with a cline from north to south. Extensive population admixture resulted in the definition of four clusters, explained by geographical distribution, degree of Africanization, and human influence through apiculturing. [ABSTRACT FROM AUTHOR]

Hygienic behaviour is a complex trait that gives Apis mellifera L. resistance against brood diseases. Variability in the expression of hygienic behaviour is evidenced at the colony-level and is explained by the proportion and propensity of individual worker bees that engage in hygienic activities. We investigated the temporal performance and the dynamics of task-specialisation of individual bees over time, both in selected hygienic (H) and non-hygienic (NH) colonies. Then we evaluated the impact of these behavioural aspects on the colony performance. Bees that perform hygienic behaviour (hygienic bees) in our H colonies were more persistent in the hygienic activities throughout the days of the investigation. Such bees were more efficient in the removal of pin-killed brood than hygienic bees in the NH colonies. Hygienic bees in the H colonies were also specialist in the sub-tasks involved in the detection of odour stimulus from dead brood and continued to perform these activities throughout the days of the investigation (temporal persistence). Age-distribution of hygienic bees in the H colonies was asymmetrical, with a larger proportion of these bees performing hygienic activities early in life. At a colony-level, H showed higher efficiency compared to the NH colonies. The present results highlight the fact that individual behaviour may influence the collective dynamics of the hygienic behaviour in honeybee colonies. The results also note that the selection for highly hygienic colonies would result in changes in individual bees that improve the performance of the behaviour at the colony level. The relevance of task-partitioning and age-specialisation of hygienic bees on social immunity is discussed. [ABSTRACT FROM AUTHOR]

Fruit size in kiwifruit depends mainly on the number of seeds or fertilized ovules. Pollination by wind is not enough to obtain a fruit size accepted by the market, so it is necessary to perform this practice by means of bees and/or artificially. Since there are controversies regarding the efficiency of each practice, the objective of this study was to determine the effect of pollination with honey bees, artificial-hand pollination and the combination of both methods, on fruit set at harvest, fruit size (length, minor and major diameter, weight), number of seeds, maturity and internal quality indexes (color, firmness, soluble solids content, dry matter, titratable acidity). The trial took place in the 2018/2019 and 2019/2020 seasons on a 'Hayward' kiwifruit orchard located in Miramar, in the southeast of Buenos Aires Province, Argentina. Treatments were applied in three uniform canes on 9 vines. Honey bee and combined pollination treatments showed a better fruit set at harvest (24.6% and 18.5% higher, respectively) compared to artificial-hand pollination. Also, the fruits originated from honey bee pollinated flowers and the combined treatment had higher length (4.8% and 5.4%), major diameter (5.5% and 5.7%), minor diameter (2.9% and 3.5%), weight (14.6% and 15.3%) and number of seeds (11.6% and 10.4%) with respect to those obtained by artificial-hand pollination. No significant differences were detected between treatments for maturity/quality indexes at harvest. Pollination with honey bees and the combined method were the most efficient. These results indicate that proper management of honey bees for kiwifruit pollination improves yield without affecting maturity, and preserving fruit quality indexes. • Compare honey bee pollination, artificial-hand pollination and its combination. • Honey bee pollination and the combined method increase the number and size of fruits in kiwifruit. • Pollination with honey bees preserves fruit maturity and quality. • Pollination with honey bees needs an appropriate management protocol in kiwifruit. [ABSTRACT FROM AUTHOR]

NMOSD is an inflammatory disorder of the central nervous system that primarily affects the optic nerves and spinal cord. Rituximab (RTX) is a monoclonal antibody directed against CD20, an epitope expressed on pre-B and mature B cells. It has of wide use in several antibody-mediated autoimmune diseases. To demonstrate RTX clinical efficacy at different initial and maintenance doses administered in patients with NMOSD. In this retrospective/observational study we recruited subjects with NMOSD with at least one RTX infusion. Annual relapse rates (ARR) were compared in several induction and maintenance regimens with RTX in 66 patients with NMOSD. Fifty-four (81.8%) were female and two thirds (66.7%) had positive anti-AQP4 antibodies. The most prevalent induction and maintenance regimens were 1000 mg on days 1 and 15 (51.5%) and 1000 mg every 6 months (40.9%), respectively. Overall, the annual relapse rate (ARR) decreased from 1.15 to 0.46 with RTX (p < 0.001). In patients with persistent relapses, the ARR decreased from 1.66 to 1.22, representing a relative risk reduction of 24%. Treatment with RTX decreased the ARR from 1.36 to 0.4 in the 500 mg induction and maintenance dose subgroup, and from 0.7 to 0.4 in the 1000 mg induction and maintenance dose subgroup. RTX treatment in patients with NMOSD demonstrated a marked and sustained reduction in the ARR, regardless of induction and maintenance regimens. EDSS stability was observed, even in patients with active and severe NMOSD. • Rituximab is an effective therapy in AQP4 NMOsd patients. • Relapse rate decreased from 1.15 ± 1.18 to 0.46 in patients treated with Rituximab. • In patients with persistent relapses, we found a 24% decrease in the relative risk of relapse. • The benefit seems to be maintained regardless of the initial and maintenance od RTX dose. [ABSTRACT FROM AUTHOR]