1. Chronic Arsenic Exposure Impairs Macrophage Functions in the Exposed Individuals.
- Author
-
Nilanjana Banerjee, Saptarshi Banerjee, Rupashree Sen, Apurba Bandyopadhyay, Nilendu Sarma, Papiya Majumder, Jayanta Das, Mitali Chatterjee, Syed Kabir, and Ashok Giri
- Subjects
- *
PHYSIOLOGICAL effects of arsenic , *MACROPHAGES , *CELL culture , *IMMUNOSUPPRESSION , *CELL adhesion , *BLOOD cells , *NITRIC oxide , *GENE expression - Abstract
Abstract Introduction Owing to the established roles of human macrophages in immune defense, we investigated the effect of chronic arsenic exposure upon these major hematopoietic cells in 70 arsenic-exposed individuals with skin lesions and 64 unexposed individuals. Methods Human monocyte-derived macrophages were prepared from peripheral blood mononuclear cells, by culture of the adherent cells for 6 days in medium supplemented with granulocyte–monocyte colony stimulating factor. Parameters studied included cell adhesion capacity, expression of CD54 and F-actin, nitric oxide production, phagocytic capacity, and effect of arsenic on Rho A-ROCK pathway. Results In macrophages of exposed individuals when compared to unexposed group, there was cell rounding accompanied with a significant (p Discussion and Conclusion These altogether could contribute significantly to arsenic-induced immunosuppression observed in the arsenic-exposed individuals. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF