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The time to achieve good metabolic control after diagnosis is essential for type 2 diabetes patients because it can influence long-term prognosis. This study aimed to elucidate the predictive role of several clinical and organization factors in normalizing metabolism within 6 months. A multi-centered, retrospective, observational study on 960 patients, with diabetes duration of 12 months or less, consecutively seen in 123 Italian clinics, was undertaken. Information about clinic’s organization, along with data abstracted from medical records at enrollment (first visit) and after 6 months (follow-up visit), was collected. At 6 months, HbA1c dropped by -3.1 ± 2.2 points in those who achieved HbA1c <7 % (responders), whereas in non-responders (HbA1c ≥7 %), the mean reduction was -1.8 ± 1.9. The intervention markedly reduced lipids, blood pressure, BMI, and waist circumference, especially in responders. The presence of a diabetes team correlated with a likelihood of HbA1c normalization (OR 1.94, 1.17–3.22). By contrast, indicators of advanced disease such as previous retinopathy (0.53, 0.29–0.98), use of secretagogues (0.40, 0.25–0.64), high levels of HbA1c at first visit and related insulin use emerged as adverse factors. Early detection of diabetes, along with human resources and organization, was found to play a crucial role in rapidly attaining good metabolic control. [ABSTRACT FROM AUTHOR]

Abstract: Atriplex halimus is found in the Mediterranean Basin along the coastal areas of Sardinia, but few data are available on its adaptability to salinity. The effects of drought and salinity under controlled conditions on two clones of A. halimus, designated MOR2 and SOR4, originating from southern and northern Sardinia, respectively, were compared with those of seedlings of A. nummularia, an Australian species widely used in the restoration of arid areas. The effects of increasing NaCl salinity above seawater concentrations and of increasing the KCl concentration gradient were tested. Plants were harvested and analysed after 10 and 20 days of NaCl and KCl treatments. All plants remained alive until the end of treatment, although growth was strongly reduced, mainly for the A. halimus MOR2 clone, under increasing concentrations of KCl. The leaves and roots of both species responded positively to increasing NaCl concentrations up to 600mM NaCl for A. halimus, whereas the optimal growth of A. nummularia was recorded at 300mM NaCl. SOR4 was more sensitive to KCl toxicity. The Na+ concentration in the plants increased with increased salinity and was higher in A. halimus than in A. nummularia, suggesting that A. halimus is an ion accumulator and may be used for phytoremediation. The sodium accumulation in the roots of the A. halimus MOR2 clone was far greater than in its leaves. This suggests that MOR2 is an Na+ excluder, either by minimising the entry of salt into the plant or by an excretion mechanism via the vesiculated hairs that play a significant role in the removal of salt from the remainder of the leaf, thereby preventing its accumulation to toxic levels in the leaves, whereas SOR4 acted as an Na+ includer. Higher levels of proline were detected in the MOR2 clone under NaCl treatments, suggesting a more developed adaptative mechanism for the selection of this characteristic in the southern part of the island, which is more exposed to abiotic stresses, particularly water stress that is either generated by salinity or by other causes. [Copyright &y& Elsevier]

In this work we present and discuss the design of an array of soft-sensors to estimate the nitrate concentration in the denitrifying post-filtration unit at the Viikinmäki wastewater treatment plant in Helsinki (Finland). The developed sensors aim at supporting the existing hardware analyzers by providing a reliable back-up system in case of malfunction of the instruments. In the attempt to design easy to implement and interpretable sensors, computationally light linear models have been considered. However, due to the intrinsic nonlinearity of the process, also nonlinear but still computationally affordable models have been considered for comparison. The experimental results demonstrate the potential of the developed soft-sensors and the possibility for an on-line implementation in the plant's control system as alternative monitoring devices. [ABSTRACT FROM AUTHOR]

Alterations in plasma lipid profile and in intracellular cholesterol homoeostasis have been described in various malignancies; however, significance of these alterations, if any, in cancer biology is not clear. The aim of the present study was to investigate a possible correlation between alterations in cholesterol metabolism and expansion of leukaemia cell numbers. Lipid profiles in plasma and in primary leukaemia cells isolated from patients with acute or chronic lymphocytic leukaemia (ALL and CLL) were studied. Decreased levels of HDL-C were observed in plasma of leukaemic patients, levels of total cholesterol, LDL-C, triglycerides and phospholipids were unchanged or only slightly increased. As compared to normal lymphocytes, freshly isolated leukaemic cells showed increased levels of cholesterol esters and reduction in free cholesterol. Growth stimulation of ALL and CLL cells with phytohemagglutinin led to further increase in levels of cholesterol esters. Conversely, treatment with an inhibitor of cell proliferation such as the mTOR inhibitor, RAD, caused decline in population growth rate of leukaemia cells, which was preceded by sharp reduction in rate of cholesterol esterification. On the other hand, exposure of leukaemic cells to two inhibitors of cholesterol esterification, progesterone and SaH 58-035, caused 60% reduction in their proliferation rate. In addition to demonstrating tight correlation between cell number expansion and cholesterol esterification in leukaemic cells, these results suggest that pathways that control cholesterol esterification might represent a promising targets for novel anticancer strategies. [ABSTRACT FROM AUTHOR]

Nine cultivars of myrtle (Myrtus communis) were analyzed for their leaf essential oil yield and composition during two seasons. The oil yield composition on leaf dry weight basis varied with the year of collection and the season ranging from 0.6 to 10.7 g·kg-1 in the different cultivars. The oils were analyzed by GC-MS for compound identification and by GC for quantitative determinations by comparison with authentic standards. The main components of the essential oils were α-pinene (366.8 to 816.8 g·kg-1); limonene (42.8 to 468.8 g·kg-1), 1,8-cineole (32.0 to 230.6 g·kg-1), linalool (0.8 to 110.3 g·kg-1), geranyl-acetate (12.4 to 99.2 g·kg-1). Other minor components were α-terpineol (1.6 to 44.4 g·kg-1), terpinolene isomer 1 (0.0 to 42.6 g·kg-1), linalyl-acetate (0.0 to 36.3 g·kg-1), α-humulene (0.0 to 19.6 g·kg-1), terpinolene-isomer 2 (0.0 to 14.9 g·kg-1), p-cymene (0.0 to 13.0 g·kg-1) and geraniol (0.0 to 12.4 g·kg-1). The total content of the other minor components was less than 10 g·kg-1. All cultivars were suitable for hydro-alcoholic infusion production and, in spite of the minor oil yield, the leaves harvested in winter showed an essential oil composition quite similar to those from spring. [ABSTRACT FROM AUTHOR]

Abstract: The chemical composition of fruit belonging to ‘Barbara’ and ‘Daniela’ myrtle cultivars was monitored during development from fruit-set to an over-ripe stage (July–January), with the aim to identify a reliable maturity index. Acidity, pH, reducing and total sugars, phenols, tannins, anthocyans, carbon dioxide and ethylene production rates were monitored over two different year seasons. Titratable acidity decreased during maturation, with significant differences due to cultivar and year of observation. Reducing sugars increased in both cultivars approximately sevenfold from fruit set to complete maturation. Total sugar content increased similarly ranging from 1.43% and 1.41% at fruit set to 8.28% and 7.56% at maturation for ‘Barbara’ and ‘Daniela’, respectively. Total phenols and tannins occurred at high levels after fruit set and declined during development. Anthocyans levels increased, in both cultivars, according to a sigmoid curve. The pattern of respiration rate showed a gradual decline in both cultivars ranging from 365.81 and 396.42mgkg−1 h−1 to 79.98 and 52.27mgkg−1 h−1, respectively for ‘Barbara’ and ‘Daniela’ in 2006. A peak of variable size was observed in October–November period. Small increases in ethylene production have been detected during fruit development ranging from 130.57 and 269.14μLkg−1 h−1 measured at the onset of development to 13.04 and 19.36μLkg−1 h−1 measured at harvest for ‘Barbara’ and ‘Daniela’, respectively. [Copyright &y& Elsevier]

The present work describes an efficient computational method for the simulation of turbulent flames. The main three ingredients of the method are Roe's schemes, the Spalart-Allmaras one-equation turbulence model, and a general preconditioning technique. These three ingredients are well known to ensure accuracy, robustness, and generality. The solution of the conservation equations is fully coupled, and the method is implemented in a three-dimensional parallel implicit solver. This potentially allows the use of the same mathematical method for the analysis of turbulent reactive flows at all speeds. The choice of the Spalart-Allmaras model, as opposed to typical two-equation models requires particular attention on the derivation of the micromixing time. Such a derivation is of fundamental importance, for the micromixing time represents the base upon which chemical source terms are built for many combustion models. Deriving a correct model for the micromixing time from the only resolved turbulent quantity kinematic eddy viscosity is challenging. To test the method, two low-Mach-number (0.1-0.2) turbulent reactive flows are simulated using the eddy dissipation model, and the results are compared with both experiments and previous numerical studies. It is established that the proposed method leads to satisfactory results. [ABSTRACT FROM AUTHOR]

The zoonotic nematode Trichinella britovi has been documented in animals and/or humans of the Mediterranean islands of Corsica and Sardinia since 2004. From 2005 to 2007 in the Sardinia island, several surveys had shown that T. britovi was circulating among backyard and free-ranging pigs reared in the Orgosolo municipality but all attempts had failed to detect this parasite in wild susceptible animals. The aim of the present work was to investigate the circulation of T. britovi in pigs and wildlife of the Orgosolo municipality, and of surrounding municipalities and provinces in the 2010–2014 slaughtering/hunting seasons. The results show that the T. britovi circulation was still restricted to the Orgosolo municipality with a prevalence of 2.6% in free-ranging pigs and 0.2% in backyard pigs but, for the first time, this parasite was detected also in 0.4% of wild boar, and 27.6% of red foxes. No infection was detected in backyard pigs, wild boar, and red foxes of the other municipalities and provinces. Since 1978, African swine fever is endemic in Sardinia and foci of this virus are still active in the investigated areas favoring cannibalism and, consequently, the T. britovi transmission, due to the high mortality rate caused by this virus. This is the first documented report on the transmission of T. britovi between the domestic and the sylvatic cycle. The health authority of the island must provide a service to dispose animal carcasses and offal, stamping out illegal free-ranging pigs, and train hunters and pig owners to manage waste and by-products according to the EU regulations. [ABSTRACT FROM AUTHOR]

Abstract: Rheomorphic structures in welded ignimbrites are commonly associated with deposition from hot pyroclastic flows on inclined topography or by tractional shear on aggrading agglutinate by the pyroclastic density current. We describe a type of rheomorphic deformation involving the formation of diapirs in ignimbrite following the re-equilibration of internal gravitational instabilities within the deposit. We show that diapirs can develop in horizontal welded ignimbrites that have an inverted density stratification. The interpretation of these structures in terms of their dynamics and kinematics can help in defining the timing of the deformation history of ignimbrites. The rhyolitic Serra di Paringianu Ignimbrite is the uppermost, largest ignimbrite of Cenozoic volcanism in SW Sardinia. A detailed study at La Punta, north of San Pietro island (Sardinia; Italy), schematically characterized by a twin sequence of densely welded and partially welded ignimbrite flow units of the Serra di Paringianu Ignimbrite, revealed the presence of mushroom and pillow-shaped diapiric structures, constituted by material of an intermediate, partially welded, flow unit intruding into an upper, densely welded, unit. The diapirs are generally connected with their source region and show both vertical and lateral variations in the textural and physical features. The uppermost densely welded unit is deformed within a few meters of the diapiric structures, with the foliation pattern of the surrounding ignimbrite enveloping the cup region of diapirs. We used the existing numerical models of cooling–compaction of welded ignimbrites to constrain the time scale for the development of specific rheomorphic structures. We present a model in which the diapirism involves the buoyant rise of partially welded lithofacies into the densely welded cover in a time scale of a few months. The diapir model takes into account the rheology of the host and intruding layers and explains the textural and physical modifications both experienced by the partially welded lithofacies during rise and induced in the host layers. The model shows that the time scale of the diapiric rise well agrees with the time scale of the cooling process of the upper portion of the ignimbrite. [Copyright &y& Elsevier]

Background: Hepatic surgery is the treatment of choice for resectable colorectal liver metastases. Neoadjuvant chemotherapy can downstage the tumour and enable surgery in up to 38 per cent of patients whose tumours were initially considered irresectable. Methods: This prospective study included 150 patients who underwent hepatic resection over a 4-year period. One hundred and sixteen patients had resection without neoadjuvant chemotherapy (group 1) and 34 had chemotherapy before liver surgery. (group 2). Perioperative features, survival and pattern of recurrence were compared. Results: Median follow-up was 35.1 months. The in-hospital mortality rate was zero. Three-year overall survival in the two groups was comparable (P = 0.232). The 3-year disease-free survival rate was 21 per cent in the neoadiuvant group compared with 50.5 per cent in the immediate resection group (P < 0.001). Recurrence rates were 94 per cent (32 of 34) in group 1 and 66.4 per cent in group 2 (P = 0.001); extrahepatic recurrence, alone or associated with recurrence in the liver, was significantly more common in group 2 than in group 1 (78 versus 55 per cent; P = 0.016). Multivariate analysis revealed that resection of the recurrence was the most important independent prognostic factor for improved disease-free survival (relative risk 0.2; P < 0.001). Patients in group 2 had reduced disease-free survival (RR 1.8; P = 0.012). Conclusion: The recurrence rate among patients who had neoadiuvant chemotherapy and surgery for initially irresectable liver metastases was extremely high. Re-resection should be attempted whenever feasible. [ABSTRACT FROM AUTHOR]

Recent studies into the estimation and control of an activated sludge process (ASP) at a wastewater treatment plant suggest that artificial-intelligence methods, such as neural networks, fuzzy systems and genetic algorithms, can improve the plant performance in terms of reduced operation costs and improved effluent quality. In this paper, a neural-network-based soft sensor is developed for the on-line prediction of effluent concentrations in an ASP in terms of primary hard-to-measure variables, such as chemical oxygen demand, total nitrogen content and total suspended solids, starting from secondary on-line easy-to-measure variables, such as oxygen and nitrogen compound concentrations in biological tanks, input flow rate and alkalinity, among others. An algorithm based on principal component analysis is applied to select the optimal net input vectors for the soft sensor, using an appropriated number of samples of the secondary variables set. The proposed soft sensor is tested on the ASP of a large-scale municipal wastewater treatment plant running under the GPS-X simulation frame and validated with operational gathered data. Satisfactory low values for mean and maximum absolute prediction errors are obtained, even when high values of sampling time of primary variables are set, as it is frequently done during monitoring operation. In this way, data-driven soft-sensors based on neural networks can become valuable tools for plant operators for the recognition of operational states in terms of low cost and efficient prediction of primary process variables such as chemical oxygen demand, total nitrogen content and total suspended solids, therefore avoiding the acquisition of expensive and sometimes unreliable instruments for measuring nutrient concentrations in plant. [ABSTRACT FROM AUTHOR]

An acute outbreak of Taenia hydatigena cysticercosis, causing mortality in 5 of 21 (23.8%) female lambs, is reported. Gross post-mortem examinations and histology showed Cysticercus tenuicollis as the cause of death. Biochemical parameters in infected lambs confirmed severe hepatitis. Praziquantel, given once at 15 mg/kg body weight (bw), was administered and a dramatic improvement in the clinical condition and biochemical parameters was observed up to 30 days following treatment. [ABSTRACT FROM AUTHOR]

Mt. Vesuvius is one of the most studied volcanoes in the world and its proximity to an extremely populated area makes it also one of the most threatening. Violent Strombolian (VS) events have occurred in the most recent history of the volcano, and they are the type most likely to occur in case of reactivation of the volcano in the near future. In order to investigate the dynamics and hazard of this type of eruption, we performed new field and laboratory work and numerical simulations of plume dynamics, fallout and tephra dispersal associated with such eruptions. Attention was specifically focused on the 1906 eruption, a recent and well-studied VS event. Based on new field analyses and historical observations, we reconstructed the temporal evolution of eruption source conditions during the event. The reconstructed explosive phase of the eruption is inferred to have been 8 days long and characterized by two distinct stages: a former short and intense period with sustained convective plume fed by powerful lava fountains (20 h) followed by a prolonged and less intense period of ash emission (172 h). The total grain-size distributions for both phases, used as inputs to the model, were obtained by field work and laboratory analyses. Based on these new volcanological data and reconstruction of wind field direction and intensity, partially derived from historical sources, the 1906 event was numerically simulated and results compared to deposit distributions. The modelling outcomes for the ash emission phase provide a better agreement with the measured tephra mass load for a simulation run in which ash aggregation (described by the models of Cornell et al. J Volcanol Geotherm Res 17:89-109, , and Biass et al. Nat Hazard Earth Syst Sci 14:2265-2287, ) is specifically taken into account, confirming the importance of this process during tephra dispersal. The aggregation model that best fits the simulated results to the measured ground loadings has 80 % of particles Φ ≥ 4 that aggregate uniformly in the range Φ = −1 to 3. Two additional simulations of a VS event were carried out by using meteorological data of two specific periods to exemplify weather's potential on impacts of such eruptions, particularly tephra loading, on the surrounding areas. The model outcomes clearly highlight the major effects of differences in local meteorology on plume dynamics and ash dispersal and the key role of wind shear in determining the cumulative thickness of ground deposits. Results also show that, due to the long duration of this kind of eruption and the large variability in zonal winds at this latitude, ash dispersal and fallout from VS events at Vesuvius represent a probable hazard for all of the territory near Vesuvius, including the city of Naples, where cumulative tephra loadings might reach critical thresholds for roof collapse and infrastructure damage. [ABSTRACT FROM AUTHOR]

Three consulting teams conducted independent modelling projects for three different wastewater treatment plants ranging in size from approximately 113,800m³/d (30 mgd) to 530,000m³/d (140 mgd), in different parts of the world (USA and Finland). The plants have different treatment objectives ranging from nitrification and partial denitrification (nitrate plus nitrite ,8.7 mg/L) to enhanced nutrient removal (total nitrogen ,3 mg/L, total phosphorus ,0.3 mg/L). Commonlyused models were applied in the case studies, including ASM3 (using the GPS-X simulator), New General (using GPS-X), Dold (using BioWin), and a variation of the Dold model methanol degradation capabilities (NGmeth within GPS-X). The authors compare and contrast the modelling approaches taken, including calibration and validation approaches, sensitivity analyses, and the application of results to full-scale studies, designs and operations. Despite several differences between the approaches, there are many similarities which are discussed in light of the IWA draft uniform protocol for activated sludge modelling. The authors also discuss current modelling limitations and offer suggestions to improve the state of the art. [ABSTRACT FROM AUTHOR]

Abstract. A positive correlation between cholesterol esterification and growth rate potential was previously found in our laboratory during the growth of CEM and MOLT4 lymphoblastic cells. In the current study, we investigated whether the rates of cholesterol esters synthesis correlate with changes of acyl-CoAcholesterol acyltransferase (ACAT) mRNA levels and of other genes implied in cholesterol biosynthesis and uptake, such as 3-hydroxy-3-methylglutaryl-CoA (HMGCoA) reductase and low density lipoprotein (LDL) receptor. The results showed that the more rapid growing CEM cells had lower levels of expression of HMGCoA-reductase and LDL receptors compared to MOLT4. By contrast, ACAT mRNA levels were higher in CEM cells, further supporting the concept of a possible involvement of cholesterol esters in the regulation of cell growth and division. In this study, high levels of cholesterol esterification and of expression of ACAT gene were also associated with a markedly increased expression of multidrug resistance (MDR1) gene, suggesting that MDR1 activity might contribute to regulate the rate of cell growth and division by modulating intracellular cholesterol ester levels. [ABSTRACT FROM AUTHOR]

The ascites hepatoma Yoshida AH130 causes in the host a rapid and progressive body weight loss, associated with reduced food intake, and protein and lipid hypercatabolism. Because insulin regulates glucose as well as lipid and protein metabolism, we suggest that the observed alterations are at least in part secondary to hypoinsulinemia and/or to the increase of counterregulatory hormones in AH130-bearing rats. To verify this hypothesis, controls with free access to food (n = 4), controls with free access to food plus insulin (107 micromol. kg body wt-1. d-1) (n = 4), controls pair-fed to the tumor-bearing rats (n = 4), pair-fed controls treated with insulin (n= 4), tumor hosts (n = 9), and tumor hosts treated with insulin (n = 6) were used. The Yoshida ascites hepatoma cells ( approximately 10(8) cells/rat) were inoculated intraperitoneally. Daily food intake and body weight were measured; insulin was injected starting the day of tumor implantation for 6 d. The metabolism of both cholesterol and lipids was investigated in tumor cells, and ascitic fluid and blood serum were investigated at the end of treatment. Insulin prevented the reduction of food intake (19 +/- 0.6 vs. 13 +/- 0.4 g/d, P < 0.01; AH130 hosts treated and not treated with insulin, respectively), the loss of body weight (202 +/- 12 vs. 135 +/- 9 g, P < 0.01), lowered the circulating triglycerides (48.3 +/- 4.9 vs. 84.5 +/- 7.1 mmol/L, P < 0.01), and free fatty acids (561 +/- 47 vs. 989 +/- 54 mmol/L (P < 0.01), while corrected the decrease of adipose lipoprotein lipase activity (1,240 +/- vs. 300 +/- pmol FA, P < 0.01) observed in AH130 hosts. Moreover, insulin prevented the decrease in HDL cholesterol (13.2 +/- 0.8 vs. 9.3. +/- 0.7 mmol/L, P < 0.01) and significantly increased hepatic cholesterol synthesis as evaluated by 14C-acetate incorporation into cholesterol, in both liver (3,337 +/- 245 vs. 830 +/- 115 Bq/g, P < 0.01) and AH130 cells (11,676 +/- 1,693 vs. 4,196 +/- 527 Bq/10(6) cells, P < 0.01). Thus insulin treatment ameliorated many metabolic derangements, with a lengthening of rats survival time (7 +/- 1 vs. 11 +/- 1 d, P < 0.05) without significantly stimulating tumor growth. These data, together with our previous observations on the effectiveness of insulin on protein turnover perturbations, suggest that many metabolic alterations occurring during cancer cachexia can be avoided by the administration of this hormone. [ABSTRACT FROM AUTHOR]